{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:32:17Z","timestamp":1788449537864,"version":"build-2803163510"},"reference-count":68,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:00:00Z","timestamp":1759017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160833"],"award-info":[{"award-number":["32160833"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160834"],"award-info":[{"award-number":["32160834"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Corps Science and Technology Program","award":["2024ZD002"],"award-info":[{"award-number":["2024ZD002"]}]},{"name":"Major Science and Technology Project of Xinjiang Academy of Agricultural Reclamation Sciences","award":["NCG202408"],"award-info":[{"award-number":["NCG202408"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Listeria monocytogenes (L. monocytogenes) is a Gram-positive intracellular pathogen capable of causing severe infections. The Listeria pathogenicity island 4 (LIPI-4) encodes a phosphotransferase system (PTS) with its EIIB component playing a critical role in carbohydrate phosphorylation and virulence. However, the precise function of EIIB in virulence regulation across diverse pathogenic strains remains unclear. Here, we generated an EIIB deletion mutant (LM873\u0394EIIB) and its complemented strain (CLM873\u0394EIIB) from the low-virulence strain LM873, and performed comparative analyses with the high-virulence strain LM928 and its corresponding mutants. Deletion of EIIB differentially modulated biofilm formation: suppressing it in LM928 while enhancing it in LM873, accompanied by corresponding transcriptional changes in biofilm-associated and virulence genes. Both mutants exhibited impaired hemolytic activity, whereas motility attenuation was specific to LM928\u0394EIIB. At the cellular level, LM873\u0394EIIB enhanced adhesion to and invasion of Caco-2 but impaired intracellular proliferation in JEG-3; In contrast, LM928\u0394EIIB promoted Caco-2 invasion while attenuating JEG-3 adhesion, invasion, and intracellular replication, as well as reducing invasion and proliferation in RAW264.7 macrophage. Animal experiments demonstrated that EIIB deletion attenuated LM928 colonization in the liver and spleen, but had no significant impact on LM873. Collectively, our findings establish EIIB as a strain-dependent virulence regulator in L. monocytogenes, particularly modulating biofilm formation and host\u2013pathogen interactions.<\/jats:p>","DOI":"10.3390\/microorganisms13102274","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T10:49:34Z","timestamp":1759142974000},"page":"2274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The PTS EIIB Component Drives Strain-Specific Virulence in Listeria monocytogenes: Divergent Regulation of Biofilm Formation and Host Infection in High- and Low-Virulence Strains"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5293-2279","authenticated-orcid":false,"given":"Lu","family":"Liu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3778-1539","authenticated-orcid":false,"given":"Caixia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixuan","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yatao","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongke","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifeng","family":"Luo","sequence":"additional","affiliation":[{"name":"Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Du","sequence":"additional","affiliation":[{"name":"Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichao","family":"Kang","sequence":"additional","affiliation":[{"name":"Analysis and Testing Center, Xinjiang Academy of Agriculture and Reclamation Sciences, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/nrmicro.2017.126","article-title":"Listeria monocytogenes: Towards a Complete Picture of Its Physiology and Pathogenesis","volume":"16","author":"Radoshevich","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne Illness Acquired in the United States\u2014Major Pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0168-1605(03)00326-X","article-title":"Listeria monocytogenes and Listeriosis: A Review of Hazard Characterisation for Use in Microbiological Risk Assessment of Foods","volume":"92","author":"McLauchlin","year":"2004","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s41522-021-00189-5","article-title":"Biofilm-Isolated Listeria monocytogenes Exhibits Reduced Systemic Dissemination at the Early (12\u201324 h) Stage of Infection in a