{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:50:32Z","timestamp":1789429832832,"version":"build-2803163510"},"reference-count":82,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key R&D projects in Henan Province","award":["241111320200"],"award-info":[{"award-number":["241111320200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["42407404"],"award-info":[{"award-number":["42407404"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The improper use of antibiotics accelerates the emergence of resistance via environmental selection pressures, jeopardizing public health and ecosystems by promoting the worldwide dissemination of antibiotic resistance genes (ARGs). Reservoirs, as crucial water supplies, have been recognized as primary reservoirs of ARGs, particularly those that originate from the Yellow River, necessitating further investigation. This study analyzed 9 ARGs, 3 mobile genetic elements (MGEs), 16 antibiotics, and 10 heavy metals in water\/sediments from three reservoirs originating from the Yellow River in Henan Province, China. The findings indicated that antibiotic concentrations in water exceeded those in sediment, with quinolones detected at 100% frequency (5.47\u2013116.03 ng\/L) and enrofloxacin predominating (3.36\u2013107.71 ng\/L). Redundancy analysis revealed that MGEs exert greater control over ARG dissemination than antibiotics, with intI1 showing strong positive correlations with sul1 (p &lt; 0.05). Conversely, heavy metals (Zn, As, Cd) suppress ARG proliferation through negative selection pressures. A network study indicated Mycobacterium, Pseudarthrobacter, and Massilia as critical hosts for ermB, tetA, and qnrA, respectively. Of the three reservoirs, Jian\u2019gang Reservoir, driven by synergistic effects of unique microbial ecology, water self-purification capacity, and flow dynamics, exhibited the best removal effectiveness of ARGs from input to outflow, with 71.75% in the water and 97.91% in the sediment. These findings provide critical insights into the prevalence, migration, and self-purification processes of ARGs in reservoirs originating from the Yellow River, integrating environmental factors and microbial data to clarify the complex dynamics affecting ARG behavior and inform targeted pollution control strategies.<\/jats:p>","DOI":"10.3390\/microorganisms13122828","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T08:30:17Z","timestamp":1765528217000},"page":"2828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Occurrence and Distribution of Antibiotics and Antibiotic Resistance Genes in the Water and Sediments of Reservoir-Based Drinking Water Sources in Henan, China"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2594-7730","authenticated-orcid":false,"given":"Wei","family":"Yuan","sequence":"first","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Shang","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingwang","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqiang","family":"Su","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luqman","family":"Riaz","sequence":"additional","affiliation":[{"name":"Department of Environmental Sciences, Kohsar University Murree, Punjab 47150, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Ecology and Environment, North China University of Water Resources and Electric Power, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"177580","DOI":"10.1016\/j.scitotenv.2024.177580","article-title":"Antibiotic-resistant Escherichia coli (E. coli) at one health interfaces in Africa: A scoping review","volume":"958","author":"Nnah","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1136\/vr.j260","article-title":"One Health perspective of antimicrobial resistance","volume":"180","year":"2017","journal-title":"Vet. Rec."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"66431","DOI":"10.1007\/s11356-023-27018-w","article-title":"Impact of antibiotics on microbial community in aquatic environment and biodegradation mechanism: A review and bibliometric analysis","volume":"30","author":"Mu","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"118236","DOI":"10.1016\/j.watres.2022.118236","article-title":"Photodegradation of organic micropollutants in aquatic environment: Importance, factors and processes","volume":"231","author":"Guo","year":"2023","journal-title":"Water Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wu, Z., Shao, X., and Wang, Q. (2025). Antibiotics and Antibiotic Resistance Genes in the Environment: Dissemination, Ecological Risks, and Remediation Approaches. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081763"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"165301","DOI":"10.1016\/j.scitotenv.2023.165301","article-title":"Biodegradation, photolysis, and sorption of antibiotics in aquatic environments: A scoping review","volume":"897","author":"Bueno","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"114513","DOI":"10.1016\/j.envres.2022.114513","article-title":"Sensitive response mechanism of ARGs and MGEs to initial designed temperature during swine manure and food waste co-composting","volume":"216","author":"Ahmed","year":"2023","journal-title":"Environ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"153186","DOI":"10.1016\/j.scitotenv.2022.153186","article-title":"Identification of critical control points for antibiotic resistance discharge in sewers","volume":"820","author":"Alexander","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.ecoenv.2018.03.083","article-title":"Antibiotics in surface water and sediments from Hanjiang River, Central China: Occurrence, behavior and risk assessment","volume":"157","author":"Hu","year":"2018","journal-title":"Ecotox Environ. Safe"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/s12302-022-00635-x","article-title":"Characterization of antibiotic resistance genes in drinking water sources of the Douhe Reservoir, Tangshan, northern China: The correlation with bacterial communities and environmental factors","volume":"34","author":"Zhang","year":"2022","journal-title":"Environ. Sci. Eur."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"065011","DOI":"10.1088\/2515-7620\/acddba","article-title":"Occurrence and distribution of antibiotics and antibiotic resistance genes in the different croplands along the Yellow River shoreline","volume":"5","author":"Zhang","year":"2023","journal-title":"Environ. Res. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1080\/1040841X.2025.2489463","article-title":"Blocking horizontal transfer of antibiotic resistance genes: An effective strategy in combating antibiotic resistance","volume":"51","author":"Swain","year":"2025","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liu, F., Luo, Y., Xu, T., Lin, H., Qiu, Y., and Li, B. (2024). Current examining methods and mathematical models of horizontal transfer of antibiotic resistance genes in the environment. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1371388"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yamin, D., Uskokovi\u0107, V., Wakil, A., Goni, M., Shamsuddin, S., Mustafa, F., Alfouzan, W., Alissa, M., Alshengeti, A., and Almaghrabi, R. (2023). Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria. Diagnostics, 13.","DOI":"10.3390\/diagnostics13203246"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1099\/jmm.0.000319","article-title":"Comparison of multiplex real-time PCR and PCR-reverse blot hybridization assay for the direct and rapid detection of bacteria and antibiotic resistance determinants in positive culture bottles","volume":"65","author":"Wang","year":"2016","journal-title":"J. Med. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105449","DOI":"10.1016\/j.envint.2019.105449","article-title":"Metagenomic insights into the profile of antibiotic resistomes in a large drinking water reservoir","volume":"136","author":"Dang","year":"2020","journal-title":"Environ. Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103907","DOI":"10.1016\/j.jwpe.2023.103907","article-title":"Drinking water sources as hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs): Occurrence, spread, and mitigation strategies","volume":"53","author":"Li","year":"2023","journal-title":"J. Water Process Eng."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7115","DOI":"10.1007\/s10653-023-01683-9","article-title":"Distribution and relationship of antibiotics, heavy metals and resistance genes in the upstream of Hanjiang River Basin in Shiyan, China","volume":"45","author":"Li","year":"2023","journal-title":"Environ. Geochem. Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"123699","DOI":"10.1016\/j.watres.2025.123699","article-title":"Unveiling the prevalence of metal resistance genes and their associations with antibiotic resistance genes in heavy metal-contaminated rivers","volume":"281","author":"Gao","year":"2025","journal-title":"Water Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"124472","DOI":"10.1016\/j.jhazmat.2020.124472","article-title":"Effects on microbiomes and resistomes and the source-specific ecological risks of heavy metals in the sediments of an urban river","volume":"409","author":"Li","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119239","DOI":"10.1016\/j.watres.2022.119239","article-title":"Removal behavior and key drivers of antibiotic resistance genes in two full-scale leachate treatment plants","volume":"226","author":"Yang","year":"2022","journal-title":"Water Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"129632","DOI":"10.1016\/j.jclepro.2021.129632","article-title":"Temporal variations of antibiotic resistance genes in influents and effluents of a WWTP in cold regions","volume":"328","author":"Sun","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_23","unstructured":"(2002). Environmental Quality Standards for Surface Water (Standard No. GB 3838-2002)."