{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:05:34Z","timestamp":1791230734731,"version":"4.1.0"},"reference-count":76,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["ORF-2025-797"],"award-info":[{"award-number":["ORF-2025-797"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Contaminated environmental surfaces (fomites) act as pathogen reservoirs, yet surveillance data in arid megacities like Riyadh, Saudi Arabia\u2014characterized by extreme heat and indoor climate control\u2014remain limited. This study established a city-wide molecular baseline for surface contamination and evaluated meteorological influences. We conducted a stratified, longitudinal study (February 2023\u2013May 2024), collecting 270 swabs from seven zones, including hospitals, airports, ATMs, and community hubs. Samples were pooled (N = 55) and screened using QIAstat-Dx multiplex PCR panels. Nineteen pools (34.5%) tested positive. Viral pathogens (SARS-CoV-2, Adenovirus, Rhinovirus) were detected in 10 pools (18.2%) and non-viral pathogens (bacteria\/parasites) in 13 pools (23.6%), with 7.3% co-detections. Hospitals and airports emerged as primary hubs for respiratory viruses, while Cryptosporidium was the most frequent non-viral pathogen (n = 6), predominating on ATM interfaces. Binary logistic regression indicated that higher ambient temperature was significantly associated with detecting viral rather than non-viral pathogens among positive samples (OR = 1.728, p = 0.032). Despite outdoor aridity, public surfaces in Riyadh harbored diverse pathogens. The link between heat and viral detection suggests indoor microclimates drive persistence during hot seasons, necessitating targeted hygiene measures in high-risk nodes.<\/jats:p>","DOI":"10.3390\/microorganisms14030626","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T17:04:24Z","timestamp":1773162264000},"page":"626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pathogens on High-Touch Surfaces in an Arid Megacity: A Longitudinal Molecular Surveillance Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1302-1682","authenticated-orcid":false,"given":"Mohamad Taisir Ahmad","family":"Ghiba","sequence":"first","affiliation":[{"name":"Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"},{"name":"Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh Ahmed","family":"Eifan","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9257-1385","authenticated-orcid":false,"given":"Abdulkarim Fahad","family":"Alhetheel","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atif","family":"Hanif","sequence":"additional","affiliation":[{"name":"Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/s41579-021-00535-6","article-title":"Transmissibility and Transmission of Respiratory Viruses","volume":"19","author":"Leung","year":"2021","journal-title":"Nat. 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