{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:52:53Z","timestamp":1787496773082,"version":"build-2736575974"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301078"],"award-info":[{"award-number":["82301078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["PKUSS20220101"],"award-info":[{"award-number":["PKUSS20220101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Foundation of Peking University School and Hospital of Stomatology","award":["82301078"],"award-info":[{"award-number":["82301078"]}]},{"name":"Research Foundation of Peking University School and Hospital of Stomatology","award":["PKUSS20220101"],"award-info":[{"award-number":["PKUSS20220101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The differences in microbiota between periodontitis and health have been extensively studied; however, knowledge about how the microbiota shifts from shallow to deep periodontal pockets remains limited despite its clinical importance in disease progres-sion and management. Patients diagnosed with stage III periodontitis commonly pre-sent varied probing depths (PD) within the same oral cavity, reflecting localized disease severity. This study aims to analyze the microbiome of subgingival plaques at various PDs in periodontitis patients. Subgingival plaques were collected from sixteen healthy subjects (health group) and periodontal pockets of sixteen stage III periodontitis pa-tients (PD 0\u20133 mm, PD 4\u20135 mm and PD 6\u20139 mm groups). A total of 64 subgingival plaque samples underwent 16S rRNA gene sequencing. The PD 6\u20139 mm group exhib-ited significantly higher alpha diversity than the health group, and distinct subgingival microbial community structures were observed in periodontitis patients, regardless of probing depth. The relative abundance of specific genera differed notably between health and periodontitis states; Corynebacterium and Cardiobacterium decreased, whereas Schaalia increased in shallow pockets (PD 0\u20133 mm) of periodontitis relative to the health group. Co-occurrence network analysis on the species level revealed that the PD 4\u20135 mm group had the most complex interspecies interactions, followed by the PD 6\u20139 mm and PD 0\u20133 mm groups. These findings indicate significant variations in mi-crobial diversity, composition, and interspecies interactions associated with periodon-tal health and periodontitis severity, highlighting their potential relevance for clinical diagnosis and targeted therapeutic strategies.<\/jats:p>","DOI":"10.3390\/microorganisms13040930","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T20:50:46Z","timestamp":1744923046000},"page":"930","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Subgingival Microbial Composition in Health and Periodontitis with Different Probing Depths"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5838-8691","authenticated-orcid":false,"given":"Jingyan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Geng","sequence":"additional","affiliation":[{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"},{"name":"Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"},{"name":"Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingqian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7401-1410","authenticated-orcid":false,"given":"Wenbin","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2276-9286","authenticated-orcid":false,"given":"Jing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China"},{"name":"National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/prd.12147","article-title":"Microbial colonization of the periodontal pocket and its significance for periodontal therapy","volume":"76","author":"Mombelli","year":"2018","journal-title":"Periodontology 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