{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:25:09Z","timestamp":1760059509331,"version":"build-2065373602"},"reference-count":121,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Campylobacter jejuni and Campylobacter coli are the two main campylobacter species that cause foodborne campylobacteriosis. Recent studies have reported that Campylobacter spp. are prone to developing resistance to antibiotics commonly used for their treatment, with many C. coli strains identified as multidrug-resistant. This study presents the results of the whole-genome sequencing analysis of the multidrug-resistant C. coli strain BCT3 isolated in Greece from a stool specimen of a pediatric patient presenting with diarrhea. The strain was isolated using selective culture media and, based on antimicrobial susceptibility tests, was found to be resistant to ciprofloxacin, tetracycline, erythromycin, azithromycin, clarithromycin, and doxycycline. To further characterize it, we performed whole-genome sequencing, which identified strain BCT3 as C. coli. Moreover, multilocus sequence typing assigned the BCT3 to the sequence type (ST) 872, belonging to clonal complex ST-828. The presence of multiple virulence genes revealed its pathogenic potential. The detection of antimicrobial resistance genes and mutated alleles was indicative of its resistance to fluoroquinolones, macrolides, and tetracyclines, supporting the observed phenotype. To our knowledge, this is the first reported clinical case of such a multidrug-resistant C. coli strain in Greece.<\/jats:p>","DOI":"10.3390\/microorganisms13061420","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T09:36:00Z","timestamp":1750239360000},"page":"1420","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome Analysis of the Multidrug-Resistant Campylobacter coli BCT3 of the Sequence Type (ST) 872 Isolated from a Pediatric Diarrhea Case"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9614-6543","authenticated-orcid":false,"given":"Konstantinos","family":"Papadimitriou","sequence":"first","affiliation":[{"name":"Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasios","family":"Ioannidis","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Microbiology, School of Medicine, Attikon University Hospital, National and Kapodistrian University of Athens, Rimini 1, 12462 Chaidari, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3104-4831","authenticated-orcid":false,"given":"Aleksandra","family":"Slavko","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, School of Agriculture and Food, University of the Peloponnese, 24100 Kalamata, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8419-8031","authenticated-orcid":false,"given":"Genovefa","family":"Chronopoulou","sequence":"additional","affiliation":[{"name":"Central Laboratory, Athens Medical Center, Distomou 5-7, 15125 Marousi, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nektarios","family":"Marmaras","sequence":"additional","affiliation":[{"name":"Central Laboratory, Athens Medical Center, Distomou 5-7, 15125 Marousi, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Pangalis","sequence":"additional","affiliation":[{"name":"Central Laboratory, Athens Medical Center, Distomou 5-7, 15125 Marousi, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisavet","family":"Olntasi","sequence":"additional","affiliation":[{"name":"Laboratory of Food Quality Control and Hygiene, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-3438","authenticated-orcid":false,"given":"Niki","family":"Vassilaki","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Department of Microbiology, Hellenic Pasteur Institute, Vasilissis Sofias 127, 11521 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Efthymia Ioanna","family":"Koufogeorgou","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Department of Microbiology, Hellenic Pasteur Institute, Vasilissis Sofias 127, 11521 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Kolida","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Department of Microbiology, Hellenic Pasteur Institute, Vasilissis Sofias 127, 11521 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4423-4906","authenticated-orcid":false,"given":"Dimitrios","family":"Theodoridis","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Athens Medical School, Aeginition Hospital, 11528 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1251-8355","authenticated-orcid":false,"given":"Stylianos","family":"Chatzipanagiotou","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Athens