{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:08:14Z","timestamp":1781539694790,"version":"3.54.5"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014103","name":"Key Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2022TZXD0037"],"award-info":[{"award-number":["2022TZXD0037"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Calcareous soils are typically deficient in essential nutrients such as iron, phosphorus, and potassium, which frequently results in nutrient deficiency in fruit trees. Bacillus licheniformis LCDD6 markedly enhanced Malus hupehensis seedling growth and plant iron nutrition in calcareous soil. This study aimed to elucidate the mechanism underlying these beneficial effects of strain LCDD6 under iron deficiency. Transcriptomic analysis revealed that iron deficiency induced metabolic reprogramming in strain LCDD6, characterized by a significant upregulation of genes involved in the biosynthesis of the siderophore bacillibactin and plant growth hormone indoleacetic acid (IAA). Consistently, metabolomic profiling identified bacillibactin and IAA as the dominant metabolites produced under iron-deficient conditions. A 60-day pot experiment further demonstrated that the cell-free fermentation broth of strain LCDD6 significantly enhanced plant growth and rhizosphere soil enzyme activities. The crude bacillibactin extract derived from the fermentation exerted the strongest effects on plant growth and iron accumulation, whereas IAA preferentially stimulated root development and promoted plant phosphorus accumulation. Additionally, different metabolites exerted distinct and selective effects on the rhizosphere microbial community, with fungi showing stronger and more metabolite-specific responses than bacteria. The crude bacillibactin extract enriched fungal taxa, particularly Coprinellus, which showed strong positive correlations with plant growth traits and iron accumulation, while Stachybotrys, enriched under IAA treatment, was positively correlated with plant phosphorus content. Overall, strain LCDD6 promotes plant growth under iron-deficient conditions through the coordinated action of multiple metabolites, with bacillibactin as the primary contributor and IAA providing complementary effects. These findings offer mechanistic insight and a scientific basis for developing Bacillus-based biofertilizers to improve nutrient acquisition in calcareous soils.<\/jats:p>","DOI":"10.3390\/microorganisms14020349","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:00:58Z","timestamp":1770109258000},"page":"349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Metabolite-Mediated Alleviation of Iron Deficiency and Growth Promotion of Malus hupehensis by Bacillus licheniformis LCDD6 in Calcareous Soil"],"prefix":"10.3390","volume":"14","author":[{"given":"Jie","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6020-0130","authenticated-orcid":false,"given":"Shanshan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3276-9143","authenticated-orcid":false,"given":"Yanqin","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China"},{"name":"Shandong Engineering Research Center of Plant-Microbial Restoration for Saline-Alkali Land, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"ref_1","first-page":"7903796","article-title":"Effects of Foliar Fertilization on Nutrient Uptake, Yield, and Fruit Quality of Pomelo (Citrus grandis Osbeck) Grown in the Mekong Delta Soils","volume":"2022","author":"Hung","year":"2022","journal-title":"Int. J. Agron."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Li, J., Cao, X., Jia, X., Liu, L., Cao, H., Qin, W., and Li, M. (2021). Iron deficiency leads to chlorosis through impacting chlorophyll synthesis and nitrogen metabolism in Areca catechu L.. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.710093"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Li, M., Watanabe, S., Gao, F., and Dubos, C. (2023). Iron nutrition in plants: Towards a new paradigm?. Plants, 12.","DOI":"10.3390\/plants12020384"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s10725-023-00979-1","article-title":"Iron transport and homeostasis in plants: Current updates and applications for improving human nutrition values and sustainable agriculture","volume":"100","author":"Khan","year":"2023","journal-title":"Plant Growth Regul."