{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:14:43Z","timestamp":1787238883265,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>A total of 1.6 million tons of personal protective equipment (PPE) waste has been generated daily since 2019 and this production has not abated since that time. Within PPEs, isolation gowns make up the largest percentage by weight of landfill waste. This study aimed to evaluate the effectiveness of rapid, reproducible disinfection protocols to help facilitate safe reuse and minimize risks from microbial contamination. Disinfection of isolation gowns via fogging with hydrogen peroxide (HP) and hypochlorous acid (HC) were evaluated in the present study compared to standard ethylene oxide (EO) sterilization. This study was conducted at VCA West Coast Specialty and Emergency Animal Hospital in the United States. Ten isolation gowns (control) were cultured on tryptic soy agar contact plates in 10 predetermined areas to determine microbial load and morphology\/types on non-sterile gowns before use. Following this, 10 gowns were fogged with 12% HP, and then once drying was complete, they were cultured in the predetermined areas for microbial load and morphology\/types. This procedure was repeated with another set of 10 gowns fogged with 500 ppm HC. Lastly, 10 gowns were sterilized with EO using standard protocol and cultures were performed similarly. Median CFU (colony-forming unit) counts at 48 h for control, EO, HP, and HC were 4.5, 0, 0, and 0; at 72 h, they were 107, 0, 0, and 0, respectively. No significant difference was noted between the disinfection groups; post hoc pairwise analysis showed that the CFU counts for the disinfection groups were significantly lower than those for the control. The median percent reduction at 48 h for EO, HP, and HC was 100, 100, and 100; at 72 h, it was 100, 100, and 100, respectively. No significant difference was detected among the groups. The median number of microbe types for control, EO, HP, and HC was 2.5, 0, 0, and 0; there was no difference between the disinfection groups, but the number of microbe types was significantly higher for the control than for the disinfection groups. EO is environmentally toxic, expensive, and carcinogenic; it requires prolonged disinfection cycle times, expensive equipment, and trained personnel. This study suggests that HP and HC provide a cost-effective, relatively nontoxic, environmentally safe, and comparatively short disinfection time option for the disinfection and reuse of isolation gowns that does not require trained personnel or specialized equipment.<\/jats:p>","DOI":"10.3390\/microorganisms13071537","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T10:03:48Z","timestamp":1751277828000},"page":"1537","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fogging with Hydrogen Peroxide and Hypochlorous Acid: An Option for Disinfection and Reuse of Disposable Isolation Gowns in Medical Practice"],"prefix":"10.3390","volume":"13","author":[{"given":"Shay","family":"Iyer","sequence":"first","affiliation":[{"name":"VCA West Coast Specialty and Emergency Animal Hospital, 18300 Euclid Street, Fountain Valley, CA 92708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenhwa","family":"Ouyang","sequence":"additional","affiliation":[{"name":"1 IDEXX Drive, Westbrook, ME 04098, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arathi","family":"Vinayak","sequence":"additional","affiliation":[{"name":"VCA West Coast Specialty and Emergency Animal Hospital, 18300 Euclid Street, Fountain Valley, CA 92708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/S0195-6701(99)90065-8","article-title":"Infection Control: The Role of Disinfection and Sterilization","volume":"43","author":"Rutala","year":"1999","journal-title":"J. Hosp. Infect."},{"key":"ref_2","unstructured":"Rutala, W.A., and Weber, D.J. (2025, February 01). Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008 (Updated June 2024), Available online: https:\/\/www.cdc.gov\/infection-control\/hcp\/disinfection-and-sterilization\/index.html."