{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:20:27Z","timestamp":1790176827286,"version":"4.1.0"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32470448"],"award-info":[{"award-number":["32470448"]}]},{"name":"National Natural Science Foundation of China","award":["32270444"],"award-info":[{"award-number":["32270444"]}]},{"name":"National Natural Science Foundation of China","award":["32370443"],"award-info":[{"award-number":["32370443"]}]},{"name":"National Natural Science Foundation of China","award":["32170530"],"award-info":[{"award-number":["32170530"]}]},{"name":"National Natural Science Foundation of China","award":["2022KJ177"],"award-info":[{"award-number":["2022KJ177"]}]},{"name":"Youth Innovation Team in Colleges and Universities of Shandong Province","award":["32470448"],"award-info":[{"award-number":["32470448"]}]},{"name":"Youth Innovation Team in Colleges and Universities of Shandong Province","award":["32270444"],"award-info":[{"award-number":["32270444"]}]},{"name":"Youth Innovation Team in Colleges and Universities of Shandong Province","award":["32370443"],"award-info":[{"award-number":["32370443"]}]},{"name":"Youth Innovation Team in Colleges and Universities of Shandong Province","award":["32170530"],"award-info":[{"award-number":["32170530"]}]},{"name":"Youth Innovation Team in Colleges and Universities of Shandong Province","award":["2022KJ177"],"award-info":[{"award-number":["2022KJ177"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Bacillus paranthracis, a species of the genus Bacillus, is a Gram-positive bacterium classified as an opportunistic pathogen that can cause foodborne diarrhea and other intestinal diseases in humans and various animals. To date, there has been limited research on B. paranthracis, and there are few records of this bacterium being isolated from animal intestines. In this study, a strain named Qf-1 was isolated and purified from faecal samples of mink. Through culturomics, 16S rRNA gene sequencing, whole-genome sequencing, and average nucleotide identity (ANI) analysis, the strain was confirmed to be B. paranthracis. Whole-genome sequencing revealed that the strain has a genome size of 5.27 Mb, comprising one chromosome (5,224,739 bp) and one plasmid (51,964 bp). Functional annotation of its genome identified multiple potential pathogenic factors associated with pneumonia, including the key genes AsbD and AsbF, which facilitate bacterial colonisation of the lungs and trigger inflammatory responses, as well as EsxB and EsxL, which exacerbate lung inflammation and promote infection spread. Comparative genomics analysis revealed that this strain shares a close evolutionary relationship with previously reported B. paranthracis strains. The structure and function of the bacterial genes were analyzed in depth using multi-omics methods. Through mouse pathogenicity experiments, it was found that this bacterium may cause pneumonia and enteritis in mice. We predict that it may also pose a threat to the health of the mink. These research findings contribute to the establishment of a stable experimental model between pathogens and mink hosts, laying the foundation for further elucidating their pathogenicity and pathogenic mechanisms. This is of great significance for the diagnosis and prevention of bacterial diseases in mink in the future.<\/jats:p>","DOI":"10.3390\/microorganisms13092106","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T19:16:17Z","timestamp":1757445377000},"page":"2106","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Whole-Genome Analysis of Bacillus paranthracis Qf-1 Isolated from Mink (Neogale vison)"],"prefix":"10.3390","volume":"13","author":[{"given":"Haotian","family":"Cai","sequence":"first","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-3336","authenticated-orcid":false,"given":"Yao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2036-5184","authenticated-orcid":false,"given":"Xibao","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongquan","family":"Shang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinguo","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilai","family":"Sha","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghai","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qufu Normal University, Qufu 273100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Qiu, J., Nituch, L.A., Bowman, J., Beauclerc, K.B., and Schulte-Hostedde, A.I. (2011). Mink Farms Predict Aleutian Disease Exposure in Wild American Mink. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021693"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"122307","DOI":"10.1016\/j.envpol.2023.122307","article-title":"Tracking pollution from fur farms using forensic paleolimnology","volume":"335","author":"Kissinger","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_3","first-page":"256","article-title":"Molecular epidemiology, antimicrobial susceptibility, and pulsed-field gel electrophoresis genotyping of Pseudomonas aeruginosa isolates from mink","volume":"82","author":"Zhao","year":"2018","journal-title":"Can. J. Vet. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s12560-014-9155-3","article-title":"Detection and Characterization of Avastrovirus Associated with Diarrhea Isolated from Minks in China","volume":"6","author":"Sun","year":"2014","journal-title":"Food Environ. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.vetmic.2014.02.025","article-title":"The identification, typing, and antimicrobial susceptibility of Pseudomonas aeruginosa isolated from mink with hemorrhagic pneumonia","volume":"170","author":"Qi","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_6","first-page":"824","article-title":"Drug Resistance and Genetic Relatedness of Escherichia coli from Mink in Northeast China","volume":"43","author":"Li","year":"2023","journal-title":"Pak. Vet. J."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wang, J., Shang, Y., Guo, S., Diao, F., Yu, J., Wei, X., Zhao, Y., Jiang, S., and Xie, Z. (2017). Serotype and virulence genes of Klebsiella pneumoniae isolated from mink and its pathogenesis in mice and mink. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-17681-8"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chen, Y., Cai, H., Wu, X., Wang, X., Shang, Y., Wei, Q., Sha, W., Qi, Y., Liu, S., and Zhang, H. (2025). Identification and Pathogenicity Analysis of Huaxiibacter chinensis Qf-1 in Mink (Neogale vison). Microorganisms, 13.","DOI":"10.3390\/microorganisms13071604"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wang, G.S., Du, Y., Wu, J.Q., Tian, F.L., Yu, X.J., and Wang, J.B. (2018). Vaccine resistant pseudorabies virus causes mink infection in China. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1334-2"},{"key":"ref_10","first-page":"10","article-title":"The Bacillus cereus Group: Bacillus Species with Pathogenic Potential","volume":"7","author":"Lereclus","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1099\/ijsem.0.001821","article-title":"Proposal of nine novel species of the Bacillus cereus group","volume":"67","author":"Liu","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Matson, M.J., Anzick, S.L., Feldmann, F., Martens, C.A., Drake, S.K., Feldmann, H., Massaquoi, M., Chertow, D.S., and Munster, V.J. (2020). Bacillus paranthracis Isolate from Blood of Fatal Ebola Virus Disease Case. Pathogens, 9.","DOI":"10.3390\/pathogens9060475"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sk\u00f3ra, M., Zaj\u0105c, M., Kwit, R., Skar\u017cy\u0144ska, M., Pasim, P., Mikos-Wojewoda, E., Bomba, A., Giza, A., Chesneau, O., and Hendriksen, R.S. (2022). Draft Genome Sequences of Six Isolates of the Bacillus cereus Group Isolated from Pet Reptiles. Microbiol. Resour. Announc., 11.","DOI":"10.1128\/mra.00385-22"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2348498","DOI":"10.1080\/22221751.2024.2348498","article-title":"A rare waterborne outbreak of Bacillus paranthracis in Shandong province, China, 2020: Epidemiologic survey, genomic insights, and virulence characteristics","volume":"13","author":"Wang","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"104867","DOI":"10.1016\/j.meegid.2021.104867","article-title":"Genomic and pathogenicity of a Bacillus paranthracis isolated from book page surface","volume":"92","year":"2021","journal-title":"Infect. Genet. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/MRA.00662-19","article-title":"Genome Sequence Announcement of Bacillus paranthracis Strain ICIS-279, Isolated from Human Intestine","volume":"8","author":"Bukharin","year":"2019","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Carroll, L.M., Wiedmann, M., Mukherjee, M., Nicholas, D.C., Mingle, L.A., Dumas, N.B., Cole, J.A., and Kovac, J. (2019). Characterization of Emetic and Diarrheal Bacillus cereus Strains From a 2016 Foodborne Outbreak Using Whole-Genome Sequencing: Addressing the Microbiological, Epidemiological, and Bioinformatic Challenges. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00144"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zhang, P., Yao, H., Ji, L., Dong, F., Xu, D., and Yan, W. (2025). Epidemiological and genomic characterization of a rare foodborne outbreak caused by Bacillus paranthracis in Huzhou, China, 2024. Int. J. Food Microbiol., 439.","DOI":"10.1016\/j.ijfoodmicro.2025.111265"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101741","DOI":"10.1016\/j.xpro.2022.101741","article-title":"Non-canonical inflammasome activation analysis in a mouse model of Citrobacter rodentium infection","volume":"3","author":"Tsutsuki","year":"2022","journal-title":"STAR Protoc."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wo\u017aniak, M., Ga\u0142\u0105zka, A., Ty\u015bkiewicz, R., and Jaroszuk-\u015acise\u0142, J. (2019). Endophytic Bacteria Potentially Promote Plant Growth by Synthesizing Different Metabolites and their Phenotypic\/Physiological Profiles in the Biolog GEN III MicroPlate(TM) Test. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20215283"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.micpath.2017.10.019","article-title":"First isolation and characterization of the pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome in the indigenous Pelophylax ridibundus of Al-Ahsaa, Saudi Arabia","volume":"117","author":"Khalifa","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_22","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1126\/science.164.3881.788","article-title":"Non-Darwinian evolution","volume":"164","author":"King","year":"1969","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lindgreen, S. (2012). AdapterRemoval: Easy cleaning of next-generation sequencing reads. BMC Res. Notes, 5.","DOI":"10.1186\/1756-0500-5-337"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/2047-217X-1-18","article-title":"SOAPdenovo2: An empirically improved memory-efficient short-read de novo assembler","volume":"1","author":"Luo","year":"2012","journal-title":"Gigascience"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1093\/bioinformatics\/btu661","article-title":"A5-miseq: An updated pipeline to assemble microbial genomes