{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:06:59Z","timestamp":1784603219843,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U21A201210"],"award-info":[{"award-number":["U21A201210"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The rumen and intestinal microbiota play a pivotal role in the digestion and absorption processes of ruminants. Elucidating the mechanisms by which gastrointestinal microbiota influence the feed conversion ratio (FCR) in ruminants is significantly important for enhancing feed utilization efficiency in these animals. In this study, RT-qPCR, 16S rRNA sequencing, and metabolomic techniques were systematically employed to compare the microbial community structures in the rumen, cecum, and rectum, as well as the differences in rumen metabolites between high- and low-FCR Tan sheep. The results showed that, compared to the HFCR group of Tan sheep, the LFCR group exhibited a significant reduction in unclassified_f__Selenomonadaceae, Blvii28_wastewater-sludge_group, and Papillibacter in the rumen; a significant increase in Lachnospiraceae_AC2044_group and Sanguibacteroides; a significant reduction in unclassified_f__Peptostreptococcaceae, Clostridium_sensu_stricto_1, and Parasutterella in the cecum; a significant increase in norank_f__Bacteroidales_UCG-001; and a significant reduction in norank_f__Muribaculaceae, Blautia, and Turicibacter in the rectum. There is a significant positive correlation between Parasutterella in the cecum and three microorganisms, including unclassified_f__Selenomonadaceae, in the rumen. Additionally, Blvii28_wastewater-sludge_group was positively correlated with Lactobacillus. Furthermore, unclassified_f__Selenomonadaceae in the rumen was positively correlated with Turicibacter, unclassified_f__Peptostreptococcaceae, and Breznakia in the rectum. Blvii28_wastewater-sludge_group also showed positive correlations with Blautia, norank_f__Muribaculaceae, and Clostridium_sensu_stricto_1, while Papillibacter was positively correlated with Faecalitalea. The metabolomic results indicated that, compared to the HFCR group, 261 differential metabolites, including Phenylacetylglutamine and Populin, in the rumen of Tan sheep in the LFCR group were significantly downregulated, whereas 36 differential metabolites, including Glycyl-L-tyrosine, were significantly upregulated. Furthermore, the rumen microbe unclassified_f__Selenomonadaceae exhibited positive correlations with significantly differential metabolites such as L-tryptophan, Etiocholanolone glucuronide, N-acetyl-O-demethylpuromycin, and 6-deoxyerythronolide B. Blvii28_wastewater-sludge_group and Papillibacter also exhibited positive correlations with Icilin. High and low FCRs in the rumen of Tan sheep were investigated, especially in relation to unclassified_f__Selenomonadaceae, Blvii28_wastewater-sludge_group, and Papillibacter. Correlations can be seen with microorganisms such as Parasutatella and Lactobacillus in the cecum; Turicibacter, norank_f__Bacteroideales_UCG-001, and Blautia in the rectum; and metabolites such as L-tryptophan, Etiocholanolone glucuronide, and N-acetyl-O-demethylpuromycin. This reveals the role of microorganisms in the digestion and absorption of Tan sheep feed, thus providing a preliminary basis for further research on the microbial regulation of ruminant animal feed utilization and a theoretical basis for improving Tan sheep feed utilization efficiency.<\/jats:p>","DOI":"10.3390\/microorganisms13071608","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T11:58:07Z","timestamp":1751975887000},"page":"1608","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Multi-Omics Integration Reveals the Impact of Gastrointestinal Microbiota on Feed Efficiency in Tan Sheep"],"prefix":"10.3390","volume":"13","author":[{"given":"Guohan","family":"Sun","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonggao","family":"Liu","sequence":"additional","affiliation":[{"name":"Animal Husbandry Workstation of Ningxia, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5296-160X","authenticated-orcid":false,"given":"An","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Yang","sequence":"additional","affiliation":[{"name":"Animal Husbandry Workstation of Ningxia, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8352-2805","authenticated-orcid":false,"given":"Jinzhong","family":"Tao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhao, X., Zuo, S., Guo, Y., Zhang, C., Wang, Y., Peng, S., Liu, M., Wang, B., Zhang, H., and Luo, H. (2023). Carcass Meat Quality, Volatile Compound Profile, and Gene Expression in Tan Sheep under Different Feeding Regimes. Food Biosci., 56.","DOI":"10.1016\/j.fbio.2023.103213"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102126","DOI":"10.1016\/j.parint.2020.102126","article-title":"Prevalence and Multilocus Genotyping of Giardia Duodenalis in Tan Sheep (Ovis Aries) in Northwestern China","volume":"77","author":"Peng","year":"2020","journal-title":"Parasitol. Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"148751","DOI":"10.1016\/j.gene.2024.148751","article-title":"Study on the Expression Patterns of Inner Root Sheath-Specific Genes in Tan Sheep Hair Follicle during Different Developmental Stages","volume":"927","author":"Shi","year":"2024","journal-title":"Gene"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"McLoughlin, S., Spillane, C., Claffey, N., Smith, P.E., O\u2019Rourke, T., Diskin, M.G., and Waters, S.M. (2020). Rumen Microbiome Composition Is Altered in Sheep Divergent in Feed Efficiency. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01981"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lu, M.-L., Pan, L., Zheng, C., Mao, R.-Y., Yuan, G.-H., Shi, C.-Y., Pu, Z.-H., Su, H.-X., Diao, Q.-Y., and Rehemujiang, H. (2025). Effects of Compound Microecological Preparation Supplementation on Production Performance and Nutrient Apparent Digestibility in Hu Sheep from the Rumen Perspective. Microorganisms, 13.","DOI":"10.3390\/microorganisms13050999"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.aninu.2024.04.003","article-title":"Dietary Crude Protein and Protein Solubility Manipulation Enhances Intestinal Nitrogen Absorption and Mitigates Reactive Nitrogen Emissions through Gut Microbiota and Metabolome Reprogramming in Sheep","volume":"18","author":"Zhang","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6122","DOI":"10.3168\/jds.2018-14632","article-title":"Milk Fat Depression in Dairy Ewes Fed Fish Oil: Might Differences in Rumen Biohydrogenation, Fermentation, or Bacterial Community Explain the Individual Variation?","volume":"101","author":"Frutos","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Huang, X., Zhou, L., You, X., Han, H., Chen, X., and Huang, X. (2023). Production Performance and Rumen Bacterial Community Structure of Hu Sheep Fed Fermented Spent Mushroom Substrate from Pleurotus Eryngii. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-35828-8"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.cell.2016.05.041","article-title":"From Dietary Fiber to Host Physiology: Short-Chain Fatty Acids as Key Bacterial Metabolites","volume":"165","author":"Koh","year":"2016","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e34380","DOI":"10.1016\/j.heliyon.2024.e34380","article-title":"The Characteristics of Intestinal Flora of Tibetan Sheep in Different Regions at High Altitude Were Revealed Based on Metagenomic Technique","volume":"10","author":"Gong","year":"2024","journal-title":"Heliyon"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107107","DOI":"10.1016\/j.smallrumres.2023.107107","article-title":"Does Intestinal Bacteria Diversity Drive Multi-Dimensional Sheep (Ovis Aries) Health?","volume":"228","author":"Kono","year":"2023","journal-title":"Small Rumin. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.anifeedsci.2019.05.003","article-title":"Performance, Rumen Fermentation, and Gastrointestinal Microflora of Lambs Fed Pelleted or Unpelleted Total Mixed Ration","volume":"253","author":"Zhang","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"109642","DOI":"10.1016\/j.meatsci.2024.109642","article-title":"Effects of Feeding Patterns on Production Performance, Lipo-Nutritional Quality and Gut Microbiota of Sunit Sheep","volume":"218","author":"Kang","year":"2024","journal-title":"Meat Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, Z., Shen, J., Xu, Y., and Zhu, W. (2020). Metagenomic Analysis Reveals Significant Differences in Microbiome and Metabolic Profiles in the Rumen of Sheep Fed Low N Diet with Increased Urea Supplementation. FEMS Microbiol. Ecol., 96.","DOI":"10.1093\/femsec\/fiaa117"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"100368","DOI":"10.1016\/j.vas.2024.100368","article-title":"Effects of Probiotic-Supplemented Milk Replacer on Growth, Blood Biochemistry, Fermentation, Digestibility, and Carcass Traits in Lambs","volume":"25","author":"Esfiokhi","year":"2024","journal-title":"Vet. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1016\/S2095-3119(21)63763-2","article-title":"Long Term Effects of Artificial Rearing before Weaning on the Growth Performance, Ruminal Microbiota and Fermentation of Fattening Lambs","volume":"21","author":"Huang","year":"2022","journal-title":"J. Integr. Agric."