{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:43:58Z","timestamp":1783529038178,"version":"3.55.0"},"reference-count":45,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Papillomaviruses (PVs) are double-stranded DNA viruses known to induce a variety of epithelial lesions in dogs, ranging from benign hyperplasia to malignancies. In regions of rich biodiversity such as the Western Amazon, data on the circulation and genetic composition of canine papillomaviruses (CPVs) remain scarce. This study investigated CPV types present in oral and cutaneous papillomatous lesions in domiciled dogs from Acre and Rond\u00f4nia States, Brazil. Sixty-one dogs with macroscopically consistent lesions were clinically evaluated, and tissue samples were collected for histopathological examination and PCR targeting the L1 gene. Among these, 37% were histologically diagnosed as squamous papillomas or fibropapillomas, and 49.2% (30\/61) tested positive for papillomavirus DNA. Sequencing of the L1 gene revealed that most positive samples belonged to CPV1 (Lambdapapillomavirus 2), while one case was identified as CPV8 (Chipapillomavirus 3). Complete genomes of three CPV1 strains were obtained via high-throughput sequencing and showed high identity with CPV1 strains from other Brazilian regions. Phylogenetic analysis confirmed close genetic relationships among isolates across distinct geographic areas. These findings demonstrate the circulation of genetically conserved CPVs in the Amazon and reinforce the value of molecular and histopathological approaches for the accurate diagnosis and surveillance of viral diseases in domestic dogs, especially in ecologically complex regions.<\/jats:p>","DOI":"10.3390\/microorganisms13081811","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T09:41:17Z","timestamp":1754300477000},"page":"1811","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unveiling the Genetic Landscape of Canine Papillomavirus in the Brazilian Amazon"],"prefix":"10.3390","volume":"13","author":[{"given":"Jeneffer Caroline de Mac\u00eado","family":"Sousa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1987-8190","authenticated-orcid":false,"given":"Andr\u00e9 de Medeiros Costa","family":"Lins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Medicina Veterin\u00e1ria Preventiva e Vacinologia, Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1, Castanhal 68740-970, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda dos Anjos","family":"Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Higor Ortiz","family":"Manoel","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9273-7322","authenticated-orcid":false,"given":"Cleyton","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena Yanet C\u00e1ceres","family":"Tomaya","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Exatas e Tecnol\u00f3gicas, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7771-4106","authenticated-orcid":false,"given":"Paulo Henrique Gilio","family":"Gasparotto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4833-4400","authenticated-orcid":false,"given":"Vyctoria Malayhka de Abreu G\u00f3es","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo 93525-075, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ac\u00e1cio Duarte","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5804-7045","authenticated-orcid":false,"given":"Fernando Rosado","family":"Spilki","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo 93525-075, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1830-9017","authenticated-orcid":false,"given":"Mariana Soares da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale, Novo Hamburgo 93525-075, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7711-6437","authenticated-orcid":false,"given":"Felipe Masiero","family":"Salvarani","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Medicina Veterin\u00e1ria Preventiva e Vacinologia, Instituto de Medicina Veterin\u00e1ria, Universidade Federal do Par\u00e1, Castanhal 68740-970, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0621-243X","authenticated-orcid":false,"given":"Cl\u00e1udio Wageck","family":"Canal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul, Av. Bento Gon\u00e7alves, 9090, Pr\u00e9dio 42.505, Porto Alegre 91540-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1345-5413","authenticated-orcid":false,"given":"Flavio Roberto Chaves da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0795-3934","authenticated-orcid":false,"given":"C\u00edntia","family":"Daudt","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia Geral e Parasitologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Natureza, Universidade Federal do Acre, Campus Universit\u00e1rio, BR 364, Km 04-Distrito Industrial, Rio Branco 69920-900, AC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.virol.2004.03.033","article-title":"Classification of papillomaviruses","volume":"324","author":"Fauquet","year":"2004","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.virol.2010.02.002","article-title":"Classification of Papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments","volume":"401","author":"Bernard","year":"2010","journal-title":"Virology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1128\/JCM.02047-10","article-title":"Clinically healthy skin of dogs is a potential reservoir for canine papillomaviruses","volume":"49","author":"Lange","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"105897","DOI":"10.1016\/j.tvjl.2022.105897","article-title":"Papillomaviral skin diseases of humans, dogs, cats and horses: A comparative review. Part 1: Papillomavirus biology and hyperplastic lesions","volume":"288","author":"Munday","year":"2022","journal-title":"Vet. