{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T09:15:03Z","timestamp":1778145303808,"version":"3.51.4"},"reference-count":72,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio del Poder Popular de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n of Venezuela","award":["324-2024"],"award-info":[{"award-number":["324-2024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Hepatitis E virus (HEV) is an emerging zoonotic pathogen of increasing concern in developed regions and represents a major cause of acute viral hepatitis worldwide, primarily transmitted via the fecal\u2013oral route. Although most infections are self-limiting, immunocompromised individuals, such as people living with human immunodeficiency virus (PLWH) and pregnant women, are at risk of severe outcomes, including chronic infection and fatal liver failure, respectively. This study was aimed at evaluating the prevalence and genetic diversity of HEV in PLWH and relevant ecological niches (swine and wastewater) in Venezuela. A total of 417 serum samples from PLWH, 85 wastewater samples, and 67 swine fecal samples were tested for serological or molecular HEV markers. The seroprevalence of anti-HEV antibodies among PLWH was 0.2% for IgM and 5.5% for IgG. HEV RNA was not detected in samples from PLWH or wastewater; however, a 1.5% prevalence of active infection was identified in swine. Phylogenetic analysis of a complete HEV genome revealed an unassignable subtype within genotype 3, tentatively designated as 3p. To the best of our knowledge, this study provides the first molecular characterization and report on HEV frequency in PLWH, wastewater, and swine in Venezuela.<\/jats:p>","DOI":"10.3390\/microorganisms14051045","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T08:01:39Z","timestamp":1778140899000},"page":"1045","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A One Health Approach to Hepatitis E Virus in Venezuela: Low Seroprevalence in Humans and First Genomic Evidence of Hepatitis E Virus Genotype 3 in a Domestic Swine"],"prefix":"10.3390","volume":"14","author":[{"given":"Julie Andreina","family":"Beltr\u00e1n","sequence":"first","affiliation":[{"name":"Laboratorio de Virolog\u00eda Molecular, Centro de Microbiolog\u00eda y Biolog\u00eda Celular (CMBC), Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas 1020-A, Venezuela"},{"name":"Unidad de Microscop\u00eda Electr\u00f3nica, Centro de Qu\u00edmica, Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas 1020-A, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoneira Fabiola","family":"Sulbar\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Molecular, Centro de Microbiolog\u00eda y Biolog\u00eda Celular (CMBC), Instituto Venezolano de Investigaciones Cient\u00edficas (IVIC), Caracas 1020-A, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5139-0609","authenticated-orcid":false,"given":"Lily","family":"Soto","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Inmunolog\u00eda, Instituto de Medicina Tropical, Universidad Central de Venezuela (UCV), Caracas 1050, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Unidad de Infectolog\u00eda, Hospital Dr. Jes\u00fas Yerena, L\u00eddice, Caracas 1010, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Comegna","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Proyecto Once Trece, Caracas 1060, Venezuela"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Graciela","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Unidad de VIH, Servicio de Enfermedades Infecciosas, Hospital de Ni\u00f1os J. 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