{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T13:21:34Z","timestamp":1771939294865,"version":"3.50.1"},"reference-count":27,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["306857\/2021-9"],"award-info":[{"award-number":["306857\/2021-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordination of Superior Level Staff Improvement (CAPES), Brazil","doi-asserted-by":"publisher","award":["88887.835189\/2023-00"],"award-info":[{"award-number":["88887.835189\/2023-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CNPq\/MCTI\/CT-Sa\u00fade","award":["408898\/2022-4"],"award-info":[{"award-number":["408898\/2022-4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Antimicrobial resistance in bacteria associated with aquaculture, such as Edwardsiella spp., represents an emerging challenge because of their relevance to fish health and their potential impact on animal, environmental, and human health. In this study, we primarily investigated the antimicrobial susceptibility profiles of Edwardsiella anguillarum isolated from farmed Nile tilapia (Oreochromis niloticus) in Brazil. Based on our findings, herein, we propose provisional local epidemiological cut-off values (pECVs) using the normalized resistance interpretation method, with data for Edwardsiella tarda included as an exploratory context. Fifty isolates (31 E. anguillarum and 19 E. tarda) collected between 2017 and 2025 were tested against 28 antibacterial agents using the disk diffusion method. Based on the pECVs, isolates were classified as wild type (WT) or non-WT (NWT), and the multiple antibiotic resistance (MAR) index was calculated. Most E. anguillarum isolates remained susceptible to several classes, although NWT and multidrug-resistant profiles were detected with a MAR index of 0.68, suggesting selective pressure in intensive tilapia farming systems. These findings support the use of local, species-specific pECVs for resistance surveillance in aquaculture, highlighting the importance of continuous antimicrobial resistance monitoring in aquaculture from a One Health perspective.<\/jats:p>","DOI":"10.3390\/microorganisms14030523","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T12:19:23Z","timestamp":1771935563000},"page":"523","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic Antimicrobial Resistance Profiles and Provisional Epidemiological Cut-Off Values of Edwardsiella anguillarum Isolated from Farmed Nile Tilapia (Oreochromis niloticus) in Brazil, with Exploratory Data on Edwardsiella tarda"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1141-659X","authenticated-orcid":false,"given":"Nat\u00e1lia Amoroso","family":"Ferrari","sequence":"first","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitt\u00f3ria Cueva Segura","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela Giovana","family":"Turini","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Faria","family":"de Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella Menegueti","family":"Mainardi","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayza Brand\u00e3o","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1450-2197","authenticated-orcid":false,"given":"Alene Santos","family":"Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Diogo","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maisa Fabiana","family":"Menck-Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1693-7178","authenticated-orcid":false,"given":"Marco","family":"Rozas-Serri","sequence":"additional","affiliation":[{"name":"Pathovet Laboratory, Ribeir\u00e3o Preto 14025-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariene Miyoko","family":"Natori","sequence":"additional","affiliation":[{"name":"Pathovet Laboratory, Ribeir\u00e3o Preto 14025-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Galetti","sequence":"additional","affiliation":[{"name":"Pathovet Laboratory, Ribeir\u00e3o Preto 14025-020, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4868-4459","authenticated-orcid":false,"given":"Ulisses de Padua","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Bacteriology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina 86057-970, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"737643","DOI":"10.1016\/j.aquaculture.2021.737643","article-title":"Prevalence, Antimicrobial Resistance (AMR) 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