Medical School, Aeginition Hospital, 11528 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/CMR.00006-15","article-title":"Global epidemiology of Campylobacter infection","volume":"28","author":"Kaakoush","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.ebiom.2016.12.006","article-title":"Factors associated with sequelae of Campylobacter and non-typhoidal Salmonella infections: A systematic review","volume":"15","author":"Esan","year":"2017","journal-title":"EBioMedicine"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Sawicki, T., Ruszkowska, M., Danielewicz, A., Nied\u017awiedzka, E., Ar\u0142ukowicz, T., and Przyby\u0142owicz, K.E. (2021). A Review of colorectal cancer in terms of epidemiology, risk factors, development, symptoms and diagnosis. Cancers, 13.","DOI":"10.3390\/cancers13092025"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e1299","DOI":"10.1212\/WNL.0000000000200885","article-title":"An international perspective on preceding infections in Guillain-Barr\u00e9 syndrome: The IGOS-1000 cohort","volume":"99","author":"Leonhard","year":"2022","journal-title":"Neurology"},{"key":"ref_5","unstructured":"WHO (2024, November 21). Campylobacter. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/campylobacter."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Veronese, P., and Dodi, I. (2024). Campylobacter jejuni\/coli infection: Is it still a concern?. Microorganisms, 12.","DOI":"10.3390\/microorganisms12122669"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Quino, W., Caro-Castro, J., Hurtado, V., Flores-Le\u00f3n, D., Gonzalez-Escalona, N., and Gavilan, R.G. (2022). Genomic analysis and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli in Peru. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.802404"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1111\/zph.12611","article-title":"Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease: A review","volume":"66","author":"Rukambile","year":"2019","journal-title":"Zoonoses Public Health"},{"key":"ref_9","first-page":"75","article-title":"Last decade mini-review of the scientific progresses in the monitoring of the occurrence and antimicrobial susceptibility profile of poultry origin Campylobacter spp. within the European Union countries","volume":"32","author":"Popa","year":"2022","journal-title":"Rev. Rom. Med. Vet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1007\/s11259-024-10360-w","article-title":"First study in the frequency of isolation and phenotypic antimicrobial resistance profiles of pig and cattle origin Campylobacter strains in Romania","volume":"48","author":"Popa","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Popa, S.A., Herman, V., T\u00eerziu, E., Morar, A., Ban-Cucerzan, A., Imre, M., P\u0103tr\u00eenjan, R.-T., and Imre, K. (2025). Public health risk of Campylobacter spp. isolated from slaughterhouse and retail poultry meat: Prevalence and antimicrobial resistance profiles. Pathogens, 14.","DOI":"10.3390\/pathogens14040316"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1089\/fpd.2020.2795","article-title":"Prevalence of and risk factors for Campylobacter spp. colonization of broiler chicken flocks in Greece","volume":"17","author":"Natsos","year":"2020","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bunduru\u0219, I.A., Balta, I., \u0218tef, L., Ahmadi, M., Pe\u021b, I., McCleery, D., and Corcionivoschi, N. (2023). Overview of virulence and antibiotic resistance in Campylobacter spp. livestock isolates. Antibiotics, 12.","DOI":"10.3390\/antibiotics12020402"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Elmi, A., Nasher, F., Dorrell, N., Wren, B., and Gundogdu, O. (2021). Revisiting Campylobacter jejuni virulence and fitness factors: Role in sensing, adapting, and competing. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.607704"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wieczorek, K., Wo\u0142kowicz, T., and Osek, J. (2018). Antimicrobial resistance and virulence-associated traits of Campylobacter jejuni isolated from poultry food chain and humans with diarrhea. