{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:12:36Z","timestamp":1769159556388,"version":"3.49.0"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["721635"],"award-info":[{"award-number":["721635"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["01IT0720"],"award-info":[{"award-number":["01IT0720"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Special Research Fund BOF of Ghent University","award":["721635"],"award-info":[{"award-number":["721635"]}]},{"name":"Special Research Fund BOF of Ghent University","award":["01IT0720"],"award-info":[{"award-number":["01IT0720"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Colonization of plant tissues by bacterial endophytes might lead to qualitative and quantitative changes in secondary metabolites (SMs). In this work, in vitro co-culture experiments were performed using cell suspensions of the medicinal plant Alkanna tinctoria and eight of its bacterial endophytes. An untargeted metabolomics approach using Ultra-High-Performance Liquid Chromatography High-Resolution Mass Spectrometry (UHPLC-HRMS) was employed to investigate plant\u2013microbe interactions. Hierarchical clustering analysis and principal component analysis highlighted significant modifications of specific regulation patterns in SM production, caused by bacterial endophytes. The annotation step lead to the identification of 32 stimulated compounds in A. tinctoria cell suspensions. Among them, 3\u2032-hydroxy-14-hydroxyshikonofuran H (5), 8\u2032-decarboxy-rosmarinic acid (18), 8\u2034-decarboxy-salvianolic B (23), 8\u2033-8\u2034-didecarboxy-salvianolic acid B (26) were putatively identified for the first time. Our findings highlight that employing selected microbial inoculants under controlled conditions can be an effective strategy for enhancing or stimulating the production of specific high-value metabolites.<\/jats:p>","DOI":"10.3390\/microorganisms13071601","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T11:19:27Z","timestamp":1751887167000},"page":"1601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An UHPLC-HRMS-Based Untargeted Metabolomics Approach to Explore the Effects of Bacterial Endophyte Co-Culture on Alkanna tinctoria (L.) Tausch Cell Suspension Metabolome"],"prefix":"10.3390","volume":"13","author":[{"given":"Elodie","family":"Bossard","sequence":"first","affiliation":[{"name":"Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Cousy","sequence":"additional","affiliation":[{"name":"Pierre Fabre Dermo-Cosmetic & Personal Care (PFDC&PC), 31100 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Grondin","sequence":"additional","affiliation":[{"name":"Herbal Products Laboratory, Green Mission Department, Pierre Fabre Research Institute, 31035 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6829-9673","authenticated-orcid":false,"given":"Nikolaos","family":"Tsafantakis","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0781-4317","authenticated-orcid":false,"given":"Ang\u00e9lique","family":"Rat","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3335-8135","authenticated-orcid":false,"given":"Nektarios","family":"Aligiannis","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8421-2881","authenticated-orcid":false,"given":"Anne","family":"Willems","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laetitia","family":"Cattuzzato","sequence":"additional","affiliation":[{"name":"Pierre Fabre Dermo-Cosmetic & Personal Care (PFDC&PC), 31100 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thien","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Pierre Fabre Dermo-Cosmetic & Personal Care (PFDC&PC), 31100 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7028-1870","authenticated-orcid":false,"given":"Nikolas","family":"Fokialakis","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Taul\u00e9, C., Vaz-Jauri, P., and Battistoni, F. 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