{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T01:12:07Z","timestamp":1770167527960,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3503702"],"award-info":[{"award-number":["2023YFC3503702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National High Level Hospital Clinical Research Funding"},{"name":"Elite Medical Professionals Project of China-Japan Friendship Hospital","award":["ZRJY2023-QM11"],"award-info":[{"award-number":["ZRJY2023-QM11"]}]},{"name":"Elite Medical Professionals Initiative of China-Japan Friendship Hospital","award":["ZRJY2025-QMPY37"],"award-info":[{"award-number":["ZRJY2025-QMPY37"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Coronary heart disease (CHD) is the leading cause of death and disability worldwide. The human microbiota, particularly gut bacteria, plays a role in the development of CHD. However, determining the contribution of gut bacteria translocation to systemic circulation in the progression of atherosclerosis remains challenging. Methods and Results: In this exploratory study, we conducted 16S rRNA\u2013based metagenomic analysis to characterize systemic bacterial profiles in a cohort of 27 patients with CHD (9 with severe coronary artery stenosis and 18 with mild to moderate stenosis). We compared microbial diversity between arterial and venous blood and across different blood fractions. For the first time, we observed higher microbial diversity in plasma than in serum. We also identified differences in microbial richness among arterial whole blood, venous whole blood, arterial plasma, venous plasma, arterial serum, and venous serum, with 15, 22, 43, 10, 4, and 3 genera showing significant differences, respectively. Many of the detected blood taxa belonged to genera typically found in intestinal, oral, or skin microbiota, although their precise source cannot be determined from this study. Conclusions: Our study provides preliminary evidence of distinct bacterial profiles between arterial and venous blood fractions in patients with CHD, as determined by 16S rRNA sequencing. These findings should be interpreted with caution given the small sample size and the absence of a healthy control group, and they warrant confirmation in larger, controlled studies.<\/jats:p>","DOI":"10.3390\/microorganisms14020359","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:46:11Z","timestamp":1770122771000},"page":"359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deciphering the Arterial and Venous Blood Bacterial DNA Profile: Pioneering Insights into Coronary Heart Disease Etiology and Progression"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0856-7223","authenticated-orcid":false,"given":"Mengru","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4082-5931","authenticated-orcid":false,"given":"Lin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianli","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuelin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Integrative Medicine Cardiology, China-Japan Friendship Hospital, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.cpcardiol.2009.10.002","article-title":"Growing epidemic of coronary heart disease in low- and middle-income countries","volume":"35","author":"Gaziano","year":"2010","journal-title":"Curr. Probl. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Raj, P., Thandapilly, S.J., Wigle, J., Zieroth, S., and Netticadan, T. (2021). A Comprehensive Analysis of the Efficacy of Resveratrol in Atherosclerotic Cardiovascular Disease, Myocardial Infarction and Heart Failure. Molecules, 26.","DOI":"10.3390\/molecules26216600"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1253\/circj.CJ-18-0063","article-title":"Inflammatory Mechanisms of Cardiovascular Remodeling","volume":"82","author":"Anzai","year":"2018","journal-title":"Circ. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nrcardio.2014.28","article-title":"The inflammatory response in myocardial injury, repair, and remodelling","volume":"11","author":"Frangogiannis","year":"2014","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Besse, S., Nadaud, S., Balse, E., and Pavoine, C. (2022). Early Protective Role of Inflammation in Cardiac Remodeling and Heart Failure: Focus on TNF\u03b1 and Resident Macrophages. Cells, 11.","DOI":"10.3390\/cells11071249"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Hetta, H.F., Ahmed, R., Ramadan, Y.N., Fathy, H., Khorshid, M., Mabrouk, M.M., and Hashem, M. (2025). Gut virome: New key players in the pathogenesis of inflammatory bowel disease. World J. Methodol., 15.","DOI":"10.5662\/wjm.v15.i2.92592"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1136\/gut.35.8.1090","article-title":"Role of intestinal microflora in chronic inflammation and ulceration of the rat colon","volume":"35","author":"Videla","year":"1994","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Scher, J.U., Sczesnak, A., Longman, R.S., Segata, N., Ubeda, C., Bielski, C., Rostron, T., Cerundolo, V., Pamer, E.G., and Abramson, S.B. (2013). Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis. eLife, 2.","DOI":"10.7554\/eLife.01202"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1097\/01.qco.0000244054.69253.f3","article-title":"Bacteria in the pathogenesis of inflammatory bowel disease","volume":"19","author":"Subramanian","year":"2006","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Harsch, I.A., and Konturek, P.C. (2018). The Role of Gut Microbiota in Obesity and Type 2 and Type 1 Diabetes Mellitus: New Insights into \u201cOld\u201d Diseases. Med. Sci., 6.","DOI":"10.3390\/medsci6020032"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhao, X., Oduro, P.K., Tong, W., Wang, Y., Gao, X., and Wang, Q. (2021). Therapeutic potential of natural products against atherosclerosis: Targeting on gut microbiota. Pharmacol. Res., 163.","DOI":"10.1016\/j.phrs.2020.105362"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhou, X., Li, J., Guo, J., Geng, B., Ji, W., Zhao, Q., Li, J., Liu, X., Liu, J., and Guo, Z. (2018). Gut-dependent microbial translocation induces inflammation and cardiovascular events after ST-elevation myocardial infarction. Microbiome, 6.","DOI":"10.1186\/s40168-018-0441-4"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Chopra, A., Franco-Duarte, R., Rajagopal, A., Choowong, P., Soares, P., Rito, T., Eberhard, J., and Jayasinghe, T.N. (2024). Exploring the presence of oral bacteria in non-oral sites of patients with cardiovascular diseases using whole metagenomic data. Sci. Rep., 14.","DOI":"10.1038\/s41598-023-50891-x"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1128\/CMR.2.4.329","article-title":"Clinical implications of positive blood cultures","volume":"2","author":"Bryan","year":"1989","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nature18850","article-title":"Microbiome-wide association studies link dynamic microbial consortia to disease","volume":"535","author":"Gilbert","year":"2016","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Castillo, D.J., Rifkin, R.F., Cowan, D.A., and Potgieter, M. (2019). The Healthy Human Blood Microbiome: Fact or Fiction?. Front. Cell. Infect. Microbiol., 9.","DOI":"10.3389\/fcimb.2019.00148"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Salter, S.J., Cox, M.J., Turek, E.M., Calus, S.T., Cookson, W.O., Moffatt, M.F., Turner, P., Parkhill, J., Loman, N.J., and Walker, A.W. (2014). Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biol., 12.","DOI":"10.1186\/s12915-014-0087-z"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cheng, H.S., Tan, S.P., Wong, D.M.K., Koo, W.L.Y., Wong, S.H., and Tan, N.S. (2023). The Blood Microbiome and Health: Current Evidence, Controversies, and Challenges. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065633"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.tim.2018.11.003","article-title":"Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations","volume":"27","author":"Eisenhofer","year":"2019","journal-title":"Trends Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1038\/s41564-023-01350-w","article-title":"No evidence for a common blood microbiome based on a population study of 9770 healthy humans","volume":"8","author":"Tan","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1128\/iai.15.2.621-627.1977","article-title":"Novel bacterial structures in human blood: Cultural isolation","volume":"15","author":"Domingue","year":"1977","journal-title":"Infect. Immun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1001\/archinte.162.4.477","article-title":"Identification of Cardiobacterium hominis by broad-range bacterial polymerase chain reaction analysis in a case of culture-negative endocarditis","volume":"162","author":"Nikkari","year":"2002","journal-title":"Arch. Intern. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"788","DOI":"10.18632\/aging.100695","article-title":"Eryptosis as a marker of Parkinson\u2019s disease","volume":"6","author":"Pretorius","year":"2014","journal-title":"Aging"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Xu, J., Wang, X., Ren, X., and Liu, Y. (2019). Changes of intestinal bacterial microbiota in coronary heart disease complicated with nonalcoholic fatty liver disease. BMC Genom., 20.","DOI":"10.1186\/s12864-019-6251-7"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1002\/hep.28829","article-title":"Changes in blood microbiota profiles associated with liver fibrosis in obese patients: A pilot analysis","volume":"64","author":"Lelouvier","year":"2016","journal-title":"Hepatology"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Qian, X., Liu, A., Liang, C., He, L., Xu, Z., and Tang, S. (2022). Analysis of gut microbiota in patients with acute myocardial infarction by 16S rRNA sequencing. Ann. Transl. Med., 10.","DOI":"10.21037\/atm-22-5671"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1017\/S0007114511005137","article-title":"Combination of Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces post-myocardial infarction depression symptoms and restores intestinal permeability in a rat model","volume":"107","author":"Rondeau","year":"2012","journal-title":"Br. J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Bao, D., Xie, X., Cheng, M., Zhang, K., Yue, T., Liu, A., Fang, W., Wei, Y., Zheng, H., and Piao, J.G. (2022). Hydroxy-safflower yellow A composites: An effective strategy to enhance anti-myocardial ischemia by improving intestinal permeability. Int. J. Pharm., 623.","DOI":"10.1016\/j.ijpharm.2022.121918"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6058","DOI":"10.2174\/1381612822666160914182822","article-title":"The Role of Microbiota and Intestinal Permeability in the Pathophysiology of Autoimmune and Neuroimmune Processes with an Emphasis on Inflammatory Bowel Disease Type 1 Diabetes and Chronic Fatigue Syndrome","volume":"22","author":"Morris","year":"2016","journal-title":"Curr. Pharm. Des."