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Mohapatra, A., Trivedi, S., Tejpal, C.S., Aware, M.J., Vaswani, S., Prajapati, V.J., Kolte, A.P., Malik, P.K., Sahoo, A., and Ravishankar, C.N. (2025). Effect of Two Selected Levels of Padina Gymnospora Biowaste and Enteric Methane Emission, Nutrient Digestibility, and Rumen Metagenome in Growing Sheep. Microorganisms, 13.","DOI":"10.3390\/microorganisms13040780"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e05683","DOI":"10.1016\/j.heliyon.2020.e05683","article-title":"Effect of Dietary Inclusion of Graded Levels of Distillers Dried Grains with Solubles on the Performance, Blood Profile and Rumen Microbiota of Najdi Lambs","volume":"7","author":"Abudabos","year":"2021","journal-title":"Heliyon"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1016\/j.jia.2022.10.002","article-title":"Effect of Dioscorea Opposite Waste on Growth Performance, Blood Parameters, Rumen Fermentation and Rumen Bacterial Community in Weaned Lambs","volume":"22","author":"Guo","year":"2023","journal-title":"J. Integr. Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106290","DOI":"10.1016\/j.micpath.2023.106290","article-title":"Emerging Trends and Applications in Health-Boosting Microorganisms-Specific Strains for Enhancing Animal Health","volume":"183","author":"Fu","year":"2023","journal-title":"Microb. Pathog."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9209","DOI":"10.3168\/jds.2023-24479","article-title":"Influence of Empirically Derived Filtering Parameters, Amplicon Sequence Variant, and Operational Taxonomic Unit Pipelines on Assessing Rumen Microbial Diversity","volume":"107","author":"Tom","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2019.05.001","article-title":"Comparison of the Rumen Bacterial Community, Rumen Fermentation and Growth Performance of Fattening Lambs Fed Low-Grain, Pelleted or Non-Pelleted High Grain Total Mixed Ration","volume":"253","author":"Seddik","year":"2019","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"115797","DOI":"10.1016\/j.anifeedsci.2023.115797","article-title":"Alfalfa Intervention Alters the Colonization of Rumen Epithelial Bacteria to Promote Rumen Development and Lamb Health during Early Life","volume":"306","author":"Yang","year":"2023","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116269","DOI":"10.1016\/j.anifeedsci.2025.116269","article-title":"The Impact of Probiotic Live Yeast in a Barley Grain-Based Diet on Rumen Microbial Communities, Fermentation, and Histology of Artificially Reared Lambs","volume":"322","author":"Mavrommatis","year":"2025","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Grabner, E., Stare, E., Fanedl, L., Zorec, M., Jones, D.S., Johnston, C.D., Avgu\u0161tin, G., and Accetto, T. (2023). Expanding the Rumen Prevotella Collection: The Description of Prevotella Communis, Sp. Nov. of Ovine Origin. Syst. Appl. Microbiol., 46.","DOI":"10.1016\/j.syapm.2023.126437"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Gao, Q., Li, K., Zhong, R., Long, C., Liu, L., Chen, L., and Zhang, H. (2021). Supplementing Glycerol to Inoculum Induces Changes in pH, SCFA Profiles, and Microbiota Composition in In-Vitro Batch Fermentation. Fermentation, 8.","DOI":"10.3390\/fermentation8010018"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"113532","DOI":"10.1016\/j.jenvman.2021.113532","article-title":"Influence of Cosubstrate and Hydraulic Retention Time on the Removal of Drugs and Hygiene Products in Sanitary Sewage in an Anaerobic Expanded Granular Sludge Bed Reactor","volume":"299","author":"Granatto","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.nbt.2015.07.007","article-title":"Fluxes in PHA-Storing Microbial Communities during Enrichment and Biopolymer Accumulation Processes","volume":"33","author":"Janarthanan","year":"2016","journal-title":"New Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.anaerobe.2013.08.003","article-title":"Impact of Subacute Ruminal Acidosis (SARA) Adaptation on Rumen Microbiota in Dairy Cattle Using Pyrosequencing","volume":"24","author":"Mao","year":"2013","journal-title":"Anaerobe"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4984","DOI":"10.2527\/jas.2013-6304","article-title":"Alteration of Digestive Tract Microbiome in Neonatal Holstein Bull Calves by Bacitracin Methylene Disalicylate Treatment and Scours","volume":"91","author":"Xie","year":"2013","journal-title":"J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Huang, J., Sheng, Y., Jia, X., Qian, W., and Li, Z. (2025). Comparative Analysis of Microbial\u2013Short-Chain Fatty Acids\u2013Epithelial Transport Axis in the Rumen Ecosystem Between Tarim Wapiti (Cervus Elaphus Yarkandensis) and Karakul Sheep (Ovis Aries). Microorganisms, 13.","DOI":"10.3390\/microorganisms13051111"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"104900","DOI":"10.1016\/j.micpath.2021.104900","article-title":"Microbiome Analysis Reveals the Alterations in Gut Microbiota in Different Intestinal Segments of Yimeng Black Goats","volume":"155","author":"Li","year":"2021","journal-title":"Microb. Pathog."