{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:22:00Z","timestamp":1780327320944,"version":"3.54.1"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Municipal wastewater contains high amounts of nitrogen (N) and phosphorus (P), as well as other compounds that are harmful to the environment; however, it can also be used as an algae growth medium. In this study locally (Lithuania) isolated algae Scenedesmus quadricauda were cultivated in local (Vilnius city) municipal wastewater. Data show that Scenedesmus algae can be grown in municipal wastewater as successfully as in Bold\u2019s basal medium for 14 days. Algae cultivation significantly reduced the concentration of organic nitrogen forms and phosphate levels. The nitrogen concentration in wastewater after cultivation was reduced to 8 mg N L\u22121 (up to 89% reduction in total nitrogen concentration). Phosphorus concentration was reduced to 5.4 mg P L\u22121 (up to 86%). The analysis indicates that the optimal temperature for S. quadricauda cultivation is 25 \u00b0C; temperatures higher or lower than this result in a reduction in algal biomass. A higher amount of light leads to higher yields. No statistically significant differences were found comparing cultivation in BB medium and wastewater under different conditions. The analysis showed that the main factors influencing algae biochemical composition were final total nitrogen concentration and available total nitrogen amount per unit of algae biomass produced, as well as molar N:P ratios. Algae biomass cultivated in wastewater contained a consistent lipid concentration (on average 14.94 \u00b1 2.38%), a lower final total nitrogen concentration, and overall lower total nitrogen availability, leading to higher carbohydrate concentrations (up to 51.10%) and a lower protein content (down to 15.52%). Algae biomass that was cultivated in the BB medium biochemical composition was not dependent on environmental factors and remained consistent (on average 22.89 \u00b1 3.85% carbohydrate, 39.32 \u00b1 3.89% protein, and 13.99 \u00b1 2.21% lipid).<\/jats:p>","DOI":"10.3390\/microorganisms14010183","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T11:01:14Z","timestamp":1768388474000},"page":"183","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Influence of Light, Temperature, and Nutrient Availability on Growth and Biochemical Composition of Scenedesmus quadricauda Cultivated in Municipal Wastewater"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3510-7241","authenticated-orcid":false,"given":"Petras","family":"Venckus","sequence":"first","affiliation":[{"name":"Life Sciences Center, Institute of Biosciences, Vilnius University, Saul\u0117tekis Ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6390-9033","authenticated-orcid":false,"given":"Egl\u0117","family":"Lastauskien\u0117","sequence":"additional","affiliation":[{"name":"Life Sciences Center, Institute of Biosciences, Vilnius University, Saul\u0117tekis Ave. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.biortech.2018.01.012","article-title":"Tofu Whey Wastewater Is a Promising Basal Medium for Microalgae Culture","volume":"253","author":"Wang","year":"2018","journal-title":"Bioresour. 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