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3912\/OJIN.Vol23No02PPT33","article-title":"Solid Waste and Disposal Processes for Isolated Patients with Infectious Disease","volume":"23","author":"Saber","year":"2018","journal-title":"Online J. Issues Nurs."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103663","DOI":"10.1016\/j.eti.2024.103663","article-title":"Engineering a Compostable Isolation Gown to Reduce Hospital-Derived Synthetic Waste Accumulation in Landfill","volume":"35","author":"Scott","year":"2024","journal-title":"Environ. Technol. Innov."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1097\/NNA.0000000000001160","article-title":"Analyzing the Cost of Hospital Contact Isolation Practices","volume":"52","author":"Saber","year":"2022","journal-title":"JONA J. Nurs. Adm."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Baker, N., Bromley-Dulfano, R., Chan, J., Gupta, A., Herman, L., Jain, N., Taylor, A.L., Lu, J., Pannu, J., and Patel, L. (2020). COVID-19 Solutions Are Climate Solutions: Lessons from Reusable Gowns. Front. Public. Health, 8.","DOI":"10.3389\/fpubh.2020.590275"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55811","DOI":"10.1007\/s11356-021-16025-4","article-title":"From Outbreak of COVID-19 to Launching of Vaccination Drive: Invigorating Single-Use Plastics, Mitigation Strategies, and Way Forward","volume":"28","author":"Das","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.marpolbul.2013.10.007","article-title":"Microplastic Pollution in the Surface Waters of the Laurentian Great Lakes","volume":"77","author":"Eriksen","year":"2013","journal-title":"Mar. Pollut. Bull."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Cubas, A.L.V., Moecke, E.H.S., Provin, A.P., Dutra, A.R.A., Machado, M.M., and Gouveia, I.C. (2023). The Impacts of Plastic Waste from Personal Protective Equipment Used during the COVID-19 Pandemic. Polymers, 15.","DOI":"10.20944\/preprints202305.1598.v1"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"119345","DOI":"10.1016\/j.envres.2024.119345","article-title":"Review on Personal Protective Equipment: Emerging Concerns in Micro(Nano)Plastic Pollution and Strategies for Addressing Environmental Challenges","volume":"257","author":"Le","year":"2024","journal-title":"Environ. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"160322","DOI":"10.1016\/j.scitotenv.2022.160322","article-title":"Personal Protective Equipment (PPE) Disposal during COVID-19: An Emerging Source of Microplastic and Microfiber Pollution in the Environment","volume":"860","author":"Khan","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"163984","DOI":"10.1016\/j.scitotenv.2023.163984","article-title":"Impact of Microfiber Pollution on Aquatic Biota: A Critical Analysis of Effects and Preventive Measures","volume":"887","author":"Priya","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jhazmat.2009.08.066","article-title":"Typical Pollutants in Bottom Ashes from a Typical Medical Waste Incinerator","volume":"173","author":"Zhao","year":"2010","journal-title":"J. Hazard. Mater."},{"key":"ref_14","unstructured":"(2024, August 01). Disinfectants for Emerging Viral Pathogens (EVPs): List Q, Available online: https:\/\/www.epa.gov\/pesticide-registration\/disinfectants-emerging-viral-pathogens-evps-list-q."},{"key":"ref_15","unstructured":"(2024, August 01). Chemical Disinfectants Guideline for Disinfection and Sterilization in Healthcare Facilities, Available online: https:\/\/www.cdc.gov\/infection-control\/hcp\/disinfection-sterilization\/chemical-disinfectants.html#toc."},{"key":"ref_16","first-page":"e79041","article-title":"Sterilization and Disinfection: Ensuring Infection Control in Dental Practices","volume":"17","year":"2025","journal-title":"Cureus"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s40201-024-00893-2","article-title":"Physicochemical Methods for Disinfection of Contaminated Surfaces\u2014A Way to Control Infectious Diseases","volume":"22","author":"Basak","year":"2024","journal-title":"J. Environ. Health Sci. Eng."