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N6-methyladenosine (m6A) in RNA is known to regulate various physiological and disease processes, and is also involved in the regulation of pathogenic and developmental processes in many pathogens. However, the specific impact of m6A modification on the intracellular growth of C. trachomatis remains poorly understood. In this study, our analysis of the m6A methylation profiles of host cell mRNAs following C. trachomatis infection revealed significant alterations in the distribution of m6A modifications, methylation motifs, and m6A-modified host target genes. We further demonstrate that chlamydial intracellular reproduction is mediated by the host methyltransferase-like (METTL) enzyme METTL14. Silencing METTL14 significantly reduced the reproduction efficiency of C. trachomatis. Mechanistically, C. trachomatis activates the Mitogen-Activated Protein Kinase (MAPK) and Phosphatidylinositol 3-kinase\/Protein Kinase B (PI3K\/AKT) signaling pathways through METTL14, thereby inhibiting host cell apoptosis and promoting intracellular bacterial reproduction. Collectively, these findings identify METTL14 as a key host factor for chlamydial intracellular reproduction, providing new mechanistic insights and potential targets for therapeutic intervention.<\/jats:p>","DOI":"10.3390\/microorganisms14051025","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T14:35:53Z","timestamp":1777559753000},"page":"1025","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["METTL14-Mediated Inhibition of Apoptosis via the MAPK and PI3K\/AKT Pathways Promotes Chlamydia trachomatis Reproduction"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7523-355X","authenticated-orcid":false,"given":"Wenbo","family":"Lei","sequence":"first","affiliation":[{"name":"Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yewei","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yating","family":"Wen","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrong","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8342-3997","authenticated-orcid":false,"given":"Zhongyu","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Department of Clinical Laboratory Medicine, Institution of Microbiology and Infectious Diseases, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.ijbiomac.2016.12.045","article-title":"Genome analysis of Chlamydia trachomatis for functional characterization of hypothetical proteins to discover novel drug targets","volume":"96","author":"Rahman","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.chom.2018.04.002","article-title":"Combined Human Genome-wide RNAi and Metabolite Analyses Identify IMPDH as a Host-Directed Target against Chlamydia Infection","volume":"23","author":"Rother","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"97","DOI":"10.4103\/ijo.IJO_870_16","article-title":"Chlamydial eye infections: Current perspectives","volume":"65","author":"Satpathy","year":"2017","journal-title":"Indian J. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1128\/CMR.00035-15","article-title":"Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women","volume":"28","author":"Menon","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_5","first-page":"285","article-title":"The prevalence of Chlamydia trachomatis in patients who remained symptomatic after completion of sexually transmitted infection treatment","volume":"11","author":"Afrakhteh","year":"2013","journal-title":"Iran. J. Reprod. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.chom.2016.04.002","article-title":"Posttranscriptional m6A Editing of HIV-1 mRNAs Enhances Viral Gene Expression","volume":"19","author":"Kennedy","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chen, S., Kumar, S., Espada, C.E., Tirumuru, N., Cahill, M.P., Hu, L., He, C., and Wu, L. (2021). N6-methyladenosine modification of HIV-1 RNA suppresses type-I interferon induction in differentiated monocytic cells and primary macrophages. PLoS Pathog., 17.","DOI":"10.1371\/journal.ppat.1009421"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1038\/s41564-024-01638-5","article-title":"Single-molecule epitranscriptomic analysis of full-length HIV-1 RNAs reveals functional roles of site-specific m6As","volume":"9","author":"Baek","year":"2024","journal-title":"Nat. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109091","DOI":"10.1016\/j.celrep.2021.109091","article-title":"METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection","volume":"35","author":"Li","year":"2021","journal-title":"Cell Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1038\/s41392-024-01956-4","article-title":"Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2","volume":"9","author":"Zhu","year":"2024","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1158\/1541-7786.MCR-23-0720","article-title":"Hepatitis B Virus-Mediated m6A Demethylation Increases Hepatocellular Carcinoma Stemness and Immune Escape","volume":"22","author":"Meng","year":"2024","journal-title":"Mol. Cancer Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"114756","DOI":"10.1016\/j.celrep.2024.114756","article-title":"HSV-1-induced