Mouse Model","volume":"7","author":"Bai","year":"2021","journal-title":"NPJ Biofilms Microbiomes"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Macleod, J., Beeton, M.L., and Blaxland, J. (2022). An Exploration of Listeria monocytogenes, Its Influence on the UK Food Industry and Future Public Health Strategies. Foods, 11.","DOI":"10.3390\/foods11101456"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1080\/21505594.2021.1975526","article-title":"Pathogenicity and Virulence of Listeria monocytogenes: A Trip from Environmental to Medical Microbiology","volume":"12","author":"Quereda","year":"2021","journal-title":"Virulence"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Osek, J., and Wieczorek, K. (2022). Listeria monocytogenes\u2014How This Pathogen Uses Its Virulence Mechanisms to Infect the Hosts. Pathogens, 11.","DOI":"10.3390\/pathogens11121491"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/j.tim.2021.01.008","article-title":"Making Sense of the Biodiversity and Virulence of Listeria monocytogenes","volume":"29","author":"Disson","year":"2021","journal-title":"Trends Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e2108155118","DOI":"10.1073\/pnas.2108155118","article-title":"Listeriolysin S: A Bacteriocin from Listeria monocytogenes That Induces Membrane Permeabilization in a Contact-Dependent Manner","volume":"118","author":"Lelek","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1080\/19490976.2017.1290759","article-title":"Listeriolysin S: A Bacteriocin from Epidemic Listeria monocytogenes Strains That Targets the Gut Microbiota","volume":"8","author":"Quereda","year":"2017","journal-title":"Gut Microbes"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/ng.3501","article-title":"Uncovering Listeria monocytogenes Hypervirulence by Harnessing Its Biodiversity","volume":"48","author":"Maury","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_12","first-page":"567","article-title":"Typing, Virulence and Biofilm Formation of the Food-Borne Listeria monocytogenes Carrying Pathogenicity Island 4","volume":"42","author":"Liu","year":"2020","journal-title":"Chin. J. Prev. Vet. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1128\/MMBR.00024-06","article-title":"How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria","volume":"70","author":"Deutscher","year":"2006","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_14","first-page":"36","article-title":"Construction of A Food-Borne Listeria monocytogenes Virulence Island 4 Gene Deletion Strain and Its in vitro Virulence Study","volume":"31","author":"Ruan","year":"2023","journal-title":"Chin. J. Anim. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e02097-22","DOI":"10.1128\/aem.02097-22","article-title":"Contrasting Genetic Diversity of Listeria Pathogenicity Islands 3 and 4 Harbored by Nonpathogenic Listeria spp.","volume":"89","author":"Lee","year":"2023","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","first-page":"2027","article-title":"Influence of Coexistence of LIPI-3 and LIPI-4 on Virulence of Pathogenic Listeria monocytogene","volume":"51","author":"Qian","year":"2024","journal-title":"China Anim. Husb. Vet. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1278860","DOI":"10.3389\/fmicb.2023.1278860","article-title":"Listeria monocytogenes, a Silent Foodborne Pathogen in Ecuador","volume":"14","author":"Freire","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref_18","first-page":"2380","article-title":"Detection and sequence analysis of LIPI-4 gene on food-borne Listeria monocytogenes","volume":"39","author":"Liu","year":"2019","journal-title":"Chin. J. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6891","DOI":"10.1128\/JB.00819-07","article-title":"Correlation between Growth Rates, EIIACrr Phosphorylation, and Intracellular Cyclic AMP Levels in Escherichia coli K-12","volume":"189","author":"Bettenbrock","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1099\/mic.0.070953-0","article-title":"Lipid Dependencies, Biogenesis and Cytoplasmic Micellar Forms of Integral Membrane Sugar Transport Proteins of the Bacterial Phosphotransferase System","volume":"159","author":"Aboulwafa","year":"2013","journal-title":"Microbiology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/MMBR.00001-14","article-title":"The Bacterial Phosphoenolpyruvate:Carbohydrate Phosphotransferase System: Regulation by Protein Phosphorylation and Phosphorylation-Dependent Protein-Protein