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105898","DOI":"10.1016\/j.tvjl.2022.105898","article-title":"Papillomaviral skin diseases of humans, dogs, cats and horses: A comparative review. Part 2: Pre-neoplastic and neoplastic diseases","volume":"288","author":"Munday","year":"2022","journal-title":"Vet. J."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Munday, J.S., Bond, S.D., Piripi, S., Soulsby, S.J., and Knox, M.A. (2024). Canis familiaris Papillomavirus Type 26: A Novel Papillomavirus of Dogs and the First Canine Papillomavirus within the Omegapapillomavirus Genus. Viruses, 16.","DOI":"10.3390\/v16040595"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1007\/s42770-020-00310-4","article-title":"Canine papillomavirus type 16 associated to squamous cell carcinoma in a dog: Virological and pathological findings","volume":"51","author":"Alves","year":"2020","journal-title":"Braz. J. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Munday, J.S., Gedye, K., Knox, M.A., Ravens, P., and Lin, X. (2022). Genomic Characterization of Canis familiaris Papillomavirus Type 24, a Novel Papillomavirus Associated with Extensive Pigmented Plaque Formation in a Pug Dog. Viruses, 14.","DOI":"10.3390\/v14112357"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1177\/03009858231195762","article-title":"Clinical, histopathological, and molecular characterization of canine pigmented viral plaques","volume":"60","author":"Orlandi","year":"2023","journal-title":"Vet. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1177\/0300985816630795","article-title":"Two Canine Papillomaviruses Associated with Metastatic Squamous Cell Carcinoma in Two Related Basenji Dogs","volume":"53","author":"Luff","year":"2016","journal-title":"Vet. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Chang, C.-Y., Chen, W.-T., Haga, T., Yamashita, N., Lee, C.-F., Tsuzuki, M., and Chang, H.-W. (2020). The detection and association of canine papillomavirus with benign and malignant skin lesions in dogs. Viruses, 12.","DOI":"10.3390\/v12020170"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-3164.2010.00913.x","article-title":"Development of multiple pigmented viral plaques and squamous cell carcinomas in a dog infected by a novel papillomavirus","volume":"22","author":"Munday","year":"2011","journal-title":"Vet. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1099\/0022-1317-80-9-2437","article-title":"A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin","volume":"80","author":"Forslund","year":"1999","journal-title":"J. Gen. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Daudt, C., da Silva, F., Streck, A.F., Weber, M.N., Cibulski, S.P., Mayer, F.Q., and Canal, C.W. (2016). How many papillomavirus species can go undetected in papilloma lesions?. Sci. Rep., 6.","DOI":"10.1038\/srep36480"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1007\/s11262-016-1406-y","article-title":"Genome characterization of a bovine papillomavirus type 5 from cattle in the Amazon region, Brazil","volume":"53","author":"Daudt","year":"2017","journal-title":"Virus Genes"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"vev003","DOI":"10.1093\/ve\/vev003","article-title":"RDP4: Detection and analysis of recombination patterns in virus genomes","volume":"1","author":"Martin","year":"2015","journal-title":"Virus Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies","volume":"32","author":"Nguyen","year":"2015","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"395","DOI":"10.2307\/2982890","article-title":"Fisher\u2019s Exact Test","volume":"155","author":"Upton","year":"1992","journal-title":"J. Roy. Statist. Soc. Ser. A"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1097\/00004032-198506000-00001","article-title":"A Monte Carlo primer for health physicists","volume":"48","author":"Turner","year":"1985","journal-title":"Health Phys."},{"key":"ref_21","unstructured":"R Core Team (2025). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.tvjl.2017.04.018","article-title":"Papillomaviruses in dogs and cats","volume":"225","author":"Munday","year":"2017","journal-title":"Vet. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.aimed.2020.12.003","article-title":"A topical botanical therapy for the treatment of canine papilloma virus associated oral warts: A case series","volume":"8","author":"Williams","year":"2021","journal-title":"Adv. Integr. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Schaefer, E.A.F., Chu, S., Pearce, J.W., Bryan, J.N., and Flesner, B.K. (2019). Papillomavirus DNA Not Detected in Canine Lobular Orbital Adenoma and Normal Conjunctival Tissue. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-1971-0"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0042-6822(88)90617-4","article-title":"\u201cHit and Run\u201d Transformation of Mouse C127 Cells by Bovine Papillomavirus Type 4: The Viral DNA Is Required for the Initiation but Not for Maintenance of the Transformed Phenotype","volume":"164","author":"Smith","year":"1988","journal-title":"Virology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1354\/vp.46-1-34","article-title":"Detection of Canine Oral Papillomavirus DNA in Conjunctival Epithelial Hyperplastic Lesions of Three Dogs","volume":"46","author":"Brandes","year":"2009","journal-title":"Vet. Pathol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1099\/vir.0.014498-0","article-title":"Three Novel Canine Papillomaviruses Support Taxonomic Clade Formation","volume":"90","author":"Lange","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"509-e141","DOI":"10.1111\/vde.12999","article-title":"Canine Pedal Papilloma Identification and Management: A Retrospective Series of 44 Cases","volume":"32","author":"Gould","year":"2021","journal-title":"Vet. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1177\/1040638712443360","article-title":"Detection of Six Novel Papillomavirus Sequences within Canine Pigmented Plaques","volume":"24","author":"Luff","year":"2012","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1590\/S0100-736X2014001200013","article-title":"Identification of Canine Papillomavirus Type 1 (CPV1) DNA in Dogs with Cutaneous Papillomatosis","volume":"34","author":"Alfieri","year":"2014","journal-title":"Pesqui. Vet. Bras."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/vde.12776","article-title":"Molecular Characterization of Canis Familiaris Oral Papillomavirus 1 Identified in Naturally Infected Dogs from Northeast Brazil","volume":"30","author":"Reis","year":"2019","journal-title":"Vet. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s11262-015-1220-y","article-title":"Genetic characterization of Amazonian bovine papillomavirus reveals the existence of four new putative types","volume":"51","author":"Daudt","year":"2015","journal-title":"Virus Genes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tvjl.2019.05.016","article-title":"Canine papillomavirus types 1 and 2 in classical papillomas: High abundance, different morphological associations and frequent co-infections","volume":"250","author":"Lange","year":"2019","journal-title":"Vet. J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1177\/0898756418774575","article-title":"Malignant transformation of a canine papillomavirus type 1-induced persistent oral papilloma in a 3-year-old dog","volume":"35","author":"Legendre","year":"2018","journal-title":"J. Vet. Dent."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e01006-16","DOI":"10.1128\/genomeA.01006-16","article-title":"Complete genome sequences of two isolates of Canis familiaris oral papillomavirus from South Africa","volume":"4","author":"Regnard","year":"2016","journal-title":"Genome Announc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"57-e20","DOI":"10.1111\/vde.12189","article-title":"Antibody titres against canine papillomavirus 1 peak around clinical regression in naturally occurring oral papillomatosis","volume":"26","author":"Sancak","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1177\/1040638714567191","article-title":"Multiple oral carcinomas associated with a novel papillomavirus in a dog","volume":"27","author":"Munday","year":"2015","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_39","first-page":"2025","article-title":"Phylogenetic analysis of papillomaviruses in dogs from southern Brazil: Molecular epidemiology and investigation of mixed infections and spillover events","volume":"55","author":"Merchioratto","year":"2024","journal-title":"Vet. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Souza, F.d.A., Daudt, C., Lins, A.d.M.C., dos Santos, I.R., Tomaya, L.Y.C., Lima, A.d.S., Reis, E.M.B., Satrapa, R.A., Driemeier, D., and Bagon, A. (2025). Characterization of Papillomatous Lesions and Genetic Diversity of Bovine Papillomavirus from the Amazon Region. Viruses, 17.","DOI":"10.20944\/preprints202504.0818.v1"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kim, W.S., Vinayak, A., and Powers, B. (2021). Comparative Review of Malignant Melanoma and Histologically Well-Differentiated Melanocytic Neoplasm in the Oral Cavity of Dogs. Vet. Sci., 8.","DOI":"10.3390\/vetsci8110261"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1111\/vde.13196","article-title":"Dermoscopic features of benign sebaceous proliferations in dogs: Description, assessment and inter-observer agreement","volume":"34","author":"Scarampella","year":"2023","journal-title":"Vet. Dermatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1177\/03009858231189205","article-title":"Automated diagnosis of 7 canine skin tumors using machine learning on H&E-stained whole slide images","volume":"60","author":"Wilm","year":"2023","journal-title":"Vet. Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1177\/0300985816688754","article-title":"Clinical and Histopathologic Characterization of Canine Chronic Ulcerative Stomatitis","volume":"54","author":"Anderson","year":"2017","journal-title":"Vet. Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.2460\/javma.23.01.0002","article-title":"Managing recurrent otitis externa in dogs: What have we learned and what can we do better?","volume":"261","author":"Nuttall","year":"2023","journal-title":"J. Am. Vet. Med. Assoc."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/8\/1811\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:22:01Z","timestamp":1760034121000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/8\/1811"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,2]]},"references-count":45,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["microorganisms13081811"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13081811","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,2]]}}}