{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:55:33Z","timestamp":1782953733337,"version":"3.54.5"},"reference-count":76,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["1025120800043-6-4.1.6"],"award-info":[{"award-number":["1025120800043-6-4.1.6"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The isolation of locally adapted rhizobial strains with high symbiotic activity represents an effective strategy for increasing soybean yield under extreme environmental conditions. In this study, seven novel strains were isolated from nodules of soybeans grown in a greenhouse using field soil from the Lower Volga region. Five genomes were assembled into complete circular chromosomes, whereas two strains yielded near-complete chromosomes containing single repeat-mediated junctions. All strains had putative plasmids that were independently validated as circular by long-read mapping and confirmed by the presence of characteristic replication and conjugation-associated genes. Genome sequences of strains were about 11 Mb, and GC contents were 63.1\u201363.3%. Comparative genome analyses demonstrated that all strains had average nucleotide identity values of 95.4% with Bradyrhizobium japonicum USDA 6T and 96.3% with Bradyrhizobium barranii 144S4T, forming a distinct cluster in phylogenetic trees. No significant differences were detected between B. japonicum and B. barranii that would explain the species boundary. Therefore, it is proposed to unite all novel strains into the subspecies Bradyrhizobium japonicum subsp. saratovii subsp. nov., and all other strains of B. japonicum and B. barranii we suggest dividing into four subspecies: Bradyrhizobium japonicum subsp. japonicum subsp. nov., Bradyrhizobium japonicum subsp. barranii comb. nov., Bradyrhizobium japonicum subsp. apii comb. nov., and Bradyrhizobium japonicum subsp. saratovii subsp. nov. The proposed taxonomic framework expands current knowledge of the biodiversity of soybean symbiotic bacteria and contributes to a better understanding of the distribution and the evolution of bacteria Bradyrhizobium spp. in previously unexplored regions.<\/jats:p>","DOI":"10.3390\/microorganisms14030684","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:10:04Z","timestamp":1773825004000},"page":"684","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bacterial Strains from Soybean Nodules in the Lower Volga Region Belong to a New Subspecies Bradyrhizobium japonicum subsp. saratovii subsp. nov."],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3411-5572","authenticated-orcid":false,"given":"Aleksandr S.","family":"Sidorin","sequence":"first","affiliation":[{"name":"Department of Plant Breeding, Selection, and Genetics, Institute of Genetics and Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov 410012, Russia"},{"name":"Russian Research Anti-Plague Institute \u201cMicrobe\u201d, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8031-9641","authenticated-orcid":false,"given":"Gennady L.","family":"Burygin","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Selection, and Genetics, Institute of Genetics and Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov 410012, Russia"},{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Saratov 410049, Russia"},{"name":"Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, Saratov 410012, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7190-4427","authenticated-orcid":false,"given":"Andrey V.","family":"Fedorov","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u201cMicrobe\u201d, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8260-4670","authenticated-orcid":false,"given":"Aleksandr D.","family":"Katyshev","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u201cMicrobe\u201d, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaroslav M.","family":"Krasnov","sequence":"additional","affiliation":[{"name":"Russian Research Anti-Plague Institute \u201cMicrobe\u201d, Saratov 410005, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8327-6763","authenticated-orcid":false,"given":"Oksana V.","family":"Tkachenko","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Selection, and Genetics, Institute of Genetics and Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, Saratov 410012, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"ref_1","first-page":"63","article-title":"Role of biofertilizers in agriculture","volume":"5","author":"Kumar","year":"2017","journal-title":"Pop. Kheti"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6672","DOI":"10.1038\/s41598-024-57008-y","article-title":"Influence of nitrogen fertilization, seed inoculation and the synergistic effect of these treatments on soybean yields under conditions in south-western Poland","volume":"14","author":"Helios","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_3","unstructured":"Sarhadi, W.A., Sarhadi, W.A., Yosofzai, W., Osmani, M.H., Masomi, M., Tungate, K., and Kwon, S. (2023, January 13\u201316). Fertilizers and Inoculants Effects on Soybean Yield and Yield Components. 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