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100008","DOI":"10.1016\/j.stress.2021.100008","article-title":"Iron homeostasis in plants and its crosstalk with copper, zinc, and manganese","volume":"1","author":"Rai","year":"2021","journal-title":"Plant Stress"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"20621","DOI":"10.1038\/s41598-023-47924-w","article-title":"Diazotrophic Azotobacter salinestris YRNF3: A probable calcite-solubilizing bio-agent for improving the calcareous soil properties","volume":"13","author":"Rashad","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/bs.agron.2023.06.002","article-title":"Chapter two\u2014Distribution, characteristics and management of calcareous soils","volume":"182","author":"Bolan","year":"2023","journal-title":"Adv. Agron."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107321","DOI":"10.1016\/j.agwat.2021.107321","article-title":"A global meta-analysis of fruit tree yield and water use efficiency under deficit irrigation","volume":"260","author":"Tong","year":"2022","journal-title":"Agric. Water Manag."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Song, J., Han, M., Zhu, X., Li, H., Ning, Y., Zhang, W., and Yang, H. (2023). MhCLC-c1, a Cl channel c homolog from Malus hupehensis, alleviates NaCl-induced cell death by inhibiting intracellular Cl\u2212 accumulation. BMC Plant Biol., 23.","DOI":"10.1186\/s12870-023-04270-3"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.plaphy.2020.09.009","article-title":"H2S pretreatment mitigates the alkaline salt stress on Malus hupehensis roots by regulating Na+\/K+ homeostasis and oxidative stress","volume":"156","author":"Li","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_11","first-page":"17","article-title":"Iron nutrition management in calcisol soils as a tool to mitigate chlorosis and promote crop quality\u2014An overview","volume":"12","author":"Acosta","year":"2024","journal-title":"J. Appl. Biol. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Pandey, S.S. (2023). The role of iron in phytopathogenic microbe\u2013plant interactions: Insights into virulence and host immune response. Plants, 12.","DOI":"10.3390\/plants12173173"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pecoraro, L., Wang, X., Shah, D., Song, X., Kumar, V., Shakoor, A., Tripathi, K., Ramteke, P.W., and Rani, R. (2021). Biosynthesis pathways, transport mechanisms and biotechnological applications of fungal siderophores. J. Fungi, 8.","DOI":"10.3390\/jof8010021"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41579-024-01090-6","article-title":"Bacterial siderophores: Diversity, uptake pathways and applications","volume":"23","author":"Schalk","year":"2025","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"116481","DOI":"10.1016\/j.celrep.2025.116481","article-title":"Siderophore-mediated iron enrichment in the biofilm matrix enhances plant iron nutrition","volume":"44","author":"Tan","year":"2025","journal-title":"Cell Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1186\/2193-1801-2-587","article-title":"Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils","volume":"2","author":"Sharma","year":"2013","journal-title":"Springerplus"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sun, W., Shahrajabian, M.H., and Soleymani, A. (2024). The roles of plant-growth-promoting rhizobacteria (PGPR)-based biostimulants for agricultural production systems. Plants, 13.","DOI":"10.3390\/plants13050613"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1007\/s00284-024-03749-y","article-title":"Bacillus licheniformis and Bacillus velezensis from rhizosphere of Clerodendrum infortunatum L. promote plant growth and resistance to Sclerotium rolfsii in Vigna unguiculata (L.) Walp","volume":"81","author":"Panichikkal","year":"2024","journal-title":"Curr. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e2500016","DOI":"10.1002\/jobm.202500016","article-title":"Stress-induced response and adaptation mechanisms in Bacillus licheniformis PSKA1 exposed with abiotic and antibiotic stresses","volume":"65","author":"Kadapure","year":"2025","journal-title":"J. Basic Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s13213-025-01797-8","article-title":"Whole-genome sequencing uncovers the plant growth-promoting potential of Bacillus licheniformis G41, isolated from the rhizosphere soil of Gannan navel orange","volume":"75","author":"Cao","year":"2025","journal-title":"Ann. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ni, S., Wu, Y., Zhu, N., Leng, F., and Wang, Y. (2024). Bacillus licheniformis YB06: A rhizosphere-genome-wide analysis and plant growth-promoting analysis of a plant growth-promoting rhizobacterium isolated from Codonopsis pilosula. Microorganisms, 12.","DOI":"10.3390\/microorganisms12091861"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e2300643","DOI":"10.1002\/jobm.202300643","article-title":"Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant","volume":"64","author":"Alarjani","year":"2024","journal-title":"J. Basic Microbiol."},{"key":"ref_23","first-page":"588","article-title":"Genome-wide association analysis of the growth-promoting effects of Bacillus licheniformis LCDD6 on walnut seedlings","volume":"28","author":"Bing","year":"2022","journal-title":"Chin. J. Appl. Environ. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"200","DOI":"10.3844\/ajbbsp.2018.200.209","article-title":"Mining, isolation and identification of siderophore synthesis gene from Brevibacillus brevis GZDF3","volume":"14","author":"Sheng","year":"2018","journal-title":"Am. J. Biochem. Biotechnol."},{"key":"ref_25","unstructured":"Bao, S. (2000). Soil and Agricultural Chemistry Analysis, China Agriculture Press. [3rd ed.]."},{"key":"ref_26","first-page":"71","article-title":"Effects of iron on crops and availability of iron in soil: A review","volume":"15","author":"Porkodi","year":"2023","journal-title":"Biol. Forum Int. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s42398-018-0025-z","article-title":"Plant growth promoting potential of Aspergillus sp. NPF7, isolated from wheat rhizosphere in South Gujarat, India","volume":"1","author":"Pandya","year":"2018","journal-title":"Environ. Sustain."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Nithyapriya, S., Lalitha, S., Sayyed, R.Z., Reddy, M.S., Dailin, D.J., El Enshasy, H.A., Luh Suriani, N., and Herlambang, S. (2021). Production, purification, and characterization of bacillibactin siderophore of Bacillus subtilis and its application for improvement in plant growth and oil content in sesame. Sustainability, 13.","DOI":"10.3390\/su13105394"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102269","DOI":"10.1101\/pdb.prot102269","article-title":"Bradford Assay for Determining Protein Concentration","volume":"4","author":"Kielkopf","year":"2020","journal-title":"Cold Spring Harb. Protoc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ecoenv.2016.11.029","article-title":"Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress","volume":"137","author":"Torabian","year":"2017","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_31","first-page":"1188","article-title":"Atomic Absorption Spectrophotometric Method for the Estimation of Micronutrients in Soil and their Effect on Plant Growth","volume":"18","author":"Singhal","year":"2024","journal-title":"ES Food Agrofor."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kandasamy, S., Dhandayuthapani, U.N., Subramanian, V., Palanisamy, J., Shanmugam, M.K., Dhakshanamoorthy, D., Subramani, U.K., and Nagappan, S. (2025). Assessment of brewery wastewater as an alternative irrigation source: Impacts on soil health and nutrient uptake by maize in Tamil Nadu, India. BMC Agric., 1.","DOI":"10.1186\/s44399-025-00002-0"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Qu, Y., Tang, J., Li, Z., Zhou, Z., Wang, J., Wang, S., and Cao, Y. (2020). Soil Enzyme Activity and Microbial Metabolic Function Diversity in Soda Saline\u2013Alkali Rice Paddy Fields of Northeast China. Sustainability, 12.","DOI":"10.3390\/su122310095"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5815","DOI":"10.1016\/S0040-4039(01)81061-3","article-title":"Smenospongine: A cytotoxic and antimicrobial aminoquinone isolated from Smenospongia sp.","volume":"28","author":"Kondracki","year":"1987","journal-title":"Tetrahedron Lett."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Long, J., Ji, W., Zhang, D., Zhu, Y., and Bi, Y. (2021). Bioactivities and structure-activity relationships of fusidic acid derivatives: A Review. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.759220"},{"key":"ref_36","first-page":"742149","article-title":"Structure and antimicrobial properties of monensin A and its derivatives: Summary of the achievements","volume":"2013","author":"Aowicki","year":"2013","journal-title":"Biomed Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Tsotetsi, T., Nephali, L., Malebe, M., and Tugizimana, F. (2022). Bacillus for Plant Growth Promotion and Stress Resilience: What Have We Learned?. Plants, 11.","DOI":"10.3390\/plants11192482"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e20204","DOI":"10.1016\/j.heliyon.2023.e20204","article-title":"Evaluating the potential of Bacillus licheniformis