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Destrez, P., and Beysens, D. (2025). Disinfection by Hydrogen Peroxide at Low Concentration in Air: The Key Role of Condensation. Anal. Biochem., 701.","DOI":"10.1016\/j.ab.2025.115786"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ayub, A., Cheong, Y.K., Castro, J.C., Cumberlege, O., and Chrysanthou, A. (2024). Use of Hydrogen Peroxide Vapour for Microbiological Disinfection in Hospital Environments: A Review. Bioengineering, 11.","DOI":"10.3390\/bioengineering11030205"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Walawska, A., Olak-Kucharczyk, M., Kaczmarek, A., and Kudzin, M.H. (2024). Environmentally Friendly Bleaching Process of the Cellulose Fibres Materials Using Ozone and Hydrogen Peroxide in the Gas Phase. Materials, 17.","DOI":"10.3390\/ma17061355"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1097\/00004630-199920060-00008","article-title":"THE 1999 LINDBERG AWARD 3% Hydrogen Peroxide for the Gram\u2013Positive Disinfection of Fabrics","volume":"20","author":"Neely","year":"1999","journal-title":"J. Burn Care Rehabil."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/j.ajic.2022.03.009","article-title":"Efficacy of Hypochlorous Acid (HOCl) Fog in Sanitizing Surfaces against Enterococcus Faecalis","volume":"50","author":"Feng","year":"2022","journal-title":"Am. J. Infect. Control"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4265\/jmc.28.4_165","article-title":"Uses of Gaseous Hypochlorous Acid for Controlling Microorganisms in Indoor Spaces","volume":"28","author":"Fukuzaki","year":"2023","journal-title":"J. Microorg. Control"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105","DOI":"10.4265\/jmc.29.3_105","article-title":"Volatilization and Disinfection Efficacy of Gaseous Hypochlorous Acid from an Air Washer-Type Humidifier in a Large Space","volume":"29","author":"Nojima","year":"2024","journal-title":"J. Microorg. Control"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1461","DOI":"10.1016\/j.joms.2020.06.029","article-title":"Hypochlorous Acid: A Review","volume":"78","author":"Block","year":"2020","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_26","unstructured":"Hinds, W.C. (1999). Properties, Behavior, and Measurement of Airborne Particles. Aerosol Technology, John Wiley & Sons."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"McDonnell, G.E. (2017). Antisepsis, Disinfection, and Sterilization: Types, Action, and Resistance, John Wiley & Sons.","DOI":"10.1128\/9781555819682"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, Z., Qu, Q., Wang, Z., Mou, S., Jiang, R., and Zhu, W. (2025). Association between Ethylene Oxide Exposure and Osteoarthritis Risk: An Analysis of NHANES Data (2013\u20132014 and 2017\u20132018). Front. Public Health, 13.","DOI":"10.3389\/fpubh.2025.1511215"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1265\/ehpm.24-00248","article-title":"Association of Ethylene Oxide Exposure and Obstructive Sleep Apnea","volume":"30","author":"Ma","year":"2025","journal-title":"Environ. Health Prev. Med."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, S., Wang, J., Lei, D., Peng, D., Zong, K., Li, K., Wu, Z., Liu, Y., and Huang, Z. (2024). Associations between Ethylene Oxide Exposure and Liver Function in the US Adult Population. Toxics, 12.","DOI":"10.3390\/toxics12080551"},{"key":"ref_31","first-page":"173","article-title":"Ethylene Oxide: Toxicology Review and Field Study Results of Hospital Use","volume":"2","author":"Glaser","year":"1979","journal-title":"J. Environ. Pathol. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1097\/00003226-200311000-00007","article-title":"Culture Plate Temperature and Delayed Incubation Effect on Bacterial Recovery","volume":"22","author":"Rudometkin","year":"2003","journal-title":"Cornea"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4265\/bio.22.1","article-title":"Ethylene Oxide Gas Sterilization of Medical Devices","volume":"22","author":"Shintani","year":"2017","journal-title":"Biocontrol Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1016\/j.ajic.2006.10.014","article-title":"Ethylene Oxide Sterilization of Medical Devices: A Review","volume":"35","author":"Mendes","year":"2007","journal-title":"Am. J. Infect. Control"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1586\/17434440.5.3.323","article-title":"Ethylene Oxide Potential Toxicity","volume":"5","year":"2008","journal-title":"Expert