{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:09:49Z","timestamp":1787836189917,"version":"build-2784847793"},"reference-count":64,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research and Development Fund of Zhejiang A&F University","award":["2023LFR006"],"award-info":[{"award-number":["2023LFR006"]}]},{"name":"Open Project of Key Laboratory of Animal Molecular Nutrition (Zhejiang University), Ministry of Education","award":["KLMAN202307"],"award-info":[{"award-number":["KLMAN202307"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The gut microbiota plays a vital role in regulating the host\u2019s physiological functions, including metabolism and immunity. The microbial composition and metabolism are modulated by multiple factors; host sex is an important yet under-explored determinant. To investigate the sex-dependent differences in the gut microbiota within the small and large intestine, sixteen somatic mature Jiangshan black pigs (eight males and eight females) were analyzed. The ileal and colonic microbial community and metabolites were profiled using 16S rRNA gene high-throughput sequencing and gas chromatography. Distinct sex-related discrepancies were observed in both the microbial composition and metabolism of the ileum and colon. In the ileum, compared with the male group, the female group exhibited higher abundances of Unclassified Chloroplast and Pseudomonas but a lower abundance of Romboutsia (adjusted p &lt; 0.05). Functional prediction indicated enrichment in amino acid metabolism pathway in females, with more copy numbers of genes encoding key enzymes for propionate (mmdA) generation and elevated valerate levels (p &lt; 0.05). In the colon, compared with the male group, the female group showed higher abundances of Streptococcus, Phascolarctobacterium, and Prevotella spp. and lower abundances of Eubacterium coprostano-ligenes group, Blautia, Christensenellaceae R-7 group, and Ruminococcus (adjusted p &lt; 0.05). Additionally, the female group had more copies of genes mmdA and LcdA (associated with lactate production), along with higher concentrations of propionate and lactate (p &lt; 0.05). Correlation analysis between microbial metabolites and sex-biased bacteria further revealed that the SCFA concentration positively correlated with Prevotella spp. and negatively correlated with Romboutsia, Christensenellaceae R-7 group, and Blautia. Collectively, these findings highlight the pronounced sex-dependent discrepancies in the microbial composition and metabolism within the small and large intestines of Jiangshan black pigs, providing new insights for precisely modulating the microbiota community and metabolism in pigs according to sex.<\/jats:p>","DOI":"10.3390\/microorganisms13112551","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T14:55:15Z","timestamp":1762527315000},"page":"2551","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sex-Specific Differences in Gut Microbial Composition and Metabolism of Jiangshan Black Pigs"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-8130","authenticated-orcid":false,"given":"Yanan","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"},{"name":"The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5282-893X","authenticated-orcid":false,"given":"Dan","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8211-9691","authenticated-orcid":false,"given":"Haifeng","family":"Wang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangchen","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1111\/j.1462-2920.2007.01281.x","article-title":"Interactions and competition within the microbial community of the human colon: Links between diet and health","volume":"9","author":"Flint","year":"2007","journal-title":"Environ. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nrgastro.2012.156","article-title":"The role of the gut microbiota in nutrition and health","volume":"9","author":"Flint","year":"2012","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1146\/annurev-immunol-070119-115104","article-title":"Interaction Between the Microbiota, Epithelia, and Immune Cells in the Intestine","volume":"38","author":"Kayama","year":"2020","journal-title":"Annu. Rev. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1038\/ismej.2014.12","article-title":"Bacteria, phages and pigs: The effects of in-feed antibiotics on the microbiome at different gut locations","volume":"8","author":"Looft","year":"2014","journal-title":"ISME J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1111\/j.1365-2672.2007.03322.x","article-title":"Understanding the effects of diet on bacterial metabolism in the large intestine","volume":"102","author":"Louis","year":"2007","journal-title":"J. Appl. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2490207","DOI":"10.1080\/19490976.2025.2490207","article-title":"Gut microbiota and epigenetic inheritance: Implications for the development of IBD","volume":"17","author":"Guo","year":"2025","journal-title":"Gut Microbes"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Valeri, F., and Endres, K. (2021). How biological sex of the host shapes its gut microbiota. Front. Neuroendocrinol., 61.","DOI":"10.1016\/j.yfrne.2021.100912"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"314","DOI":"10.5056\/jnm20208","article-title":"Roles of Sex Hormones and Gender in the Gut Microbiota","volume":"27","author":"Yoon","year":"2021","journal-title":"J. Neurogastroenterol. Motil."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1080\/19490976.2018.1528822","article-title":"Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles","volume":"10","author":"Sinha","year":"2019","journal-title":"Gut Microbes"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00535-018-1488-5","article-title":"Differences in gut microbiota associated with age, sex, and stool consistency in healthy Japanese subjects","volume":"54","author":"Takagi","year":"2019","journal-title":"J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Elderman, M., Hugenholtz, F., Belzer, C., Boekschoten, M., van Beek, A., de Haan, B., Savelkoul, H., de Vos, P., and Faas, M. (2018). Sex and strain dependent differences in mucosal immunology and microbiota composition in mice. Biol. Sex Differ., 9.","DOI":"10.1186\/s13293-018-0186-6"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1126\/science.1233521","article-title":"Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity","volume":"339","author":"Markle","year":"2013","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xiao, L., Estell\u00e9, J., Kiilerich, P., Ramayo-Caldas, Y., Xia, Z., Feng, Q., Liang, S., Pedersen, A.\u00d8., Kjeldsen, N.J., and Liu, C. (2016). A reference gene catalogue of the pig gut microbiome. Nat. Microbiol., 1.","DOI":"10.1038\/nmicrobiol.2016.161"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"He, M., Gao, J., Wu, J., Zhou, Y., Fu, H., Ke, S., Yang, H., Chen, C., and Huang, L. (2019). Host Gender and Androgen Levels Regulate Gut Bacterial Taxa in Pigs Leading to Sex-Biased Serum Metabolite Profiles. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.01359"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Santos-Marcos, J.A., Mora-Ortiz, M., Tena-Sempere, M., Lopez-Miranda, J., and Camargo, A. (2023). Interaction between gut microbiota and sex hormones and their relation to sexual dimorphism in metabolic diseases. Biol. Sex Differ., 14.","DOI":"10.1186\/s13293-023-00490-2"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Baars, A., Oosting, A., Lohuis, M., Koehorst, M., El Aidy, S., Hugenholtz, F., Smidt, H., Mischke, M., Boekschoten, M.V., and Verkade, H.J. (2018). Sex differences in lipid metabolism are affected by presence of the gut microbiota. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-31695-w"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sisk-Hackworth, L., Brown, J., Sau, L., Levine, A.A., Tam, L.Y.I., Ramesh, A., Shah, R.S., Kelley-Thackray, E.T., Wang, S., and Nguyen, A. (2023). Genetic hypogonadal mouse model reveals niche-specific influence of reproductive axis and sex on intestinal microbial communities. Biol. Sex Differ., 14.","DOI":"10.1186\/s13293-023-00564-1"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00048-19","DOI":"10.1128\/mSystems.00048-19","article-title":"Antibiotic Exposure Has Sex-Dependent Effects on the Gut Microbiota and Metabolism of Short-Chain Fatty Acids and Amino Acids in Mice","volume":"4","author":"Gao","year":"2019","journal-title":"Msystems"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mu, C., Yang, Y., Su, Y., Zoetendal, E.G., and Zhu, W. (2017). Differences in Microbiota Membership along the Gastrointestinal Tract of Piglets and Their Differential Alterations Following an Early-Life Antibiotic Intervention. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00797"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhao, W., Wang, Y., Liu, S., Huang, J., Zhai, Z., He, C., Ding, J., Wang, J., Wang, H., and Fan, W. (2015). The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0117441"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nrmicro3050","article-title":"The abundance and variety of carbohydrate-active enzymes in the human gut microbiota","volume":"11","author":"Armougom","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1007\/s00726-014-1807-y","article-title":"Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs","volume":"46","author":"Yang","year":"2014","journal-title":"Amino Acids"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/sj.embor.7400731","article-title":"The gut flora as a forgotten organ","volume":"7","author":"Shanahan","year":"2006","journal-title":"EMBO Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1007\/s00726-010-0556-9","article-title":"Utilization of