{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T10:51:39Z","timestamp":1769597499274,"version":"3.49.0"},"reference-count":65,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evandro Chagas Institute, Department of Health and Environmental Surveillance, Ministry of Health and Brazilian National Council for Scientific and Technological Development (CNPq)","award":["314522\/2021-2"],"award-info":[{"award-number":["314522\/2021-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>To investigate the association between miscarriage and ZIKV and CHIKV infection. The study population consisted of pregnant women who had miscarriages between 2015, 2016 and 2017, comprising a total of 30 women who were treated at the Santa Casa de Miseric\u00f3rdia do Par\u00e1 Foundation (FSCMPA). The processed samples came from already paraffinized material containing placental and fetal remains, where they were tested with hematoxylin\u2013eosin and immunohistochemistry for ZIKV and CHIKV. Regarding the sociodemographic, clinical and obstetric characteristics of the patients, they correspond to the age group between 20 and 29 years of age; of brown color; women who had abortions for the first time; miscarriages occurring in the first trimester of pregnancy; women belonging to the metropolitan region of Bel\u00e9m; diagnosed with incomplete abortion and who had undergone uterine curettage procedure. Regarding the histopathologic and immunohistochemical findings, an inflammatory infiltrate rich in neutrophils and lymphocytes, among others, was found in the endometrial fragments and chorionic membranes. In addition, placental areas consisting of edema, necrosis and hemorrhage were found. The study identified ZIKV and CHIKV in 40% (n = 12) of samples from spontaneous abortion specimens, and CHIKV was the most prevalent virus in the study, representing 36.11% of the total specimens, with reddish granular material in the cytoplasm of decidua cells and placental villi suggesting that the viruses may be present in these regions of the placenta.<\/jats:p>","DOI":"10.3390\/microorganisms13030678","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T07:20:00Z","timestamp":1742282400000},"page":"678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of Zika and Chikungunya Viruses on Spontaneous Abortions: Insights from a Reference Maternity Hospital"],"prefix":"10.3390","volume":"13","author":[{"given":"Anne Kerollen Pinheiro","family":"de Carvalho","sequence":"first","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-9346","authenticated-orcid":false,"given":"Ana Cec\u00edlia Ribeiro","family":"Cruz","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6267-9966","authenticated-orcid":false,"given":"Juarez Ant\u00f4nio Sim\u00f5es","family":"Quaresma","sequence":"additional","affiliation":[{"name":"Department of Pathology, State University of Par\u00e1, Bel\u00e9m 66075-110, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9153-1234","authenticated-orcid":false,"given":"Arnaldo Jorge Martins","family":"Filho","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Patologia, Ananindeua 67140-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0123-6712","authenticated-orcid":false,"given":"Darlene de Brito Simith","family":"Durans","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlando Pereira","family":"Amador Neto","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Patologia, Ananindeua 67140-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia do Socorro Oliveira","family":"de Lima","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Patologia, Ananindeua 67140-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norma Suely de Carvalho Fonseca","family":"Assun\u00e7ao","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Santa Casa de Miseric\u00f3rdia do Par\u00e1, Bel\u00e9m 66030-010, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edna Cristina Santos","family":"Franco","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Patologia, Ananindeua 67140-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9712-8442","authenticated-orcid":false,"given":"Patr\u00edcia Braz\u00e3o","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Pathology, State University of Par\u00e1, Bel\u00e9m 66075-110, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3319-1854","authenticated-orcid":false,"given":"Eliana Vieira Pinto","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto Evandro Chagas, Se\u00e7\u00e3o de Arbovirologia e Febres Hemorr\u00e1gicas, Ananindeua 67030-000, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1590\/1806-93042020000200003","article-title":"Fatores associados ao aborto espont\u00e2neo: Uma revis\u00e3o sistem\u00e1tica","volume":"20","author":"Oliveira","year":"2020","journal-title":"Rev. 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