{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T19:21:35Z","timestamp":1780773695985,"version":"3.54.1"},"reference-count":61,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022QC118"],"award-info":[{"award-number":["ZR2022QC118"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202810"],"award-info":[{"award-number":["32202810"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Multidrug-resistant (MDR) Streptococcus suis (S. suis) is a zoonotic pathogen capable of infecting pigs across all age groups, leading to conditions such as meningitis, arthritis, and endocarditis. In humans, infections can result in septic arthritis, meningitis, necrotizing fasciitis, and septicemia, which may be fatal. The absence of a complete genome sequence hinders comprehensive bioinformatic studies of MDR S. suis derived from pigs. In this study, we present the whole-genome sequence of MDR S. suis serotype 2 ST01 isolated from joint fluid samples obtained from pigs. Whole-genome analysis revealed that the ST01 chromosome carries 19 antibiotic resistance genes that confer resistance to major classes of antibiotic including aminoglycosides, tetracyclines, fluoroquinolones, lincosamides, polypeptide, and nitrofurans. Additionally, it contains 15 virulence factors associated with immune modulation, bacterial adherence, and stress survival. Whole-genome analysis identified 84 horizontal gene transfer elements in ST01 (comprising 28 genomic islands, 52 transposons, and 4 prophages), alongside mutations resulting in reduced virulence (302 instances) and loss of pathogenicity (34 instances). Furthermore, 18 antibiotic targets along with 21 lethal mutations were identified as potential targets for preventing, controlling, and treating infection caused by MDR S. suis serotype 2 ST01. In vivo infection experiments demonstrated that intraperitoneal inoculation with ST01 resulted in mortality among Kunming mice, with a median lethal dose (LD50) of 5.62 \u00d7 109 CFU\/mL. Histopathological analysis revealed varying degrees of lesions in the infected organs of the mice. This study thus provides valuable insights into strategies aimed at combating S. suis infections and their transmission within swine populations.<\/jats:p>","DOI":"10.3390\/microorganisms13112552","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T14:55:15Z","timestamp":1762527315000},"page":"2552","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of Antimicrobial Resistance and Virulence Factors in Multidrug-Resistant Streptococcus suis Serotype 2 Isolates Using Whole-Genome Sequencing"],"prefix":"10.3390","volume":"13","author":[{"given":"Lingling","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minglu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Sheng","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lumin","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yike","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock and Poultry Multi-omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinglin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.21775\/cimb.032.473","article-title":"Recent proceedings on prevalence and pathogenesis of Streptococcus suis","volume":"32","author":"Tan","year":"2019","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Segura, M. (2020). Streptococcus suis research: Progress and challenges. Pathogens, 9.","DOI":"10.3390\/pathogens9090707"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.tim.2009.12.003","article-title":"Uncovering newly emerging variants of Streptococcus suis, an important zoonotic agent","volume":"18","author":"Feng","year":"2010","journal-title":"Trends Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2339946","DOI":"10.1080\/22221751.2024.2339946","article-title":"Investigation of genomic and pathogenicity characteristics of Streptococcus suis ST1 human strains from Guangxi Zhuang Autonomous Region (GX) between 2005 and 2020 in China","volume":"13","author":"Kang","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.3201\/eid1208.060232","article-title":"Streptococcus suis sequence type 7 outbreak, Sichuan, China","volume":"12","author":"Ye","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0378-1135(00)00250-9","article-title":"The pathogenesis of the meningitis caused by Streptococcus suis: The unresolved questions","volume":"76","author":"Gottschalk","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"259","DOI":"10.2217\/fmb.11.149","article-title":"Virulence factors involved in the pathogenesis of the infection caused by the swine pathogen and zoonotic agent Streptococcus suis","volume":"7","author":"Fittipaldi","year":"2012","journal-title":"Future Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Segura, M., Aragon, V., Brockmeier, S.L., Gebhart, C., Greeff, A., Kerdsin, A., O\u2019Dea, M.A., Okura, M., Sal\u00e9ry, M., and Schultsz, C. (2020). Update on Streptococcus suis research and prevention in the Era of antimicrobial restriction: 4th International Workshop on S. suis. Pathogens, 9.","DOI":"10.3390\/pathogens9050374"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Tang, J., Wang, C., Feng, Y., Yang, W., Song, H., Chen, Z., Yu, H., Pan, X., Zhou, X., and Wang, H. (2006). Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2. PLoS Med., 3.","DOI":"10.1371\/journal.pmed.0030377"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/tbed.12157","article-title":"Virulence genes and genetic diversity of Streptococcus suis serotype 2 isolates from Thailand","volume":"60","author":"Maneerat","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6030","DOI":"10.1016\/j.vaccine.2017.03.033","article-title":"A novel live attenuated anthrax spore vaccine based on an acapsular Bacillus anthracis Sterne strain with mutations in the htrA, lef and cya genes","volume":"35","author":"Chitlaru","year":"2017","journal-title":"Vaccine"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8649","DOI":"10.1007\/s00253-020-10873-9","article-title":"Antibiotic resistance related to biofilm formation in Streptococcus suis","volume":"104","author":"Yi","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Palmieri, C., Varaldo, P.E., and Facinelli, B. (2011). Streptococcus suis, an emerging drug-resistant animal and human pathogen. Front. Microbiol., 2.","DOI":"10.3389\/fmicb.2011.00235"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bonifait, L., Dominguez-Punaro, M.D.L.C., Vaillancourt, K., Bart, C., Slater, J., Frenette, M., Gottschalk, M., and Grenier, D. (2010). The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis. BMC Microbiol., 10.","DOI":"10.1186\/1471-2180-10-42"},{"key":"ref_15","first-page":"107","article-title":"Streptococcus suis and S. parasuis in the Philippines: Biochemical, molecular, and antimicrobial resistance characterization of the first isolates from local swine","volume":"13","author":"Sedano","year":"2020","journal-title":"Philipp. Sci. Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109149","DOI":"10.1016\/j.vetmic.2021.109149","article-title":"The population structure, antimi-crobial resistance, and pathogenicity of Streptococcus suis cps31","volume":"259","author":"Wang","year":"2021","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1080\/22221751.2021.1988725","article-title":"Genomic and pathogenic investigations of Streptococcus suis serotype 7 population derivedfrom a human patient and pigs","volume":"10","author":"Liang","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Scherrer, S., Biggel, M., Schneeberger, M., Cernela, N., Rademacher, F., Schmitt, S., and Stephan, R. (2024). Genetic diversity and antimicrobial susceptibility of Streptococcus suis from diseased Swiss pigs collected between 2019\u20132022. Vet. Microbiol., 293.","DOI":"10.1016\/j.vetmic.2024.110084"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Uru\u00e9n, C., Gimeno, J., Sanz, M., Fraile, L., Mar\u00edn, C.M., and Arenas, J. (2024). Invasive Streptococcus suis isolated in Spain contain a highly promiscuous and dynamic resistome. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1329632"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5779652","DOI":"10.1155\/tbed\/5779652","article-title":"Streptococcus suis Serotype 14: A Nonnegligible Zoonotic Population","volume":"2025","author":"Wu","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Du, F., Lv, X., Duan, D., Wang, L., and Huang, J. (2019). Characterization of a Linezolid- and Vancomycin-Resistant Streptococcus suis Isolate That Harbors optrA and vanG Operons. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02026"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Negare, R., and Suzana, K.S. (2019). Mechanisms of Action for Antimicrobial Peptides with Antibacterial and Antibiofilm Functions. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02866"},{"key":"ref_23","unstructured":"(2022). Microbiology of the Food Chain-Whole Genome Sequencing for Typing and Genomic Characterization of Bacteria-General Requirements and Guidance (Standard No. ISO\/IEC 23418:2022). Available online: https:\/\/www.iso.org\/standard\/75509.html."