Rev. Med. Devices"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.ajic.2013.11.022","article-title":"Effectiveness of Improved Hydrogen Peroxide in Decontaminating Privacy Curtains Contaminated with Multidrug-Resistant Pathogens","volume":"42","author":"Rutala","year":"2014","journal-title":"Am. J. Infect. Control"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1365-2672.2011.05216.x","article-title":"Determining the Disinfection of Textiles in Compressed Carbon Dioxide Using Various Indicator Microbes","volume":"112","author":"Fijan","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jhin.2005.09.006","article-title":"Hydrogen Peroxide Fogging\/Fumigation","volume":"62","author":"McDonnell","year":"2006","journal-title":"J. Hosp. Infect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1177\/1535676020921007","article-title":"The Hitchhiker\u2019s Guide to Hydrogen Peroxide Fumigation, Part 1: Introduction to Hydrogen Peroxide Fumigation","volume":"25","author":"Albert","year":"2020","journal-title":"Appl. Biosaf."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Khandelwal, A., Lapolla, B., Bair, T., Grinstead, F., Hislop, M., Greene, C., and Bigham, M.T. (2022). Enhanced Disinfection with Hybrid Hydrogen Peroxide Fogging in a Critical Care Setting. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07704-9"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.cell.2008.09.024","article-title":"Bleach Activates a Redox-Regulated Chaperone by Oxidative Protein Unfolding","volume":"135","author":"Winter","year":"2008","journal-title":"Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4463","DOI":"10.1128\/AEM.02839-06","article-title":"Evaluation of Liquid- and Fog-Based Application of Sterilox Hypochlorous Acid Solution for Surface Inactivation of Human Norovirus","volume":"73","author":"Park","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Nasi\u0142owska, B., W\u0142odarski, M., Kaliszewski, M., Bogdanowicz, Z., Krzowski, \u0141., Kopczy\u0144ski, K., Witkowski, G., Czeczott-Urban, A., Bombalska, A., and Urba\u0144ska, M. (2024). Decontamination Effect of Hypochlorous Acid Dry Mist on Selected Bacteria, Viruses, Spores, and Fungi as Well as on Components of Electronic Systems. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25137198"},{"key":"ref_44","unstructured":"(2025, February 01). Medical Management Guidelines for Hydrogen Peroxide. Available online: https:\/\/www.webpoisoncontrol.org\/-\/media\/files\/pdf-for-article-dowloads-and-refs\/medical-management-guidelines-for-hydrogen-peroxide.pdf."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chen, F., Li, Y., Wang, W., Li, J., Wang, D., Sun, X., Peng, Y., and Deng, J. (2024). Comparative Performance of Contact Plate Metod and Swab Method for Surface Microbial Contamination on Medical Fabrics. BMC Infect. Dis., 24.","DOI":"10.1186\/s12879-024-09416-8"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jhin.2006.09.010","article-title":"An Evaluation of Different Methods for the Recovery of Meticillin-Resistant Staphylococcus Aureus from Environmental Surfaces","volume":"65","author":"Obee","year":"2007","journal-title":"J. Hosp. Infect."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1078\/S1438-4639(04)70035-8","article-title":"Comparison of Two Sampling Methods for the Detection of Gram-Positive and Gram-Negative Bacteria in the Environment: Moistened Swabs versus Rodac Plates","volume":"203","author":"Lemmen","year":"2001","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1128\/AAC.19.3.429","article-title":"Development and Validation of a Neutralizer System for in Vitro Evaluation of Some Antiseptics","volume":"19","author":"Sheikh","year":"1981","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/7\/1537\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:02:08Z","timestamp":1760032928000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/7\/1537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,30]]},"references-count":48,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["microorganisms13071537"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13071537","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,30]]}}}