{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:40:27Z","timestamp":1792194027765,"version":"4.4.0"},"reference-count":152,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T00:00:00Z","timestamp":1750464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The global consumption of fresh and ready-to-eat (RTE) fruits and vegetables has surged due to increasing awareness of their nutritional benefits. However, this trend has been accompanied by a rise in foodborne illness outbreaks linked to microbial contamination. This narrative review synthesizes current knowledge on the prevalence and diversity of foodborne pathogens in fresh produce, including bacterial, viral, and fungal agents. It critically evaluates both conventional and emerging detection methods, ranging from culture-based techniques and immunoassays to advanced molecular diagnostics, biosensors, flow cytometry (FC), and hyperspectral imaging (HSI). Additionally, this review discusses cutting-edge control strategies, such as natural antifungal agents, essential oils, biocontrol methods, and non-thermal technologies like cold plasma and UV-C treatment. Emphasis is placed on sampling methodologies, sustainability, One Health perspectives, and regulatory considerations. By highlighting recent technological advances and their limitations, this review aims to support the development of integrated, effective, and safe microbial control approaches for the fresh produce supply chain.<\/jats:p>","DOI":"10.3390\/microorganisms13071447","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T03:39:26Z","timestamp":1750649966000},"page":"1447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Emerging Technologies and Integrated Strategies for Microbial Detection and Control in Fresh Produce"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7726-9532","authenticated-orcid":false,"given":"Ayman","family":"Elbehiry","sequence":"first","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1699-6263","authenticated-orcid":false,"given":"Eman","family":"Marzouk","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feras","family":"Alzaben","sequence":"additional","affiliation":[{"name":"Department of Food Service, King Fahad Armed Hospital, Jeddah 23311, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Almuaither","sequence":"additional","affiliation":[{"name":"First Air Defense Group, Department of Nutrition, Riyadh 13624, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banan","family":"Abead","sequence":"additional","affiliation":[{"name":"Support Service Department, King Fahad Armed Hospital, Jeddah 23311, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Alamri","sequence":"additional","affiliation":[{"name":"Family & Community Medicine Department, Prince Sultan Medical Military City, Riyadh 12233, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9997-7476","authenticated-orcid":false,"given":"Abdulaziz M.","family":"Almuzaini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-3877","authenticated-orcid":false,"given":"Akram","family":"Abu-Okail","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"g4490","DOI":"10.1136\/bmj.g4490","article-title":"Fruit and vegetable consumption and mortality from all causes, cardiovascular disease, and cancer: Systematic review and dose-response meta-analysis of prospective cohort studies","volume":"349","author":"Wang","year":"2014","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1093\/ije\/dyw319","article-title":"Fruit and vegetable intake and the risk of cardiovascular disease, total cancer and all-cause mortality\u2014A systematic review and dose-response meta-analysis of prospective studies","volume":"46","author":"Aune","year":"2017","journal-title":"Int. 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