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/nyas.13310","article-title":"Challenges and opportunities for whole-genome sequencing\u2013based surveillance of antibiotic resistance","volume":"1388","author":"Anita","year":"2017","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"477","DOI":"10.4161\/viru.28595","article-title":"Streptococcus suis infection: An emerging\/reemerging challenge of bacterial infectious diseases?","volume":"5","author":"Feng","year":"2014","journal-title":"Virulence"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1089\/vbz.2018.2399","article-title":"Streptococcus suis Meningitis: Epidemiology, Clinical Presentation and Treatment","volume":"19","author":"Hlebowicz","year":"2019","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_27","unstructured":"(2017). Streptococcus Suis Inspection Operating Procedures (Standard No. DB34\/T 2996-2017)."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.medmal.2017.10.001","article-title":"Understanding the virulence of Streptococcus suis: A veterinary, medical, and economic challenge","volume":"48","author":"Haas","year":"2018","journal-title":"Med. Mal. Infect."},{"key":"ref_29","first-page":"3107","article-title":"Establishment and Application of Quantitative Real-time PCR Method to Detect Streptococcus suis Serotype 2","volume":"43","author":"Li","year":"2016","journal-title":"China Anim. Husb. Vet. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1038\/s41587-021-01049-5","article-title":"Performance assessment of DNA sequencing platforms in the ABRF Next-Generation Sequencing Study","volume":"39","author":"Jonathan","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e126","DOI":"10.1093\/nar\/gks406","article-title":"PhiSpy: A novel algorithm for finding prophages in bacterial genomes that combines similarity-and composition-based strategies","volume":"40","author":"Akhter","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_32","unstructured":"Clinical and Laboratory Standards Institute (2022). Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100, CLSI. [32nd ed.]."},{"key":"ref_33","unstructured":"National Research Council (2011). Guide for the Care and Use of Laboratory Animals, National Academies Press. [8th ed.]."},{"key":"ref_34","unstructured":"Leary, S., Underwood, W., Anthony, R., Cartner, S., Corey, D., Grandin, T., and Yanong, R.P. (2020). AVMA Guidelines for the Euthanasia of Animals: 2020 Edition, American Veterinary Medical Association."},{"key":"ref_35","unstructured":"(2018). Food Microbiology, Method 11.1: Microbiology of the Food Chain\u2014Preparation of Test Samples, Initial Suspension and Decimal Dilutions for Microbiological Examination\u2014General Rules for the Preparation of the Initial Suspension and Decimal Dilutions (ISO 6887-1:2017, MOD) (Standard No. AS 5013.11.1:2018)."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Shang, Y., Li, X., Ren, S., Du, C., Schwarz, S., Li, C., and Du, X. (2025). A novel lysin Ply691 exhibits potent bactericidal activity against Streptococcus suis. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1653748"},{"key":"ref_37","first-page":"pdb-prot4986","article-title":"Hematoxylin and eosin staining of tissue and cell sections","volume":"2008","author":"Fischer","year":"2008","journal-title":"CSH Protoc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.mimet.2004.06.007","article-title":"Eric-Pcr Fingerprinting Based Community Dna Hybridization to Pinpoint Genome Specific Fragments as Molecular Markers to Identify and Track Populations Common to Healthy Human Guts","volume":"59","author":"Wei","year":"2004","journal-title":"J. Microbiol. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kho, C., Lau, M., Chung, H., Chew, I., and Gan, H. (2023). Whole-genome sequencing of Pseudomonas koreensis isolated from diseased Tor tambroides. Curr. Microbiol., 80.","DOI":"10.1007\/s00284-023-03354-5"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1023\/A:1005870317757","article-title":"Streptococcus suis: Past and present","volume":"21","author":"Staats","year":"1997","journal-title":"Vet. Res. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kerdsin, A., Segura, M., Fittipaldi, N., and Gottschalk, M. (2022). Sociocultural Factors Influencing Human Streptococcus suis Disease in Southeast Asia. Foods, 11.","DOI":"10.3390\/foods11091190"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Dech\u00eane-Tempier, M., Marois-Cr\u00e9han, C., Libante, V., Jouy, E., Leblond-Bourget, N., and Payot, S. (2021). Update on the Mechanisms of Antibiotic Resistance and the Mobile Resistome in the Emerging Zoonotic Pathogen Streptococcus suis. Microorganisms, 9.","DOI":"10.3390\/microorganisms9081765"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e24655","DOI":"10.1002\/jcla.24655","article-title":"Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace","volume":"36","author":"Nwobodo","year":"2022","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Yongkiettrakul, S., Maneerat, K., Arechanajan, B., Malila, Y., Srimanote, P., Gottschalk, M., and Visessanguan, W. (2019). Antimicrobial susceptibility of Streptococcus suis isolated from diseased pigs, asymptomatic pigs, and human patients in Thailand. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-018-1732-5"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2320929","DOI":"10.1080\/22221751.2024.2320929","article-title":"Characterization of the diversity of type IV secretion system-encoding plasmids in Acinetobacter","volume":"13","author":"Nasser","year":"2024","journal-title":"Emerg. Microbes Infect."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Brovedan, M.A., Cameranesi, M.M., Limansky, A.S., Mor\u00e1n-Barrio, J., Marchiaro, P., and Repizo, G.D. (2020). What do we know about plasmids carried by members of the Acinetobacter genus?. World J. Microbiol. Biotechnol., 36.","DOI":"10.1007\/s11274-020-02890-7"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1038\/s41396-020-0726-z","article-title":"Modular prophage interactions driven by capsule serotype select for capsule loss under phage predation","volume":"14","author":"Buffet","year":"2020","journal-title":"ISME J."},{"key":"ref_49","first-page":"73","article-title":"Identification and genomics analysis of a clinical strain of Streptococcus suis serotype 1","volume":"53","author":"Liu","year":"2021","journal-title":"Anim. Husb. Vet. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nature10886","article-title":"RNA-guided genetic silencing systems in bacteria and archaea","volume":"482","author":"Wiedenheft","year":"2012","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/j.synbio.2024.05.009","article-title":"A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius","volume":"9","author":"Yang","year":"2024","journal-title":"Synth. Syst. Biotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"994","DOI":"10.46234\/ccdcw2021.242","article-title":"Preplanned Studies: Genomic Insight into the Antimicrobial Resistance of Streptococcus suis-Six Countries, 2011\u20132019","volume":"3","author":"Ma","year":"2021","journal-title":"China CDC Wkly."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1128\/AAC.01372-06","article-title":"Pharmacodynamic assessment based on mutant prevention concentrations of fluoroquinolones to prevent the emergence of resistant mutants of Streptococcus pneumoniae","volume":"51","author":"Homma","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1159\/000087253","article-title":"Evidence for active drug efflux in fluoroquinolone resistance in Clostridium hathewayi","volume":"51","author":"Rafii","year":"2005","journal-title":"Chemotherapy"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"101098","DOI":"10.1016\/j.onehlt.2025.101098","article-title":"Streptococcus suis: Epidemiology and resistance evolution of an emerging zoonotic bacteria","volume":"21","author":"Liu","year":"2025","journal-title":"One Health"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"105498","DOI":"10.1016\/j.dci.2025.105498","article-title":"Exploring trained immunity to complement vaccination against Streptococcus suis in swine","volume":"172","author":"Balseiro","year":"2025","journal-title":"Dev. Comp. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0378-1135(97)00131-4","article-title":"Virulence of Streptococcus suis type 2 for mice and pigs appeared host-specific","volume":"58","author":"Vecht","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1574-695X.1999.tb01369.x","article-title":"Epithelial invasion and cell lysis by virulent strains of Streptococcus suis is enhanced by the presence of suilysin","volume":"26","author":"Norton","year":"1999","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1128\/iai.59.9.3156-3162.1991","article-title":"Identification of two proteins associated with virulence of Streptococcus suis type 2","volume":"59","author":"Vecht","year":"1991","journal-title":"Infect. Immun."},{"key":"ref_60","first-page":"e33491","article-title":"Case Report: One Human Streptococcus Suis Infection in Shandong Province, China","volume":"102","author":"Zhang","year":"2021","journal-title":"Medicine"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s44149-025-00194-7","article-title":"Advances in antivirulence natural products targeting the suilysin of Streptococcus suis","volume":"5","author":"Wang","year":"2025","journal-title":"Anim. Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/11\/2552\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T05:14:27Z","timestamp":1762838067000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/11\/2552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,7]]},"references-count":61,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["microorganisms13112552"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13112552","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,7]]}}}