{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:01:45Z","timestamp":1791583305987,"version":"4.3.4"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32302242"],"award-info":[{"award-number":["32302242"]}]},{"name":"National Natural Science Foundation of China","award":["2022A1515110426"],"award-info":[{"award-number":["2022A1515110426"]}]},{"name":"National Natural Science Foundation of China","award":["2023B0202040002"],"award-info":[{"award-number":["2023B0202040002"]}]},{"name":"National Natural Science Foundation of China","award":["2022B1212010015"],"award-info":[{"award-number":["2022B1212010015"]}]},{"name":"Basic and Applied Basic Research Foundation of Guangdong Province","award":["32302242"],"award-info":[{"award-number":["32302242"]}]},{"name":"Basic and Applied Basic Research Foundation of Guangdong Province","award":["2022A1515110426"],"award-info":[{"award-number":["2022A1515110426"]}]},{"name":"Basic and Applied Basic Research Foundation of Guangdong Province","award":["2023B0202040002"],"award-info":[{"award-number":["2023B0202040002"]}]},{"name":"Basic and Applied Basic Research Foundation of Guangdong Province","award":["2022B1212010015"],"award-info":[{"award-number":["2022B1212010015"]}]},{"name":"Guangdong Provincial Key Research and Development Program","award":["32302242"],"award-info":[{"award-number":["32302242"]}]},{"name":"Guangdong Provincial Key Research and Development Program","award":["2022A1515110426"],"award-info":[{"award-number":["2022A1515110426"]}]},{"name":"Guangdong Provincial Key Research and Development Program","award":["2023B0202040002"],"award-info":[{"award-number":["2023B0202040002"]}]},{"name":"Guangdong Provincial Key Research and Development Program","award":["2022B1212010015"],"award-info":[{"award-number":["2022B1212010015"]}]},{"name":"Key Laboratory Project of Guangdong Province","award":["32302242"],"award-info":[{"award-number":["32302242"]}]},{"name":"Key Laboratory Project of Guangdong Province","award":["2022A1515110426"],"award-info":[{"award-number":["2022A1515110426"]}]},{"name":"Key Laboratory Project of Guangdong Province","award":["2023B0202040002"],"award-info":[{"award-number":["2023B0202040002"]}]},{"name":"Key Laboratory Project of Guangdong Province","award":["2022B1212010015"],"award-info":[{"award-number":["2022B1212010015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The pathogenesis of ulcerative colitis (UC) has been fundamentally associated with intestinal microbiota dysbiosis and disruption of immune homeostasis. This study systematically investigates the therapeutic potential of Lactiplantibacillus plantarum HYY-S10 (HYY-S10), a novel strain isolated from De\u2019ang sour tea in Yun an, China, with a focus on its mechanisms for alleviating colitis through the modulation of gut microbiota. Using a dextran sulfate sodium (DSS)-induced colitis model in C57BL\/6J mice, our findings demonstrated that seven days of oral supplementation with HYY-S10 (1 \u00d7 108 CFU\/mL, 0.2 mL\/10 g body weight) significantly improved Disease Activity Index (DAI) scores and attenuated characteristic colitis symptoms, including progressive weight loss, rectal bleeding, and abnormal stool consistency. Administration of HYY-S10 exhibited significant immunomodulatory effects characterized by the downregulation of pro-inflammatory mediators (such as IL-1\u03b2, IL-6, IFN-\u03b3, and LPS) while concomitantly upregulating anti-inflammatory IL-10 expression. Additionally, the strain enhanced intestinal antioxidant capacity by increasing GSH-Px activity, which collectively contributed to the reduction in intestinal inflammation. Furthermore, HYY-S10 demonstrated multifaceted protective effects by ameliorating oxidative stress through the restoration of redox homeostasis and modulation of gut microbial ecology. Probiotic intervention significantly increased short-chain fatty acids (SCFAs) production and notably enhanced the relative abundance of beneficial taxa, including Akkermansia and Ruminococcus_B, while restoring microbial diversity and ecological stability. Collectively, our results demonstrate that HYY-S10 alleviates experimental colitis by modulating the intestinal immune axis and microbiota composition, providing mechanistic insights to support its potential as a probiotic-based therapeutic strategy for UC.