{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T10:15:06Z","timestamp":1771409706938,"version":"3.50.1"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)\/Brazil","award":["140016\/2021-0"],"award-info":[{"award-number":["140016\/2021-0"]}]},{"name":"The Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)\/Brazil","award":["313119\/2020-1"],"award-info":[{"award-number":["313119\/2020-1"]}]},{"name":"The Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)\/Brazil","award":["310181\/2021-6"],"award-info":[{"award-number":["310181\/2021-6"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o De Aperfei\u00e7oamento de Pessoal de Nivel Superior (CAPES)\/Brazil","doi-asserted-by":"publisher","award":["88887.999872\/2024-00"],"award-info":[{"award-number":["88887.999872\/2024-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o De Aperfei\u00e7oamento de Pessoal de Nivel Superior (CAPES)\/Brazil","doi-asserted-by":"publisher","award":["PRJNA1166229"],"award-info":[{"award-number":["PRJNA1166229"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro\u2014Brazil","award":["E-26\/200.891\/2021"],"award-info":[{"award-number":["E-26\/200.891\/2021"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro\u2014Brazil","award":["E-26\/204.145\/2022"],"award-info":[{"award-number":["E-26\/204.145\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>(1) Background: We sought to explore the changes in the biomolecular profile and morphology of Pathogenic heat-resistant E. coli isolated from animal-based food. (2) Methods: Six strains underwent heat (60 \u00b0C for 6 min), ultrasound (US; 299 W), UVC (4950 mJ\/cm2), and combined treatments (UVC+US and heat+UVC). Afterwards, biomolecular characterization across four spectral regions was evaluated by Fourier transform infrared (FT-IR) spectroscopy and analyzed by principal component analysis (PCA) for treated and non-treated strains (control group). These regions are fatty acids (3010\u20132800 cm\u22121), proteins and peptides (1700\u20131200 cm\u22121), carbohydrates (1200\u2013900 cm\u22121), and amide A (3280\u20133120 cm\u22121). Additionally, treated and untreated strains were assessed for surface damage using scanning electron microscopy (SEM). (3) Results: Among all the regions studied, the amide A and fatty acids regions exhibited the most significant variations in absorbance for treated strains compared to the control. Treatments such as US, heat, and UVC+US tended to increase Principal Components (PCs) and, consequently, absorbance. On the other hand, UVC and heat+UVC showed the opposite trend in these regions. SEM images showed filamentous cells for strains treated with UVC and UVC+US, indicating that cells continued to replicate under these conditions. These results highlight how thermal and non-thermal treatments influence specific biomolecular and morphological regions of E. coli. The methodologies used provide reliable data for understanding stress responses, which can guide the development of more effective technologies for eliminating multi-resistant pathogens.<\/jats:p>","DOI":"10.3390\/microorganisms14020491","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T09:22:46Z","timestamp":1771406566000},"page":"491","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Use of Thermal and Emerging Non-Thermal Treatments Reveal Biomolecular and Morphological Changes in Pathogenic E. coli"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0189-9017","authenticated-orcid":false,"given":"Maxsueli","family":"Machado","sequence":"first","affiliation":[{"name":"Food Science Program (PPGCAL), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ),  Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Nutrition, Food and Metabolism Program (PPGNAM), Federal University of Mato Grosso (UFMT), Cuiab\u00e1 78060-900, MT, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4886-302X","authenticated-orcid":false,"given":"Jelmir Craveiro","family":"Andrade","sequence":"additional","affiliation":[{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ),  Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2608-4324","authenticated-orcid":false,"given":"Eduardo Eust\u00e1quio de Souza","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Nutrition, Food and Metabolism Program (PPGNAM), Federal University of Mato Grosso (UFMT), Cuiab\u00e1 78060-900, MT, Brazil"},{"name":"Animal Science Program (PPGCA), Federal University of Mato Grosso (UFMT), Cuiab\u00e1 78060-900, MT, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6133-5080","authenticated-orcid":false,"given":"Carlos Adam","family":"Conte-Junior","sequence":"additional","affiliation":[{"name":"Food Science Program (PPGCAL), Chemistry Institute (IQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"},{"name":"Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ),  Rio de Janeiro 21941-598, RJ, Brazil"},{"name":"Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"ref_1","unstructured":"GBD 2019 Antimicrobial Resistance Collaborators (2022). 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