amino acids by bacteria from the pig small intestine","volume":"39","author":"Dai","year":"2010","journal-title":"Amino Acids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.3168\/jds.2020-19118","article-title":"Gene function adjustment for carbohydrate metabolism and enrichment of rumen microbiota with antibiotic resistance genes during subacute rumen acidosis induced by a high-grain diet in lactating dairy cows","volume":"104","author":"Mu","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W.S., and Huttenhower, C. (2011). Metagenomic biomarker discovery and explanation. Genome Biol., 12.","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1038\/nbt.2676","article-title":"Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences","volume":"31","author":"Langille","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"D109","DOI":"10.1093\/nar\/gkr988","article-title":"KEGG for integration and interpretation of large-scale molecular data sets","volume":"40","author":"Kanehisa","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.anaerobe.2017.06.003","article-title":"Temporal microbiota changes of high-protein diet intake in a rat model","volume":"47","author":"Mu","year":"2017","journal-title":"Anaerobe"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4605","DOI":"10.1128\/AEM.66.11.4605-4614.2000","article-title":"Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5\u2032-nuclease assays","volume":"66","author":"Suzuki","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/j.1472-765X.2008.02408.x","article-title":"Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs","volume":"47","author":"Guo","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1111\/j.1365-2672.2004.02409.x","article-title":"Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR","volume":"97","author":"Rinttila","year":"2004","journal-title":"J. Appl. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1128\/AEM.67.6.2578-2585.2001","article-title":"Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis","volume":"67","author":"Walter","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/ismej.2010.118","article-title":"Dominant and diet-responsive groups of bacteria within the human colonic microbiota","volume":"5","author":"Walker","year":"2011","journal-title":"ISME J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/ismej.2017.196","article-title":"Specific substrate-driven changes in human faecal microbiota composition contrast with functional redundancy in short-chain fatty acid production","volume":"12","author":"Reichardt","year":"2018","journal-title":"ISME J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1128\/aem.62.4.1242-1247.1996","article-title":"PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples","volume":"62","author":"Wang","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.anaerobe.2017.08.013","article-title":"Preferential isolation of Megasphaera elsdenii from pig feces","volume":"48","author":"Kajihara","year":"2017","journal-title":"Anaerobe"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.aninu.2022.08.005","article-title":"Dietary citrus pectin drives more ileal microbial protein metabolism and stronger fecal carbohydrate fermentation over fructo-oligosaccharide in growing pigs","volume":"11","author":"Zhang","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1111\/1751-7915.13282","article-title":"Caecal infusion of the short-chain fatty acid propionate affects the microbiota and expression of inflammatory cytokines in the colon in a fistula pig model","volume":"11","author":"Zhang","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1093\/jas\/sky086","article-title":"Effects of dietary protein sources and nisin on rumen fermentation, nutrient digestion, plasma metabolites, nitrogen utilization, and growth performance in growing lambs","volume":"96","author":"Shen","year":"2018","journal-title":"J. Anim. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.3168\/jds.S0022-0302(82)82477-6","article-title":"Simple determination of microbial protein in rumen liquor","volume":"65","author":"Makkar","year":"1982","journal-title":"J. Dairy Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5773","DOI":"10.1038\/s41467-020-19585-0","article-title":"Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut","volume":"11","author":"Michalak","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Han, Y., Liu, X., Jia, Q., Xu, J., Shi, J., Li, X., Xie, G., Zhao, X., and He, K. (2024). Longitudinal multi-omics analysis uncovers the altered landscape of gut microbiota and plasma metabolome in response to high altitude. Microbiome, 12.","DOI":"10.1186\/s40168-024-01781-5"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gao, X., Zhang, M., Xue, J., Huang, J., Zhuang, R., Zhou, X., Zhang, H., Fu, Q., and Hao, Y. (2018). Body Mass Index Differences in the Gut Microbiota Are Gender Specific. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01250"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Han, G.G., Lee, J.-Y., Jin, G.-D., Park, J., Choi, Y.H., Kang, S.-K., Chae, B.J., Kim, E.B., and Choi, Y.-J. (2018). Tracing of the fecal microbiota of commercial pigs at five growth stages from birth to shipment. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-24508-7"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1080\/19490976.2016.1203502","article-title":"Sex differences and hormonal effects on gut microbiota composition in mice","volume":"7","author":"Org","year":"2016","journal-title":"Gut Microbes"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1007\/s13213-015-1079-0","article-title":"How host gender affects the bacterial community in pig feces and its correlation to skatole production","volume":"65","author":"Zhou","year":"2015","journal-title":"Ann. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3654","DOI":"10.1038\/ncomms4654","article-title":"Gut microbiome of the Hadza hunter-gatherers","volume":"5","author":"Schnorr","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_52","first-page":"2313","article-title":"Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity","volume":"17","author":"Yang","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.phrs.2012.11.005","article-title":"Intestinal luminal nitrogen metabolism: Role of the gut microbiota and consequences for the host","volume":"68","author":"Davila","year":"2013","journal-title":"Pharmacol. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1802866","DOI":"10.1080\/19490976.2020.1802866","article-title":"Gut microbes from the phylogenetically diverse genus Eubacterium and their various contributions to gut health","volume":"12","author":"Mukherjee","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s12275-018-8024-4","article-title":"The Ruminococci: Key symbionts of the gut ecosystem","volume":"56","author":"Suen","year":"2018","journal-title":"J. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Crost, E.H., Coletto, E., Bell, A., and Juge, N. (2023). Ruminococcus gnavus: Friend or foe for human health. FEMS Microbiol. Rev., 47.","DOI":"10.1093\/femsre\/fuad014"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Mancabelli, L., Milani, C., Lugli, G.A., Turroni, F., Cocconi, D., van Sinderen, D., and Ventura, M. (2017). Identification of universal gut microbial biomarkers of common human intestinal diseases by meta-analysis. FEMS Microbiol. Ecol., 93.","DOI":"10.1093\/femsec\/fix153"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Waters, J.L., and Ley, R.E. (2019). The human gut bacteria Christensenellaceae are widespread, heritable, and associated with health. BMC Biol., 17.","DOI":"10.1186\/s12915-019-0699-4"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1017\/S0029665114001463","article-title":"Links between diet, gut microbiota composition and gut metabolism","volume":"74","author":"Flint","year":"2015","journal-title":"Proc. Nutr. Soc."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.tim.2014.03.001","article-title":"Phylogeny, culturing, and metagenomics of the human gut microbiota","volume":"22","author":"Walker","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Bai, Y., Zhou, X., Li, N., Zhao, J., Ye, H., Zhang, S., Yang, H., Pi, Y., Tao, S., and Han, D. (2021). In Vitro Fermentation Characteristics and Fiber-Degrading Enzyme Kinetics of Cellulose, Arabinoxylan, beta-Glucan and Glucomannan by Pig Fecal Microbiota. Microorganisms, 9.","DOI":"10.3390\/microorganisms9051071"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e2000250","DOI":"10.1002\/mnfr.202000250","article-title":"Stimulation of Gastric Transit Function Driven by Hydrolyzed Casein Increases Small Intestinal Carbohydrate Availability and Its Microbial Metabolism","volume":"64","author":"Shen","year":"2020","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"8564","DOI":"10.1128\/AEM.02443-12","article-title":"Lactate and acrylate metabolism by Megasphaera elsdenii under batch and steady-state conditions","volume":"78","author":"Prabhu","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1038\/ismej.2014.14","article-title":"Phylogenetic distribution of three pathways for propionate production within the human gut microbiota","volume":"8","author":"Reichardt","year":"2014","journal-title":"ISME J."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/11\/2551\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T15:12:04Z","timestamp":1762528324000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/11\/2551"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,7]]},"references-count":64,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["microorganisms13112551"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13112551","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,7]]}}}