Interactions","volume":"78","author":"Deutscher","year":"2014","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.micres.2019.04.002","article-title":"Understanding the Multifaceted Roles of the Phosphoenolpyruvate: Phosphotransferase System in Regulation of Salmonella Virulence Using a Mutant Defective in ptsI and crr Expression","volume":"223\u2013225","author":"Lim","year":"2019","journal-title":"Microbiol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e32385","DOI":"10.1371\/journal.pone.0032385","article-title":"Contribution of a Genomic Accessory Region Encoding a Putative Cellobiose Phosphotransferase System to Virulence of Streptococcus pneumoniae","volume":"7","author":"McAllister","year":"2012","journal-title":"PLoS ONE"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.3389\/fmicb.2017.01611","article-title":"LMOf2365_0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L. monocytogenes Strain F2365","volume":"8","author":"Liu","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"736340","DOI":"10.1016\/j.aquaculture.2021.736340","article-title":"The Influence of Enzyme EII of the Cellobiose-Phosphotransferase System on the Virulence of Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus)","volume":"535","author":"Xie","year":"2021","journal-title":"Aquaculture"},{"key":"ref_26","first-page":"e00213-18","article-title":"Cross Talk among Transporters of the Phosphoenolpyruvate-Dependent Phosphotransferase System in Bacillus subtilis","volume":"200","author":"Altenbuchner","year":"2018","journal-title":"J. Bacteriol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1128\/JB.02522-14","article-title":"Interaction with Enzyme IIBMpo (EIIBMpo) and Phosphorylation by Phosphorylated EIIBMpo Exert Antagonistic Effects on the Transcriptional Activator ManR of Listeria monocytogenes","volume":"197","author":"Ventroux","year":"2015","journal-title":"J. Bacteriol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/jfd.12907","article-title":"Virulence Regulation of Cel-EIIB Protein Mediated PTS System in Streptococcus agalactiae in Nile Tilapia","volume":"42","author":"Xu","year":"2019","journal-title":"J. Fish Dis."},{"key":"ref_29","first-page":"1360","article-title":"Analysis of Expression Patterns of Virulence-Related Genes Regulated by the Cel-EIIB Protein in Virulent and Attenuated Strains of Streptococcus agalactiae in Tilapia","volume":"27","author":"Su","year":"2020","journal-title":"J. Fish. Sci. China"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Liu, C., Qian, R., Shi, W., Kou, L., Wang, J., Ma, X., Ren, H., Gao, S., and Ren, J. (2024). EIIB Mutation Reduces the Pathogenicity of Listeria monocytogenes by Negatively Regulating Biofilm Formation Ability, Infective Capacity, and Virulence Gene Expression. Vet. Sci., 11.","DOI":"10.3390\/vetsci11070301"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Shi, W., Zhang, Q., Li, H., Du, D., Ma, X., Wang, J., Jiang, J., Liu, C., Kou, L., and Ren, J. (2024). Biofilm Formation, Motility, and Virulence of Values Represent the Mean Listeria monocytogenes Are Reduced by Deletion of the Gene Lmo0159, a Novel Listerial LPXTG Surface Protein. Microorganisms, 12.","DOI":"10.3390\/microorganisms12071354"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1128\/IAI.00849-15","article-title":"RNA Helicase Important for Listeria monocytogenes Hemolytic Activity and Virulence Factor Expression","volume":"84","author":"Netterling","year":"2016","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1884517","DOI":"10.1080\/19490976.2021.1884517","article-title":"YjbH Mediates the Oxidative Stress Response and Infection by Regulating SpxA1 and the Phosphoenolpyruvate-Carbohydrate Phosphotransferase System (PTS) in Listeria monocytogenes","volume":"13","author":"Cheng","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2543144","DOI":"10.1080\/21505594.2025.2543144","article-title":"Function Analysis of LIPI-4 in Listeria monocytogenes Reveals a Key Role of Flagella Formation in the Regulation of Virulence","volume":"16","author":"Liu","year":"2025","journal-title":"Virulence"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e88","DOI":"10.4142\/jvs.2020.21.e88","article-title":"Deletion of the Oligopeptide Transporter Lmo2193 Decreases the Virulence of Listeria monocytogenes","volume":"21","author":"Li","year":"2020","journal-title":"J. Vet. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e8","DOI":"10.4142\/jvs.21224","article-title":"Nucleomodulin BspJ as an Effector Promotes the Colonization of Brucella abortus in the Host","volume":"23","author":"Ma","year":"2021","journal-title":"J. Vet. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Kuhn, A. (2019). Carbohydrate Transport by Group Translocation: The Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System. Bacterial Cell Walls and Membranes, Springer International Publishing.","DOI":"10.1007\/978-3-030-18768-2"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1128\/JB.02042-12","article-title":"Comprehensive Mutational Analysis of Sucrose-Metabolizing Pathways in Streptococcus mutans Reveals Novel Roles for the Sucrose Phosphotransferase System Permease","volume":"195","author":"Zeng","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e01322-22","DOI":"10.1128\/spectrum.01322-22","article-title":"Development of a Glycerol-Inducible Expression System for High-Yield Heterologous Protein Production in Bacillus subtilis","volume":"10","author":"Han","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_40","first-page":"10","article-title":"Studies of the Listeria monocytogenes Cellobiose Transport Components and Their Impact on Virulence Gene Repression","volume":"29","author":"Cao","year":"2019","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.3389\/fmicb.2019.02698","article-title":"Biofilm Formation of Listeria monocytogenes Strains Under Food Processing Environments and Pan-Genome-Wide Association Study","volume":"10","author":"Lee","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"898","DOI":"10.3389\/fmicb.2018.00898","article-title":"Biofilms in the Food Industry: Health Aspects and Control Methods","volume":"9","author":"Villar","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jmii.2017.01.007","article-title":"Phosphoenolpyruvate Phosphotransferase System Components Positively Regulate Klebsiella Biofilm Formation","volume":"51","author":"Horng","year":"2018","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.resmic.2018.10.002","article-title":"The Phosphotransferase System Gene ptsH Plays an Important Role in MnSOD Production, Biofilm Formation, Swarming Motility, and Root Colonization in Bacillus cereus 905","volume":"170","author":"Gao","year":"2019","journal-title":"Res. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s43088-024-00504-x","article-title":"Estimating the Expression Levels of Genes Controlling Biofilm Formation and Evaluating the Effects of Different Conditions on Biofilm Formation and Secreted Aspartic Proteinase Activity in Candida albicans and Saccharomyces cerevisiae: A Comparative Study","volume":"13","author":"Sobieh","year":"2024","journal-title":"Beni-Suef Univ. J. Basic Appl. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1038\/cr.2015.129","article-title":"PslG, a Self-Produced Glycosyl Hydrolase, Triggers Biofilm Disassembly by Disrupting Exopolysaccharide Matrix","volume":"25","author":"Yu","year":"2015","journal-title":"Cell Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Huq, M., Wahid, S.U.H., and Istivan, T. (2024). Biofilm Formation in Campylobacter concisus: The Role of the luxS Gene. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010046"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1111\/mpp.13389","article-title":"degQ Associated with the degS\/degU Two-Component System Regulates Biofilm Formation, Antimicrobial Metabolite Production, and Biocontrol Activity in Bacillus velezensis DMW1","volume":"24","author":"Yu","year":"2023","journal-title":"Mol. Plant Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"109405","DOI":"10.1016\/j.foodres.2020.109405","article-title":"Influence of Different Factors on Biofilm Formation of Listeria monocytogenes and the Regulation of cheY Gene","volume":"137","author":"Fan","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e1015","DOI":"10.1002\/mbo3.1015","article-title":"Links between S-Adenosylmethionine and Agr-Based Quorum Sensing for Biofilm Development in Listeria monocytogenes EGD-e","volume":"9","author":"Lee","year":"2020","journal-title":"MicrobiologyOpen"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e1002153","DOI":"10.1371\/journal.ppat.1002153","article-title":"A Protein Thermometer Controls Temperature-Dependent Transcription of Flagellar Motility Genes in Listeria monocytogenes","volume":"7","author":"Kamp","year":"2011","journal-title":"PLoS Pathog."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.1462-5822.2008.01272.x","article-title":"Cell-to-Cell Signalling during Pathogenesis","volume":"11","author":"Parker","year":"2009","journal-title":"Cell. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1084\/jem.20110560","article-title":"Transcytosis of Listeria monocytogenes across the Intestinal Barrier upon Specific Targeting of Goblet Cell Accessible E-Cadherin","volume":"208","author":"Nikitas","year":"2011","journal-title":"J. Exp. Med."},{"key":"ref_54","first-page":"1179","article-title":"Biological Function Study of the Phosphotransferase System (PTS) Transporter Protein IIBman in Mediating Motility and Infection in Listeria monocytogenes","volume":"45","author":"Zhang","year":"2023","journal-title":"Chin. J. Prev. Vet. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e00858-18","DOI":"10.1128\/mBio.00858-18","article-title":"Removal of a Membrane Anchor Reveals the Opposing Regulatory Functions of Vibrio cholerae Glucose-Specific Enzyme IIA in Biofilms and the Mammalian Intestine","volume":"9","author":"Vijayakumar","year":"2018","journal-title":"mBio"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4504","DOI":"10.1111\/1462-2920.14740","article-title":"HprKXcc Is a Serine Kinase That Regulates Virulence in the Gram-Negative Phytopathogen Xanthomonas campestris","volume":"21","author":"Li","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"L252","DOI":"10.1152\/ajplung.00199.2023","article-title":"Fatty Acid Metabolism Promotes TRPV4 Activity in Lung Microvascular Endothelial Cells in Pulmonary Arterial Hypertension","volume":"326","author":"Philip","year":"2024","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.cytogfr.2023.05.001","article-title":"The Role of Extracellular Vesicles in Glycolytic and Lipid Metabolic Reprogramming of Cancer Cells: Consequences for Drug Resistance","volume":"73","year":"2023","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/s41586-024-07787-1","article-title":"Glycosphingolipid Synthesis Mediates Immune Evasion in KRAS-Driven Cancer","volume":"633","author":"Soula","year":"2024","journal-title":"Nature"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1038\/s41467-025-59747-6","article-title":"Mapping Pesticide-Induced Metabolic Alterations in Human Gut Bacteria","volume":"16","author":"Chen","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1038\/s41467-025-58533-8","article-title":"Maternal Gut Microbiota Influences Immune Activation at the Maternal-Fetal Interface Affecting Pregnancy Outcome","volume":"16","author":"Giugliano","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"962326","DOI":"10.3389\/fmicb.2022.962326","article-title":"Recombinant Listeria ivanovii Strain Expressing Listeriolysin O in Place of Ivanolysin O Might Be a Potential Antigen Carrier for Vaccine Construction","volume":"13","author":"Liang","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_63","first-page":"152","article-title":"Comparison of the Effects of ILO and LLO in Helping Listeria Adhere, Invade Cell and Intracellularly Multiply","volume":"50","author":"Liu","year":"2019","journal-title":"J. Sichuan Univ. Med. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.mib.2011.01.005","article-title":"Regulation of Listeria Virulence: PrfA Master and Commander","volume":"14","author":"Cain","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e13496","DOI":"10.1111\/mpp.13496","article-title":"A Cyclic Di-GMP-Binding Adaptor Protein Interacts with a N5-Glutamine Methyltransferase to Regulate the Pathogenesis in Xanthomonas citri subsp","volume":"25","author":"Shi","year":"2024","journal-title":"Citri. Mol. Plant Pathol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"115290","DOI":"10.1016\/j.celrep.2025.115290","article-title":"Structural Basis of Promiscuous Inhibition of Listeria Virulence Activator PrfA by Oligopeptides","volume":"44","author":"Hainzl","year":"2025","journal-title":"Cell Rep."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/mmi.14413","article-title":"Influence of the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System on Toxin Gene Expression and Virulence in Bacillus anthracis","volume":"113","author":"Bier","year":"2020","journal-title":"Mol. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"fnaa193","DOI":"10.1093\/femsle\/fnaa193","article-title":"Transcriptional Profiling of the L. monocytogenes PrfA Regulon Identifies Six Novel Putative PrfA-Regulated Genes","volume":"367","author":"Henderson","year":"2020","journal-title":"FEMS Microbiol. 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