{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:49:11Z","timestamp":1785898151948,"version":"3.56.0"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hallym University Research Fund"},{"name":"Basic Science Research Program through the National Research Foundation of Korea"},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["NRF-2018M3A9F3020956"],"award-info":[{"award-number":["NRF-2018M3A9F3020956"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["NRF-2019R1I1A3A01060447"],"award-info":[{"award-number":["NRF-2019R1I1A3A01060447"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["NRF-2020R1I1A3073530"],"award-info":[{"award-number":["NRF-2020R1I1A3073530"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"crossref","award":["NRF-2020R1A6A1A03043026"],"award-info":[{"award-number":["NRF-2020R1A6A1A03043026"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Alterations in gut microbiota are closely associated with alcohol-associated liver disease (ALD) progression. We aimed to identify ALD-related bacterial strains with therapeutic or diagnostic potential. Human fecal samples were analyzed to screen candidate microbes, and an ALD mouse model was used to evaluate their effects. We also assessed bacterial DNA levels in blood to explore diagnostic utility. Lactobacillus helveticus and L. lactis treatment improved gut dysbiosis and reduced hepatic inflammation and endotoxemia. In contrast, Veillonella dispar, which is significantly enriched in ALD patients, had no beneficial effects in vivo. Instead, V. dispar abundance in blood distinguished ALD patients from controls with an area under the ROC curve of 0.815. These findings suggest that L. helveticus and L. lactis may be effective probiotics for ALD, while V. dispar may serve as a non-invasive diagnostic biomarker. Targeting microbiota may offer a new approach for ALD prevention and diagnosis.<\/jats:p>","DOI":"10.3390\/microorganisms14020449","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:06:16Z","timestamp":1770905176000},"page":"449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microbial Biomarkers for the Prevention and Diagnosis of Alcoholic Liver Disease"],"prefix":"10.3390","volume":"14","author":[{"given":"Goo Hyun","family":"Kwon","sequence":"first","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjoon","family":"Park","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeong Seop","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4199-7575","authenticated-orcid":false,"given":"Ki Kwang","family":"Oh","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung A","family":"Eom","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0454-0897","authenticated-orcid":false,"given":"Minsoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Su","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hak","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hallym University Chuncheon Sacred Heart Hospital, Chuncheon 24253, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Lim","family":"Ham","sequence":"additional","affiliation":[{"name":"Department of Nursing, Daewon University College, Jecheon 27137, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-9245","authenticated-orcid":false,"given":"Ki Tae","family":"Suk","sequence":"additional","affiliation":[{"name":"Institute for Liver and Digestive Diseases, Hallym University, Chuncheon 24252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"ref_1","unstructured":"GBD 2023 Disease and Injury and Risk Factor Collaborators (2025). 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