{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:36:40Z","timestamp":1785404200314,"version":"3.56.0"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shandong Natural Science Foundation of Youth project","award":["ZR2022QC171"],"award-info":[{"award-number":["ZR2022QC171"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32001659"],"award-info":[{"award-number":["32001659"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32360598"],"award-info":[{"award-number":["32360598"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32001659"],"award-info":[{"award-number":["32001659"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32360598"],"award-info":[{"award-number":["32360598"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018627","name":"Science and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province","doi-asserted-by":"crossref","award":["2022KJD088"],"award-info":[{"award-number":["2022KJD088"]}],"id":[{"id":"10.13039\/501100018627","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Oenococcus oeni (O. oeni) can initiate and complete the malolactic fermentation (MLF) process, which significantly improves wine quality. However, stress factors commonly encountered in wine, such as acid stress and ethanol stress, can hinder this process. Overexpression of certain key functional genes using genetic recombination technology can enhance the stress tolerance of O. oeni. In this study, the large-conductance mechanosensitive channel (mscl) gene was overexpressed in O. oeni SD-2a using genetic recombination technology. The results showed that overexpression of this gene enhanced the growth rate of O. oeni under 10% ethanol stress conditions. Physiological index measurements indicated that overexpression of this gene enhanced the control of cell membrane permeability in the recombinant strain at different time points under ethanol stress and altered cell membrane fluidity at these time points. Proteomic analysis after 12 h of treatment under 10% ethanol stress revealed that mscl overexpression significantly altered the protein expression pattern of O. oeni. The most significantly affected proteins included some cell membrane transporters (for sugars, lipids, amino acids, and nucleotides) and proteins involved in cell wall synthesis. These results suggest that mscl overexpression enhances the ethanol stress tolerance of O. oeni by altering its cell membrane properties and affecting the expression levels of proteins related to cell membrane transport and cell wall synthesis. This study provides a theoretical reference for obtaining O. oeni recombinant strains with enhanced stress tolerance through genetic recombination technology.<\/jats:p>","DOI":"10.3390\/microorganisms14050973","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:01:18Z","timestamp":1777474878000},"page":"973","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Overexpression of the Large-Conductance Mechanosensitive Channel Gene in Oenococcus oeni Enhances Its Ethanol Stress Tolerance"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-165X","authenticated-orcid":false,"given":"Longxiang","family":"Liu","sequence":"first","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lemeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Peng","sequence":"additional","affiliation":[{"name":"Gansu Province Wine Industry Technology Research and Development Center, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Enology, Northwest A&F University, Xinong Road 22, Yangling 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Fang","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyu","family":"Song","sequence":"additional","affiliation":[{"name":"Binzhou Key Laboratory of Edible Fungi Breeding and High Value Utilization (Preparation), College of Biological and Pharmaceutical Engineering, Shandong University of Aeronautics, Binzhou 256600, China"},{"name":"Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou 256600, 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