{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:45Z","timestamp":1787029005827,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T00:00:00Z","timestamp":1758672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The remarkable metabolic adaptability of Bacillus velezensis, including efficient nutrient use, spore formation, and the secretion of antimicrobial peptides, supports its expanding role in biotechnological applications ranging from crop protection to probiotic development. In this study, the halotolerant strain 24.5 was identified as B. velezensis through 16S rDNA and gyrA gene sequencing. PCR analyses confirmed the presence of genes responsible for polyketides, lipopeptides, and dipeptides biosynthesis. These results indicate the potential for the production of structurally diverse bioactive metabolites. Strain 24.5 demonstrated remarkable antimicrobial activity against 19 bacterial pathogens and three Candida species (p &lt; 0.05). The study demonstrated high survival rates under simulated gastrointestinal conditions, suggesting strong adaptability for gut colonization. Antioxidant evaluation revealed DPPH radical scavenging activities of 34.68% for intact cells and 18.47% for the cell-free extract (p &lt; 0.05). The enzymatic profile highlighted versatile metabolic functions, supporting its multifaceted probiotic potential. Auto-aggregation reached 84.42% at 24 h, and high hydrophobicity toward hexane (71.62%) supported adhesion potential. Antibiotic susceptibility profiling showed sensitivity or intermediate susceptibility to 22 of 24 tested antibiotics (p &lt; 0.05). No haemolytic activity was detected, supporting its safety profile. Overall, these results emphasise the adaptability and multifunctional properties of Bacillus velezensis strain 24.5, highlighting its potential as a promising probiotic candidate for applications in food safety and biotechnology.<\/jats:p>","DOI":"10.3390\/microorganisms13102240","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T07:46:49Z","timestamp":1758786409000},"page":"2240","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In Vitro Evaluation of Halotolerant Bacillus velezensis 24.5 as a Promising Probiotic with Broad-Spectrum Antimicrobial Activity"],"prefix":"10.3390","volume":"13","author":[{"given":"Filofteia Camelia","family":"Digu\u021b\u0103","sequence":"first","affiliation":[{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine, 59 Marasti Blvd., 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3912-1120","authenticated-orcid":false,"given":"Radu Cristian","family":"Toma","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine, 59 Marasti Blvd., 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7568-9952","authenticated-orcid":false,"given":"Florentina","family":"Matei","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine, 59 Marasti Blvd., 011464 Bucharest, Romania"},{"name":"Faculty of Food Industry and Tourism, Transilvania University of Bra\u0219ov, 148 Castelului Street, 500014 Bra\u0219ov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,24]]},"reference":[{"key":"ref_1","unstructured":"FAO\/WHO (May, January 30). Guidelines for the Evaluation of Probiotics in Food. Proceedings of the Joint FAO\/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food, London, ON, Canada."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic","volume":"11","author":"Hill","year":"2014","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100185","DOI":"10.1016\/j.afres.2022.100185","article-title":"Current status of probiotic and related health benefits","volume":"2","author":"Das","year":"2022","journal-title":"Appl. Food Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Dobreva, L., Atanasova, N., Donchev, P., Krumova, E., Abrashev, R., Karakirova, Y., Mladenova, R., Tolchkov, V., Ralchev, N., and Dishliyska, V. (2024). Candidate-Probiotic Lactobacilli and Their Postbiotics as Health-Benefit Promoters. Microorganisms, 12.","DOI":"10.3390\/microorganisms12091910"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Maftei, N.-M., Raileanu, C.R., Balta, A.A., Ambrose, L., Boev, M., Marin, D.B., and Lisa, E.L. (2024). The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties. Microorganisms, 12.","DOI":"10.3390\/microorganisms12020234"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Baliyan, N., Kumari, M., Kumari, P., Dindhoria, K., Mukhia, S., Kumar, S., and Kumar, R. (2022). Probiotics in fermented products and supplements. Current Developments in Bioengineering, Elsevier.","DOI":"10.1016\/B978-0-12-823506-5.00014-X"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Pompa, L., Montanari, A., Tomassini, A., Bianchi, M.M., Aureli, W., Miccheli, A., Uccelletti, D., and Schifano, E. (2023). In Vitro Probiotic Properties and In Vivo Anti-Ageing Effects of Lactoplantibacillus plantarum PFA2018AU Strain Isolated from Carrots on Caenorhabditis elegans. Microorganisms, 11.","DOI":"10.3390\/microorganisms11041087"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100139","DOI":"10.1016\/j.gpeds.2024.100139","article-title":"Bifidobacteria, Lactobacilli\u2026 when, how and why to use them","volume":"8","author":"Mariarosaria","year":"2024","journal-title":"Glob. Pediatr."