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2021). Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes\u20142021. Diabetes Care, 44, S15\u2013S33.","DOI":"10.2337\/dc21-S002"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1042\/CS20100043","article-title":"Gene expression profiling in whole blood of patients with coronary artery disease","volume":"119","author":"Taurino","year":"2010","journal-title":"Clin. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/eurheartj\/ehi718","article-title":"Adherence to guidelines in the clinical care for adults with congenital heart disease: The Euro Heart Survey on adult congenital heart disease","volume":"27","author":"Engelfriet","year":"2006","journal-title":"Eur. Heart J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1007\/s00253-015-7257-y","article-title":"Comparison of the fecal microbiota of dholes high-throughput Illumina sequencing of the V3\u2013V4 region of the 16S rRNA gene","volume":"100","author":"Wu","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Salzberg","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W.S., and Huttenhower, C. (2011). Metagenomic biomarker discovery and explanation. Genome Biol., 12.","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1007\/s00125-011-2329-8","article-title":"Involvement of tissue bacteria in the onset of diabetes in humans: Evidence for a concept","volume":"54","author":"Amar","year":"2011","journal-title":"Diabetologia"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1093\/femsre\/fuv013","article-title":"The dormant blood microbiome in chronic, inflammatory diseases","volume":"39","author":"Potgieter","year":"2015","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1378\/chest.123.3.854","article-title":"Clinical utility of blood cultures drawn from central vein catheters and peripheral venipuncture in critically ill medical patients","volume":"123","author":"Beutz","year":"2003","journal-title":"Chest"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Poupin, N., Tremblay-Franco, M., Amiel, A., Canlet, C., R\u00e9mond, D., Debrauwer, L., Dardevet, D., Thiele, I., Aurich, M.K., and Jourdan, F. (2019). Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-48997-2"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1159\/000447794","article-title":"Plasma MicroRNA-126-5p is Associated with the Complexity and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris","volume":"39","author":"Li","year":"2016","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Stevens, V.L., Hoover, E., Wang, Y., and Zanetti, K.A. (2019). Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review. Metabolites, 9.","DOI":"10.3390\/metabo9080156"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Yu, Z., Kastenm\u00fcller, G., He, Y., Belcredi, P., M\u00f6ller, G., Prehn, C., Mendes, J., Wahl, S., Roemisch-Margl, W., and Ceglarek, U. (2011). Differences between human plasma and serum metabolite profiles. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021230"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.aca.2018.03.009","article-title":"Serum or plasma, what is the difference? Investigations to facilitate the sample material selection decision making process for metabolomics studies and beyond","volume":"1037","author":"Liu","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Youssef, L., Crovetto, F., Simoes, R.V., Miranda, J., Paules, C., Blasco, M., Palomo, M., Garc\u00eda-Calder\u00f3, H., Tura-Ceide, O., and Dantas, A.P. (2022). The Interplay between Pathophysiological Pathways in Early-Onset Severe Preeclampsia Unveiled by Metabolomics. Life, 12.","DOI":"10.3390\/life12010086"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.tibtech.2021.08.006","article-title":"Progress and challenges in mass spectrometry-based analysis of antibody repertoires","volume":"40","author":"Snapkov","year":"2022","journal-title":"Trends Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Amar, J., Lelouvier, B., Servant, F., Lluch, J., Burcelin, R., Bongard, V., and Elbaz, M. (2019). Blood Microbiota Modification After Myocardial Infarction Depends Upon Low-Density Lipoprotein Cholesterol Levels. J. Am. Heart Assoc., 8.","DOI":"10.1161\/JAHA.118.011797"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hetta, H.F., Sirag, N., Elfadil, H., Salama, A., Aljadrawi, S.F., Alfaifi, A.J., Alwabisi, A.N., AbuAlhasan, B.M., Alanazi, L.S., and Aljohani, Y.A. (2025). Artificial Sweeteners: A Double-Edged Sword for Gut Microbiome. Diseases, 13.","DOI":"10.3390\/diseases13040115"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/2\/359\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T13:02:41Z","timestamp":1770123761000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/2\/359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,3]]},"references-count":48,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["microorganisms14020359"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14020359","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,3]]}}}