{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:33:29Z","timestamp":1790224409332,"version":"4.1.0"},"reference-count":75,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dokuz Eyl\u00fcl University, Scientific Research Projects Coordination Unit","award":["FBA-2023-3044"],"award-info":[{"award-number":["FBA-2023-3044"]}]},{"award":["FBA-2023-3044"],"award-info":[{"award-number":["FBA-2023-3044"]}],"id":[{"id":"https:\/\/ror.org\/00dbd8b73","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Due to increasing antimicrobial resistance and oxidative stress, the exploration of new therapeutic agents from medicinal plants such as Galium aparine L., has become essential. This study aimed to provide a scientific basis for the ethnomedicinal applications of G. aparine by evaluating its biological activities and biochemical composition. Plant samples were extracted using different solvents and their biological activities were assessed through antimicrobial, antibiofilm, and anti-quorum sensing (AQS) assays against different microorganisms. Antioxidant capacity was determined using four complementary assays representing distinct mechanisms, while the biochemical composition was analyzed via GC\u2013MS. The results revealed that although the extracts exhibited limited antimicrobial activity overall, significant inhibition was observed against Staphylococcus aureus, and Enterococcus strains, with MIC values as low as 30 \u00b5g\/mL. The ethanol extract demonstrated the highest antibiofilm inhibition (60.38%) against Streptococcus mutans, surpassing the positive control Halamid, and showed the strongest AQS inhibition (46.16%). Moreover, a strong antioxidant potential comparable to ascorbic acid was detected in the ABTS assay, with 88.10% inhibition. Overall, these findings indicate that G. aparine extracts possess notable antibiofilm, AQS, and antioxidant properties that support their traditional medicinal uses and suggest their potential as promising sources for the development of new natural therapeutic agents.<\/jats:p>","DOI":"10.3390\/microorganisms14040804","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:51:02Z","timestamp":1775148662000},"page":"804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Bioactivity and Biochemical Composition of Galium aparine L."],"prefix":"10.3390","volume":"14","author":[{"given":"Kerem","family":"Canl\u0131","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, Dokuz Eyl\u00fcl University, Izmir 35390, T\u00fcrkiye"},{"name":"Fauna and Flora Research and Application Center, Dokuz Eyl\u00fcl University, Izmir 35390, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8485-0488","authenticated-orcid":false,"given":"Dilay","family":"Turu","sequence":"additional","affiliation":[{"name":"Department of Biology, Graduate School of Natural and Applied Science, Dokuz Eyl\u00fcl University, Izmir 35390, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6726-5968","authenticated-orcid":false,"given":"Atakan","family":"Benek","sequence":"additional","affiliation":[{"name":"Fauna and Flora Research and Application Center, Dokuz Eyl\u00fcl University, Izmir 35390, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8412-6473","authenticated-orcid":false,"given":"Asl\u0131 Sade","family":"Memi\u015fo\u011flu","sequence":"additional","affiliation":[{"name":"Department of Science Education, Department of Mathematics and Science Teaching, Buca Faculty of Education, Dokuz Eylul University, Izmir 35340, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayd\u0131n","family":"\u00c7\u00f6mez","sequence":"additional","affiliation":[{"name":"General Directorate of Forestry, Ege Forestry Research Institute, \u0130zmir 35530, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arzu","family":"Y\u00fccel","sequence":"additional","affiliation":[{"name":"General Directorate of Forestry, Ege Forestry Research Institute, \u0130zmir 35530, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafize Handan","family":"\u00d6ner","sequence":"additional","affiliation":[{"name":"General Directorate of Forestry, Ege Forestry Research Institute, \u0130zmir 35530, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Burak","family":"Arslan","sequence":"additional","affiliation":[{"name":"General Directorate of Forestry, Ege Forestry 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infections","volume":"292","author":"Levin","year":"2001","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1056\/NEJMra1600266","article-title":"The human intestinal microbiome in health and disease","volume":"375","author":"Lynch","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1056\/NEJMra1208623","article-title":"Severe sepsis and septic shock","volume":"369","author":"Angus","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"ref_4","unstructured":"World Health Organization (2019). Global Antimicrobial Resistance Surveillance System (GLASS) Report: Early Implementation 2017\u20132018, World Health Organization."