{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T01:19:42Z","timestamp":1789780782863,"version":"4.0.1"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007904","name":"Texas A&amp;M University","doi-asserted-by":"publisher","award":["Start-up funds to Haitham Mohammed (PI), No. 02-240910-41003"],"award-info":[{"award-number":["Start-up funds to Haitham Mohammed (PI), No. 02-240910-41003"]}],"id":[{"id":"10.13039\/100007904","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>During the summer of 2023, a spontaneous disease outbreak occurred in intensively stocked hybrid catfish (\u2640 channel catfish, Ictalurus punctatus \u00d7 \u2642 blue catfish, I. furcatus) in earthen ponds on a commercial aquaculture farm in South Texas. The farmer reported 50 to 80 dead fish per pond daily for a month. The fish were market size (1.0 \u00b1 0.3 kg on average), resulting in substantial economic losses. Fifteen moribund fish were submitted for laboratory examination. Grossly, the fish showed distended abdomens, erythematous fins, and inflamed vents. Autopsy demonstrated visceral congestion, distended gastrointestinal tracts, and serosanguineous peritoneal effusion. Bacterial cultures from the internal organs revealed homogeneous bacterial growth after incubation. Presumptive biochemical characterization of the isolated bacteria identified Plesiomonas shigelloides. Further molecular confirmation was achieved by species-specific PCR amplification and 16S-rRNA sequencing. Juvenile catfish were experimentally challenged with the recovered isolates to fulfill Koch\u2019s postulates. Moreover, an antibiogram was performed to evaluate the susceptibility of the isolates to a panel of FDA-approved antimicrobials. P. shigelloides isolates were pathogenic to channel catfish and alarmingly multidrug-resistant. We report here, for the first time, P. shigelloides infection in Texas commercial catfish aquaculture, emphasizing its significance as an emerging enteric pathogen that is difficult to treat with FDA-approved antimicrobials.<\/jats:p>","DOI":"10.3390\/microorganisms14010144","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T14:32:38Z","timestamp":1767882758000},"page":"144","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Plesiomonas shigelloides as an Emerging Pathogen in Catfish Aquaculture: A Case from a South Texas Commercial Farm"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0717-6055","authenticated-orcid":false,"given":"Haitham H.","family":"Mohammed","sequence":"first","affiliation":[{"name":"Department of Rangeland, Wildlife and Fisheries Management, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8163-1282","authenticated-orcid":false,"given":"Noha I.","family":"ElBanna","sequence":"additional","affiliation":[{"name":"Department of Rangeland, Wildlife and Fisheries Management, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9348-0581","authenticated-orcid":false,"given":"Ozgur","family":"Erdogan","sequence":"additional","affiliation":[{"name":"Department of Rangeland, Wildlife and Fisheries Management, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suja","family":"Aarattuthodi","sequence":"additional","affiliation":[{"name":"Plant Genetics Research Unit, United States Department of Agriculture\u2014Agricultural Research Service, Columbia, MO 65211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7678-791X","authenticated-orcid":false,"given":"Hasan C.","family":"Tekedar","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Mississippi State University, Starkville, MS 39762, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossam","family":"Abdelhamed","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Mississippi State University, Starkville, MS 39762, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu\u00e9","family":"D\u00edaz-Delgado","sequence":"additional","affiliation":[{"name":"Texas A&M Veterinary Medical Diagnostic Laboratory, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"ref_1","unstructured":"Hanson, T., and Sites, D. (2013). 2012 U.S. Catfish Database, Alabama Agricultural Experiment Station, Fisheries and Allied Aquacultures Department, Auburn University. Available online: http:\/\/aurora.auburn.edu\/bitstream\/handle\/11200\/44174\/2012%20Catfish%20Database.pdf?sequence=."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1111\/jwas.12869","article-title":"Establishment and characterization of a cell line from ictalurid catfish","volume":"53","author":"Aarattuthodi","year":"2022","journal-title":"J. World. Aquacult. Soc."