{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T14:42:29Z","timestamp":1789828949436,"version":"4.0.1"},"reference-count":43,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Research Development Program of China","award":["2024YFC2607500"],"award-info":[{"award-number":["2024YFC2607500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Ticks transmit diverse pathogens, posing significant public health threats in northeastern China; clarifying human-biting tick species and their carried pathogens is crucial for risk assessment and prevention. This study was designed as a pathogen detection and molecular epidemiological investigation. During the May-August period of each of 2023 and 2024, 232 ticks that had bitten humans were collected from a local sentinel hospital. Ticks were morphologically identified, followed by DNA extraction, semi-nested\/nested PCR targeting specific genetic markers, and Sanger sequencing to detect bacterial pathogens. Four tick species were found, with Ixodes persulcatus dominant (87.9%), followed by Dermacentor silvarum (6.9%), Haemaphysalis concinna (3.5%), and Haemaphysalis japonica (1.7%). Seven bacterial pathogens were detected, including Candidatus Rickettsia tarasevichiae (35.3%) and Borreliella garinii (17.2%); 49.6% of ticks were pathogen-positive. The coinfection rate was 13.8%, most commonly Candidatus R. tarasevichiae plus B. garinii, and I. persulcatus carried all pathogens. These findings provide basic data on human-biting tick species and their bacterial pathogen spectrum in the region, serving as a reference for subsequent tick-borne disease research and targeted prevention.<\/jats:p>","DOI":"10.3390\/microorganisms13122862","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T14:36:53Z","timestamp":1765895813000},"page":"2862","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Variety of Bacterial Pathogens in Ticks Removed from Humans, Northeastern China"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6754-2644","authenticated-orcid":false,"given":"Xiao-Ling","family":"Su","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Ling","family":"Ye","sequence":"additional","affiliation":[{"name":"Mudanjiang Forestry Central Hospital, Mudanjiang 157011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Zhu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of EcoHealth, School of Public Health, Shandong University, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of EcoHealth, School of Public Health, Shandong University, Jinan 250012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6871-6975","authenticated-orcid":false,"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Fei","family":"Wu","sequence":"additional","affiliation":[{"name":"National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102629, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cai","family":"Bian","sequence":"additional","affiliation":[{"name":"Mudanjiang Forestry Central Hospital, Mudanjiang 157011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan-Nan","family":"Yao","sequence":"additional","affiliation":[{"name":"Mudanjiang Forestry Central Hospital, Mudanjiang 157011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Chun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Mudanjiang Forestry Central Hospital, Mudanjiang 157011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Fu","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"Mudanjiang Forestry Central Hospital, Mudanjiang 157011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu-Chun","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.pt.2012.07.003","article-title":"Ticks and Tick-Borne Diseases: A One Health Perspective","volume":"28","author":"Chomel","year":"2012","journal-title":"Trends Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1056\/NEJMp1807870","article-title":"Tickborne Diseases\u2014Confronting a Growing Threat","volume":"379","author":"Paules","year":"2018","journal-title":"N. 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