{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T05:17:34Z","timestamp":1767503854851,"version":"3.48.0"},"reference-count":69,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Thalassogenic diseases are human infections associated with exposure to marine environments. This review explores the occurrence of Cryptosporidium spp., Giardia duodenalis, and Blastocystis sp. in seawater and shellfish and their implications for public health. Between 2015 and 2026, multiple studies reported the presence of these parasites in shellfish and seawater. Cryptosporidium spp. was found at average concentrations of 5.5 \u00d7 101 oocysts\/g in shellfish and up to 3.7 \u00d7 101 oocysts\/L in seawater. Giardia duodenalis reached 9.1 \u00d7 101 cysts\/g in shellfish, close to the infectious dose, and 3.5 \u00d7 101 cysts\/L in seawater. Blastocystis sp. showed prevalence rates of 33.82% in shellfish and 17.3% in seawater. These findings highlight a potential infection risk for bathers and seafood consumers, emphasizing the need to determine the specific species (or subtypes) involved and assess their viability to accurately evaluate public health implications. The persistence of these parasites in the environment needs improved monitoring. Future strategies should integrate next-generation sequencing (NGS) or use of various fecal indicators to enhance environmental surveillance and reduce health risks in coastal regions.<\/jats:p>","DOI":"10.3390\/microorganisms14010098","type":"journal-article","created":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:51:21Z","timestamp":1767376281000},"page":"98","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Waterborne Protozoan Parasite and Thalassogenic Diseases in Marine Environment: Detection Techniques, Indicators and Public Health Implications"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1985-069X","authenticated-orcid":false,"given":"Pilar","family":"Suarez","sequence":"first","affiliation":[{"name":"Environmental Engineering & Biotechnology Group (GIBA-UDEC), Environmental Science Faculty & EULA-CHILE Center, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Water Research Center for Agriculture and Mining (CRHIAM), ANID Fondap Center, Victoria 1295, Concepci\u00f3n 4070411, Chile"},{"name":"Laboratorio de Parasitolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-910X","authenticated-orcid":false,"given":"Jos\u00e9 Lu\u00eds","family":"Alonso","sequence":"additional","affiliation":[{"name":"Instituto de Ingenier\u00eda del Agua y Medio Ambiente, Universidad Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7433-5004","authenticated-orcid":false,"given":"Gladys","family":"Vidal","sequence":"additional","affiliation":[{"name":"Environmental Engineering & Biotechnology Group (GIBA-UDEC), Environmental Science Faculty & EULA-CHILE Center, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Water Research Center for Agriculture and Mining (CRHIAM), ANID Fondap Center, Victoria 1295, Concepci\u00f3n 4070411, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/s41558-022-01426-1","article-title":"Over half of known human pathogenic diseases can be aggravated by climate change","volume":"12","author":"Mora","year":"2022","journal-title":"Nat. Clim. Change"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1001\/jama.2025.7123","article-title":"Climate change, marine pathogens and human health","volume":"334","author":"Semenza","year":"2025","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.2166\/wh.2023.094","article-title":"Waterborne transmission of protozoan parasite: A review of worldwide out-breaks\u2014An update 2017\u20132022","volume":"23","author":"Bourli","year":"2023","journal-title":"J. Water Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.scitotenv.2019.05.415","article-title":"Fecal pollution source tracking and thalassogenic diseases: The temporal spatial concordance between maximum concentration of human mitochondrial DNA in seawater and Hepatitis A outbreaks among a coastal population","volume":"686","author":"Soto","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_5","unstructured":"Shuval, H.I. (2025, December 05). Thalassogenic Diseases. UNEP Regional Reports and Studies 1986, N\u00b0 79. United Nations Environment Programme. Available online: https:\/\/www.unep.org\/resources\/report\/thalassogenic-diseases."