{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:39:25Z","timestamp":1791689965449,"version":"4.3.4"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:00:00Z","timestamp":1772841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000738","name":"Department of Veterans Affairs","doi-asserted-by":"crossref","award":["5I01BX001130"],"award-info":[{"award-number":["5I01BX001130"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Endocytic trafficking in Candida albicans is a fundamental cellular process that is crucial for its secretion, filamentation, and virulence-related processes. We have previously demonstrated that loss of the key endocytosis-related C. albicans gene END3 disrupts clathrin-mediated endocytosis, leading to impairments in actin patch formation, filamentation, biofilm formation, cell wall integrity, and extracellular protease secretion. The end3 null mutant also exhibits altered antifungal susceptibility and reduced host-cell damage in an in vitro keratinocyte infection model. To ascertain whether endocytosis is required for virulence in vivo, we assessed virulence of the C. albicans end3 null mutant in a murine model of disseminated candidiasis. After infection via the tail vein, and analysis of host survival over 28 days, the end3 null mutant was markedly hypovirulent compared to corresponding control strains. These results indicate that endocytosis mediated by END3 in C. albicans contributes to pathogenesis in vivo.<\/jats:p>","DOI":"10.3390\/microorganisms14030598","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T11:06:52Z","timestamp":1773054412000},"page":"598","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Endocytosis Mediated by Candida albicans END3 Is Required for Its In Vivo Virulence as an Opportunistic Fungal Pathogen"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0395-9343","authenticated-orcid":false,"given":"Miranda","family":"Yu","sequence":"first","affiliation":[{"name":"Medicine Service, White River Junction VA Medical Center, Hartford, VT 05009, USA"},{"name":"School of Arts & Sciences, Dartmouth College, Hanover, NH 03755, USA"},{"name":"Department of Medicine, University of Nevada Reno, Reno, NV 89250, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Gilmore","sequence":"additional","affiliation":[{"name":"Medicine Service, White River Junction VA Medical Center, Hartford, VT 05009, USA"},{"name":"School of Arts & Sciences, Dartmouth College, Hanover, NH 03755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Dos Santos","sequence":"additional","affiliation":[{"name":"Medicine Service, White River Junction VA Medical Center, Hartford, VT 05009, USA"},{"name":"School of Arts & Sciences, Dartmouth College, Hanover, NH 03755, USA"},{"name":"Department of Medicine, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Eszterhas","sequence":"additional","affiliation":[{"name":"Medicine Service, White River Junction VA Medical Center, Hartford, VT 05009, USA"},{"name":"Department of Medicine, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel A.","family":"Lee","sequence":"additional","affiliation":[{"name":"Medicine Service, White River Junction VA Medical Center, Hartford, VT 05009, USA"},{"name":"Department of Medicine, University of Nevada Reno, Reno, NV 89250, USA"},{"name":"Department of Medicine, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rollenhagen, C., Mamtani, S., Ma, D., Dixit, R., Eszterhas, S., and Lee, S.A. 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