{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:46:00Z","timestamp":1759452360761,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"key agricultural technology research project of Hubei province","award":["HBNYHXGG2024-3"],"award-info":[{"award-number":["HBNYHXGG2024-3"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The long-term application of traditional chemical herbicides has caused a significant escalation in herbicide resistance of barnyard grass (Echinochloa crus-galli). As an eco-friendly alternative, biological herbicides demonstrate substantial application potential. Acknowledging the growing herbicide resistance of E. crus-galli, this study aimed to screen target bacteria with inhibitory effects on the growth for bio-herbicide development. By using ungerminated E. crus-galli seeds as the screening substrate, a bacterial strain (BFYBC-8) with potent inhibitory activity was isolated and identified as Brucella pseudorignonensis. Pot experiments revealed that inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7% and root length by 85.1%, while markedly inhibiting biomass accumulation. Fluorescent labeling with green fluorescent protein (GFP) showed that BFYBC-8 successfully colonized the root intercellular spaces of E. crus-galli and extended continuously along the tissue matrix. Additionally, the strain\u2019s supernatant metabolic products exhibited exceptional thermostability: inhibitory activity against E. crus-galli was maintained after thermal treatment at 28 \u00b0C, 60 \u00b0C, 80 \u00b0C, and 100 \u00b0C. Crucially, the bacterium displayed no toxicity to agronomically important crops such as rice, wheat, and corn. This study highlights B. pseudorignonensis BFYBC-8 as a promising candidate for bioherbicide development and provides an important reference for applying seed-associated pathogenic bacteria in developing bioherbicides for sustainable weed management.<\/jats:p>","DOI":"10.3390\/microorganisms13102293","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:07:48Z","timestamp":1759417668000},"page":"2293","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Herbicidal Control Potential of the Endophytic Bacterium B. pseudorignonensis BFYBC-8 Isolated from E. crus-galli Seeds"],"prefix":"10.3390","volume":"13","author":[{"given":"Dashan","family":"Yang","sequence":"first","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanlong","family":"He","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingling","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Ke","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawei","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwei","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, H., Mu, D., Li, Y., Li, X., Yan, X., Li, K., Jiao, Y., Li, J., Lin, H., and Lin, W. (2024). Glutathione S-transferase activity facilitates rice tolerance to the b arnyard grass root exudate DIMBOA. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-04802-5"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rahaman, F., Juraimi, A.S., Rafii, M.Y., Uddin, M.K., Hassan, L., Chowdhury, A.K., and Bashar, H.M.K. (2021). Allelopathic effect of selected rice (Oryza sativa) varieties against barnyard grass (Echinochloa crus-gulli). Plants, 10.","DOI":"10.3390\/plants10102017"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"117","DOI":"10.15446\/acag.v69n2.81305","article-title":"var. mitis metabolism as resistance mechanism to imazapyr and imazapic","volume":"69","author":"Andres","year":"2020","journal-title":"Acta Agron\u00f3mica"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1002\/agj2.20101","article-title":"Biosolids and biochar application effects on bahiagrass herbage accumu lation and nutritive value","volume":"112","author":"Lu","year":"2020","journal-title":"Agron. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1007\/s11033-019-05205-8","article-title":"Rice momilactone gene cluster: Transcriptional response to barnyard grass (Echinochloa crus-galli)","volume":"47","author":"Pan","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.ecoenv.2018.04.018","article-title":"Bioherbicides: Current knowledge on weed control mechanism","volume":"158","author":"Radhakrishnan","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Treiber, L., Pezolt, C., Zeng, H., Schrey, H., Jungwirth, S., Shekhar, A., Stadler, M., Bilitewski, U., Erb-Brinkmann, M., and Schobert, R. (2021). Dual agents: Fungal macrocidins and synthetic analogues with herbicida l and antibiofilm activities. Antibiotics, 10.","DOI":"10.3390\/antibiotics10081022"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1039\/C5NP00081E","article-title":"Fungal phytotoxins with potential herbicidal activity: Chemical and bi ological characterization","volume":"32","author":"Cimmino","year":"2015","journal-title":"Nat. Prod. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1080\/21655979.2021.1877413","article-title":"Bio-herbicidal potential of wheat rhizosphere bacteria on Avena fatua L. grass","volume":"12","author":"Li","year":"2021","journal-title":"Bioengineered"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1017\/wsc.2019.24","article-title":"Genetic potential of rice under alternate-wetting-and-drying irrigation management for barnyard grass (Echinochloa crus-galli) suppres sion and grain yield production","volume":"67","author":"Gealy","year":"2019","journal-title":"Weed Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.biocontrol.2005.07.009","article-title":"Ecological, practical, and political inputs into selection of weed tar gets: What makes a good biological control target?","volume":"35","author":"Charudattan","year":"2005","journal-title":"Biol. Control"},{"key":"ref_12","first-page":"1","article-title":"Effect of fertilizer on palisadegrass seeds pathogens","volume":"34","author":"Avelino","year":"2019","journal-title":"J. Exp. Agric. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.rama.2019.10.007","article-title":"Effect of the Bioherbicide Pseudomonas fluorescens D7 on downy brome (Bromus tectorum)","volume":"73","author":"Tekiela","year":"2020","journal-title":"Rangel. Ecol. Manag."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/09583157.2014.966650","article-title":"Bioherbicidal potential of Xanthomonas campestris for controlling Conyza canadensis","volume":"25","author":"Boyette","year":"2015","journal-title":"Biocontrol Sci. Technol."