YZCUO202005 isolated from lichens in maize growth promotion and biocontrol","volume":"9","author":"Medison","year":"2023","journal-title":"Heliyon"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4038","DOI":"10.1093\/jxb\/erab019","article-title":"Organic acids: Versatile stress-response roles in plants","volume":"72","author":"Panchal","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6248","DOI":"10.1021\/jm049655r","article-title":"Acetylcholinesterase inhibitory activity of scopolin and scopoletin discovered by virtual screening of natural products","volume":"47","author":"Rollinger","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"133110","DOI":"10.1016\/j.jclepro.2022.133110","article-title":"Siderophores, a potential phosphate solubilizer from the endophyte Streptomyces sp. CoT10, improved phosphorus mobilization for host plant growth and rhizosphere modulation","volume":"367","author":"Cui","year":"2022","journal-title":"J. Clean. Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/j.1574-6976.2007.00072.x","article-title":"Indole-3-acetic acid in microbial and microorganism-plant signaling","volume":"31","author":"Spaepen","year":"2007","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e16306","DOI":"10.1016\/j.heliyon.2023.e16306","article-title":"IAA and ACC deaminase producing-bacteria isolated from the rhizosphere of pineapple plants grown under different abiotic and biotic stresses","volume":"9","author":"Ratnaningsih","year":"2023","journal-title":"Heliyon"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Oh, S., Cave, G., and Lu, C. (2021). Vitamin B12 (Cobalamin) and Micronutrient Fortification in Food Crops Using Nanoparticle Technology. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.668819"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Shen, Y., Zhao, J., Zou, X., Shi, Z., Liao, Y., He, Y., Wang, H., Chen, Q., Yang, P., and Li, M. (2023). Differential responses of bacterial and fungal communities to siderophore supplementation in soil affected by tobacco bacterial wilt (Ralstonia solanacearum). Mcroorganisms, 11.","DOI":"10.3390\/microorganisms11061535"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"892","DOI":"10.3390\/microbiolres14030062","article-title":"Biocontrol efficiency of rhizospheric Bacillus against the plant pathogen Fusarium oxysporum: A promising approach for sustainable agriculture","volume":"14","author":"Boulahouat","year":"2023","journal-title":"Microbiol. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e026","DOI":"10.48130\/sif-0025-0024","article-title":"Status review of the distribution, biological compounds, and bioactivity of Coprinellus (inky cap mushroom)","volume":"10","author":"Fabros","year":"2025","journal-title":"Stud. Fungi"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Xia, Q., Rufty, T., and Shi, W. (2021). Predominant microbial colonizers in the root endosphere and rhizosphere of turfgrass systems: Pseudomonas veronii, Janthinobacterium lividum, and Pseudogymnoascus spp.. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.643904"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wang, Y., Yang, X., Li, Y., Wang, B., and Shi, T. (2023). The genus Chrysosporium: A potential producer of natural products. Fermentation, 9.","DOI":"10.3390\/fermentation9010076"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Colinas-Picazo, A., Mendoza-de Gives, P., P\u00e9rez-Anz\u00farez, G., Guti\u00e9rrez-Medina, E., Bautista-Garc\u00eda, G.A., Delgado-N\u00fa\u00f1ez, E.J., and Olmedo-Ju\u00e1rez, A. (2024). Assessing the in vitro individual and combined effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) liquid culture filtrates against infective larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae). Pathogens, 13.","DOI":"10.20944\/preprints202404.1103.v1"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ozimek, E., and Hanaka, A. (2021). Mortierella species as the plant growth-promoting fungi present in the agricultural soils. Agriculture, 11.","DOI":"10.3390\/agriculture11010007"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/2\/349\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:18:56Z","timestamp":1770110336000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/2\/349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,3]]},"references-count":51,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["microorganisms14020349"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14020349","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,3]]}}}