{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T23:38:58Z","timestamp":1777765138180,"version":"3.51.4"},"reference-count":28,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Science and Technology Commissioner Program","award":["2024S2069"],"award-info":[{"award-number":["2024S2069"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Avian reovirus (ARV) causes viral arthritis and leads to considerable economic losses in the poultry industry. In this study, six ARV strains of distinct genotypes (FJNP01\u2013FJNP06) were isolated from commercial broiler farms. Through gene sequencing and pathogenicity assessment, we analyzed the genetic evolution and pathogenic characteristics of the \u03c3C, P10, \u03c3B, \u03bcB, and \u03bbC genes. Pathogenicity tests revealed that inoculation with FJNP01\u2013FJNP06 by footpad or oral gavage induced symptoms in specific-pathogen-free (SPF) chickens, including mortality and growth retardation. Among the isolates, FJNP04 (genotype IV) showed the highest pathogenicity, causing increased mortality, weight loss, and severe lesions in the footpads and bursa of Fabricius, followed by FJNP05 and FJNP02. The pathogenicity of FJNP06 varied by inoculation route, with enhanced pathogenicity observed following oral gavage. In contrast, FJNP01 and FJNP03 demonstrated relatively low pathogenicity. Identity analysis indicated that \u03c3C and P10 were highly variable, \u03c3B was relatively conserved, while \u03bcB and \u03bbC displayed considerable divergence. Phylogenetic analysis placed FJNP01\u2013FJNP06 into genotypes I to VI, respectively, forming six distinct branches on the \u03c3C and P10 phylogenetic trees, yet clustering more closely on the \u03c3B, \u03bcB, and \u03bbC trees. The pathogenicity of different genotypes of ARV varies, among which FJNP04 (genotype IV) exhibits the strongest pathogenicity. Genetic sequence analysis revealed that \u03c3C and P10 are highly variable, \u03c3B is relatively conserved, while \u03bcB and \u03bbC display a wide range of variation. This study provides insights into the genetic variation and pathogenic characteristics of ARV and serves as a reference for future research.<\/jats:p>","DOI":"10.3390\/microorganisms14040942","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T12:00:32Z","timestamp":1776859232000},"page":"942","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and Pathogenicity Diversity of Six Avian Reovirus Strains with Different Genotypes"],"prefix":"10.3390","volume":"14","author":[{"given":"Xuemei","family":"Lu","sequence":"first","affiliation":[{"name":"College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guowei","family":"He","sequence":"additional","affiliation":[{"name":"College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyang","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8298-6576","authenticated-orcid":false,"given":"Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou 350002, China"},{"name":"Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9802-7548","authenticated-orcid":false,"given":"Yijian","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou 350002, China"},{"name":"Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.1016\/j.psj.2019.11.067","article-title":"Sequencing and phylogenetic analysis of partial S1 genes of avian orthoreovirus isolates in Shandong province during 2015\u20132017","volume":"99","author":"Chen","year":"2020","journal-title":"Poult. 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