{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:22:57Z","timestamp":1791393777027,"version":"4.3.3"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU funding within the Next Generation EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["P E00000007, INF-ACT"],"award-info":[{"award-number":["P E00000007, INF-ACT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Serratia species are opportunistic human pathogens found in diverse environmental habitats. Here, we report the first isolation of Serratia nevei from food samples in Nigeria. During a two-month epidemiological surveillance at a local food market in Dutsin-Ma, Katsina State, Nigeria, a total of 180 food samples were collected, and isolation and species identification were performed using chromogenic agar and MicroScan autoSCAN-4, respectively. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) were determined using the MicroScan autoSCAN-4 system. Strain F129B, recovered from a fresh, unprocessed beef sample, was initially identified as Klebsiella pneumoniae by chromogenic agar and MicroScan autoSCAN-4, and subsequently as Serratia marcescens by MALDI-TOF MS. Only Whole Genome Sequencing (WGS) and bioinformatics analyses confirmed its identity as S. nevei. The strain was then selected for further characterization by Whole Genome Sequencing (WGS) and bioinformatics analyses to confirm its identity. The strain was phenotypically resistant to amoxicillin\/clavulanic acid and colistin, with elevated MICs for aztreonam (4 mg\/L) and cefuroxime (16 mg\/L). In silico analyses of its genome confirmed the isolate as S. nevei, harboring genes conferring resistance to \u03b2-lactams (blaSTR-2), aminoglycosides (aac (6\u2032)-Ic), fosfomycin (fosA), streptomycin (satA), and tetracycline (tet (41)). Its virulence repertoire comprises genes associated with adhesion (yidE, yidR, yidQ), colicin tolerance (creA and creD), and heavy metal resistance (czcD, chrBACF operon). These findings underscore the need for genomic characterization for accurate species identification within the Serratia genus. Our findings revealed the emergence of S. nevei in the food supply chain and highlighted its potential for zoonotic transmission. Robust surveillance of the local food supply chain is urgently needed in north-western Nigeria.<\/jats:p>","DOI":"10.3390\/microorganisms13122732","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:03:13Z","timestamp":1764687793000},"page":"2732","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serratia nevei in Nigeria: First Report and Global Distribution"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8573-952X","authenticated-orcid":false,"given":"Ayodele Timilehin","family":"Adesoji","sequence":"first","affiliation":[{"name":"Department of Microbiology, Federal University, Dutsin-Ma 821101, Katsina State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0968-3143","authenticated-orcid":false,"given":"Emmanuel Dayo","family":"Alabi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Federal University, Dutsin-Ma 821101, Katsina State, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2786-846X","authenticated-orcid":false,"given":"Vittoria","family":"Mattioni Marchetti","sequence":"additional","affiliation":[{"name":"Microbiology and Clinical Microbiology Unit, Scienze Clinico, Chiurgiche, Diagnostiche e Pediatriche Department, University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Migliavacca","sequence":"additional","affiliation":[{"name":"Microbiology and Clinical Microbiology Unit, Scienze Clinico, Chiurgiche, Diagnostiche e Pediatriche Department, University of Pavia, 27100 Pavia, Italy"},{"name":"Istituto di Ricovero e Cura a Carattere Scientifico Policlinico S. Matteo, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhu, W., Chen, X., Shen, H., Wei, M., Yang, C., and Gu, L. (2025). Genomic diversity, antimicrobial resistance and dissemination of Serratia marcescens complex in patients admitted to ICUs. Front. Cell. Infect. Microbiol., 15.","DOI":"10.3389\/fcimb.2025.1672468"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e00997-18","DOI":"10.1128\/mra.00997-18","article-title":"Genome sequence of the Oocydin A-producing rhizobacterium Serratia plymuthica 4Rx5","volume":"7","author":"Matilla","year":"2018","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chen, S., Blom, J., and Walker, E.D. (2017). Genomic, physiologic, and symbiotic characterization of Serratia marcescens strains isolated from the mosquito Anopheles stephensi. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.01483"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1038\/s41467-022-32929-2","article-title":"The genus Serratia revisited by genomics","volume":"13","author":"Williams","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e14399","DOI":"10.7717\/peerj.14399","article-title":"Serratia marcescens antibiotic resistance mechanisms of an opportunistic pathogen: A literature review","volume":"11","author":"Andrade","year":"2023","journal-title":"PeerJ"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cho, G.S., Stein, M., Brinks, E., Rathje, J., Lee, W., Suh, S.H., and Franz, C.M.A.P. (2020). Serratia nevei sp. nov. and Serratia bockelmannii sp. nov., isolated from fresh produce in Germany and reclassification of Serratia marcescens subsp. sakuensis Ajith Kumar et al. 2003 as a later heterotypic synonym of Serratia marcescens subsp. marcescens. Syst. Appl. Microbiol., 43, Erratum in Syst. Appl. Microbiol. 2020, 43, 126082.","DOI":"10.1016\/j.syapm.2020.126082"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Leelapsawas, C., Sroithongkham, P., Payungporn, S., Nimsamer, P., Yindee, J., Collaud, A., Perreten, V., and Chanchaithong, P. (2024). First report of blaOXA-181-carrying IncX3 plasmids in multidrug-resistant Enterobacter hormaechei and Serratia nevei recovered from canine and feline opportunistic infections. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.03589-23"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Aracil-Gisbert, S., Fern\u00e1ndez-De-Bobadilla, M.D., Guerra-Pinto, N., Serrano-Calleja, S., P\u00e9rez-Cobas, A.E., Soriano, C., de Pablo, R., Lanza, V.F., P\u00e9rez-Viso, B., and Reuters, S. (2024). The ICU environment contributes to the endemicity of the \u201cSerratia marcescens complex\u201d in the hospital setting. mBio, 15.","DOI":"10.1128\/mbio.03054-23"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e02762-23","DOI":"10.1128\/spectrum.02762-23","article-title":"A long-term survey of Serratia spp. bloodstream infections revealed an increase of antimicrobial resistance involving adult population","volume":"12","author":"Coque","year":"2024","journal-title":"Microbiol. Spectr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e01361-24","DOI":"10.1128\/spectrum.01361-24","article-title":"Complexities in species identification for Serratia marcescens complex for the modern microbiology laboratory","volume":"13","author":"Harch","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"619","DOI":"10.4314\/ahs.v22i4.67","article-title":"The occurrence of antibiotic-resistant enteric bacteria in Selected Nigerian traditional dairy products","volume":"22","author":"Uzeh","year":"2022","journal-title":"Afr. Health Sci."},{"key":"ref_12","first-page":"100458","article-title":"Antibiotic resistance and novel Sequence Types of Klebsiella spp. in human, animal, and food sources: A One Health perspective from Northern Nigeria","volume":"9","author":"Adesoji","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Aziz, R.K., Bartels, D., Best, A.A., DeJongh, M., Disz, T., Edwards, R.A., Formsma, K., Gerdes, S., Glass, E.M., and Kubal, M. (2008). The RAST Server: Rapid annotations using subsystems technology. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-75"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: A better, faster version of the PHAST phage search tool","volume":"44","author":"Arndt","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"W347","DOI":"10.1093\/nar\/gkr485","article-title":"PHAST: A fast phage search tool","volume":"39","author":"Zhou","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Shang, J., Peng, C., Liao, H., Tang, X., and Sun, Y. (2023). PhaBOX: A web server for identifying and characterizing phage contigs in metagenomic data. Bioinform. Adv., 3.","DOI":"10.1093\/bioadv\/vbad101"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Treangen, T.J., Ondov, B.D., Koren, S., and Phillippy, A.M. (2014). The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0524-x"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5539","DOI":"10.1093\/nar\/gkz361","article-title":"Fast hierarchical Bayesian analysis of population structure","volume":"47","author":"Lees","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e328","DOI":"10.1016\/S2666-5247(20)30149-X","article-title":"Definition of a genetic relatedness cutoff to exclude recent transmission of meticillin-resistant Staphylococcus aureus: A genomic epidemiology analysis","volume":"1","author":"Coll","year":"2020","journal-title":"Lancet Microbe"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Guo, C., and Wu, J.Y. (2024). Pathogen Discovery in the Post-COVID Era. Pathogens, 13.","DOI":"10.3390\/pathogens13010051"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1128\/AEM.02511-06","article-title":"Novel tetracycline