{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:10:49Z","timestamp":1790266249651,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Italian Ministry of Health \u201cFondi Ricerca Corrente\u201d to IRCCS Sacro Cuore Don Calabria Hospital\u2014L1P7","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]},{"name":"EU funding within the MUR","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>In Italy, the epidemiology of tick-borne pathogens is still poorly characterized. This prospective study was conducted at the IRCCS Sacro Cuore Don Calabria Hospital in Negrar di Valpolicella (Verona), northeastern Italy, from 2018 to 2022. Ticks from asymptomatic individuals visiting the hospital after a recent tick bite were characterized using microscopy and tested for pathogens using molecular tests. A total of 317 ticks collected from 280 subjects were analyzed, with most identified as Ixodes species (95.6%), followed by Rhipicephalus spp. (0.6%) and Dermacentor spp. (0.3%). Molecular analysis was performed on 257 single ticks and 23 pooled samples. Overall, 15.4% tested positive for at least one pathogen. The most frequently detected pathogen was Borrelia spp. (n = 22, 7.8%), including B. afzeli (n = 8), B. miyamotoi (n = 6), B. valaisiana (n = 2), B. garinii (n = 2), Borrelia spp. (n = 2), B. burgdorferi sensu stricto (n = 1), and B. spielmanii (n = 1). Rickettsia spp. was detected in 20 samples (7.1%), comprising R. helvetica (n = 11), R. monacensis (n = 7), and Rickettsia spp. (n = 2). Other pathogens included Anaplasma phagocytophilum (n = 5, 1.8%), Babesia venatorum (n = 2, 0.7%), and tick-borne encephalitis virus (n = 1, 0.4%). This study calls for enhanced surveillance in the province of Verona to clarify these pathogens\u2019 clinical impact.<\/jats:p>","DOI":"10.3390\/microorganisms13050965","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T10:40:48Z","timestamp":1745404848000},"page":"965","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Surveillance of Tick-Borne Pathogens in Ticks from Humans in the Province of Verona, Italy (2018\u20132022): A Prospective Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6017-1181","authenticated-orcid":false,"given":"Lucia","family":"Moro","sequence":"first","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4935-0349","authenticated-orcid":false,"given":"Graziana","family":"Da Rold","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), SCT2-Belluno, 32100 Belluno, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Beltrame","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3203-0872","authenticated-orcid":false,"given":"Fabio","family":"Formenti","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4561-3447","authenticated-orcid":false,"given":"Cristina","family":"Mazzi","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ragusa","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2956-8997","authenticated-orcid":false,"given":"Salvatore","family":"Scarso","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2373-1549","authenticated-orcid":false,"given":"Ilenia","family":"Drigo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), SCT2-Belluno, 32100 Belluno, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Degani","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5739-3795","authenticated-orcid":false,"given":"Chiara","family":"Piubelli","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4482-1295","authenticated-orcid":false,"given":"Carlo Vittorio","family":"Citterio","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), SCT2-Belluno, 32100 Belluno, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-3892","authenticated-orcid":false,"given":"Francesca","family":"Perandin","sequence":"additional","affiliation":[{"name":"Department of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar di Valpolicella, VR, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1089\/vbz.2022.0068","article-title":"The Epidemiology of Lyme Borreliosis in Europe: An 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