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01508"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ioannidou, V., Ioannidis, A., Magiorkinis, E., Bagos, P., Nicolaou, C., Legakis, N., and Chatzipanagiotou, S. (2013). Multilocus sequence typing (and phylogenetic analysis) of Campylobacter jejuni and Campylobacter coli strains isolated from clinical cases in Greece. BMC Res. Notes, 6.","DOI":"10.1186\/1756-0500-6-359"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Magana, M., Chatzipanagiotou, S., Burriel, A.R., and Ioannidis, A. (2017). Inquiring into the gaps of Campylobacter surveillance methods. Vet. Sci., 4.","DOI":"10.3390\/vetsci4030036"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetimm.2014.09.003","article-title":"Interaction between Campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host","volume":"162","author":"Aguilar","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_19","unstructured":"Ikejiofor, O.K., Tion, M.T., and Shima, F.K. (2024). Campylobacter: Virulence factors and pathogenesis. Recent Advances in Bacterial Biofilm Studies\u2014Formation, Regulation, and Eradication in Human Infections, IntechOpen."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kova\u010d, J., \u010cade\u017e, N., Stessl, B., Stingl, K., Gruntar, I., Ocepek, M., Trkov, M., Wagner, M., and Smole Mo\u017eina, S. (2015). High genetic similarity of ciprofloxacin-resistant Campylobacter jejuni in central Europe. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.01169"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, X., Zhou, Q., Tang, M., Pu, J., Zhang, J., Lu, J., Zhang, Y., and Gao, Y. (2021). Aminoglycoside resistance and possible mechanisms in Campylobacter spp. isolated from chicken and swine in Jiangsu, China. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.716185"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nunes, A., Oleastro, M., Alves, F., Liassine, N., Lowe, D.M., Benejat, L., Ducounau, A., Jehanne, Q., Borges, V., and Gomes, J.P. (2023). Recurrent Campylobacter jejuni infections with in vivo selection of resistance to macrolides and carbapenems: Molecular characterization of resistance determinants. Microbiol. Spectr., 11.","DOI":"10.1128\/spectrum.01070-23"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ghimpe\u021beanu, O.M., Pogurschi, E.N., Popa, D.C., Dragomir, N., Dr\u0103gotoiu, T., Mihai, O.D., and Petcu, C.D. (2022). Antibiotic use in livestock and residues in food\u2014A public health threat: A review. Foods, 11.","DOI":"10.3390\/foods11101430"},{"key":"ref_24","unstructured":"European Centre for Disease Prevention and Control (ECDC) (2024, October 24). Campylobacteriosis Annual Epidemiological Report for 2022. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/campylobacteriosis-annual-epidemiological-report-2022."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"EFSA, and European Centre for Disease Prevention and Control (ECDC) (2024). The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2021\u20132022. EFSA J., 22, e8583.","DOI":"10.2903\/j.efsa.2024.8583"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1086\/600885","article-title":"Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry","volume":"49","author":"Seng","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_27","unstructured":"EUCAST (2024, March 15). European Committee on Antimicrobial Susceptibility Testing Disk Diffusion Test Methodology. Available online: https:\/\/www.eucast.org\/ast_of_bacteria\/disk_diffusion_methodology."},{"key":"ref_28","unstructured":"EUCAST (2024, March 15). European Committee on Antimicrobial Susceptibility Testing, Clinical Breakpoints\u2014Breakpoints and Guidance. Version 14.0. Available online: https:\/\/www.eucast.org\/clinical_breakpoints."},{"key":"ref_29","unstructured":"Andrews, S. (2024, July 20). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"i302","DOI":"10.1093\/bioinformatics\/btu280","article-title":"Ragout\u2014A reference-assisted assembly tool for bacterial genomes","volume":"30","author":"Kolmogorov","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e323","DOI":"10.1002\/cpz1.323","article-title":"BUSCO: Assessing genomic data quality and beyond","volume":"1","author":"Manni","year":"2021","journal-title":"Curr. Protoc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2620","DOI":"10.1093\/bioinformatics\/btq484","article-title":"Circoletto: Visualizing sequence similarity with Circos","volume":"26","author":"Darzentas","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/bioinformatics\/btn578","article-title":"DNAPlotter: Circular and linear interactive genome visualization","volume":"25","author":"Carver","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"Carattoli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_39","unstructured":"Seemann, T. (2024, July 20). Snippy: Fast Bacterial Variant Calling from NGS Reads. Available online: https:\/\/github.com\/tseemann\/snippy."