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100","DOI":"10.5713\/ajas.16.0166","article-title":"Characterization of the Microbial Communities along the Gastrointestinal Tract of Sheep by 454 Pyrosequencing Analysis","volume":"30","author":"Wang","year":"2016","journal-title":"Asian-Australas J. Anim. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zhang, B., Wang, X., Ding, Z., Kang, Y., Guo, S., Cao, M., Hu, L., Xiong, L., Pei, J., and Guo, X. (2024). Effects of High-Concentrate Diets on Growth Performance, Serum Biochemical Indexes, and Rumen Microbiota in House-Fed Yaks. Animals, 14.","DOI":"10.3390\/ani14243594"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100076","DOI":"10.1016\/j.animal.2020.100076","article-title":"Rumen Fluid Transplantation Affects Growth Performance of Weaned Lambs by Altering Gastrointestinal Microbiota, Immune Function and Feed Digestibility","volume":"15","author":"Yin","year":"2021","journal-title":"Animal"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tian, X.-Z., Li, J.-X., Luo, Q.-Y., Zhou, D., Long, Q.-M., Wang, X., Lu, Q., and Wen, G.-L. (2021). Effects of Purple Corn Anthocyanin on Blood Biochemical Indexes, Ruminal Fluid Fermentation, and Rumen Microbiota in Goats. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.715710"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"79","DOI":"10.5958\/0974-181X.2022.00007.5","article-title":"Effect of Feeding Slow Release Non Protein Nitrogen Sources on Nutrient Utilization, Rumen Fermentation Pattern, Microbial Protein Supply and Bacterial Diversity in Deccani Rams","volume":"22","author":"Manju","year":"2022","journal-title":"Anim. Nutr. Feed Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"102316","DOI":"10.1016\/j.anaerobe.2020.102316","article-title":"Rumen Bacterial Diversity in Relation to Nitrogen Retention in Beef Cattle","volume":"67","author":"Messana","year":"2021","journal-title":"Anaerobe"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Lei, W., Qi, M., Tan, P., Yang, S., Fan, L., Li, H., and Gao, Z. (2024). Impact of Polyphenol-Loaded Edible Starch Nanomaterials on Antioxidant Capacity and Gut Microbiota. Int. J. Biol. Macromol., 265.","DOI":"10.1016\/j.ijbiomac.2024.130979"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2502","DOI":"10.1002\/jsfa.13150","article-title":"Investigation the Nutritional Properties of Auricularia Auricula Pulp Fermented with Lactobacillus Strains and Their Effects on Gut Microbiota","volume":"104","author":"Li","year":"2024","journal-title":"J. Sci. Food Agric."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Bush, J.R., and Alfa, M.J. (2020). Increasing Levels of Parasutterella in the Gut Microbiome Correlate with Improving Low-Density Lipoprotein Levels in Healthy Adults Consuming Resistant Potato Starch during a Randomised Trial. BMC Nutr., 6.","DOI":"10.1186\/s40795-020-00398-9"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"101102","DOI":"10.1016\/j.animal.2024.101102","article-title":"Predicting Feed Efficiency of Angus Steers Using the Gastrointestinal Microbiome","volume":"18","author":"Congiu","year":"2024","journal-title":"animal"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"100216","DOI":"10.1016\/j.animal.2021.100216","article-title":"Garlic Skin Induces Shifts in the Rumen Microbiome and Metabolome of Fattening Lambs","volume":"15","author":"Zhu","year":"2021","journal-title":"Animal"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1203\/00006450-199102000-00009","article-title":"Phenylacetylglutamine May Replace Urea as a Vehicle for Waste Nitrogen Excretion","volume":"29","author":"Brusilow","year":"1991","journal-title":"Pediatr. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"107558","DOI":"10.1016\/j.ymgme.2023.107558","article-title":"Taste-Masked Formulation of Sodium Phenylbutyrate (ACER-001) for the Treatment of Urea Cycle Disorders","volume":"138","author":"Cederbaum","year":"2023","journal-title":"Mol. Genet. Metab."