resistance determinant isolated from an environmental strain of Serratia marcescens","volume":"73","author":"Thompson","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Gummalla, V.S., Zhang, Y., Liao, Y.T., and Wu, V.C.H. (2023). The Role of Temperate Phages in Bacterial Pathogenicity. Microorganisms, 11.","DOI":"10.3390\/microorganisms11030541"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.diagmicrobio.2004.02.004","article-title":"Survey of CTX-M-3 extended-spectrum \u03b2-lactamase (ESBL) among cefotaxime-resistant Serratia marcescens at a medical center in middle Taiwan","volume":"49","author":"Wu","year":"2004","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.diagmicrobio.2008.03.010","article-title":"Institutional spread of clonally related Serratia marcescens isolates with a novel AmpC cephalosporinase (S4): A 4-year experience in Taiwan","volume":"61","author":"Yu","year":"2008","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.ygeno.2018.04.001","article-title":"Genome analysis of urease positive Serratia marcescens, co-producing SRT-2 and AAC (6\u2032)-Ic with multidrug efflux pumps for antimicrobial resistance","volume":"111","author":"Srinivasan","year":"2019","journal-title":"Genomics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"125515","DOI":"10.1016\/j.envpol.2024.125515","article-title":"Monitoring sewage and effluent water is an effective approach for the detection of the mobile colistin resistance genes (mcr) and associated bacterial hosts in the human population and environment in the USA","volume":"366","author":"Hassan","year":"2025","journal-title":"Environ. Pollut."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Abreo, E., Valle, D., Gonz\u00e1lez, A., and Altier, N. (2021). Control of damping-off in tomato seedlings exerted by Serratia spp. strains and identification of inhibitory bacterial volatiles in vitro. Syst. Appl. Microbiol., 44.","DOI":"10.1016\/j.syapm.2020.126177"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Yaikhan, T., Suwannasin, S., Singkhamanan, K., Chusri, S., Pomwised, R., Wonglapsuwan, M., and Surachat, K. (2024). Genomic Characterization of Multidrug-Resistant Enterobacteriaceae Clinical Isolates from Southern Thailand Hospitals: Unraveling Antimicrobial Resistance and Virulence Mechanisms. Antibiotics, 13.","DOI":"10.3390\/antibiotics13060531"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ota, Y., Chen, F., Prah, I., Mahazu, S., Watanabe, K., Kinoshita, T., Gu, Y., Nukui, Y., and Saito, R. (2024). Metatranscriptomic Analysis Reveals Actively Expressed Antimicrobial-Resistant Genes and Their Hosts in Hospital Wastewater. Antibiotics, 13.","DOI":"10.3390\/antibiotics13121122"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kang, M., Harjadi, D., Hoover, E., Dan, H., Adam, B., and Huang, H. (2025). Complete genome sequences of six Serratia strains with multiple resistance genes isolated from food products in Canada. Microbiol. Resour. Announc., 14.","DOI":"10.1128\/mra.00301-25"},{"key":"ref_32","first-page":"100436","article-title":"Global genomic epidemiology and plasmid-mediated dissemination of blaKPC and blaNDM in the Serratia marcescens complex","volume":"9","author":"Liu","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_33","first-page":"100456","article-title":"Genomic evolution, antimicrobial resistance, and dissemination of global Serratia spp. unveil increasing species diversity and carbapenemae-resistance: A retrospective and genomic epidemiology study","volume":"9","author":"Zhu","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Peirano, G., Matsumura, Y., and Pitout, J.D.D. (Eur. J. Clin. Microbiol. Infect. Dis., 2025). Molecular epidemiology of global carbapenemase-producing Serratia spp. (2015\u20132017), Eur. J. Clin. Microbiol. Infect. Dis., Epub ahead of print.","DOI":"10.1007\/s10096-025-05254-x"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Furlan, J.P.R., Bueno, G.C., Sousa-Carmo, R.R., Silva, R.L.O., Barbosa, M.R.F., Sato, M.I.Z., Lincopan, N., and Schenkman, S. (2025). Emergence of Serratia nevei co-producing NDM-1 and CTX-M-15 in urban wastewater. Microbiol Spectr.","DOI":"10.1128\/spectrum.01378-25"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/12\/2732\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:13:12Z","timestamp":1764997992000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/12\/2732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,29]]},"references-count":35,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["microorganisms13122732"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13122732","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,29]]}}}