<\/jats:p>","DOI":"10.3390\/microorganisms13040840","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T10:47:41Z","timestamp":1744282061000},"page":"840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Alleviating Effect of Lactiplantibacillus plantarum HYY-S10 on Colitis in Mice Based on an Analysis of the Immune Axis in the Intestine"],"prefix":"10.3390","volume":"13","author":[{"given":"Mengna","family":"Li","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijian","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingbo","family":"Yao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4248-352X","authenticated-orcid":false,"given":"Xiangze","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4136-6044","authenticated-orcid":false,"given":"Yanyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ijpharm.2018.12.046","article-title":"Oral administration of colitis tissue-accumulating porous nanoparticles for ulcerative colitis therapy","volume":"557","author":"Chen","year":"2019","journal-title":"Int. J. Pharm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s12016-018-8690-3","article-title":"Allergic and Immunologic Perspectives of Inflammatory Bowel Disease","volume":"57","author":"Clarke","year":"2019","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_3","first-page":"E56","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies (vol 390, pg 2769, 2018)","volume":"396","author":"Ng","year":"2020","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Liu, Y.H., Liu, G., and Fang, J. (2024). Progress on the mechanisms of Lactobacillus plantarum to improve intestinal barrier function in ulcerative colitis. J. Nutr. Biochem., 124.","DOI":"10.1016\/j.jnutbio.2023.109505"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"G171","DOI":"10.1152\/ajpgi.00048.2015","article-title":"Homeostasis of the gut barrier and potential biomarkers","volume":"312","author":"Wells","year":"2017","journal-title":"Am. J. Physiol.-Gastrointest. Liver Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhou, J., Liu, J.L., Gao, Y.Y., Shen, L.W., Li, S., and Chen, S.M. (2021). miRNA-Based Potential Biomarkers and New Molecular Insights in Ulcerative Colitis. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.707776"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1053\/j.gastro.2020.04.074","article-title":"Single-Cell Analyses of Colon and Blood Reveal Distinct Immune Cell Signatures of Ulcerative Colitis and Crohn\u2019s Disease","volume":"159","author":"Mitsialis","year":"2020","journal-title":"Gastroenterology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1093\/advances\/nmaa104","article-title":"Roles of the Polyphenol-Gut Microbiota Interaction in Alleviating Colitis and Preventing Colitis-Associated Colorectal Cancer","volume":"12","author":"Zhao","year":"2021","journal-title":"Adv. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7352","DOI":"10.3168\/jds.2023-23251","article-title":"Lactiplantibacillus plantarum ZJ316\u2013fermented milk ameliorates dextran sulfate sodium\u2013induced chronic colitis by improving the inflammatory response and regulating intestinal microbiota","volume":"106","author":"Gu","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_10","first-page":"1181","article-title":"Oral administration of Lactobacillus plantarum 06CC2 prevents experimental colitis in mice via an anti-inflammatory response","volume":"21","author":"Tanaka","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Min, F.F., Hu, J.L., Zhong, Y.D., Huang, T., Huang, Y.S., Nie, S.P., Xiong, T., and Xie, M.Y. (2023). Lactobacillus plantarum NCU1125 mitigates intestinal barrier injury induced by cyclophosphamide in mice. Food Biosci., 53.","DOI":"10.1016\/j.fbio.2023.102726"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"106167","DOI":"10.1016\/j.jff.2024.106167","article-title":"Beneficial and immunomodulatory effects of heat-killed Lactobacillus plantarum L137 in normal and acute colitis mice","volume":"116","author":"Wong","year":"2024","journal-title":"J. Funct. Foods"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yuan, Z.W., Yang, L.H., Zhang, X.S., Ji, P., and Wei, Y.M. (2020). Therapeutic effect of n-butanol fraction of Huang-lian-Jie-du Decoction on ulcerative colitis and its regulation on intestinal flora in colitis mice. Biomed. Pharmacother., 121.","DOI":"10.1016\/j.biopha.2019.109638"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"121669","DOI":"10.1016\/j.carbpol.2023.121669","article-title":"Structural characteristics of steamed Polygonatum cyrtonema polysaccharide and its bioactivity on colitis via improving the intestinal barrier and modifying the gut microbiota","volume":"327","author":"Gong","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1111\/ijfs.17397","article-title":"Impact of different lactic acid bacteria on nitrite degradation and quality of fermented carrot","volume":"59","author":"Li","year":"2024","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Shi, K., Yu, F.Z., Li, A.Z., Wang, Y.J., and Sun, W.C. (2024). Corrigendum to \u201cThe protective effect of Okanin on Colitis induced by dextran sulfate sodium in mice\u201d [Food Bioscience, 57 (2024) 103527 FBIO_103527]. Food Biosci., 59.","DOI":"10.1016\/j.fbio.2023.103527"},{"key":"ref_17","first-page":"157","article-title":"MiR-98-5p expression inhibits polarization of macrophages to an M2 phenotype by targeting Trib1 in inflammatory