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Magoc","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5069","DOI":"10.1128\/AEM.03006-05","article-title":"Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB","volume":"72","author":"DeSantis","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.envint.2016.03.023","article-title":"Discharge of swine wastes risks water quality and food safety: Antibiotics and antibiotic resistance genes from swine sources to the receiving environments","volume":"92\u201393","author":"He","year":"2016","journal-title":"Environ. Int."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.envpol.2018.12.038","article-title":"Antibiotics in water and sediments of Danjiangkou Reservoir, China: Spatiotemporal distribution and indicator screening","volume":"246","author":"Li","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"119992","DOI":"10.1016\/j.envres.2024.119992","article-title":"Antibiotic residues in the cirata reservoir, Indonesia and their effect on ecology and the selection for antibiotic-resistant bacteria","volume":"262","author":"Ariyani","year":"2024","journal-title":"Environ. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, L., Liu, L., and Zhang, Y. (2023). Effects of Different Submerged Macrophytes on the Water and Sediment in Aquaculture Ponds with Enrofloxacin Residues. Water, 15.","DOI":"10.3390\/w15132493"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.jhazmat.2011.04.017","article-title":"Adsorption of tetracycline on soil and sediment: Effects of pH and the presence of Cu(II)","volume":"190","author":"Zhang","year":"2011","journal-title":"J. Hazard. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"139128","DOI":"10.1016\/j.scitotenv.2020.139128","article-title":"Ecotoxicological risk assessment and source apportionment of antibiotics in the waters and sediments of a peri-urban river","volume":"731","author":"Zhang","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"112207","DOI":"10.1016\/j.ecoenv.2021.112207","article-title":"Adsorption behavior of organic pollutants on microplastics","volume":"217","author":"Fu","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.chemosphere.2008.12.060","article-title":"Degradation of oxolinic acid and flumequine in aquaculture pond waters and sediments","volume":"75","author":"Lai","year":"2009","journal-title":"Chemosphere"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"26045","DOI":"10.1007\/s11356-021-18251-2","article-title":"Migration, Transformation and Removal of Macrolide Antibiotics in The Environment: A Review","volume":"29","author":"Yuan","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/j.scitotenv.2019.03.393","article-title":"Spatial distribution, source apportionment and risk assessment of antibiotics in the surface water and sediments of the Yangtze Estuary","volume":"671","author":"Guo","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"105766","DOI":"10.1016\/j.catena.2021.105766","article-title":"Quantification of soil element changes in long-term agriculture: A case study in Northeast China","volume":"208","author":"Zhang","year":"2022","journal-title":"Catena"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109583","DOI":"10.1016\/j.jenvman.2019.109583","article-title":"Profiles of ARGs and their relationships with antibiotics, metals and environmental parameters in vertical sediment layers of three lakes in China","volume":"255","author":"Ohore","year":"2020","journal-title":"J. Environ. Manag."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.jtemb.2018.02.009","article-title":"Zinc in soils, water and food crops","volume":"49","author":"Noulas","year":"2018","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.jssas.2021.06.001","article-title":"Effects of long-term agricultural activities on the availability of heavy metals in Syrian soil: A case study in southern Syria","volume":"20","author":"Mohammed","year":"2021","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1038\/ismej.2011.15","article-title":"Impacts of anthropogenic activity on the ecology of class 1 integrons and integron-associated genes in the environment","volume":"5","author":"Gaze","year":"2011","journal-title":"ISME J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1021\/acs.est.6b05887","article-title":"The Prevalence of Integrons as the Carrier of Antibiotic Resistance Genes in Natural and Man-Made Environments","volume":"51","author":"Ma","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1016\/j.envpol.2019.06.095","article-title":"Persistence and migration of tetracycline, sulfonamide, fluoroquinolone, and macrolide antibiotics in streams using a simulated hydrodynamic system","volume":"252","author":"Liu","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"115381","DOI":"10.1016\/j.jece.2025.115381","article-title":"Metagenomic assembly insight into the antibiotic resistance genes and antibiotic resistant bacteria in packaged drinking water system","volume":"13","author":"Xia","year":"2025","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.chemosphere.2017.01.041","article-title":"Distribution of antibiotic resistance in the effluents of ten municipal wastewater treatment plants in China and the effect of treatment processes","volume":"172","author":"Ben","year":"2017","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.marpolbul.2016.03.064","article-title":"Occurrence and distribution of antibiotic resistance genes in the coastal area of the Bohai Bay, China","volume":"107","author":"Niu","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jes.2018.06.011","article-title":"Occurrence of antibiotic resistance genes and bacterial pathogens in water and sediment in urban recreational water","volume":"77","author":"Dong","year":"2019","journal-title":"J. Environ. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s12302-019-0265-2","article-title":"Occurrence and distribution of antibiotic resistance genes in the water and sediments of Qingcaosha Reservoir, Shanghai, China","volume":"31","author":"Huang","year":"2019","journal-title":"Environ. Sci. Eur."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"119144","DOI":"10.1016\/j.jenvman.2023.119144","article-title":"Unraveling the antibiotic resistome in backwater zones of large cascade reservoirs: Co-occurrence patterns, horizontal transfer directions and health risks","volume":"347","author":"Wang","year":"2023","journal-title":"J. Environ. Manag."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1021\/es404280v","article-title":"Persistence of Extracellular DNA in River Sediment Facilitates Antibiotic Resistance Gene Propagation","volume":"48","author":"Mao","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"123947","DOI":"10.1016\/j.jenvman.2024.123947","article-title":"Differential insights into the distribution characteristics of bacterial communities and their response to typical pollutants in the sediment and soil of large drinking water reservoir","volume":"373","author":"Yang","year":"2025","journal-title":"J. Environ. Manag."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"127697","DOI":"10.1016\/j.jclepro.2021.127697","article-title":"Characteristics of spatial distribution for microbial ecology inside and outside source water reservoir","volume":"311","author":"Guo","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1038\/s41467-021-22757-1","article-title":"Forecasting the dissemination of antibiotic resistance genes across bacterial genomes","volume":"12","author":"Ellabaan","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.scitotenv.2018.08.212","article-title":"Compost-bulking agents reduce the reservoir of antibiotics and antibiotic resistance genes in manures by modifying bacterial microbiota","volume":"649","author":"Zhang","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Lajqi Berisha, N., Poceva Panovska, A., and Hajrulai-Musliu, Z. (2024). Antibiotic Resistance and Aquatic Systems: Importance in Public Health. Water, 16.","DOI":"10.3390\/w16172362"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1007\/s00253-020-11047-3","article-title":"Changes in planktonic and sediment bacterial communities under the highly regulated dam in the mid-part of the Three Gorges Reservoir","volume":"105","author":"Qin","year":"2021","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1007\/s00253-016-8019-1","article-title":"Spatiotemporal variation of bacterial and archaeal communities in sediments of a drinking reservoir, Beijing, China","volume":"101","author":"Chen","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s13213-013-0712-z","article-title":"Bacterial communities in sediments of a drinking water reservoir","volume":"64","author":"Cheng","year":"2014","journal-title":"Ann. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"117801","DOI":"10.1016\/j.envres.2023.117801","article-title":"Mobile genetic elements affect the dissemination of antibiotic resistance genes (ARGs) of clinical importance in the environment","volume":"243","author":"Li","year":"2024","journal-title":"Environ. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.procbio.2012.12.016","article-title":"Changes of biomass and bacterial communities in biological activated carbon filters for drinking water treatment","volume":"48","author":"Liao","year":"2013","journal-title":"Process Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1038\/s41579-023-00933-y","article-title":"Antibiotic perturbations to the gut microbiome","volume":"21","author":"Fishbein","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.scitotenv.2016.12.141","article-title":"Biodegradation of the veterinary antibiotics enrofloxacin and ceftiofur and associated microbial community dynamics","volume":"581\u2013582","author":"Alexandrino","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/s10661-022-10314-2","article-title":"Microbial degradation of antibiotic: Future possibility of mitigating antibiotic pollution","volume":"194","author":"Kayal","year":"2022","journal-title":"Environ. Monit. Assess."