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"53","DOI":"10.2166\/wh.2003.0007","article-title":"Estimating the global burden of thalassogenic diseases: Human infectious diseases caused by wastewater pollution of the marine environment","volume":"1","author":"Shuval","year":"2003","journal-title":"J. Water Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1080\/10807039.2025.2464110","article-title":"Appliyng epidemiology and quantitative microbial risk assessment to ambient water quality evaluation: Case study of fecal contaminated water in a US inland lake","volume":"31","author":"Ravenscroft","year":"2025","journal-title":"Hum. Ecol. Risk Assess."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1007\/s00436-010-1769-2","article-title":"Relationships among bather density, levels of human waterborne pathogens, and fecal coliform counts in marine recreational beach water","volume":"106","author":"Graczyk","year":"2010","journal-title":"Parasitol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.watres.2017.01.036","article-title":"Waterborne transmission of protozoan parasite: Review of worldwide outbreaks\u2014An update 2011\u20132016","volume":"114","author":"Efstratiou","year":"2017","journal-title":"Water Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100311","DOI":"10.1016\/j.crpvbd.2025.100311","article-title":"Cryptosporidium and cryptosporidiosis: An update of Asian perspective in humans, water and food, 2015\u20132025","volume":"8","author":"Ahmed","year":"2025","journal-title":"Curr. Res. Parasitol. Vector-Borne Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mib.2016.07.019","article-title":"An up-date on Giardia and giardiasis","volume":"34","author":"Einarsson","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"106455","DOI":"10.1016\/j.actatropica.2022.106455","article-title":"Evaluation of association with subtypes and alleles of Blastocystis with chronic spontaneous urticaria","volume":"231","author":"Aykur","year":"2022","journal-title":"Acta Trop."},{"key":"ref_13","unstructured":"World Health Organization (WHO) (2017). Guidelines for Drinking-Water Quality: Fourth Edition Incorporating the First Addendum, World Health Organization."},{"key":"ref_14","unstructured":"(2025, September 18). Supreme Decret 90 Laws, Chile N\u00b01475. Diario Oficial, 7 March 2000. Available online: https:\/\/www.bcn.cl\/leychile\/navegar?idNorma=182637."},{"key":"ref_15","first-page":"S45","article-title":"Characterization of Cryptosporidium isolated from Asian Green mussels sold in wet markets of Quezon city, Philippines","volume":"100","author":"Pago","year":"2017","journal-title":"Philipp. Agric. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"243","DOI":"10.4067\/s0716-10182020000300257","article-title":"Detection of Cryptosporidium parvum (Apicomplexa: Cryptosporidiidae) oocyst in Aulacomya ater specimen, extracted from to the coast Biob\u00edo region, Chile","volume":"37","author":"Suarez","year":"2020","journal-title":"Rev. Chil. Infectol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e00205","DOI":"10.1016\/j.fawpar.2023.e00205","article-title":"Occurrence of Cryptosporidium oocyst in commercial oysters in southern Thailand","volume":"32","author":"Srisuphanunt","year":"2023","journal-title":"Food Waterborne Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00214","DOI":"10.1016\/j.fawpar.2023.e00214","article-title":"Identification of Cryptosporidium parvum and Blastocystis hominis subtype ST3 in Cholga mussel and treated sewage: Preliminary evidence of fecal contamination in harvesting area","volume":"34","author":"Suarez","year":"2024","journal-title":"Food Waterborne Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ijppaw.2016.01.004","article-title":"Mussels (Perna perna) as bioindicator of environmental contamination by Cryptosporidium species with zoonotic potential","volume":"5","author":"Oliveira","year":"2016","journal-title":"Int. J. Parasitol. Parasite Wildl."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tei, F.F., Kowalyk, S., Reid, J.A., Presta, M.A., Yesuda, R., and Mayer, D.C.G. (2016). Assessment and molecular characterization of human intestinal parasites in bivalve from Orchard beach, NY, USA. Int. J. Environ. Res. Public Health, 13.","DOI":"10.3390\/ijerph13040381"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.marpolbul.2017.01.057","article-title":"First report of Tunisian coastal water contamination by protozoan parasites using mollusk bivalve as biological indicators","volume":"117","author":"Ghozzi","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1007\/s00436-018-5832-8","article-title":"First report of Toxoplasma gondii sporulated oocyst and Giardia duodenalis in commercial green-lipped mussels (Perna canaliculus) in New Zealand","volume":"117","author":"Coupe","year":"2018","journal-title":"Parasitol. