{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:54:51Z","timestamp":1767603291038,"version":"3.48.0"},"reference-count":52,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T00:00:00Z","timestamp":1767484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Blood culture remains the gold standard for identifying bloodstream infections caused by bacteria and fungi. Isolation of the culprit microorganism onto agar plates also facilitates antimicrobial susceptibility testing. The purpose of this study was to determine the contamination rates, pathogen profile, and antimicrobial resistance in a secondary healthcare setting in a two-year timeframe. In this study, data regarding blood cultures of the years 2023 and 2024 were retrospectively analyzed to address the above questions. Blood cultures were incubated for seven days before being discarded as negative. The percentage of positive blood cultures for both years was 14.3%. Most positive cultures contained Gram-positive cocci, with a prevalence of coagulase-negative Staphylococci. In descending order, 72.72% were coagulase-negative Staphylococci, 15.15% were Staphylococcus aureus, and 12.12% were Streptococci. One strain of S. aureus was methicillin-resistant (MRSA), and one strain of Enterococcus faecium was vancomycin-resistant (VRE). Of the Gram-negative rods, 78.3% were Enterobacterales. Of these, Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis were the top pathogens. The remainder comprised eight strains of Pseudomonas aeruginosa, four strains of Acinetobacter baumannii (one pandrug-resistant), three strains of Stenotrophomonas maltophilia, one strain of Sphingomonas paucimobilis, and one strain of Campylobacter jejuni. The isolated fungi comprised Candida parapsilosis, Candida glabrata, and Candida tropicalis. Of the isolated Escherichia coli strains, 39.5% were resistant to ciprofloxacin regardless of origin (outpatient or hospitalized patients). Outpatient samples were taken in a Hemodialysis Unit that collaborates with our laboratory, obtained from patients with fever or other signs of infection. Distinguishing true bacteremia from contamination remains challenging. The contamination rate in our study was quite high at 5.3%. Since there is no dedicated phlebotomy team in our healthcare setting, in light of our results, educational courses have been conducted to demonstrate the best practices for sample collection.<\/jats:p>","DOI":"10.3390\/microorganisms14010107","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T08:40:53Z","timestamp":1767602453000},"page":"107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Two-Year Retrospective Study of Blood Cultures in a Secondary Western Greece Healthcare Setting"],"prefix":"10.3390","volume":"14","author":[{"given":"Eirini","family":"Tsolakidou","sequence":"first","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6079-4921","authenticated-orcid":false,"given":"Ioannis","family":"Angelidis","sequence":"additional","affiliation":[{"name":"Department of Radiology, General Hospital of Eastern Achaia, 25001 Kalavrita, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apostolos","family":"Asproukos","sequence":"additional","affiliation":[{"name":"Infection Preventionist Nurse, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aikaterini","family":"Chalmouki","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Zalavras","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyriakos","family":"Louca","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panagiota","family":"Spyropoulou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliki","family":"Markopoulou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleni","family":"Katsorida","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paraskevi","family":"Stathakopoulou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantina","family":"Filioti","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Markopoulos","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantina","family":"Tsitsa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charalampos","family":"Potsios","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University General Hospital of Patras, 26504 Rio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantinos","family":"Letsas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4760-4165","authenticated-orcid":false,"given":"Panagiota","family":"Xaplanteri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, General Hospital of Eastern Achaia, 25100 Aigio, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lamy, B., Darg\u00e8re, S., Arendrup, M.C., Parienti, J.-J., and Tattevin, P. 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