},{"key":"ref_5","unstructured":"(2025, March 10). Centers for Disease Control and Prevention, Available online: https:\/\/www.cdc.gov\/antimicrobial-resistance\/media\/pdfs\/2019-ar-threats-report-508.pdf."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/cid\/ciz089","article-title":"Infectious Diseases Society of America. The Infectious Diseases Society of America\u2019s 10\u00d7\u201920 initiative (10 new systemic antibacterial agents US Food and Drug Administration approved by 2020): Is 20\u00d7\u201920 a possibility?","volume":"69","author":"Talbot","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s11908-019-0690-9","article-title":"Recognizing and overcoming resistance to new beta-lactam\/beta-lactamase inhibitor combinations","volume":"21","author":"Ho","year":"2019","journal-title":"Curr. Infect. Dis. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mulani, M.S., Kamble, E.E., Kumkar, S.N., Tawre, M.S., and Pardesi, K.R. (2019). Emerging Strategies to Combat ESKAPE Pathogens in the Era of Antimicrobial Resistance: A Review. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00539"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00181-19","DOI":"10.1128\/CMR.00181-19","article-title":"Antimicrobial Resistance in ESKAPE Pathogens","volume":"33","author":"Forde","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8416763","DOI":"10.1155\/2017\/8416763","article-title":"Oxidative stress: Harms and benefits for human health","volume":"2017","author":"Pizzino","year":"2017","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_11","first-page":"1","article-title":"Oxidative stress and antioxidants in health and disease","volume":"47","author":"Otlu","year":"2023","journal-title":"Am. J. Lab. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1007\/s13197-014-1396-5","article-title":"Functional components and medicinal properties of food: A review","volume":"52","author":"Abuajah","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4757","DOI":"10.1007\/s13197-019-03952-x","article-title":"The importance of antioxidants and place in today\u2019s scientific and technological studies","volume":"56","author":"Zehiroglu","year":"2019","journal-title":"J. Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2147\/CIA.S158513","article-title":"Oxidative stress, aging, and diseases","volume":"13","author":"Liguori","year":"2018","journal-title":"Clin. Interv. Aging"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.lfs.2018.12.029","article-title":"Crosstalk between mitochondrial dysfunction, oxidative stress, and age related neurodegenerative disease: Etiologies and therapeutic strategies","volume":"218","author":"Elfawy","year":"2019","journal-title":"J. Life Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/S0895-3988(15)30111-2","article-title":"Cancer therapy with phytochemicals: Present and future perspectives","volume":"28","author":"Tuorkey","year":"2015","journal-title":"Biomed. Environ. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.jep.2012.01.056","article-title":"UPLC\u2013TOF-MS analysis of Galium spurium towards its neuroprotective and anticonvulsant activities","volume":"141","author":"Orhan","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1046\/j.1365-2745.1999.00381.x","article-title":"Galium aparine L.","volume":"87","author":"Taylor","year":"1999","journal-title":"J. Ecol."},{"key":"ref_19","unstructured":"Tobyn, G., Denham, A., and Whitelegg, M. (2016). The Western Herbal Tradition: 2000 Years of Medicinal Plant Knowledge, Singing Dragon. [1st ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Laanet, P.R., Saar-Reismaa, P., J\u00f5ul, P., Bragina, O., and Vaher, M. (2023). Phytochemical screening and antioxidant activity of selected Estonian Galium species. Molecules, 28.","DOI":"10.3390\/molecules28062867"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Petkova, M.K., Grozeva, N.H., Tzanova, M.T., and Todorova, M.H. (2025). A review of phytochemical and pharmacological studies on Galium verum L., Rubiaceae. Molecules, 30.","DOI":"10.3390\/molecules30081856"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhao, A., Sun, J., and Liu, Y. (2023). Understanding bacterial biofilms: From definition to treatment strategies. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1137947"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1128\/CMR.12.4.564","article-title":"Plant products as antimicrobial agents","volume":"12","author":"Cowan","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_24","first-page":"3201458","article-title":"In vitro antimicrobial activity screening of ethanol extract of Lavandula stoechas and investigation of its biochemical composition","volume":"2019","author":"Yetgin","year":"2019","journal-title":"Adv. Pharmacol. Pharm. Sci."},{"key":"ref_25","first-page":"539","article-title":"Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme","volume":"8","author":"Altuner","year":"2014","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/jac\/48.suppl_1.5","article-title":"Determination of minimum inhibitory concentrations","volume":"48","author":"Andrews","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","unstructured":"(2024). CLSI Standard M07; Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, Clinical and Laboratory Standards Institute. [12th ed.]."},{"key":"ref_28","unstructured":"(2026). CLSI Standard M100; Performance Standards for Antimicrobial Susceptibility Testing, Clinical and Laboratory Standards Institute. [36th ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kowalska-Krochmal, B., and Dudek-Wicher, R. (2021). The minimum inhibitory concentration of antibiotics: Methods, interpretation, clinical relevance. Pathogens, 10.","DOI":"10.3390\/pathogens10020165"},{"key":"ref_30","first-page":"126","article-title":"Antimicrobial activity of Isothecium alopecuroides and potential effect of some climate elements on the activity of this bryophyte sample","volume":"18","author":"Altuner","year":"2018","journal-title":"Kastamonu Un\u0131v. J. For."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/j.1472-765X.2009.02747.x","article-title":"The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes","volume":"50","author":"Sandasi","year":"2010","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_32","first-page":"69","article-title":"Anti-biofilm and antimicrobial activities of five edible and medicinal macrofungi samples on some biofilm producing multi drug resistant Enterococcus strains","volume":"8","author":"Karaca","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"ref_33","unstructured":"Ayd\u0131n, F. (2001). Investigation of the susceptibilities of Pseudomonas aeruginosa strains isolated from various clinical samples to various antimicrobials using different methods. [Master\u2019s Thesis, Ankara University]."},{"key":"ref_34","unstructured":"Wilson, S.G., and Miles, A.A. (1964). Principles of Bacteriology and Immunity, Edward Arnold Ltd.. [1st ed.]."},{"key":"ref_35","first-page":"75","article-title":"Baz\u0131 u\u00e7ucu ya\u011flar\u0131n P. aeruginosa PAO1 vir\u00fclans\u0131nda etkinli\u011fi","volume":"4","year":"2021","journal-title":"J. Med. Fac. Clin."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Mishra, R., Kushveer, J.S., Khan, M.I.K., Pagal, S., Meena, C.K., Murali, A., and Venkateswara Sarma, V. (2020). 2,4-Ditert-butylphenol isolated from an endophytic fungus, Daldinia eschscholtzii, reduces virulence and quorum sensing in Pseudomonas aeruginosa. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01668"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/ptr.687","article-title":"Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method","volume":"15","author":"Mensor","year":"2001","journal-title":"Phytother. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.3390\/12071496","article-title":"Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay","volume":"12","author":"Apak","year":"2007","journal-title":"Molecules"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3109\/10715769709097799","article-title":"Factors influencing the antioxidant activity determined by the ABTS\u2022+ radical cation assay","volume":"26","author":"Miller","year":"1997","journal-title":"Free Radic. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Simsek, O., Canli, K., Benek, A., Turu, D., and Altuner, E.M. (2023). Biochemical, antioxidant properties and antimicrobial activity of epiphytic leafy liverwort Frullania dilatata (L.) Dumort. Plants, 12.","DOI":"10.3390\/plants12091877"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"434","DOI":"10.15414\/jmbfs.2015.4.5.434-436","article-title":"Antimicrobial evaluation of sesquiterpene alpha-curcumene and its synergism with imipenem","volume":"4","author":"Pozzatti","year":"2015","journal-title":"J. Microbiol. Biotechnol. Food Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3109\/13880209.2014.923003","article-title":"Evaluation of p-cymene, a natural antioxidant","volume":"53","year":"2015","journal-title":"Pharm. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Marchese, A., Arciola, C.R., Barbieri, R., Silva, A.S., Nabavi, S.F., Tsetegho Sokeng, A.J., and Nabavi, S.M. (2017). Update on monoterpenes as antimicrobial agents: A particular focus on p-cymene. Materials, 10.","DOI":"10.3390\/ma10080947"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"15587","DOI":"10.3390\/molecules181215587","article-title":"Antimicrobial and antioxidant activities and effect of 1-hexadecene addition on palmarumycin C2 and C3 yields in liquid culture of endophytic fungus Berkleasmium sp. Dzf12","volume":"18","author":"Mou","year":"2013","journal-title":"Molecules"},{"key":"ref_47","first-page":"455","article-title":"Semisynthesis of myristic acid derivatives and their biological activities: A critical insight","volume":"10","author":"Avid","year":"2020","journal-title":"J. Biol. Active Prod. Nat."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gonzalez-Rivera, M.L., Barragan-Galvez, J.C., Gasca-Mart\u00ednez, D., Hidalgo-Figueroa, S., Isiordia-Espinoza, M., and Alonso-Castro, A.J. (2023). In vivo neuropharmacological effects of neophytadiene. Molecules, 28.","DOI":"10.3390\/molecules28083457"},{"key":"ref_49","first-page":"2587","article-title":"Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga","volume":"22","author":"Harada","year":"2002","journal-title":"Anticancer. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.fsi.2019.10.055","article-title":"Antiviral activity of palmitic acid via autophagic flux inhibition in zebrafish (Danio rerio)","volume":"95","author":"Pereiro","year":"2019","journal-title":"Fish. Shellfish. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.taap.2012.07.020","article-title":"Anti-inflammatory activity of methyl palmitate and ethyl palmitate in different experimental rat models","volume":"264","author":"Saeed","year":"2012","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Bu, C.Y., Duan, D.D., Wang, Y.N., Ma, L.Q., Liu, Y.B., and Shi, G.L. (2012). Acaricidal activity of ethyl palmitate against Tetranychus cinnabarinus. Information Technology and Agricultural Engineering, Springer.","DOI":"10.1007\/978-3-642-27537-1_84"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1080\/14786419.2013.869692","article-title":"Further in vitro evaluation of antiradical and antimicrobial activities of phytol","volume":"28","author":"Pejin","year":"2014","journal-title":"Nat. Prod. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.bpc.2008.07.001","article-title":"Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH techniques","volume":"137","author":"Fagali","year":"2008","journal-title":"Biophys. Chem."},{"key":"ref_55","first-page":"333","article-title":"In vitro and in vivo antimalarial activity of linolenic and linoleic acids and their methyl esters","volume":"4","author":"Melariri","year":"2012","journal-title":"Adv. Stud. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Jung, S.W., Thamphiwatana, S., Zhang, L., and Obonyo, M. (2015). Mechanism of antibacterial activity of liposomal linolenic acid against Helicobacter pylori. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0116519"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1055\/s-2002-36346","article-title":"Antibacterial activity of a stearic acid derivative from Stemodia foliosa","volume":"68","author":"Nascimento","year":"2002","journal-title":"Planta Med."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gazali, M., Jolanda, O., Husni, A., Majid, F.A.A., and Syafitri, R. (2023). In vitro alpha-amylase and alpha-glucosidase inhibitory activity of green seaweed Halimeda tuna extract from the coast of Lhok Bubon, Aceh. Plants, 12.","DOI":"10.3390\/plants12020393"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"42","DOI":"10.5530\/pc.2016.1.8","article-title":"Antimicrobial activity of the genus Galium L.","volume":"6","author":"Ilyina","year":"2016","journal-title":"Pharmacogn. Commun."