{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T17:16:32Z","timestamp":1768670192221,"version":"3.49.0"},"reference-count":64,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Higher Education","award":["FRGS\/1\/2020\/SKK0\/UPM\/01\/2"],"award-info":[{"award-number":["FRGS\/1\/2020\/SKK0\/UPM\/01\/2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Fusidic acid resistance in Staphylococcus spp. has historically been confined to Staphylococcus ureilyticus, with limited data on its environmental distribution. This study presents the first detection of the fusidic acid resistance gene fusF in Staphylococcus kloosii recovered from virgin soil at Kampung Batu 16, Dusun Tua, Hulu Langat, Malaysia. A total of ten Staphylococcus isolates were identified using the VITEK\u00ae2 system with high confidence (97\u201399%), comprising seven S. kloosii and three S. ureilyticus. Sequencing of representative isolates further corroborated the species identification. All isolates displayed phenotypic resistance to fusidic acid, while all S. ureilyticus (3\/3) exhibited multi-drug resistant (MDR) traits and S. kloosii (7\/7) exhibited non-MDR traits. PCR and sequencing confirmed the presence of fusF gene in S. ureilyticus (3\/3) and S. kloosii (3\/7). In addition, fusB and fusC genes were not detected in both species. The phylogenetic analysis (Maximum Likelihood, Tamura\u2013Nei model) revealed high sequence conservation and clustering between fusF-positive S. kloosii and S. ureilyticus soil isolates, suggesting recent horizontal gene transfer between these two related species. The first detection of fusF gene in S. kloosii from virgin soil signifies the expansion of the ecological and host range beyond S. ureilyticus, establishes virgin soil as a potential antimicrobial resistance (AMR) reservoir, and underscores the One Health risks of resistance dissemination from environmental staphylococci. This baseline study highlights the importance of early AMR surveillance in tropical environments prior to agricultural development.<\/jats:p>","DOI":"10.3390\/microorganisms14010197","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:13:21Z","timestamp":1768475601000},"page":"197","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Report of fusF Gene in Staphylococcus kloosii from Virgin Tropical Soil: Expanding the Ecological Reservoirs of Fusidic Acid Resistance"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0142-4753","authenticated-orcid":false,"given":"Muhammad Haziq","family":"Ruzaini Abdullah","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7900-9678","authenticated-orcid":false,"given":"Mohd Huzairi Mohd","family":"Zainudin","sequence":"additional","affiliation":[{"name":"Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musheer A.","family":"Aljaberi","sequence":"additional","affiliation":[{"name":"Department of Science, Franciscus Gasthuis & Vlietland, 3045 PM Rotterdam, The Netherlands"},{"name":"Research Centre Innovations in Care, Rotterdam University of Applied Sciences, 3015 EK Rotterdam, The Netherlands"},{"name":"Department of Internal Medicine, Section Nursing Science, Erasmus University Medical Center (Erasmus MC), 3015 GD Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor Azira","family":"Binti Abdul Mutalib","sequence":"additional","affiliation":[{"name":"Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-min","family":"Neoh","sequence":"additional","affiliation":[{"name":"UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6288-3791","authenticated-orcid":false,"given":"Rukman Awang","family":"Hamat","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00313-18","DOI":"10.1128\/mSystems.00313-18","article-title":"Core Microbiota in Agricultural Soils and Their Potential Associations with Nutrient Cycling","volume":"4","author":"Jiao","year":"2019","journal-title":"mSystems"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/s43247-024-01767-z","article-title":"Soil microbial biodiversity supports the delivery of multiple ecosystem functions under elevated CO2 and warming","volume":"5","author":"Wang","year":"2024","journal-title":"Commun. 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