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"S25","DOI":"10.4315\/0362-028X.JFP-18-157","article-title":"Toxoplasma gondii and other zoonotic protozoan in Mediterranean mussel (Mytilus galloprovincialis) and blue mussel (Mytilus edulis): A food safety concern?","volume":"82","author":"Tedde","year":"2019","journal-title":"J. Food Prot."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/zph.12693","article-title":"Cryptosporidium and Giardia in locally harvested clams in Iqaluit, Nunavut","volume":"67","author":"Manore","year":"2020","journal-title":"Zoonoses Public Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"107848","DOI":"10.1016\/j.exppara.2020.107848","article-title":"Investigations from Northern Greece on mussels cultivated in areas proximal to wastewaters discharges as a potential source for human infections with Giardia and Cryptosporidium","volume":"210","author":"Ligda","year":"2020","journal-title":"Exp. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e20210014","DOI":"10.1590\/0103-8478cr20210014","article-title":"Cryptosporidium sp. in cultivated oysters and the natural oyster stock of the state of Maranhao, Brazil","volume":"53","author":"Silva","year":"2023","journal-title":"Cienc. Rural."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Merks, H., Boone, R., Janecko, N., Viswanathan, M., and Dixon, B.R. (2023). Foodborne protozoan parasites in fresh mussels and oyster purchased at retail in Canada. Int. J. Food Microbiol., 399.","DOI":"10.1016\/j.ijfoodmicro.2023.110248"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4655","DOI":"10.1007\/s00436-015-4711-9","article-title":"The development and implementation of a method using blue mussels (Mytilus spp.) as biosentinels of Cryptosporidium spp. and Toxoplasma gondii contamination in marine aquatic environments","volume":"114","author":"Staggs","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.marpolbul.2017.03.005","article-title":"Cryptosporidium species from common edible bivalves in Manila Bay, Philippines","volume":"119","author":"Pagoso","year":"2017","journal-title":"Mar. Pollut. Bull."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"23129","DOI":"10.1007\/s11356-020-08650-2","article-title":"Assessment of health risk from recreational exposure to Giardia and Cryptosporidium in coastal bathing waters","volume":"27","author":"Pinto","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"116507","DOI":"10.1016\/j.watres.2020.116507","article-title":"Among microbial indicators of fecal pollution, microbial source tracking markers and pathogens in Costa Rica coastal waters","volume":"188","author":"Symonds","year":"2021","journal-title":"Water Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"114716","DOI":"10.1016\/j.marpolbul.2023.114716","article-title":"Occurrence of enteric and non-enteric microorganisms in coastal waters impacted by anthropogenic activities: A multi-route QMRA for swimmers","volume":"188","author":"Gholipour","year":"2023","journal-title":"Mar. Pollut. Bull."},{"key":"ref_33","first-page":"1033956","article-title":"A two-year monitoring of Cryptosporidium spp. oocysts and Giardia spp. cysts in freshwater and seawater: A complementary strategy for measuring sanitary patterns of recreational tropical coastal areas from Brazil","volume":"70","author":"Bonatti","year":"2024","journal-title":"Reg. Stud. Mar. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Suarez, P., Fern\u00e1ndez, I., Alonso, J.L., and Vidal, G. (2025). Evidence of waterborne parasite in mussels for human consumption harvested from a recreational and highly productive bay. Microorganisms, 13.","DOI":"10.3390\/microorganisms13091971"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ryckman, M., Gantois, N., Garc\u00eda, R., Desramaut, J., Li, L., Even, G., Audebert, C., Devos, D., Chab\u00e9, M., and Certad, G. (2024). Molecular identification and subtype analysis of Blastocystis sp. isolates from wild mussels (Mytilus edulis) in Northern France. Microorganisms, 12.","DOI":"10.3390\/microorganisms12040710"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"111730","DOI":"10.1016\/j.foodcont.2025.111730","article-title":"First molecular detection of Blastocystis sp. in Mediterranean mussels Mytilus galloprovincialis from the western coast of Italy: Seafood safety and environmental contamination implications","volume":"181","author":"Andolf","year":"2026","journal-title":"Food Control"},{"key":"ref_37","first-page":"56","article-title":"Molecular identification of Blastocystis sp. subtypes in water samples collected from black sea, Turkey","volume":"180","author":"Kolorean","year":"2018","journal-title":"Acta Trop."