},{"key":"ref_60","first-page":"7","article-title":"Phytochemical research of Galium aparine L. lipophilic complex and study of its antibacterial activity","volume":"3","author":"Goryacha","year":"2014","journal-title":"J. Pharm. Innov."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"14","DOI":"10.5582\/bst.2016.01020","article-title":"Nosocomial infection and its molecular mechanisms of antibiotic resistance","volume":"10","author":"Xia","year":"2016","journal-title":"Biosci. Trends"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e03273-24","DOI":"10.1128\/spectrum.03273-24","article-title":"Anti-bacterial activity of dermcidin in human platelets: Suppression of methicillin-resistant Staphylococcus aureus growth","volume":"13","author":"Liu","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"ref_63","unstructured":"World Health Organization (2025, September 20). Antimicrobial Resistance. Available online: https:\/\/www.who.int\/docs\/default-source\/antimicrobial-resistance\/amr-factsheet.pdf."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Beirami, A.D., Akhtari, N., Noroozi, R., Hatamabadi, D., Hasan, S.M.F., Ayatollahi, S.A., and Kobarfard, F. (2024). Bringing back Galium aparine L. from forgotten corners of traditional wound treatment procedures: An antimicrobial, antioxidant, and in-vitro wound healing assay along with HPTLC fingerprinting study. BMC Complement. Med. Ther., 24.","DOI":"10.1186\/s12906-024-04355-y"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"107277","DOI":"10.1016\/j.micpath.2025.107277","article-title":"Biofilm battle: New transformative tactics to tackle the bacterial biofilm infections","volume":"199","author":"Upadhyay","year":"2025","journal-title":"Microb. Pathog."},{"key":"ref_66","first-page":"104085","article-title":"Quenching and quorum sensing in bacterial bio-films","volume":"175","author":"Kameswaran","year":"2024","journal-title":"Res. J. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2475067","DOI":"10.1155\/2016\/2475067","article-title":"Mechanisms of antimicrobial resistance in ESKAPE pathogens","volume":"2016","author":"Santajit","year":"2016","journal-title":"Biomed. Res. Int."},{"key":"ref_68","first-page":"251","article-title":"Antioxidant activity of Galium aparine L. from Punjab, Pakistan","volume":"44","author":"Neelam","year":"2012","journal-title":"Pak. J. Bot."},{"key":"ref_69","first-page":"17","article-title":"The effect of extraction methods on antioxidant and enzyme inhibitory activities and phytochemical components of Galium aparine L. Trak","volume":"22","year":"2021","journal-title":"Univ. J. Nat. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"85","DOI":"10.15835\/hpm.v26i1-2.13236","article-title":"Galium species-polyphenolic content and their antioxidant potential","volume":"26","author":"Hanganu","year":"2018","journal-title":"Hop Med. Plants"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1134\/S0003683824605158","article-title":"Mechanisms of the antimicrobial action of fatty acids: A review","volume":"60","author":"Obukhova","year":"2024","journal-title":"Appl. Biochem. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"6217","DOI":"10.1002\/jsfa.14334","article-title":"Unraveling the genetic diversity, seed oil fatty acid constituents and antioxidant activity of different okra (Abelmoschus esculentus (L.) Moench) populations","volume":"105","author":"Talebi","year":"2025","journal-title":"J. Sci. Food Agric."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"114258","DOI":"10.1016\/j.foodres.2024.114258","article-title":"Structural identification and antioxidant activity of trans-9, trans-11, cis-15-conjugated linolenic acid converted by probiotics","volume":"184","author":"Wu","year":"2024","journal-title":"Int. Food Res. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e202400117","DOI":"10.1002\/cbdv.202400117","article-title":"Analyzing the antioxidant activity and fatty acid composition of monofloral mullein (Verbascum sp.) pollen oil obtained via various extraction techniques","volume":"21","author":"Dursun","year":"2024","journal-title":"Chem. Biodivers."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"25","DOI":"10.26672\/anatolianbryology.1434173","article-title":"Determination of biochemical content and antioxidant activity of Calliergonella cuspidata (Hedw.) 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