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Pristavu, M.-C., Digu\u021b\u0103, F.C., Aldea, A.C., Badea, F., Dragoi Cudalbeanu, M., Ortan, A., and Matei, F. (2025). Functional Profiling of Enterococcus and Pediococcus Strains: An In Vitro Study on Probiotic and Postbiotic Properties. Microorganisms, 13.","DOI":"10.3390\/microorganisms13061348"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pais, P., Almeida, V., Y\u0131lmaz, M., and Teixeira, M.C. (2020). Saccharomyces boulardii: What makes it tick as successful probiotic?. J. Fungi, 6.","DOI":"10.3390\/jof6020078"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Pacheco, P., Pintado, C., Briones P\u00e9rez, A., and Ar\u00e9valo-Villena, M. (2021). Potential Probiotic Strains of Saccharomyces and Non-Saccharomyces: Functional and Biotechnological Characteristics. J. Fungi, 7.","DOI":"10.3390\/jof7030177"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Pacheco, P., Ramos Monge, I.M., Fern\u00e1ndez-Gonz\u00e1lez, M., Poveda Colado, J.M., and Ar\u00e9valo-Villena, M. (2021). Safety Evaluation of Yeasts With Probiotic Potential. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.659328"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Vr\u00eenceanu, C.-R., Digu\u021b\u0103, F.C., Cudalbeanu, M.D., Ortan, A., Mihai, C., B\u0103rbulescu, I.D., Fr\u00eencu, M., Begea, M., Matei, F., and Teodorescu, R.I. (2025). Exploring the Potential of Torulaspora delbrueckii, Starmerella bacillaris, and Saccharomyces cerevisiae as a Probiotic Starter for Craft Beer Production. Foods, 14.","DOI":"10.3390\/foods14091608"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e09725","DOI":"10.1016\/j.heliyon.2022.e09725","article-title":"Probiotics, their prophylactic and therapeutic applications in human health development: A review of the literature","volume":"8","author":"Tegegne","year":"2022","journal-title":"Heliyon"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1318034","DOI":"10.1080\/16546628.2017.1318034","article-title":"Some current applications, limitations and future perspectives of lactic acid bacteria as probiotics","volume":"61","author":"Evivie","year":"2017","journal-title":"Food Nutr. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6455","DOI":"10.1080\/10408398.2023.2169858","article-title":"Commercial probiotic products in public health: Current status and potential limitations","volume":"64","author":"Liang","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.26599\/FSHW.2022.9250148","article-title":"Research advance of Bacillus velezensis: Bioinformatics, characteristics, and applications","volume":"13","author":"Su","year":"2024","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Fan, B., Blom, J., Klenk, H.-P., and Borriss, R. (2017). Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis Form an \u201cOperational Group B. amyloliquefaciens\u201d within the B. subtilis Species Complex. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00022"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Payne, J., Bellmer, D., Jadeja, R., and Muriana, P. (2024). The Potential of Bacillus Species as Probiotics in the Food Industry: A Review. Foods, 13.","DOI":"10.3390\/foods13152444"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s12275-021-1161-1","article-title":"Potential of Bacillus velezensis as a Probiotic in Animal Feed: A Review","volume":"59","author":"Khalid","year":"2021","journal-title":"J. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Brutscher, L.M., Gebrechristos, S., Garvey, S.M., and Spears, J.L. (2024). Genetic and Phenotypic Characterization of Bacillus velezensis Strain BV379 for Human Probiotic Applications. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030436"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1080\/10408398.2022.2118659","article-title":"Bacillus-derived probiotics: Metabolites and mechanisms involved in bacteria\u2013host interactions","volume":"64","author":"Lu","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es Sousa, E., Munis Campos, G., Quaresma, L.S., Mendon\u00e7a Mota, T.F., de Castilhos Ghisi, N., Camargos Gomes, G., Viegas Santos, R.C.V., de Souza, B.G.R., Gu\u00e9don, \u00c9., and de Castro Soares, S. (2025). Exploring Bacillus velezensis in biomedical context: A systematic review. Acad. Mol. Biol. Genom., 2.","DOI":"10.20935\/AcadMolBioGen7598"},{"key":"ref_24","unstructured":"FDA GRN (2025, July 07). GRAS Notification 1131, Available online: https:\/\/www.fda.gov\/media\/175167\/download."},{"key":"ref_25","first-page":"5965","article-title":"Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 11: Suitability of taxonomic units notified to EFSA until September 2019","volume":"18","author":"Koutsoumanis","year":"2020","journal-title":"EFSA J."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"V\u00f6r\u00f6s, M., Manczinger, L., Kredics, L., Szekeres, A., Shine, K., Alharbi, N.S., Khaled, J.M., and V\u00e1gv\u00f6lgyi, C. (2019). Influence of agro-environmental pollutants on a biocontrol strain of Bacillus velezensis. Microbiol. Open, 8.","DOI":"10.1002\/mbo3.660"},{"key":"ref_27","first-page":"641","article-title":"Bacterial strains involved in soilborne phytopathogens inhibition","volume":"64","author":"Cornea","year":"2021","journal-title":"Sci. Pap. Ser. A Agron."