{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T17:28:54Z","timestamp":1777829334523,"version":"3.51.4"},"reference-count":46,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/04023-0"],"award-info":[{"award-number":["2015\/04023-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Universidade Estadual Paulista J\u00falio de Mesquita Filho","award":["EDITAL 09\/2025-PROPG"],"award-info":[{"award-number":["EDITAL 09\/2025-PROPG"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Sporotrichosis is a globally distributed mycosis caused by thermally dimorphic fungi of the Sporothrix schenckii species complex. In Brazil, sporotrichosis is considered endemic and is usually acquired through zoonotic transmission from infected cats. The clinical manifestations may be cutaneous, lymphocutaneous, or systemic, the latter being more commonly observed in immunosuppressed patients. The limited effectiveness of antifungal treatments against this mycosis, particularly in immunocompromised individuals, has led to the search for more effective and safer therapies. Based on several studies demonstrating the efficient use of dendritic cells as tools for the development of antifungal vaccines, this work aimed to evaluate the protective capacity of bone marrow-derived dendritic cells (BMDCs) activated with cell wall proteins of S. schenckii (SsCWP) in mice infected with S. schenckii sensu stricto. BMDCs were stimulated with SsCWP and analyzed for the surface expression of costimulatory molecules as well as proinflammatory cytokine secretion. Subsequently, mice were vaccinated once or twice to assess immunogenicity, and finally, the therapeutic effect of BMDCs on S. schenckii infection was evaluated. Our results show that SsCWP was able to activate BMDCs. Immunization of healthy mice with SsCWP-stimulated BMDCs induced a balanced Th1\/Th17-based immune response. Vaccination of mice previously infected with S. schenckii induced a mixed Th1\/Th17 response and reduced fungal burden in the spleen. Overall, these findings demonstrate that therapeutic vaccination with SsCWP-stimulated BMDCs improves fungal control, supporting the notion that dendritic cells represent a promising therapeutic strategy against sporotrichosis.<\/jats:p>","DOI":"10.3390\/microorganisms13102351","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T10:37:14Z","timestamp":1760438234000},"page":"2351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dendritic Cell-Based Therapeutic Immunization Induces Th1\/Th17 Responses and Reduces Fungal Burden in Experimental Sporotrichosis"],"prefix":"10.3390","volume":"13","author":[{"given":"Juliana Aparecida","family":"Jellmayer","sequence":"first","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Fernandes de","family":"Deus","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus Ricardo","family":"Curti Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Souza Ferreira","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine Alessandra","family":"Manente","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Silva","family":"Pinho Caetano","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3958-3218","authenticated-orcid":false,"given":"Fernanda Luiza","family":"Piccineli","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1462-440X","authenticated-orcid":false,"given":"Thais","family":"Zamber\u00e7o dos Reis Genari","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5836-4061","authenticated-orcid":false,"given":"Beatriz da Cunha","family":"Sa\u00e7aki","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarcila","family":"Pavicic Catalan de Oliveira Campos","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2678-0431","authenticated-orcid":false,"given":"Deivys Leandro","family":"Portuondo","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Batista-Duharte","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"},{"name":"Department of Cell Biology\u2014Physiology and Immunology, University of Cordoba, 14004 C\u00f3rdoba, Spain"},{"name":"Immunology and Allergy Group (GC01)\u2014Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital, 14004 C\u00f3rdoba, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0084-3468","authenticated-orcid":false,"given":"Iracilda Zeppone","family":"Carlos","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis\u2014School of Pharmaceutical Sciences, S\u00e3o Paulo State University\u2014UNESP, Araraquara 14800-901, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/imr.12280","article-title":"The Interplay Between Inflammasome Activation and Antifungal Host Defense","volume":"265","author":"Joosten","year":"2015","journal-title":"Immunol. 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