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1128\/JCM.39.1.14-23.2001","article-title":"Multilocus sequence typing system for Campylobacter jejuni","volume":"39","author":"Dingle","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1128\/JCM.43.5.2315-2329.2005","article-title":"Extended multilocus sequence typing system for Campylobacter coli, C. lari, C. upsaliensis, and C. helveticus","volume":"43","author":"Miller","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1128\/JCM.00080-17","article-title":"Core genome multilocus sequence typing scheme for stable, comparative analyses of Campylobacter jejuni and C. coli human disease isolates","volume":"55","author":"Cody","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications","volume":"3","author":"Jolley","year":"2018","journal-title":"Wellcome Open Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Aziz, R.K., Bartels, D., Best, A.A., DeJongh, M., Disz, T., Edwards, R.A., Formsma, K., Gerdes, S., Glass, E.M., and Kubal, M. (2008). The RAST Server: Rapid Annotations using Subsystems Technology. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-75"},{"key":"ref_45","unstructured":"Seemann, T. (2024, July 20). Abricate. Github. Available online: https:\/\/github.com\/tseemann\/abricate."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ortega-Sanz, I., Barbero-Aparicio, J.A., Canepa-Oneto, A., Rovira, J., and Melero, B. (2023). CamPype: An open-source workflow for automated bacterial whole-genome sequencing analysis focused on Campylobacter. BMC Bioinform., 24.","DOI":"10.1186\/s12859-023-05414-w"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"D912","DOI":"10.1093\/nar\/gkab1107","article-title":"VFDB 2022: A general classification scheme for bacterial virulence factors","volume":"50","author":"Liu","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Feldgarden, M., Brover, V., Gonzalez-Escalona, N., Frye, J.G., Haendiges, J., Haft, D.H., Hoffmann, M., Pettengill, J.B., Prasad, A.B., and Tillman, G.E. (2021). AMRFinderPlus and the reference gene catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-91456-0"},{"key":"ref_49","first-page":"000748","article-title":"ResFinder\u2014An open online resource for identification of antimicrobial resistance genes in next-generation sequencing data and prediction of phenotypes from genotypes","volume":"8","author":"Florensa","year":"2022","journal-title":"Microb. Genom."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"D566","DOI":"10.1093\/nar\/gkw1004","article-title":"CARD 2017: Expansion and model-centric curation of the comprehensive antibiotic resistance database","volume":"45","author":"Jia","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1128\/AAC.01310-13","article-title":"ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes","volume":"58","author":"Gupta","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"D561","DOI":"10.1093\/nar\/gkz1010","article-title":"MEGARes 2.0: A database for classification of antimicrobial drug, biocide and metal resistance determinants in metagenomic sequence data","volume":"48","author":"Doster","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Zarske, M., Luu, H.Q., Deneke, C., Kn\u00fcver, M.-T., Thieck, M., Hoang, H.T.T., Bretschneider, N., Pham, N.T., Huber, I., and Stingl, K. (2024). Identification of knowledge gaps in whole-genome sequence analysis of multi-resistant thermotolerant Campylobacter spp.. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10014-w"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1073\/pnas.95.6.3140","article-title":"Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic\u2009microorganisms","volume":"95","author":"Maiden","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"101703","DOI":"10.1016\/j.psj.2022.101703","article-title":"The prevalence, counts, and MLST genotypes of Campylobacter in poultry meat and genomic comparison with clinical isolates","volume":"101","author":"Tedersoo","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Tang, Y., Jiang, Q., Tang, H., Wang, Z., Yin, Y., Ren, F., Kong, L., Jiao, X., and Huang, J. (2020). Characterization and prevalence of Campylobacter spp. from broiler chicken rearing period to the slaughtering process in Eastern China. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00227"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/tbed.13046","article-title":"Molecular epidemiology and antimicrobial resistance mechanisms of Campylobacter coli from diarrhoeal patients and broiler carcasses in Belgium","volume":"66","author":"Elhadidy","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"103485","DOI":"10.1016\/j.psj.2024.103485","article-title":"Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing","volume":"103","author":"Phu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Audu, B.J., Norval, S., Bruno, L., Meenakshi, R., Marion, M., and Forbes, K.J. (2022). Genomic diversity and antimicrobial resistance of Campylobacter spp. from humans and livestock in Nigeria. J. Biomed. Sci., 29.","DOI":"10.1186\/s12929-022-00786-2"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e00797-23","DOI":"10.1128\/spectrum.00797-23","article-title":"Prevalence and genetic characterization of Campylobacter from clinical poultry cases in China","volume":"11","author":"Li","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Wang, L., Li, F., Liu, T., Ouyang, C., Wang, X., Li, M., Huang, Z., Huang, J., Wang, L., and Wang, X. (2024). Identification of a multidrug resistance genomic island harboring a nonfunctional optrA gene in Campylobacter coli of chicken origin. Vet. Microbiol., 293.","DOI":"10.1016\/j.vetmic.2024.110083"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Bai, Y., Ma, J., Li, F., Yang, B., Ren, X., Wang, Y., Hu, Y., Dong, Y., Wang, W., and Zhang, J. (2024). Antimicrobial resistance and genomic characterization of Campylobacter jejuni and Campylobacter coli isolated from retail chickens in Beijing, China. Microorganisms, 12.","DOI":"10.3390\/microorganisms12081601"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Zhang, P., Zhang, X., Liu, Y., Jiang, J., Shen, Z., Chen, Q., and Ma, X. (2020). Multilocus sequence types and antimicrobial resistance of Campylobacter jejuni and C. coli isolates of human patients from Beijing, China, 2017\u20132018. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.554784"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Mossong, J., Mughini-Gras, L., Penny, C., Devaux, A., Olinger, C., Losch, S., Cauchie, H.M., van Pelt, W., and Ragimbeau, C. (2016). Human Campylobacteriosis in Luxembourg, 2010\u20132013: A case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. Sci. Rep., 6.","DOI":"10.1038\/srep20939"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e02835-22","DOI":"10.1128\/mbio.02835-22","article-title":"Genomic insights into the increased occurrence of Campylobacteriosis caused by antimicrobial-resistant Campylobacter coli","volume":"13","author":"Zhang","year":"2022","journal-title":"mBio"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Gao, F., Tu, L., Chen, M., Chen, H., Zhang, X., Zhuang, Y., Luo, J., and Chen, M. (2023). Erythromycin resistance of clinical Campylobacter jejuni and Campylobacter coli in Shanghai, China. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1145581"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Kim, S.Y., An, D., Jeong, H., and Kim, J. (2024). Antimicrobial susceptibility patterns and genetic diversity of Campylobacter spp. isolates from patients with diarrhea in South Korea. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010094"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e01668-24","DOI":"10.1128\/aac.01668-24","article-title":"Increasing rates of erm(B) and erm(N) in human Campylobacter coli and Campylobacter jejuni erythromycin-resistant isolates between 2018 and 2023 in France","volume":"69","author":"Jehanne","year":"2025","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Sheppard, S.K., Dallas, J.F., Wilson, D.J., Strachan, N.J.C., McCarthy, N.D., Jolley, K.A., Colles, F.M., Rotariu, O., Ogden, I.D., and Forbes, K.J. (2010). Evolution of an agriculture-associated disease causing Campylobacter coli clade: Evidence from national surveillance data in Scotland. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015708"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1264\/jsme2.ME18115","article-title":"Phylogenetic diversity and antimicrobial resistance of Campylobacter coli from humans and animals in Japan","volume":"34","author":"Asakura","year":"2019","journal-title":"Microbes Environ."