},{"key":"ref_3","unstructured":"USDA NASS (2025, December 08). United States Department Of Agriculture (USDA), Released 10 February 2025, by the National Agricultural Statistics Service (NASS). Available online: https:\/\/downloads.usda.library.cornell.edu\/usda-esmis\/files\/bg257f046\/5999q0580\/kw52m566d\/cfpd0225.pdf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/naaq.10090","article-title":"Direct economic impact of fish diseases on the East Mississippi catfish industry","volume":"81","author":"Peterman","year":"2019","journal-title":"N. Am. J. Aquacult."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1577\/1548-8667(2002)014<0263:TEOBDI>2.0.CO;2","article-title":"The Epidemiology of Bacterial Diseases in Food-Size Channel Catfish","volume":"14","author":"Wagner","year":"2002","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1111\/jfd.12827","article-title":"Winter kill in intensively stocked channel catfish (Ictalurus punctatus): Coinfection with Aeromonas veronii, Streptococcus parauberis and Shewanella putrefaciens","volume":"41","author":"Mohammed","year":"2018","journal-title":"J. Fish Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1111\/jwas.12693","article-title":"An orally delivered, live-attenuated Edwardsiella ictaluri vaccine efficiently protects channel catfish fingerlings against multiple Edwardsiella ictaluri field isolates","volume":"51","author":"Aarattuthodiyil","year":"2020","journal-title":"J. World Aquacult. Soc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2441506","DOI":"10.1080\/29932181.2024.2441506","article-title":"Bridging Aquatic Organism Health and Economics in the Analysis of Disease Impacts and Biosecurity Strategies in Aquaculture: A Conceptual Framework","volume":"1","author":"Brugere","year":"2025","journal-title":"Crit. Insights Aquac."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"739206","DOI":"10.1016\/j.aquaculture.2022.739206","article-title":"Epidemiology and economic impact of disease-related losses on commercial catfish farms: A seven-year case study from Alabama, USA","volume":"566","author":"Abdelrahman","year":"2023","journal-title":"Aquaculture"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1128\/CMR.00103-15","article-title":"Plesiomonas shigelloides Revisited","volume":"29","author":"Janda","year":"2016","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1046\/j.1365-2672.2002.01673.x","article-title":"Bacterial Flora from the Gut of Wild and Cultured Banana Prawn","volume":"93","author":"Oxley","year":"2002","journal-title":"J. Appl. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1046\/j.1365-2672.2003.02109.x","article-title":"Phylogenetic Analysis and In Situ Identification of the Intestinal Microbial Community of Rainbow Trout (Oncorhynchus mykiss, Walbaum)","volume":"96","author":"Huber","year":"2004","journal-title":"J. Appl. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/0044-8486(91)90241-X","article-title":"Antibiotic Resistance in Gram-Negative Bacteria from Cultured Catfish","volume":"99","author":"McPhearson","year":"1991","journal-title":"Aquaculture"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bledsoe, J.W., Peterson, B.C., Swanson, K.S., and Small, B.C. (2016). Ontogenetic Characterization of the Intestinal Microbiota of Channel Catfish through 16S rRNA Gene Sequencing. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0166379"},{"key":"ref_15","first-page":"275","article-title":"Two Epidemics of Diarrhoeal Disease Possibly Caused by Plesiomonas shigelloides","volume":"80","author":"Tsukamoto","year":"1978","journal-title":"Epidemiol. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2675","DOI":"10.1128\/jcm.26.12.2675-2677.1988","article-title":"Pseudoappendicitis Caused by Plesiomonas shigelloides","volume":"26","author":"Fischer","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0163-4453(89)90809-8","article-title":"Plesiomonas shigelloides Meningitis and Septicaemia in a Neonate: Report of a Case and Review of the Literature","volume":"19","author":"Billiet","year":"1989","journal-title":"J. Infect."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0934-8859(99)80003-0","article-title":"Cell Adhesion of Plesiomonas shigelloides","volume":"202","author":"Schubert","year":"1999","journal-title":"Zentralbl. Hyg. Umweltmed."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1128\/JCM.02651-15","article-title":"The Brief Case: Wound Infection with Plesiomonas shigelloides Following a Freshwater Injury","volume":"54","author":"Pence","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1097\/IPC.0000000000000587","article-title":"Plesiomonas shigelloides Gastroenteritis from Freshwater Ingestion in Western Pennsylvania","volume":"26","author":"Peyko","year":"2018","journal-title":"Infect. Dis. Clin. Pract."