from Illumina MiSeq data","volume":"31","author":"Coil","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Walker, B.J., Abeel, T., Shea, T., Priest, M., Abouelliel, A., Sakthikumar, S., Cuomo, C.A., Zeng, Q., Wortman, J., and Young, S.K. (2014). Pilon: An integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112963"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1099\/ijs.0.054171-0","article-title":"Integrating genomics into the taxonomy and systematics of the Bacteria and Archaea","volume":"64","author":"Chun","year":"2014","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"D274","DOI":"10.1093\/nar\/gkaa1018","article-title":"COG database update: Focus on microbial diversity, model organisms, and widespread pathogens","volume":"49","author":"Galperin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: Genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"Kanehisa","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: Hierarchical and refined dataset for big data analysis\u201410 years on","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_36","first-page":"D517","article-title":"CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: Multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Edgar, R.C. (2004). MUSCLE: A multiple sequence alignment method with reduced time and space complexity. BMC Bioinform., 5.","DOI":"10.1186\/1471-2105-5-113"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kurtz, S., Phillippy, A., Delcher, A.L., Smoot, M., Shumway, M., Antonescu, C., and Salzberg, S.L. (2004). Versatile and open software for comparing large genomes. Genome Biol., 5.","DOI":"10.1186\/gb-2004-5-2-r12"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Sornchuer, P., Saninjuk, K., Amonyingcharoen, S., Ruangtong, J., Thongsepee, N., Martviset, P., Chantree, P., and Sangpairoj, K. (2024). Whole Genome Sequencing Reveals Antimicrobial Resistance and Virulence Genes of Both Pathogenic and Non-Pathogenic B. cereus Group Isolates from Foodstuffs in Thailand. Antibiot., 13.","DOI":"10.3390\/antibiotics13030245"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.3390\/toxins2071690","article-title":"Production, Secretion and Biological Activity of Bacillus cereus Enterotoxins","volume":"2","author":"Senesi","year":"2010","journal-title":"Toxins"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/CMR.00073-09","article-title":"Bacillus cereus, a Volatile Human Pathogen","volume":"23","author":"Bottone","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_43","first-page":"303","article-title":"Genomics of the group of organisms","volume":"29","author":"Rasko","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"458","DOI":"10.3855\/jidc.20080","article-title":"Can Bacillus paranthracis cause bacteremia in a T-ALL patient? WGS-based diagnosis","volume":"19","author":"Polat","year":"2025","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"7004","DOI":"10.1128\/JB.00458-08","article-title":"ESAT-6-Like Protein Secretion in Bacillus anthracis","volume":"190","author":"Garufi","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"17133","DOI":"10.1073\/pnas.0808118105","article-title":"Structural and functional analysis of AsbF: Origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis","volume":"105","author":"Pfleger","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1128\/AEM.02728-07","article-title":"Identification of siderophore biosynthesis genes essential for growth of Aeromonas salmonicida under iron limitation conditions","volume":"74","author":"Najimi","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"16058","DOI":"10.1074\/jbc.M112.359349","article-title":"Functional and Structural Analysis of the Siderophore Synthetase AsbB through Reconstitution of the Petrobactin Biosynthetic Pathway from Bacillus anthracis","volume":"287","author":"Nusca","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_49","unstructured":"Nusca, T.D. (2012). Detailed Analysis of Biosynthetic Components for the Virulence-Associated Siderophore Petrobactin from Bacillus anthracis. [Ph.D. Thesis, University of Michigan]."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1128\/JB.01609-08","article-title":"Role of Clp Proteins in Expression of Virulence Properties of Streptococcus mutans","volume":"191","author":"Kajfasz","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1002\/mbo3.251","article-title":"Alternative modes of biofilm formation by plant\u2014Associated Bacillus cereus","volume":"4","author":"Gao","year":"2015","journal-title":"MicrobiologyOpen"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s11274-007-9408-5","article-title":"Expression and characterization of inhA gene from Bacillus thuringiensis 8010","volume":"23","author":"Yu","year":"2007","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1099\/00221287-147-7-1805","article-title":"Identification of genes involved in the activation of the Bacillus thuringiensis inhA metalloprotease gene at the onset of sporulation","volume":"147","author":"Grandvalet","year":"2001","journal-title":"Microbiology"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/9\/2106\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:42:44Z","timestamp":1760035364000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/9\/2106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,9]]},"references-count":53,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["microorganisms13092106"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13092106","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,9]]}}}