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Boiu-Sicuia, O.-A., Toma, R.C., Digu\u021b\u0103, C.F., Matei, F., and Cornea, C.P. (2023). In Vitro Evaluation of Some Endophytic Bacillus to Potentially Inhibit Grape and Grapevine Fungal Pathogens. Plants, 12.","DOI":"10.3390\/plants12132553"},{"key":"ref_29","first-page":"1744","article-title":"Halotolerant Bacillus amyloliquefaciens 24.5 is helpful as a biological agent to control phyto-pathogenic fungi","volume":"25","author":"Proca","year":"2020","journal-title":"Rom. Biotechnol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1007\/s12602-024-10215-5","article-title":"Targeted Screening of Fiber Degrading Bacteria with Probiotic Function in Herbivore Feces","volume":"17","author":"Chen","year":"2025","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"107645","DOI":"10.1016\/j.micpath.2025.107645","article-title":"Probiogenomic insights into Bacillus velezensis MFSS1 for controlling aquaculture pathogens","volume":"205","author":"Mustafa","year":"2025","journal-title":"Microb. Pathog."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"da Rosa, C.E., Pinilla, C.M.B., Toss, L.D., and Brandelli, A. (2025). In Silico and In Vitro Characterization of Bacillus velezensis P45: Screening for a Novel Probiotic Candidate. Foods, 14.","DOI":"10.3390\/foods14132334"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1093\/femsre\/fuy028","article-title":"Secondary metabolite production and the safety of industrially important members of the Bacillus subtilis group","volume":"42","author":"Harwood","year":"2018","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Rabbee, M.F., Ali, M.S., Choi, J., Hwang, B.S., Jeong, S.C., and Baek, K.-h. (2019). Bacillus velezensis: A Valuable Member of Bioactive Molecules within Plant Microbiomes. Molecules, 24.","DOI":"10.3390\/molecules24061046"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ngalimat, M.S., Yahaya, R.S.R., Baharudin, M.M.A.-a., Yaminudin, S.M., Karim, M., Ahmad, S.A., and Sabri, S. (2021). A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens. Microorganisms, 9.","DOI":"10.3390\/microorganisms9030614"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7177","DOI":"10.1007\/s00253-020-10713-w","article-title":"Quorum sensing signal autoinducer-2 promotes root colonization of Bacillus velezensis SQR9 by affecting biofilm formation and motility","volume":"104","author":"Xiong","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Alenezi, F.N., Slama, H.B., Bouket, A.C., Cherif-Silini, H., Silini, A., Luptakova, L., Nowakowska, J.A., Oszako, T., and Belbahri, L. (2021). Bacillus velezensis: A Treasure House of Bioactive Compounds of Medicinal, Biocontrol and Environmental Importance. Forests, 12.","DOI":"10.3390\/f12121714"},{"key":"ref_38","first-page":"100392","article-title":"Secondary Metabolites from Bacillus spp. probiotics as potential treatments for multidrug-resistant pathogens: A comprehensive review","volume":"8","author":"Nicolas","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Danilova, I., and Sharipova, M. (2020). The practical potential of bacilli and their enzymes for industrial production. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01782"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Li, C., Li, S., Dang, G., Jia, R., Chen, S., Deng, X., Liu, G., Beckers, Y., and Cai, H. (2023). Screening and characterization of Bacillus velezensis LB-Y-1 toward selection as a potential probiotic for poultry with multi-enzyme production property. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1143265"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Prazdnova, E.V., Mazanko, M.S., Shevchenko, V.N., Skripnichenko, R.V., Kulikov, M.P., Golovko, L.S., Grigoriev, V.A., Maltseva, T.A., Kulikova, D.B., and Rudoy, D.V. (2025). Genomic Characterization of Four Novel Probiotic Strains with Enzymatic Activity and Their Effects on Carp (Cyprinus carpio). Animals, 15.","DOI":"10.3390\/ani15131998"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Hrabar, J., Babi\u0107, I., Jozi\u0107, S., Trumbi\u0107, \u017d., Pioppi, A., Nielsen, L.J.D., Maravi\u0107, A., Toma\u0161evi\u0107, T., and Kovacs, \u00c1.T. (2025). Mladineo, I. Prospecting microbiota of Adriatic fish: Bacillus velezensis as a potential probiotic candidate. Anim. Microbiome, 7.","DOI":"10.1186\/s42523-025-00429-5"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1023\/A:1026555830014","article-title":"Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences","volume":"78","author":"Chun","year":"2000","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_45","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1007\/s00253-009-2176-4","article-title":"New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction","volume":"85","author":"Tapi","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s00253-008-1520-4","article-title":"Isolation and partial characterization of Bacillus subtilis ME488 for suppression of soilborne pathogens of cucumber and pepper","volume":"80","author":"Chung","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1139\/W09-067","article-title":"Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry","volume":"55","author":"Athukorala","year":"2009","journal-title":"Can. J. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/jam.12214","article-title":"Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food","volume":"115","author":"Nielsen","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_50","first-page":"609","article-title":"Selected plant protection Bacillus strains increase food safeness by inhibiting human pathogenic bacteria","volume":"40","author":"Toma","year":"2023","journal-title":"Rom. Agric. Res."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Borah, T., Gogoi, B., Khataniar, A., Gogoi, M., Das, A., and Borah, D. (2019). Probiotic characterization of indigenous Bacillus velezensis strain DU14 isolated from Apong, a traditionally fermented rice beer of Assam. Biocatal. Agric. Biotechnol., 18.","DOI":"10.1016\/j.bcab.2019.01.046"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Coulibaly, W.H., Kouadio, N.R., Camara, F., Digu\u021b\u0103, C., and Matei, F. (2023). Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast. BMC Microbiol., 23.","DOI":"10.1186\/s12866-023-02899-6"},{"key":"ref_53","first-page":"197","article-title":"Screening of halotolerant bacteria producing hydrolytic enzymes with biotechnological applications","volume":"XXIV","author":"Proca","year":"2020","journal-title":"Sci. Bull. Ser. F Biotechnol."},{"key":"ref_54","unstructured":"Clinical and Laboratory Standards Institute (2021). Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [31st ed.]. CLSI document M100."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Kumar, P., Kizhakkedathu, J.N., and Straus, S.K. (2018). Antimicrobial peptides: Diversity, mechanism of action and strategies to improve the activity and biocompatibility in vivo. Biomolecules, 8.","DOI":"10.3390\/biom8010004"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1080\/13102818.2019.1611385","article-title":"Antimicrobial activity, mechanism of action, and methods for stabilisation of defensins as new therapeutic agents","volume":"33","author":"Amerikova","year":"2019","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"106163","DOI":"10.1016\/j.micpath.2023.106163","article-title":"Characterization of Bacillus spp. isolated from the intestines of Rhynchocypris lagowskii as a potential probiotic and their effects on fish pathogens","volume":"180","author":"Elsadek","year":"2023","journal-title":"Microb. Pathog."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"115596","DOI":"10.1016\/j.lwt.2023.115596","article-title":"Probiotic Bacillus Strains Inhibit Growth, Biofilm Formation, and Virulence Gene Expression of Listeria monocytogenes","volume":"191","author":"Noshad","year":"2024","journal-title":"LWT"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Nwagu, T.N., Ugwuodo, C.J., Onwosi, C.O., Inyima, O., Uchendu, O.C., and Akpuru, C. (2020). Evaluation of the probiotic attributes of Bacillus strains isolated from traditional fermented African locust bean seeds (Parkia biglobosa), \u201cdaddawa\u201d. Ann. Microbiol., 70.","DOI":"10.1186\/s13213-020-01564-x"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s12272-025-01541-5","article-title":"A review on bacteria-derived antioxidant metabolites: Their production, purification, characterization, potential applications, and limitations","volume":"48","author":"Arslan","year":"2025","journal-title":"Arch. Pharm. Res."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Khan, M.M., Kim, Y.K., Bilkis, T., Suh, J.-W., Lee, D.Y., and Yoo, J.C. (2020). Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from Bacillus velezensis. Antioxidants, 9.","DOI":"10.3390\/antiox9100934"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1007\/s12602-020-09650-x","article-title":"Probiotic Potential of Bacillus Strains Isolated from an Acidic Fermented Food Idli","volume":"12","author":"Shivangi","year":"2020","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"101254","DOI":"10.1016\/j.aqrep.2022.101254","article-title":"Evaluation of probiotic characteristics and whole genome analysis of Bacillus velezensis R-71003 isolated from the intestine of common carp (Cyprinus carpio L.) for its use as a probiotic in aquaculture","volume":"25","author":"Kang","year":"2022","journal-title":"Aquac. Rep."},{"key":"ref_64","first-page":"364","article-title":"Probiotic Potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread","volume":"17","author":"Worobo","year":"2023","journal-title":"Probiotics Antimicrob. Proteins"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/10\/2240\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:49:11Z","timestamp":1760035751000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/10\/2240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,24]]},"references-count":64,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["microorganisms13102240"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13102240","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,24]]}}}