},{"key":"ref_72","first-page":"169","article-title":"Campylobacter virulence factors and molecular host\u2013pathogen interactions","volume":"431","author":"Tegtmeyer","year":"2021","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.tim.2007.09.006","article-title":"Campylobacter flagella: Not just for motility","volume":"15","author":"Guerry","year":"2007","journal-title":"Trends Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"10409","DOI":"10.1007\/s00253-020-10974-5","article-title":"Campylobacter sp.: Pathogenicity factors and prevention methods\u2014New molecular targets for innovative antivirulence drugs?","volume":"104","author":"Kreling","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.fm.2014.11.017","article-title":"Campylobacter virulence and survival factors","volume":"48","author":"Bolton","year":"2015","journal-title":"Food Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Konkel, M.E., Talukdar, P.K., Negretti, N.M., and Klappenbach, C.M. (2020). Taking control: Campylobacter jejuni binding to fibronectin sets the stage for cellular adherence and invasion. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00564"},{"key":"ref_77","first-page":"e77","article-title":"Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro","volume":"4","author":"Madrid","year":"2015","journal-title":"Emerg. Microbes Infect."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Radomska, K.A., W\u00f6sten, M.M.S.M., Ordo\u00f1ez, S.R., Wagenaar, J.A., and van Putten, J.P.M. (2017). Importance of Campylobacter jejuni FliS and FliW in flagella biogenesis and flagellin secretion. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.01060"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jgar.2023.05.009","article-title":"Genomic characterisation of Campylobacter jejuni Cj26: A high-level ciprofloxacin\/erythromycin-resistant strain isolated from a poultry carcass in southern Brazil","volume":"34","author":"Dias","year":"2023","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Karki, A.B., Khatri, B., and Fakhr, M.K. (2023). Transcriptome analysis of Campylobacter jejuni and Campylobacter coli during cold stress. Pathogens, 12.","DOI":"10.3390\/pathogens12070960"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Admasie, A., Wei, X., Johnson, B., Burns, L., Pawar, P., Aurand-Cravens, A., Voloshchuk, O., Dudley, E.G., Sisay Tessema, T., and Zewdu, A. (2024). Genomic diversity of Campylobacter jejuni and Campylobacter coli isolated from the Ethiopian dairy supply chain. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0305581"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0966-842X(02)02323-5","article-title":"Exploiting genome sequence: Predictions for mechanisms of Campylobacter chemotaxis","volume":"10","author":"Marchant","year":"2002","journal-title":"Trends Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1556\/EuJMI.2.2012.1.5","article-title":"Chemotaxis in Campylobacter jejuni","volume":"2","author":"Zautner","year":"2012","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"16214","DOI":"10.1074\/jbc.M400357200","article-title":"The FlgS\/FlgR Two-component signal transduction system regulates the fla Regulon in Campylobacter jejuni","volume":"279","author":"Wagenaar","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1007\/s00018-017-2696-5","article-title":"Flagellin glycosylation with pseudaminic acid in Campylobacter and Helicobacter: Prospects for development of novel therapeutics","volume":"75","author":"Roujeinikova","year":"2018","journal-title":"Cell Mol. Life Sci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1046\/j.1365-2958.1996.369895.x","article-title":"Identification and characterization of genes required for post-translational modification of Campylobacter coli VC167 flagellin","volume":"19","author":"Guerry","year":"1996","journal-title":"Mol. Microbiol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/j.1365-2958.2006.05100.x","article-title":"Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence","volume":"60","author":"Guerry","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1128\/JB.184.8.2100-2107.2002","article-title":"Characterization of the Campylobacter jejuni heptosyltransferase ii gene, waaf, provides genetic evidence that extracellular polysaccharide is lipid a core independent","volume":"184","author":"Oldfield","year":"2002","journal-title":"J. Bacteriol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1074\/jbc.M706163200","article-title":"Structure and function of sedoheptulose-7-phosphate isomerase, a critical enzyme for lipopolysaccharide biosynthesis and a target for antibiotic adjuvants","volume":"283","author":"Taylor","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0378-1119(98)00501-0","article-title":"Cloning, sequencing, and characterization of the lipopolysaccharide biosynthetic enzyme heptosyltransferase I gene (waaC) from Campylobacter jejuni and Campylobacter