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.chemosphere.2016.02.064","article-title":"Biodegradation of sulfonamide antibiotics in sludge","volume":"150","author":"Yang","year":"2016","journal-title":"Chemosphere"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"131208","DOI":"10.1016\/j.jhazmat.2023.131208","article-title":"Mariculture affects antibiotic resistome and microbiome in the coastal environment","volume":"452","author":"He","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"50952","DOI":"10.1007\/s11356-024-34590-2","article-title":"Distribution and community structure of antibiotic resistance genes in the Three Gorges Reservoir Area","volume":"31","author":"Han","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.envint.2018.10.032","article-title":"Prevalence and transmission of antibiotic resistance and microbiota between humans and water environments","volume":"121","author":"Zhou","year":"2018","journal-title":"Environ. Int."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.envint.2018.04.028","article-title":"The abundance and diversity of antibiotic resistance genes in the atmospheric environment of composting plants","volume":"116","author":"Gao","year":"2018","journal-title":"Environ. Int."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"158811","DOI":"10.1016\/j.scitotenv.2022.158811","article-title":"Climate and nutrients regulate biographical patterns and health risks of antibiotic resistance genes in mangrove environment","volume":"854","author":"Zhao","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1038\/s41467-022-29283-8","article-title":"Assessment of global health risk of antibiotic resistance genes","volume":"13","author":"Zhang","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.mimet.2011.07.016","article-title":"Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment","volume":"87","author":"Contreras","year":"2011","journal-title":"Microbiol. Methods"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1128\/AAC.45.3.723-726.2001","article-title":"Incidence of Class 1 and 2 Integrases in Clinical and Commensal Bacteria from Livestock, Companion Animals, and Exotics","volume":"45","author":"Goldstein","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"105456","DOI":"10.1016\/j.ecolind.2019.105456","article-title":"Macrolide- and quinolone-resistant bacteria and resistance genes as indicators of antibiotic resistance gene contamination in farmland soil with manure application","volume":"106","author":"Wang","year":"2019","journal-title":"Ecol. Indic."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1128\/AAC.01668-09","article-title":"qnrVC-Like Gene Located in a Novel Complex Class 1 Integron Harboring the ISCR1 Element in an Aeromonas punctata Strain from an Aquatic Environment in Shandong Province, China","volume":"54","author":"Xia","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/j.1462-2920.2006.01185.x","article-title":"Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months","volume":"9","author":"Heuer","year":"2007","journal-title":"Environ. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"101315","DOI":"10.1016\/j.genrep.2021.101315","article-title":"The existence and expression of aminoglycoside resistance genes among multidrug-resistant Escherichia coli isolates in intensive care unit centers","volume":"25","author":"Alneama","year":"2021","journal-title":"Gene Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"106470","DOI":"10.1016\/j.marenvres.2024.106470","article-title":"Occurrence and distribution of antibiotics and antibiotic resistance genes from the land to ocean in Daliao River-Liaodong Bay, China","volume":"197","author":"Xu","year":"2024","journal-title":"Mar. Environ. Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"121803","DOI":"10.1016\/j.biortech.2019.121803","article-title":"Analysis for microbial denitrification and antibiotic resistance during anaerobic digestion of cattle manure containing antibiotic","volume":"291","author":"Zhang","year":"2019","journal-title":"Bioresour. Technol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"122792","DOI":"10.1016\/j.biortech.2020.122792","article-title":"Long-term alkaline conditions inhibit the relative abundances of tetracycline resistance genes in saline 4-chlorophenol wastewater treatment","volume":"301","author":"Li","year":"2020","journal-title":"Bioresour. Technol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"134626","DOI":"10.1016\/j.scitotenv.2019.134626","article-title":"Prevalence and distribution analysis of antibiotic resistance genes in a large-scale aquaculture environment","volume":"711","author":"Shen","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"134899","DOI":"10.1016\/j.scitotenv.2019.134899","article-title":"Impact of roxithromycin on waste activated sludge anaerobic digestion: Methane production, carbon transformation and antibiotic resistance genes","volume":"703","author":"Ni","year":"2020","journal-title":"Sci. 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