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/0168-1605(91)90144-E","article-title":"Aeromonas and Plesiomonas as Food- and Waterborne Pathogens","volume":"12","author":"Ljungh","year":"1991","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Meng, S., Xu, J., Xiong, Y., and Ye, C. (2012). Rapid and Sensitive Detection of Plesiomonas shigelloides by Loop-Mediated Isothermal Amplification. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0041978"},{"key":"ref_23","unstructured":"Thoesen, J.C. (2004). Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens, Fish Health Section, American Fisheries Society."},{"key":"ref_24","unstructured":"USFWS, and AFS-FHS (2020). FHS Blue Book: Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens, American Fisheries Society-Fish Health Section. Available online: https:\/\/units.fisheries.org\/fhs\/fish-health-section-blue-book-2020\/."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1128\/JB.183.9.2715-2723.2001","article-title":"Characterization of Plesiomonas shigelloides Genes Encoding the Heme Iron Utilization System","volume":"183","author":"Henderson","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.ijfoodmicro.2005.12.008","article-title":"Occurrence of Plesiomonas shigelloides in Saltwater Fish Determined by PCR Assay","volume":"108","author":"Herrera","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1111\/jam.12475","article-title":"Characterization of the Gut Microbiota of Three Warmwater Fish Species","volume":"116","author":"Larsen","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2017.09.019","article-title":"Mechanisms of Pathogen Virulence and Host Susceptibility in Aeromonas hydrophila Infections of Channel Catfish","volume":"482","author":"Peatman","year":"2018","journal-title":"Aquaculture"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/15222055.2011.544944","article-title":"Preparation of Ormetoprim\u2013Sulfadimethoxine-Medicated Discs for Disc Diffusion Assay","volume":"73","author":"Gaunt","year":"2011","journal-title":"N. Am. J. Aquac."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/jfd.12402","article-title":"Protective Efficacy of Nigella sativa Seeds and Oil against Columnaris Disease in Fishes","volume":"39","author":"Mohammed","year":"2016","journal-title":"J. Fish Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3354\/dao02739","article-title":"Epidemiology of Columnaris Disease Affecting Fishes within the Same Watershed","volume":"109","author":"Mohammed","year":"2014","journal-title":"Dis. Aquat. Org."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1046\/j.1365-2761.1999.00134.x","article-title":"Histopathology in Fish: Proposal for a Protocol to Assess Aquatic Pollution","volume":"22","author":"Bernet","year":"1999","journal-title":"J. Fish Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.1111\/raq.12435","article-title":"A Proposal for Standardization of Histopathological Lesions to Characterize Fish Diseases","volume":"12","author":"Rey","year":"2020","journal-title":"Rev. Aquac."},{"key":"ref_34","unstructured":"Pillay, T.V.R., and Kutty, M.N. (2005). Aquaculture: Principles and Practices, Wiley-Blackwell. [2nd ed.]."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/1365-2644.12017","article-title":"More Rapid and Severe Disease Outbreaks for Aquaculture in the Tropics","volume":"50","author":"Leung","year":"2013","journal-title":"J. Appl. Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jare.2014.10.009","article-title":"Emerging Flavobacterial Infections in Fish: A Review","volume":"6","author":"Loch","year":"2015","journal-title":"J. Adv. Res."},{"key":"ref_37","first-page":"1","article-title":"Plesiomonas shigelloides, a Potential Pathogen of Enteritis in Ictalurus punctatus","volume":"72","author":"Wang","year":"2020","journal-title":"Isr. J. Aquac."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e70039","DOI":"10.1111\/raq.70039","article-title":"The Current State of Knowledge of the Economic Impact of Diseases in Global Aquaculture","volume":"17","author":"Maezono","year":"2025","journal-title":"Rev. Aquac."},{"key":"ref_39","first-page":"10","article-title":"Complete Genome Sequence of Multidrug-Resistant Plesiomonas shigelloides Strain MS-17-188","volume":"6","author":"Abdelhamed","year":"2018","journal-title":"Genome Announc."},{"key":"ref_40","first-page":"96","article-title":"Identification and Pathological Observation of a Pathogenic Plesiomonas shigelloides Strain from Tilapia","volume":"55","author":"Liu","year":"2015","journal-title":"Acta Microbiol. Sin."