{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:27:54Z","timestamp":1781821674708,"version":"3.54.5"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2023YFD1301800"],"award-info":[{"award-number":["2023YFD1301800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Plan Program of Yunfu city","award":["YF2025NYRC03"],"award-info":[{"award-number":["YF2025NYRC03"]}]},{"name":"Science and Technology Plan Program of Yunfu city","award":["2024090301"],"award-info":[{"award-number":["2024090301"]}]},{"name":"Science and Technology Plan Program of Yunfu city","award":["2024020101"],"award-info":[{"award-number":["2024020101"]}]},{"name":"Science and Technology Plan Program of Guangdong Province","award":["2023B1212070018"],"award-info":[{"award-number":["2023B1212070018"]}]},{"name":"Fourth Round of Guangdong Provincial Modern Agricultural Industry Technology System Innovation Team Construction Project","award":["2024CXTD15"],"award-info":[{"award-number":["2024CXTD15"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Mycoplasma synoviae (MS) is a major pathogen threatening China\u2019s poultry industry, causing severe economic losses, and clarifying its epidemiology is pivotal for disease control and flock purification. In this study, a total of 3215 chicken samples collected from 643 broiler farms across 15 provinces in China in 2024 were analyzed. PCR detected 14% positivity (450 samples), and 18 isolates obtained from these positive samples (4.0% isolation rate). Multilocus sequence typing (MLST, 7 housekeeping genes) and neighbor-joining phylogenetic analysis (integrating 425 reference sequences from public databases) identified 13 distinct sequence types (STs), demonstrating considerable genetic diversity among circulating MS strains. Pathogenicity assessment of the five isolates revealed that the infected chickens exhibited varying degrees of infectious synovitis, while no respiratory signs were observed. In addition, antimicrobial susceptibility testing against 10 commonly used antibiotics was conducted on the 18 strains, providing urgently needed guidance for rational drug use in the clinical treatment of both breeder and broiler flocks. This large-scale epidemiological study yields crucial insights into the current prevalence and genetic diversity of MS in China and lays a scientific foundation for formulating targeted prevention strategies and optimizing management practices.<\/jats:p>","DOI":"10.3390\/microorganisms14010257","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T13:55:34Z","timestamp":1769176534000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic Diversity and Emerging Trends of Mycoplasma synoviae in China: Insights from a 2024 Nationwide MLST Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Lu","family":"Tu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Liu","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Jin","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Yin","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Wang","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Yantai Animal Disease Prevention and Control Center, Yantai 264000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Xinxing, Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2293-0287","authenticated-orcid":false,"given":"Youzhi","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.1111\/tbed.14302","article-title":"Pooled molecular occurrence of Mycoplasma gallisepticum and Mycoplasma synoviae in poultry: A systematic review and meta-analysis","volume":"69","author":"Velazquez","year":"2022","journal-title":"Transbound. 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