{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:19:03Z","timestamp":1782897543359,"version":"3.54.5"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T00:00:00Z","timestamp":1745539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The skin microbiome is identified as one of the crucial factors in several pathological conditions, including its potential capacity in modulating cancer progression and response to treatment. A strong association of Bacilli and Betaproteobacteria classes and the Bacteroidetes phylum with melanoma is described in patients with cutaneous malignancies, while an imbalance of S. epidermidis and S. aureus is related to the progression of other skin cancers. In the present study, we characterized the microbial community in suspected lesions of 35 patients, classified, after histological analysis, as malignant melanoma lesions and benign non-melanoma lesions. Mirrored healthy skin were also included as negative control. No significant difference in alpha and beta diversity was observed when samples were categorized in four different groups (melanoma samples vs. contralateral healthy samples; melanoma samples vs. benign lesions; benign lesions vs. contralateral controls; melanoma controls vs. benign controls). The differential abundance analyses show that Corynebacterium urealyticum is more abundant in melanoma samples compared to their control, while Roseomonas gilardii is less abundant in melanoma. Staphylococcus massiliensis, Bacillus coagulans, Paracoccus yeei, Corynebacterium jeikeium, and Corynebacterium pyruviciproducens are present only in melanoma samples when compared with benign lesions.<\/jats:p>","DOI":"10.3390\/microorganisms13050992","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T11:47:41Z","timestamp":1745581661000},"page":"992","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating Skin Microbial Community in Malignant Melanoma Lesions"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1768-6017","authenticated-orcid":false,"given":"Michele","family":"Properzi","sequence":"first","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4729-577X","authenticated-orcid":false,"given":"Valentina","family":"Dimartino","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4233-0839","authenticated-orcid":false,"given":"Daniele","family":"Pietrucci","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2198-1947","authenticated-orcid":false,"given":"Carla","family":"Fontana","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4214-2812","authenticated-orcid":false,"given":"Claudia","family":"Rotondo","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Lembo","sequence":"additional","affiliation":[{"name":"Dermatologia Azienda Ospedaliera San Camillo Forlanini, 00152 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8388-9268","authenticated-orcid":false,"given":"Francesco","family":"Ricci","sequence":"additional","affiliation":[{"name":"Istituto Dermopatico dell\u2019Immacolata, IDI-IRCCS, FLMM, 00167 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Scatozza","sequence":"additional","affiliation":[{"name":"Istituto Dermopatico dell\u2019Immacolata, IDI-IRCCS, FLMM, 00167 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Di Lella","sequence":"additional","affiliation":[{"name":"Istituto Dermopatico dell\u2019Immacolata, IDI-IRCCS, FLMM, 00167 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8076-7217","authenticated-orcid":false,"given":"Francesco","family":"Messina","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Chillemi","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"},{"name":"Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3882-3996","authenticated-orcid":false,"given":"Barbara","family":"Bartolini","sequence":"additional","affiliation":[{"name":"National Institute for Infectious Diseases \u201cLazzaro Spallanzani\u201d\u2014IRCCS, 00149 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4243-2392","authenticated-orcid":false,"given":"Antonio","family":"Facchiano","sequence":"additional","affiliation":[{"name":"Istituto Dermopatico dell\u2019Immacolata, IDI-IRCCS, FLMM, 00167 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3836","DOI":"10.1111\/jocd.16557","article-title":"Dermatological Health in the Light of Skin Microbiome Evolution","volume":"23","author":"Haykal","year":"2024","journal-title":"J. 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