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"100441","DOI":"10.1016\/j.microb.2025.100441","article-title":"Genomic Islands and Mobilome Demonstrate a Potential Pathogen, Plesiomonas shigelloides, in Nile Tilapia (Oreochromis niloticus)","volume":"8","author":"Aburjaile","year":"2025","journal-title":"Microbe"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.aquaculture.2018.04.063","article-title":"Identification and Pathogenicity of Plesiomonas shigelloides in Silver Carp","volume":"493","author":"Behera","year":"2018","journal-title":"Aquaculture"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Chen, H., Zhao, Y., Chen, K., Wei, Y., Luo, H., Li, Y., Liu, F., Zhu, Z., Hu, W., and Luo, D. (2022). Isolation, Identification, and Investigation of Pathogenic Bacteria from Common Carp Naturally Infected with Plesiomonas shigelloides. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.872896"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"102414","DOI":"10.1016\/j.aqrep.2024.102414","article-title":"Identification and Characteristics of Plesiomonas shigelloides Isolated from Mastacembelus armatus","volume":"39","author":"Yu","year":"2024","journal-title":"Aquac. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Wang, Z., Gu, S., Lv, W., Chen, J., Xue, M., Liu, S., Mao, J., and Chen, G. (2025). Genomic and Transcriptomic Profiling of a Highly Virulent Plesiomonas shigelloides Strain. Microorganisms, 13.","DOI":"10.3390\/microorganisms13092168"},{"key":"ref_46","first-page":"617","article-title":"Aquarium-Associated Plesiomonas shigelloides Infection\u2014Missouri","volume":"38","author":"Tippen","year":"1989","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_47","unstructured":"Mohammed, H.H., and Peatman, E. (2018, January 9\u201313). Acute Summer Gastroenteritis of Intensively Stocked Hybrid Catfish. Proceedings of the 43rd Eastern Fish Health Workshop, Chattanooga, TN, USA."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1099\/00222615-13-3-401","article-title":"Enteropathogenicity of Plesiomonas shigelloides","volume":"13","author":"Sanyal","year":"1980","journal-title":"J. Med. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1093\/clinids\/10.2.303","article-title":"Clinical Disease Spectrum and Pathogenic Factors Associated with Plesiomonas shigelloides Infections in Humans","volume":"10","author":"Brenden","year":"1988","journal-title":"Clin. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Ekundayo, T.C., and Okoh, A.I. (2018). Pathogenomics of Virulence Traits of Plesiomonas shigelloides. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.03077"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1053\/j.semdp.2019.04.012","article-title":"Emerging Agents of Gastroenteritis: Aeromonas, Plesiomonas, and Diarrheagenic E. coli","volume":"36","author":"Schuetz","year":"2019","journal-title":"Semin. Diagn. Pathol."},{"key":"ref_52","first-page":"3119958","article-title":"Plesiomonas: A Review on Food Safety, Fish-Borne Diseases, and Tilapia","volume":"2021","year":"2021","journal-title":"Sci. World J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"dlae109","DOI":"10.1093\/jacamr\/dlae109","article-title":"Comparative Analysis of Three Plasmids from Plesiomonas shigelloides Strain MS-17-188 and Their Role in Antimicrobial Resistance","volume":"6","author":"Abdelhamed","year":"2024","journal-title":"JAC-Antimicrob. Resist."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3354\/dao02492","article-title":"Catfish Hybrid Ictalurus punctatus \u00d7 I. furcatus Exhibits Higher Resistance to Columnaris Disease than the Parental Species","volume":"100","author":"Arias","year":"2012","journal-title":"Dis. Aquat. Org."},{"key":"ref_55","unstructured":"Dunham, R.A., and Masser, M.P. (2012). Production of Hybrid Catfish, Southern Regional Aquaculture Center."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1577\/1548-8659(1983)45[55:RTOCXB]2.0.CO;2","article-title":"Relative Tolerance of Channel \u00d7 Blue Hybrid and Channel Catfish to Low Oxygen Concentrations","volume":"45","author":"Dunham","year":"1983","journal-title":"Prog. Fish-Cult."},{"key":"ref_57","first-page":"375","article-title":"Plesiomonas shigelloides Infection in Hong Kong: Retrospective Study of 167 Cases","volume":"6","author":"Wong","year":"2000","journal-title":"Hong Kong Med. J."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Reischl, U., Wittwer, C., and Cockerill, F. (2002). Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR. Rapid Cycle Real-Time PCR\u2014Methods and Applications: Microbiology and Food Analysis, Springer.","DOI":"10.1007\/978-3-642-48351-6"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/1\/144\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T05:14:12Z","timestamp":1768367652000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/1\/144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["microorganisms14010144"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14010144","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]}}}