{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:37Z","timestamp":1787028997639,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-64-00017"],"award-info":[{"award-number":["24-64-00017"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The paper describes a predatory Gram-negative bacterium from the genus Bdellovibrio, which was isolated from water of the Lyubozhikha River. As revealed by electron microscopy, the bacterium is an intracellular predator of Gram-negative microorganisms. Its prey range includes Pseudomonas tolaasii, the phytopathogen responsible for brown spot disease in the cultivated button mushroom (Agaricus bisporus). Based on the results of a 16S rRNA gene sequence analysis, the bacterium was identified as Bdellovibrio bacteriovorus strain LR3. We characterized the predator\u2013prey dynamics between B. bacteriovorus LR3 and P. tolaasii, determining the optimal temperature and pH conditions for this interaction. Our results demonstrate the potential of B. bacteriovorus LR3 as a biocontrol agent against P. tolaasii in mushroom cultivation. The possibility of using B. bacteriovorus LR3 against clinical cases Salmonella and Escherichia infections is also addressed.<\/jats:p>","DOI":"10.3390\/microorganisms14010190","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:26:36Z","timestamp":1768465596000},"page":"190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Predatory Bacteria Bdellovibrio bacteriovorus LR3: A Potential Biocontrol Agent Against Gram-Negative Pathogenic Microorganisms"],"prefix":"10.3390","volume":"14","author":[{"given":"Anna P.","family":"Shorokhova","sequence":"first","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6960-3967","authenticated-orcid":false,"given":"Valentina N.","family":"Polivtseva","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4539-5192","authenticated-orcid":false,"given":"Tatiana N.","family":"Abashina","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4166-3105","authenticated-orcid":false,"given":"Vladimir V.","family":"Sorokin","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey V.","family":"Chekanov","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander S.","family":"Reshetnikov","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Bogun","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanina A.","family":"Delegan","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"},{"name":"D.I. Ivanovsky\u2019s Academy of Biology and Biotechnology, Southern Federal University, 344006 Rostov-on-Don, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei A.","family":"Zimin","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4960-1910","authenticated-orcid":false,"given":"Nataliya E.","family":"Suzina","sequence":"additional","affiliation":[{"name":"G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Karwowska, E. (2024). Antibiotic Resistance in the Farming Environment. Appl. Sci., 14.","DOI":"10.3390\/app14135776"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. 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