bowel disease","volume":"67","author":"Peng","year":"2020","journal-title":"Acta Biochim. Pol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Liu, Y., Luo, J., Liu, Y., Yu, S.Y., and Liu, J. (2023). Rheum tanguticum polysaccharide alleviates DSS-induced ulcerative colitis and regulates intestinal microbiota in mice. Food Biosci., 53.","DOI":"10.1016\/j.fbio.2023.102788"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3326","DOI":"10.1021\/acs.jafc.0c07407","article-title":"Lychee (Litchi chinensis Sonn.) Pulp Phenolics Activate the Short-Chain Fatty Acid-Free Fatty Acid Receptor Anti-inflammatory Pathway by Regulating Microbiota and Mitigate Intestinal Barrier Damage in Dextran Sulfate Sodium-Induced Colitis in Mice","volume":"69","author":"Huang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ghotaslou, R., Nabizadeh, E., Memar, M.Y., Law, W.M.H., Ozma, M.A., Abdi, M., Yekani, M., Kadkhoda, H., Hosseinpour, R., and Bafadam, S. (2023). The metabolic, protective, and immune functions of Akkermansia muciniphila. Microbiol. Res., 266.","DOI":"10.1016\/j.micres.2022.127245"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Xie, J., Huang, Q., Xie, H.J., Liu, J., Tian, S.M., Cao, R.Y., Yang, M., Lin, J.Z., Han, L., and Zhang, D.K. (2023). Hyaluronic acid\/inulin-based nanocrystals with an optimized ratio of indigo and indirubin for combined ulcerative colitis therapy via immune and intestinal flora regulation. Int. J. Biol. Macromol., 252.","DOI":"10.1016\/j.ijbiomac.2023.126502"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1093\/ajcn\/nqab402","article-title":"Tolerability and SCFA production after resistant starch supplementation in humans: A systematic review of randomized controlled studies","volume":"115","author":"Sobh","year":"2022","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/s13073-020-00764-z","article-title":"A single early-in-life antibiotic course increases susceptibility to DSS-induced colitis","volume":"12","author":"Ozkul","year":"2020","journal-title":"Genome Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1093\/ibd\/izab277","article-title":"Excessive Apoptosis in Ulcerative Colitis: Crosstalk Between Apoptosis, ROS, ER Stress, and Intestinal Homeostasis","volume":"28","author":"Wan","year":"2022","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2200341","DOI":"10.1002\/mnfr.202200341","article-title":"Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis","volume":"66","author":"Chen","year":"2022","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ning, K., Shi, C., Chi, Y.Y., Zhou, Y.F., Zheng, W.W., Duan, Y.M., Tong, W.W., Xie, Q.H., and Xiang, H.Y. (2024). Portulaca oleracea L. polysaccharide alleviates dextran sulfate sodium-induced ulcerative colitis by regulating intestinal homeostasis. Int. J. Biol. Macromol., 256.","DOI":"10.1016\/j.ijbiomac.2023.128375"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"105694","DOI":"10.1016\/j.phrs.2021.105694","article-title":"Main active components of Jiawei Gegen Qinlian decoction protects against ulcerative colitis under different dietary environments in a gut microbiota-dependent manner","volume":"170","author":"Li","year":"2021","journal-title":"Pharmacol. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1038\/s41577-022-00685-5","article-title":"Intestinal barrier protection","volume":"22","author":"Minton","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jff.2017.03.045","article-title":"Strategies to promote abundance of Akkermansia muciniphila, an emerging probiotics in the gut, evidence from dietary intervention studies","volume":"33","author":"Zhou","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sultan, S., El-Mowafy, M., Elgaml, A., Ahmed, T.A.E., Hassan, H., and Mottawea, W. (2021). Metabolic Influences of Gut Microbiota Dysbiosis on Inflammatory Bowel Disease. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.715506"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.ijbiomac.2021.03.137","article-title":"Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism","volume":"181","author":"Guo","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Son, S.U., Nam, A.Y., Kim, S.M., Rho, Y., and Shin, K.S. (2022). Improvement effects of pectic polysaccharide isolated from Saururus chinensis leaves on dextran sulfate sodium-induced ulcerative colitis in BALB\/c mice. Food Biosci., 50.","DOI":"10.1016\/j.fbio.2022.102027"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/4\/840\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:11:38Z","timestamp":1760029898000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/4\/840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":32,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["microorganisms13040840"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13040840","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,7]]}}}