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Gomes-Goncalves, S., Machado, A., Borlado, A., and Mesquita, J.R. (2025). Anthropogenic Blastocystis from drinking well and coastal water in Guinea-Bissau (West Africa). Microorganisms, 13.","DOI":"10.3390\/microorganisms13030620"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"112322","DOI":"10.1016\/j.jece.2024.112322","article-title":"Performance of full-scale rural wastewater treatment plants in the reduction of antibiotic-resistant bacteria and antibiotic resistance genes from small-city effluents","volume":"12","author":"Leiva","year":"2024","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_40","first-page":"1870","article-title":"Disinfection behavior of a UV-treated wastewater system using constructed wetlands and the rate of reactivation of pathogenic microorganisms","volume":"80","author":"Salgado","year":"2020","journal-title":"Water Sci. Technol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.ijpara.2021.08.007","article-title":"Taxonomy and molecular epidemiology of Cryptosporidium and Giardia\u2014A 50 year perspective (1971\u20132021)","volume":"51","author":"Ryan","year":"2021","journal-title":"Int. J. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"116320","DOI":"10.1016\/j.jenvman.2022.116320","article-title":"Performance of sewage treatment technologies for the removal of Cryptosporidium sp. and Giardia sp.: Toward water circularity","volume":"324","author":"Suarez","year":"2022","journal-title":"J. Environ. Manag."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e00280","DOI":"10.1016\/j.fawpar.2025.e00280","article-title":"Molecular identification and genotyping of Blastocystis in farmed cattle, goats and pigs from Zhejiang province, China","volume":"40","author":"Zhao","year":"2025","journal-title":"Food Waterborne Parasitol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"107757","DOI":"10.1016\/j.ocecoaman.2025.107757","article-title":"Microbial contamination in the marine recreational sites and its impact on public health","volume":"267","author":"Gopinathan","year":"2025","journal-title":"Ocean Coast. Manage"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1556\/1886.2019.00019","article-title":"Cryptosporidium infection: Epidemiology, pathogenesis, and differential diagnosis","volume":"9","author":"Gerace","year":"2019","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"ref_46","first-page":"910","article-title":"Prevalence and clinical manifestation of Giardia intestinalis and other intestinal parasite in children and adults in Algeria","volume":"104","author":"Belkessa","year":"2021","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.actatropica.2018.01.011","article-title":"Methods for the detection of Cryptosporidium and Giardia: From microscopy to nucleic acid based tools in clinical and environmental regimes","volume":"184","author":"Adeyemo","year":"2018","journal-title":"Acta Trop."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.4315\/0362-028X.JFP-13-002","article-title":"Tools and methods for detecting and characterizing Giardia, Cryptosporidium and Toxoplasma parasites in marine mollusks","volume":"76","author":"Hohweyer","year":"2013","journal-title":"J. Food Prot."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ijfoodmicro.2019.03.009","article-title":"Protocol standardization for the detection of Giardia cyst and Cryptosporidium oocyst in Mediterranean mussels (Mytilus galloprovincialis)","volume":"298","author":"Ligda","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_50","first-page":"736","article-title":"Blue mussel (Mytilus edulis) a bioindicator of marine water contamination by protozoa: Laboratory and in situ approaches","volume":"132","author":"Cazeaun","year":"2020","journal-title":"J. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kim, M., Rueda, L., Packham, A., Moore, J., Wuertz, S., and Shapiro, K. (2023). Molecular detection and viability discrimination of zoonotic protozoan pathogens in oysters and seawater. Int. J. Food Microbiol., 407.","DOI":"10.1016\/j.ijfoodmicro.2023.110391"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"109132","DOI":"10.1016\/j.jece.2022.109132","article-title":"Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering membrane for culture-independent microbiota analysis of clean waters","volume":"11","author":"Pedrosa","year":"2023","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2881(08)60210-2","article-title":"Mucus from Marine Mollusks","volume":"34","author":"Davies","year":"1998","journal-title":"Adv. Mar. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Schenk, J.J., Becklund, L.E., Carey, L.E., Carey, S.J., and Fabre, P. (2023). What is the \"modified CTAB protocol\"? Characterizing modifications to the CTAB DNA extraction protocol. Appl. Plant Sci., 11.","DOI":"10.1002\/aps3.11517"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"174219","DOI":"10.1016\/j.scitotenv.2024.174219","article-title":"Evaluation of molecular-based methods for the detection and quantification of Cryptosporidium spp. in wastewater","volume":"947","author":"Hachimi","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.pt.2007.01.004","article-title":"Terminology for Blastocystis subtypes\u2014A consensus","volume":"23","author":"Stensvold","year":"2006","journal-title":"Trends Parasitol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"100292","DOI":"10.1016\/j.crpvbd.2025.100292","article-title":"A review of recent Cryptosporidium hominis and Cryptosporidium parvum gp60 subtypes","volume":"8","author":"Oladele","year":"2025","journal-title":"Curr. Res. Parasitol. Vector-Borne Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/978-981-13-8844-6_15","article-title":"Next Generation Sequencing and its applications: Empowering in public health beyond reality","volume":"Volume 17","author":"Kumar","year":"2019","journal-title":"Microbial Technology for Welfare of Society"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"DeMone, C., McClure, J.T., Greenwood, S.J., Fung, R., Hwang, M.H., Feng, Z., and Shapiro, K. (2021). A metabarcording approach for detecting protozoan pathogens in wild oyster from Prince Edward Island, Canada. Int. J. Food Microbiol., 360.","DOI":"10.1016\/j.ijfoodmicro.2021.109315"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.watres.2019.04.041","article-title":"Identification of eukarytic microorganism with 18S rRNA next-generation sequencing in wastewater treatment plants, with a more target NGS approach required for Cryptosporidium detection more targeted NGS approach required for Cryptosporidium detection","volume":"158","author":"Zahedi","year":"2019","journal-title":"Water Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.scitotenv.2018.07.024","article-title":"Profiling the diversity of Cryptosporidium species and genotype in wastewater treatment plants in Australia using next generation sequencing","volume":"644","author":"Zahedi","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"169602","DOI":"10.1016\/j.scitotenv.2023.169602","article-title":"Profiling pathogenic protozoan and their functional pathways in wastewater using 18rRNA and shotgun metagenomics","volume":"912","author":"Mthethwa","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1128\/aem.59.8.2418-2424.1993","article-title":"Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and protozoan cysts","volume":"59","author":"Payment","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_64","first-page":"285","article-title":"Spores of sulphite-reducing clostridia: A surrogate parameter for assessing the effects of water treatment processes on protozoan oocysts","volume":"35","author":"Hijnen","year":"1997","journal-title":"Water Sci. Tech."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"4448","DOI":"10.1007\/s11356-012-1400-4","article-title":"Alternative indicators for monitoring Cryptosporidium oocyst in reclaimed water","volume":"20","author":"Oliva","year":"2013","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"491","DOI":"10.2166\/wh.2018.013","article-title":"Use of bacterial spores in monitoring water quality and treatment","volume":"16","author":"Stelma","year":"2018","journal-title":"J. Water Health"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"105430","DOI":"10.1016\/j.meegid.2023.105430","article-title":"Proposed taxonomic revision of Giardia duodenalis","volume":"111","author":"Wielinga","year":"2023","journal-title":"Infect. Genet. Evol."},{"key":"ref_68","first-page":"e2592","article-title":"An update on the distribution of Blastocystis subtype in the Americas","volume":"8","author":"Jimenez","year":"2023","journal-title":"Heliyon"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1186\/s13071-019-3641-2","article-title":"A summary of Blastocystis subtypes in north and south America","volume":"12","author":"Jimenez","year":"2019","journal-title":"Parasite Vec."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/1\/98\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T05:13:28Z","timestamp":1767503608000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/1\/98"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,2]]},"references-count":69,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["microorganisms14010098"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14010098","relation":{},"ISSN":["2076-2607"],"issn-type":[{"type":"electronic","value":"2076-2607"}],"subject":[],"published":{"date-parts":[[2026,1,2]]}}}