{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:08:46Z","timestamp":1792789726789,"version":"4.5.0"},"reference-count":45,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation","doi-asserted-by":"publisher","award":["32560051"],"award-info":[{"award-number":["32560051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32560051"],"award-info":[{"award-number":["32560051"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Xinjiang Leading Talent Introduction Program Science and Technology Key Innovation Platform (Base) Talent Recruitment Project","award":["XJRC-2025-BTKJ-YJ-CXPT-228"],"award-info":[{"award-number":["XJRC-2025-BTKJ-YJ-CXPT-228"]}]},{"DOI":"10.13039\/501100008559","name":"Tarim University President\u2019s Fund","doi-asserted-by":"publisher","award":["TDZKBS202637"],"award-info":[{"award-number":["TDZKBS202637"]}],"id":[{"id":"10.13039\/501100008559","id-type":"DOI","asserted-by":"publisher"}]},{"award":["TDZKBS202637"],"award-info":[{"award-number":["TDZKBS202637"]}],"id":[{"id":"https:\/\/ror.org\/05202v862","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Acinetobacter baumannii, a prominent opportunistic pathogen in healthcare settings, causes severe infections and poses significant challenges for clinical treatment. This study investigates the synergistic effects of \u03b1-pinene combined with meropenem (MEM) on A. baumannii biofilm formation and lung injury in mice, aiming to develop new strategies to combat persistent infections and antibiotic resistance. \u03b1-pinene combined with MEM exhibited strong synergistic antibacterial activity against carbapenem-resistant A. baumannii (CRAB 5E9). The combination significantly inhibited biofilm formation, extracellular polymer production, surface motility, and quorum sensing. The expression of key genes such as ompA, bfmR, bap, csuAB, abaI, and abaR was reduced by up to 61%. In vivo, the treatment alleviated weight loss, decreased the bacterial load in lung tissue, and reduced lung inflammation. Furthermore, it significantly suppressed proteins involved in the inflammatory response and the MAPK pathway, including TLR4, NF-\u03baB, NLRP3, TRAF6, ERK2, p38 MAPK, JNK, and TNF-\u03b1. The combination of \u03b1-pinene and MEM synergistically inhibits A. baumannii biofilm formation and alleviates the inflammatory response in a mouse model, offering a potential therapeutic approach for combating A. baumannii infections.<\/jats:p>","DOI":"10.3390\/microorganisms14050968","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:01:18Z","timestamp":1777474878000},"page":"968","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synergistic Inhibition of Acinetobacter baumannii Biofilm Formation and Reduction of Lung Inflammation In Vivo by Combination of \u03b1-Pinene and Meropenem"],"prefix":"10.3390","volume":"14","author":[{"given":"Shengqiang","family":"Yang","sequence":"first","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise 533000, China"},{"name":"Bio-Based Active Substances Database Construction and Application Technology Innovation Center, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqi","family":"Mu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise 533000, China"},{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science and Technology, Tarim University, Alar 843300, China"},{"name":"Bio-Based Active Substances Database Construction and Application Technology Innovation Center, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4172-174X","authenticated-orcid":false,"given":"Hong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance, Youjiang Medical University for Nationalities, Baise 533000, China"},{"name":"Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, College of Life Science and Technology, Tarim University, Alar 843300, China"},{"name":"Bio-Based Active Substances Database Construction and Application Technology Innovation Center, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"eadp6604","DOI":"10.1126\/sciadv.adp6604","article-title":"Fluoroamphiphilic polymers exterminate multidrug-resistant Gram-negative ESKAPE pathogens while attenuating drug resistance","volume":"10","author":"Zhou","year":"2024","journal-title":"Sci. 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