N6-methyladenosine reprogramming via ICP0-mediated suppression of METTL14 potentiates oncolytic activity in glioma","volume":"43","author":"Chen","year":"2024","journal-title":"Cell Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e2022024118","DOI":"10.1073\/pnas.2022024118","article-title":"N6-methyladenosine modification of HCV RNA genome regulates cap-independent IRES-mediated translation via YTHDC2 recognition","volume":"118","author":"Kim","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/s42003-022-03566-8","article-title":"N6-methyladenosine modification of the Aedes aegypti transcriptome and its alteration upon dengue virus infection in Aag2 cell line","volume":"5","author":"Dai","year":"2022","journal-title":"Commun. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5599","DOI":"10.1038\/s44319-024-00310-7","article-title":"Severe fever with thrombocytopenia syndrome virus induces lactylation of m6A reader protein YTHDF1 to facilitate viral replication","volume":"25","author":"Liu","year":"2024","journal-title":"EMBO Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/s41421-024-00653-4","article-title":"Mycobacterium tuberculosis inhibits METTL14-mediated m6A methylation of Nox2 mRNA and suppresses anti-TB immunity","volume":"10","author":"Ma","year":"2024","journal-title":"Cell Discov."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nchembio.1432","article-title":"A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation","volume":"10","author":"Liu","year":"2014","journal-title":"Nat. Chem. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/cr.2014.3","article-title":"Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase","volume":"24","author":"Ping","year":"2014","journal-title":"Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/nchembio.687","article-title":"N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO","volume":"7","author":"Jia","year":"2011","journal-title":"Nat. Chem. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.molcel.2012.10.015","article-title":"ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility","volume":"49","author":"Zheng","year":"2013","journal-title":"Mol. Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/s12943-022-01549-1","article-title":"N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma","volume":"21","author":"Chen","year":"2022","journal-title":"Mol. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/s12943-019-1109-9","article-title":"Functions of N6-methyladenosine and its role in cancer","volume":"18","author":"He","year":"2019","journal-title":"Mol. Cancer"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhu, W., Zhang, H., and Wang, S. (2022). Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis via Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which is Regulated by METTL3 and YTHDF3. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.861734"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1158\/0008-5472.CAN-23-2081","article-title":"RUVBL1\/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis","volume":"84","author":"Chen","year":"2024","journal-title":"Cancer Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wen, Y., Chen, H., Luo, F., Zhao, L., Shu, M., Su, S., Zhao, Y., Huang, Q., and Li, Z. (2020). Chlamydia trachomatis Plasmid Protein pORF5 Up-Regulates ZFAS1 to Promote Host Cell Survival via MAPK\/p38 Pathway. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.593295"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Subbarayal, P., Karunakaran, K., Winkler, A.C., Rother, M., Gonzalez, E., Meyer, T.F., and Rudel, T. (2015). EphrinA2 receptor (EphA2) is an invasion and intracellular signaling receptor for Chlamydia trachomatis. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004846"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"ftx111","DOI":"10.1093\/femspd\/ftx111","article-title":"Chlamydia trachomatis plasmid-encoded protein pORF5 protects mitochondrial function by inducing mitophagy and increasing HMGB1 expression","volume":"75","author":"Lei","year":"2017","journal-title":"Pathog. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.molcel.2019.11.007","article-title":"Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection","volume":"77","author":"Gokhale","year":"2020","journal-title":"Mol. Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.cell.2012.05.003","article-title":"Comprehensive analysis of mRNA methylation reveals enrichment in 3\u2032 UTRs and near stop codons","volume":"149","author":"Meyer","year":"2012","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.celrep.2014.05.048","article-title":"Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5\u2032 sites","volume":"8","author":"Schwartz","year":"2014","journal-title":"Cell Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1007\/s10495-013-0865-z","article-title":"Chlamydia inhibit host cell apoptosis by inducing Bag-1 via the MAPK\/ERK survival pathway","volume":"18","author":"Kun","year":"2013","journal-title":"Apoptosis"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00284-011-9985-2","article-title":"Chlamydial antiapoptotic activity involves activation of the Raf\/MEK\/ERK survival pathway","volume":"63","author":"Du","year":"2011","journal-title":"Curr. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1038\/s41418-022-00995-0","article-title":"Chlamydia trachomatis inhibits apoptosis in infected cells by targeting the pro-apoptotic proteins Bax and Bak","volume":"29","author":"Gentle","year":"2022","journal-title":"Cell Death Differ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e2100606","DOI":"10.1002\/advs.202100606","article-title":"Methyltransferase-Like Protein 14 Attenuates Mitochondrial Antiviral Signaling Protein Expression to Negatively Regulate Antiviral Immunity via N6-methyladenosine Modification","volume":"8","author":"Qin","year":"2021","journal-title":"Adv. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chen, J., Song, H.X., Hu, J.H., Bai, J.S., Li, X.H., Sun, R.C., Zhao, B.Q., Li, M.Z., and Zhou, B. (2024). Classical swine fever virus non-structural protein 5B hijacks host METTL14-mediated m6A modification to counteract host antiviral immune response. PLoS Pathog., 20.","DOI":"10.1371\/journal.ppat.1012130"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15957","DOI":"10.1021\/acsomega.1c01626","article-title":"HIV Replication Is Increased by RNA Methylation METTL3\/METTL14\/WTAP Complex Activators","volume":"6","author":"Selberg","year":"2021","journal-title":"ACS Omega"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature12730","article-title":"N6-methyladenosine-dependent regulation of messenger RNA stability","volume":"505","author":"Wang","year":"2014","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/j.cell.2015.05.014","article-title":"N6-methyladenosine Modulates Messenger RNA Translation Efficiency","volume":"161","author":"Wang","year":"2015","journal-title":"Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.molcel.2019.04.025","article-title":"Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers","volume":"74","author":"Shi","year":"2019","journal-title":"Mol. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1038\/s41422-018-0040-8","article-title":"Dynamic transcriptomic m6A decoration: Writers, erasers, readers and functions in RNA metabolism","volume":"28","author":"Yang","year":"2018","journal-title":"Cell Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e0009825","DOI":"10.1128\/jvi.00098-25","article-title":"METTL3 regulates PRRSV replication by suppressing interferon beta through autophagy-mediated IKK\u03b5 degradation","volume":"99","author":"Zhai","year":"2025","journal-title":"J. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e2409132121","DOI":"10.1073\/pnas.2409132121","article-title":"Lactylation of RNA m6A demethylase ALKBH5 promotes innate immune response to DNA herpesviruses and mpox virus","volume":"121","author":"Li","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/s41590-018-0275-z","article-title":"m6A modification controls the innate immune response to infection by targeting type I interferons","volume":"20","author":"Winkler","year":"2019","journal-title":"Nat. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"114905","DOI":"10.1016\/j.intimp.2025.114905","article-title":"METTL3-mediated m6A modification of circCSDE1 promote Coxsackievirus replication by regulating the miR-891b\/BAG3 axis","volume":"159","author":"Wang","year":"2025","journal-title":"Int. Immunopharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1126\/science.aax4468","article-title":"N6-methyladenosine RNA modification-mediated cellular metabolism rewiring inhibits viral replication","volume":"365","author":"Liu","year":"2019","journal-title":"Science"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Banhart, S., Schafer, E.K., Gensch, J.M., and Heuer, D. (2019). Sphingolipid Metabolism and Transport in Chlamydia trachomatis and Chlamydia psittaci Infections. Front. Cell Dev. Biol., 7.","DOI":"10.3389\/fcell.2019.00223"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5201","DOI":"10.1038\/ncomms6201","article-title":"Chlamydia infection depends on a functional MDM2-p53 axis","volume":"5","author":"Gonzalez","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.celrep.2014.10.004","article-title":"Tumor suppressor p53 alters host cell metabolism to limit Chlamydia trachomatis infection","volume":"9","author":"Siegl","year":"2014","journal-title":"Cell Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1038\/s41564-020-0762-5","article-title":"Reprogramming of host glutamine metabolism during Chlamydia trachomatis infection and its key role in peptidoglycan synthesis","volume":"5","author":"Rajeeve","year":"2020","journal-title":"Nat. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/5\/1025\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:26:03Z","timestamp":1778905563000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/5\/1025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["microorganisms14051025"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14051025","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]}}}