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e1531","DOI":"10.1210\/clinem\/dgae525","article-title":"Gut-Microbiota-Related Metabolite Phenylacetylglutamine and Risk of Incident Coronary Heart Disease Among Women","volume":"110","author":"Heianza","year":"2025","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ma, L., Fu, G., Liu, R., Zhou, F., Dong, S., Zhou, Y., Lou, J., and Wang, X. (2023). Phenylacetyl Glutamine: A Novel Biomarker for Stroke Recurrence Warning. BMC Neurol., 23.","DOI":"10.1186\/s12883-023-03118-5"},{"key":"ref_48","first-page":"101808","article-title":"Effects of the Dietary Protein Level on Growth Performance, Nitrogen Metabolism, Serum Biochemical Index, and Meat Quality of Suffolk\u00d7Hu F1 Lambs","volume":"21","author":"Li","year":"2025","journal-title":"J. Agric. Food Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"116257","DOI":"10.1016\/j.anifeedsci.2025.116257","article-title":"Probiotic-Inoculated Biochar as a Feed Additive for Dairy Sheep: Effect on Apparent Digestibility, Microbial Protein Supply, Methane Emissions and Productive Performance","volume":"321","author":"Benhissi","year":"2025","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.aninu.2023.02.009","article-title":"Alterations in Nutrient Digestion and Utilization Associated with Different Residual Feed Intake in Hu Sheep","volume":"13","author":"Zeng","year":"2023","journal-title":"Anim. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.1038\/s41564-024-01737-3","article-title":"Dietary Fibre Directs Microbial Tryptophan Metabolism via Metabolic Interactions in the Gut Microbiota","volume":"9","author":"Sinha","year":"2024","journal-title":"Nat. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"116287","DOI":"10.1016\/j.anifeedsci.2025.116287","article-title":"Effects of Sucralose Supplementation on Rumen Bacterial Community, Kynuronine Pathway and Feeding Behavior in Small-Tailed Han Sheep","volume":"323","author":"Hu","year":"2025","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"109267","DOI":"10.1016\/j.meatsci.2023.109267","article-title":"Tryptophan Improves Antioxidant Capability and Meat Quality by Reducing Responses to Stress in Nervous Hu Sheep","volume":"204","author":"Wang","year":"2023","journal-title":"Meat Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"115877","DOI":"10.1016\/j.anifeedsci.2024.115877","article-title":"Dietary Selenium Levels Affect Mineral Absorbability, Rumen Fermentation, Microbial Composition and Metabolites of the Grazing Sheep","volume":"308","author":"Meng","year":"2024","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1007\/s00726-016-2245-9","article-title":"Catabolism and Safety of Supplemental L-Arginine in Animals","volume":"48","author":"Wu","year":"2016","journal-title":"Amino Acids"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Robinson, M., Turnbull, S., Lee, B.Y., and Leonenko, Z. (2020). The Effects of Melatonin, Serotonin, Tryptophan and NAS on the Biophysical Properties of DPPC Monolayers. Biochim. Biophys. Acta (BBA) Biomembr., 1862.","DOI":"10.1016\/j.bbamem.2020.183363"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"115398","DOI":"10.1016\/j.anifeedsci.2022.115398","article-title":"Inclusion of the Aerial Part and Condensed Tannin Extract from Cistus Ladanifer L. in Lamb Diets\u2013Effects on Rumen Microbial Community and Fatty Acid Profile","volume":"291","author":"Guerreiro","year":"2022","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hassan, F., Guo, Y., Li, M., Tang, Z., Peng, L., Liang, X., and Yang, C. (2021). Effect of Methionine Supplementation on Rumen Microbiota, Fermentation, and Amino Acid Metabolism in In Vitro Cultures Containing Nitrate. Microorganisms, 9.","DOI":"10.3390\/microorganisms9081717"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Rocchetti, G., Becchi, P.P., Salis, L., Lucini, L., and Cabiddu, A. (2022). Impact of Pasture-Based Diets on the Untargeted Metabolomics Profile of Sarda Sheep Milk. Foods, 12.","DOI":"10.3390\/foods12010143"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/7\/1608\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:06:43Z","timestamp":1760033203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/7\/1608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,8]]},"references-count":59,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["microorganisms13071608"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13071608","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,8]]}}}