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Butantan, S\u00e3o Paulo, Brazil","award":["2019\/17488-2"],"award-info":[{"award-number":["2019\/17488-2"]}]},{"name":"Funda\u00e7\u00e3o Butantan, S\u00e3o Paulo, Brazil","award":["2019\/05466-4"],"award-info":[{"award-number":["2019\/05466-4"]}]},{"name":"Funda\u00e7\u00e3o Butantan, S\u00e3o Paulo, Brazil","award":["2016\/11541-0"],"award-info":[{"award-number":["2016\/11541-0"]}]},{"name":"Funda\u00e7\u00e3o Butantan, S\u00e3o Paulo, Brazil","award":["2022\/08985-5"],"award-info":[{"award-number":["2022\/08985-5"]}]},{"name":"Funda\u00e7\u00e3o Butantan, S\u00e3o Paulo, Brazil","award":["304445\/2021-5"],"award-info":[{"award-number":["304445\/2021-5"]}]},{"name":"Funda\u00e7\u00e3o Butantan, S\u00e3o Paulo, Brazil","award":["88887.940389\/2024-00"],"award-info":[{"award-number":["88887.940389\/2024-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Pathogenic bacteria of the genus Leptospira are the etiological agents of leptospirosis, a disease that affects humans and animals worldwide. Despite the increasing number of studies, the mechanisms of leptospiral pathogenesis remain poorly comprehended. In this study, we report various interactions of the LigA7\u2019-13\u2019 and LigB1\u2019-7\u2019 domains with host components. The LigA7\u2019-13\u2019 and LigB1\u2019-7\u2019 were cloned into the pET28a vector, and the recombinant proteins were expressed in E. coli C43 (DE3) and E. coli BL21 (DE3), respectively. Both recombinant protein domains were expressed in soluble form and purified using nickel-chelating chromatography. The rLigA7\u2019-13\u2019 and rLigB1\u2019-7\u2019 domains exhibited binding to several types of integrins, with most interactions occurring in a dose-dependent and saturable manner, consistent with the characteristics of typical receptor-ligand interactions. The recombinant domain LigA7\u2019-13\u2019 demonstrated affinity for the glycosaminoglycans (GAGs) chondroitin-4-sulfate, chondroitin sulfate, heparin, chondroitin sulfate B, and heparan sulfate, while no binding was detected for LigB1\u2019-7\u2019 with these molecules. Both rLigA7\u2019-13\u2019 and rLigB1\u2019-7\u2019 interacted with components of the terminal complement pathway and were capable of recruiting C9 from normal human serum (NHS). These interactions may inhibit the formation of polyC9, ultimately preventing the assembly of the membrane attack complex (MAC). Collectively, our data expand the repertoire of host components that interact with rLigA7\u2019-13\u2019 and rLigB1\u2019-7\u2019, opening new avenues for understanding leptospiral immune evasion and broadening the roles of these domains in bacterial virulence.<\/jats:p>","DOI":"10.3390\/microorganisms13061293","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T03:19:38Z","timestamp":1748834378000},"page":"1293","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Revisiting Host-Binding Properties of LigA and LigB Recombinant Domains"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7986-7423","authenticated-orcid":false,"given":"Henrique M.","family":"Pires","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9607-7066","authenticated-orcid":false,"given":"Igor R. M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-8312","authenticated-orcid":false,"given":"Aline F.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4851-0870","authenticated-orcid":false,"given":"Ana L. T. O.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,31]]},"reference":[{"key":"ref_1","unstructured":"Faine, S., Adler, B., Bolin, C., and Perolat, P. (1999). \u201cLeptospira\u201d and Leptospirosis, MediSci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A Zoonotic Disease of Global Importance","volume":"3","author":"Bharti","year":"2003","journal-title":"Lancet Infect. 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