},{"key":"ref_15","first-page":"95","article-title":"Cross-genera transferability of rice and finger millet genomic SSRs to barnyard millet (Echinochloa spp.)","volume":"8","author":"Sood","year":"2018","journal-title":"3 Biotech"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3923\/pjbs.2016.82.88","article-title":"Competitive and allelopathic effects of wild rice accessions (Oryza longistaminata) at different growth stages","volume":"19","author":"Shen","year":"2016","journal-title":"Pak. J. Biol. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.fbio.2015.12.007","article-title":"Analysing the effect of germination on phenolics, dietary fibres, mine rals and \u03b3-amino butyric acid contents of barnyard millet (Echinochloa frumentaceae)","volume":"13","author":"Sharma","year":"2016","journal-title":"Food Biosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"108135","DOI":"10.1016\/j.envint.2023.108135","article-title":"Hotspots of soil pollution: Possible risks of glyphosate and aminometh ylphosphonic acid on terrestrial ecosystems and human health","volume":"179","author":"Ferreira","year":"2023","journal-title":"Environ Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2259526","DOI":"10.1080\/21655979.2023.2259526","article-title":"Health effects of herbicides and its current removal strategies","volume":"14","author":"Wahab","year":"2023","journal-title":"Bioengineered"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Singh, P., and Prasad, S.M. (2020). Impact of Herbicide Use on Soil Microorganisms and Their Diversity. Plant Responses to Soil Pollution, Springer.","DOI":"10.1007\/978-981-15-4964-9_11"},{"key":"ref_21","first-page":"566","article-title":"Effect of processing on the characteristics changes in barnyard and foxtail millet","volume":"7","author":"Nazni","year":"2016","journal-title":"J. Food Process. Amp Technol."},{"key":"ref_22","first-page":"129","article-title":"Evaluation of allelopathic effects of iranian rice cultivars (Oryza sativa L.) on growth factors of barnyard grass (Echinochloa crus-galli L.)","volume":"7","author":"Sanavy","year":"2015","journal-title":"J. Agric. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1093\/bioinformatics\/btq224","article-title":"Parallelization of the MAFFT multiple sequence alignment program","volume":"26","author":"Katoh","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian inference of phylogenetic trees","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/s13568-023-01618-8","article-title":"Klebsiella michiganensis: A nitrogen-fixing endohyphal bacterium from Ustilago maydis","volume":"13","author":"Liang","year":"2023","journal-title":"AMB Express"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ejsobi.2013.01.006","article-title":"The response of allelopathic rice growth and microbial feedback to barnyard grass infestation in a paddy field experiment","volume":"56","author":"Wang","year":"2013","journal-title":"Eur. J. Soil Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/MMBR.00021-19","article-title":"Uncovering the hidden credentials of Brucella virulence","volume":"85","author":"Roop","year":"2021","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Su\u00e1rez-Esquivel, M., Chaves-Olarte, E., Moreno, E., and Guzm\u00e1n-Verri, C. (2020). Brucella genomics: Macro and micro evolution. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207749"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.cropro.2004.09.009","article-title":"Evaluation of allelopathic potential among rice (Oryza sativa L.) germ plasm for control of Echinochloa crus-galli P. Beauv in the field","volume":"24","author":"Ahn","year":"2004","journal-title":"Crop Prot."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1139\/w11-109","article-title":"Colonization and bioherbicidal activity on green foxtail by Pseudomonas fluorescens BRG100 in a pesta formulation","volume":"58","author":"Caldwell","year":"2012","journal-title":"Can. J. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.biocontrol.2016.08.004","article-title":"Mycorrhizal composition can predict foliar pathogen colonization in soybean","volume":"103","author":"Malik","year":"2016","journal-title":"Biol. Control"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1017\/S0890037X00040549","article-title":"Controlling weeds with phytopathogenic bacteria","volume":"10","author":"Johnson","year":"1996","journal-title":"Weed Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3786","DOI":"10.1002\/ps.8081","article-title":"Effects of Bipolaris yamadae strain HXDC-1-2 as a bioherbicide against Echinochloa crus-gall in rice and dry fields","volume":"80","author":"Tan","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"113532","DOI":"10.1016\/j.phytochem.2022.113532","article-title":"Application of cinnamic acid in the structural modification of natural products: A review","volume":"206","author":"Deng","year":"2022","journal-title":"Phytochemistry"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1080\/10286020.2020.1839432","article-title":"1,8-Cineole: A review of source, biological activities, and application","volume":"23","author":"Cai","year":"2020","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.3390\/ijms15011441","article-title":"Secondary Plant Products Causing Photosensitization in Grazing Herbivo res: Their Structure, Activity and Regulation","volume":"15","author":"Quinn","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12012","DOI":"10.1002\/chem.201601952","article-title":"Betaine and carnitine derivatives as herbicidal ionic liquids","volume":"22","author":"Pernak","year":"2016","journal-title":"Chem. A Eur. J."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/10\/2293\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:23:54Z","timestamp":1759418634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/10\/2293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,2]]},"references-count":38,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["microorganisms13102293"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13102293","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,2]]}}}