coli","volume":"222","author":"Klena","year":"1998","journal-title":"Gene"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1365-2958.2004.04374.x","article-title":"Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses","volume":"55","author":"Karlyshev","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1101\/gr.185801","article-title":"Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity","volume":"11","author":"Dorrell","year":"2001","journal-title":"Genome Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1128\/JCM.43.6.2771-2781.2005","article-title":"Comparison of Campylobacter jejuni lipooligosaccharide biosynthesis loci from a variety of sources","volume":"43","author":"Parker","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Poudel, S., Li, T., Chen, S., Zhang, X., Cheng, W.H., Sukumaran, A.T., Kiess, A.S., and Zhang, L. (2022). Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter isolated from broilers and broiler meat raised without antibiotics. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.00251-22"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"13425","DOI":"10.1038\/ncomms13425","article-title":"Complete structure of the bacterial flagellar hook reveals extensive set of stabilizing interactions","volume":"7","author":"Matsunami","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1128\/AEM.01507-07","article-title":"Identification of Campylobacter jejuni genes involved in the response to acidic pH and stomach transit","volume":"74","author":"Reid","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1099\/mic.0.000648","article-title":"The FlaG regulator is involved in length control of the polar flagella of Campylobacter jejuni","volume":"164","author":"Inoue","year":"2018","journal-title":"Microbiology"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Freitag, C.M., Strijbis, K., and van Putten, J.P.M. (2017). Host cell binding of the flagellar tip protein of Campylobacter jejuni. Cell Microbiol., 19.","DOI":"10.1111\/cmi.12714"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1046\/j.1365-2958.2003.03725.x","article-title":"Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene","volume":"50","author":"Goon","year":"2003","journal-title":"Mol. Microbiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.bbrc.2022.10.050","article-title":"Structural analysis of the pseudaminic acid synthase PseI from Campylobacter jejuni","volume":"635","author":"Song","year":"2022","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_101","unstructured":"The National Antimicrobial Resistance Monitoring System (2025, April 20). Global Resistome Data, Available online: https:\/\/www.fda.gov\/animal-veterinary\/national-antimicrobial-resistance-monitoring-system\/global-resistome-data."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1038\/ncomms2470","article-title":"Mechanism of tetracycline resistance by ribosomal protection protein Tet(O)","volume":"4","author":"Li","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Benites, C., Anampa, D., Torres, D., Avalos, I., Rojas, M., Conte, C., and L\u00e1zaro, C. (2022). Prevalence, tetracycline resistance and tet(O) gene identification in pathogenic Campylobacter strains isolated from chickens in retail markets of Lima, Peru. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111580"},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Casagrande Proietti, P., Guelfi, G., Bellucci, S., De Luca, S., Di Gregorio, S., Pieramati, C., and Franciosini, M.P. (2020). Beta-lactam resistance in Campylobacter coli and Campylobacter jejuni chicken isolates and the association between blaOXA-61 gene expression and the action of \u03b2-lactamase inhibitors. Vet. Microbiol., 241.","DOI":"10.1016\/j.vetmic.2019.108553"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jgar.2023.03.004","article-title":"Antimicrobial resistance genotypes and phenotypes of Campylobacter coli isolated from different sources over a 16-year period in Brazil","volume":"33","author":"Gomes","year":"2023","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Marotta, F., Janowicz, A., Romantini, R., Di Marcantonio, L., Di Timoteo, F., Romualdi, T., Zilli, K., Barco, L., D\u2019Incau, M., and Mangone, I. (2023). Genomic and antimicrobial surveillance of Campylobacter population in Italian poultry. Foods, 12.","DOI":"10.3390\/foods12152919"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Shen, Z., Wang, Y., Zhang, Q., and Shen, J. (2018). Antimicrobial resistance in Campylobacter spp.. Microbiol. Spectr., 6.","DOI":"10.1128\/microbiolspec.ARBA-0013-2017"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Cobo-D\u00edaz, J.F., Gonz\u00e1lez del R\u00edo, P., and \u00c1lvarez-Ord\u00f3\u00f1ez, A. (2021). Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.662144"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Ghatak, S., Milton, A.A.P., Das, S., Momin, K.M., Srinivas, K., Pyngrope, D.A., and Priya, G.B. (2024). Campylobacter coli of porcine origin exhibits an open pan-genome within a single clonal complex: Insights from comparative genomic analysis. Front. Cell. Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1449856"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Horme\u00f1o, L., Palomo, G., Borge, C., Vadillo, S., P\u00edriz, S., Dom\u00ednguez, L., Campos, M.J., and Quesada, A. (2018). ant(6)-I genes encoding aminoglycoside o-nucleotidyltransferases are widely spread among streptomycin resistant strains of Campylobacter jejuni and Campylobacter coli. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02515"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"e01543-16","DOI":"10.1128\/mBio.01543-16","article-title":"Emergence of a potent multidrug efflux pump variant that enhances Campylobacter resistance to multiple antibiotics","volume":"7","author":"Yao","year":"2016","journal-title":"mBio"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1574-6968.2007.00935.x","article-title":"Antibiotic resistance and resistance mechanisms in Campylobacter jejuni and Campylobacter coli","volume":"277","author":"Alfredson","year":"2007","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Portes, A.B., Panzenhagen, P., dos Santos, A.M.P., de Jesus, A.C.S., Ochioni, A.C., Duque, S.S., Aburjaile, F.F., Brenig, B., Azevedo, V., and Conte Junior, C.A. (2024). Draft genomes of multidrug-resistant Campylobacter jejuni and Campylobacter coli strains from Brazil representing novel sequence types. Microbiol. Resour. Announc., 13.","DOI":"10.1128\/mra.00524-24"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1128\/AAC.34.6.1294","article-title":"Emergence of aminoglycoside resistance genes aadA and aadE in the genus Campylobacter","volume":"34","author":"Mabilat","year":"1990","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.1128\/AAC.46.7.2124-2131.2002","article-title":"CmeABC functions as a multidrug efflux system in Campylobacter jejuni","volume":"46","author":"Lin","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"5378","DOI":"10.1021\/acs.chemrev.0c00621","article-title":"Structural and functional diversity of resistance-nodulation-cell division transporters","volume":"121","author":"Klenotic","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Bravo, V., Katz, A., Porte, L., Weitzel, T., Varela, C., Gonzalez-Escalona, N., and Blondel, C.J. (2021). Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile. PLoS Neglected Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009207"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Grinnage-Pulley, T., and Zhang, Q. (2015). Genetic basis and functional consequences of differential expression of the CmeABC efflux pump in Campylobacter jejuni Isolates. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0131534"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1128\/AAC.49.3.1067-1075.2005","article-title":"CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni","volume":"49","author":"Lin","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.trsl.2020.04.009","article-title":"New and alternative strategies for the prevention, control, and treatment of antibiotic-resistant Campylobacter","volume":"223","author":"Dai","year":"2020","journal-title":"Transl. Res."},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Du, Y., Wang, C., Ye, Y., Liu, Y., Wang, A., Li, Y., Zhou, X., Pan, H., Zhang, J., and Xu, X. (2018). Molecular identification of multidrug-resistant Campylobacter species from diarrheal patients and poultry meat in Shanghai, China. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01642"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/6\/1420\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:54:24Z","timestamp":1760032464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/6\/1420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,18]]},"references-count":121,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["microorganisms13061420"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13061420","relation":{},"ISSN":["2076-2607"],"issn-type":[{"type":"electronic","value":"2076-2607"}],"subject":[],"published":{"date-parts":[[2025,6,18]]}}}