{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:56:46Z","timestamp":1791824206923,"version":"4.3.4"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"crossref","award":["PI21CIII\/00012"],"award-info":[{"award-number":["PI21CIII\/00012"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Center for Biomedical Research in Network in Infectious Diseases","award":["CB21\/13\/00105"],"award-info":[{"award-number":["CB21\/13\/00105"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>One of the most notable aspects of Aspergillus fumigatus, and related to its dynamic adaptation, is its ability to form biofilm and produce a wide variety of secondary metabolites. The aim of this study is to advance the characterization of biofilms generated by different A. fumigatus strains across their developmental stages and analytically evaluate their structure and composition and their relationship with secondary metabolism activation. An in vitro biofilm model was standardized to investigate structural and analytical differences among strains isolated from distinct clinical settings and associated with different pathologies. We found that all tested strains could form biofilms; however, the characteristics of these structures\u2014including total biomass, cellular viability and overall structure\u2014varied markedly among strains under the evaluated conditions. Strains isolated from cystic fibrosis patients exhibited distinct behaviors in most conducted assays compared to other strains. These findings provide new insights into the variability of biofilm composition and may contribute to a better understanding of the role of biofilms in fungal pathogenesis, persistence and treatment resistance.<\/jats:p>","DOI":"10.3390\/microorganisms14020272","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T10:24:36Z","timestamp":1769423076000},"page":"272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biofilm Formation in Aspergillus fumigatus: A Comparative Study of Strains from Different Origins"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6465-0807","authenticated-orcid":false,"given":"Marta","family":"Cano-P\u00e9rez","sequence":"first","affiliation":[{"name":"Mycology Reference and Research Laboratory, National Centre for Microbiology-Instituto de Salud Carlos III (CNM-ISCIII), Carretera Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan de Dios","family":"Caballero P\u00e9rez","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Hospital Ramon y Cajal, 28034 Madrid, Spain"},{"name":"Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), 28034 Madrid, Spain"},{"name":"Centre for Biomedical Research in Network in Infectious Diseases (CIBERINFEC), CB21\/13\/00105, ISCIII, 28220 Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0986-3940","authenticated-orcid":false,"given":"Elia","family":"G\u00f3mez Garc\u00eda de la Pedrosa","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Hospital Ramon y Cajal, 28034 Madrid, Spain"},{"name":"Instituto Ram\u00f3n y Cajal de Investigaci\u00f3n Sanitaria (IRYCIS), 28034 Madrid, Spain"},{"name":"Centre for Biomedical Research in Network in Infectious Diseases (CIBERINFEC), CB21\/13\/00105, ISCIII, 28220 Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2780-5039","authenticated-orcid":false,"given":"Alicia","family":"G\u00f3mez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Mycology Reference and Research Laboratory, National Centre for Microbiology-Instituto de Salud Carlos III (CNM-ISCIII), Carretera Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain"},{"name":"Centre for Biomedical Research in Network in Infectious Diseases (CIBERINFEC), CB21\/13\/00105, ISCIII, 28220 Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.riam.2021.03.003","article-title":"Invasive fungal infection over the last 30 years","volume":"38","author":"Silva","year":"2021","journal-title":"Rev. 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