{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:27:12Z","timestamp":1785403632295,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009088","name":"University of Veterinary Medicine Vienna","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009088","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01w6qp003","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Mycoplasmas are frequently recovered from the upper respiratory tract of birds of prey, yet many isolates remain taxonomically unresolved. In the present study, a collection of ten previously unclassified Mycoplasma strains, predominantly isolated from the trachea of the common buzzard (Buteo buteo), was subjected to comprehensive phenotypic and genomic characterization. All strains grew well in modified Hayflick\u2019s medium and formed colonies with the characteristic fried-egg appearance. None of the strains produced acid from glucose or hydrolyzed arginine or urea. Phylogenetic analyses based on 16S rRNA gene, 16S\u201323S intergenic spacer, and partial rpoB gene sequences placed the strains within the Mycoplasma synoviae cluster, in close proximity to five recently described Mycoplasma species associated with raptors such as eagles and kites. Matrix-assisted laser desorption ionization\u2013time of flight (MALDI-ToF) mass spectrometry enabled the clear discrimination of the investigated strains from closely related taxa. Whole-genome comparisons, together with phylogenomic analyses, supported the assignment of these strains to a novel species within the genus Mycoplasma. The name Mycoplasma tracheobuteonis sp. nov. is proposed, corresponding to its preference for colonizing the upper respiratory tract of the common buzzard, with strain 48589BT (=DSM 115882T = NCTC 14927T) designated as the type strain.<\/jats:p>","DOI":"10.3390\/microorganisms14061224","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T10:06:56Z","timestamp":1780049216000},"page":"1224","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mycoplasma tracheobuteonis sp. nov., a Novel Respiratory Mycoplasma Species from the Common Buzzard (Buteo buteo)"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1140-3827","authenticated-orcid":false,"given":"Sarah","family":"Kugler","sequence":"first","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Sciences, University of Veterinary Medicine, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-3835","authenticated-orcid":false,"given":"Anna","family":"K\u00fcbber-Heiss","sequence":"additional","affiliation":[{"name":"Research Institute of Wildlife Ecology, Department of Interdisciplinary Sciences, University of Veterinary Medicine, 1160 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Dinhopl","sequence":"additional","affiliation":[{"name":"Unit Pathology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4790-9823","authenticated-orcid":false,"given":"Angelika","family":"Auer","sequence":"additional","affiliation":[{"name":"Unit Infectiology and Virology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1401-2961","authenticated-orcid":false,"given":"Igor","family":"Loncaric","sequence":"additional","affiliation":[{"name":"Unit Microbiology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6001-9542","authenticated-orcid":false,"given":"Volker","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Clinic for Birds and Reptiles, Faculty of Veterinary Medicine, University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8721-775X","authenticated-orcid":false,"given":"Ana S.","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Sanidad Animal y Seguridad Alimentaria, University of Las Palmas de Gran Canaria, 35413 Arucas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0164-0179","authenticated-orcid":false,"given":"Joachim","family":"Spergser","sequence":"additional","affiliation":[{"name":"Unit Microbiology, Department of Biological Sciences and Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1128\/MMBR.62.4.1094-1156.1998","article-title":"Molecular Biology and Pathogenicity of Mycoplasmas","volume":"62","author":"Razin","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Balish, M.F., Chopra-Dewasthaly, R., Pereyre, S., Ram\u00edrez, A.S., Viver, T., and Spergser, J. (2024). Mycoplasma. Bergey\u2019s Manual of Systematics of Archaea and Bacteria, John Wiley & Sons, Inc.","DOI":"10.1002\/9781118960608.gbm01263.pub3"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1080\/00071660500066282","article-title":"Poultry Mycoplasmas: Sophisticated Pathogens in Simple Guise","volume":"46","author":"Bradbury","year":"2005","journal-title":"Br. Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Gr\u00f3zner, D., Sulyok, K.M., Kreizinger, Z., R\u00f3nai, Z., J\u00e1nosi, S., Turcs\u00e1nyi, I., K\u00e1rolyi, H.F., Kov\u00e1cs, \u00c1.B., Kiss, M.J., and Volokhov, D. (2019). Detection of Mycoplasma anatis, M. anseris, M. cloacale and Mycoplasma sp. 1220 in Waterfowl Using Species-Specific PCR Assays. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0219071"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1637\/8094-082107-Reg","article-title":"Species-Specific Polymerase Chain Reactions for the Detection of Mycoplasma buteonis, Mycoplasma falconis, Mycoplasma gypis, and Mycoplasma corogypsi in Captive Birds of Prey","volume":"52","author":"Lierz","year":"2008","journal-title":"Avian Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"845","DOI":"10.7589\/0090-3558-44.4.845","article-title":"Occurrence of Mycoplasmas in Free-Ranging Birds of Prey in Germany","volume":"44","author":"Lierz","year":"2008","journal-title":"J. Wildl. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1637\/8378-061608-Reg.1","article-title":"The Role of Mycoplasmas in a Conservation Project of the Lesser Kestrel (Falco naumanni)","volume":"52","author":"Lierz","year":"2008","journal-title":"Avian Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1099\/00207713-44-1-94","article-title":"Mycoplasma buteonis sp. nov., Mycoplasma falconis sp. nov., and Mycoplasma gypis sp. Nov., Three Species from Birds of Prey","volume":"44","author":"Poveda","year":"1994","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1099\/ijs.0.033803-0","article-title":"Mycoplasma neophronis sp. nov., Isolated from the Upper Respiratory Tract of Canarian Egyptian Vultures (Neophron percnopterus majorensis)","volume":"62","author":"Rosales","year":"2012","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5909","DOI":"10.1128\/JCM.42.12.5909-5912.2004","article-title":"Identification of a Novel Mycoplasma Species from an Oriental White-Backed Vulture (Gyps bengalensis)","volume":"42","author":"Oaks","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1099\/ijsem.0.003235","article-title":"Mycoplasma hafezii Sp. Nov., Isolated from the Trachea of a Peregrine Falcon (Falco peregrinus)","volume":"69","author":"Ziegler","year":"2019","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"108789","DOI":"10.1016\/j.vetmic.2020.108789","article-title":"Description, Occurrence and Significance of Mycoplasma seminis sp. Nov. Isolated from Semen of a Gyrfalcon (Falco rusticolus)","volume":"247","author":"Fischer","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1099\/00207713-43-3-585","article-title":"Mycoplasma corogypsi sp. Nov., a New Species from the Footpad Abscess of a Black Vulture, Coragyps Atratus","volume":"43","author":"Panangala","year":"1993","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"808","DOI":"10.7589\/0090-3558-45.3.808","article-title":"Association of Mycoplasma Corogypsi and Polyarthritis in a Black Vulture (Coragyps atratus) in Virginia","volume":"45","author":"Ruder","year":"2009","journal-title":"J. Wildl. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1177\/0300985812457791","article-title":"Mycoplasma Corogypsi Associated Polyarthritis and Tenosynovitis in Black Vultures (Coragyps atratus)","volume":"50","author":"Ley","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"159","DOI":"10.7589\/0090-3558-44.1.159","article-title":"Isolation and Characterization of Unusual Mycoplasma spp. from Captive Eurasian Griffon (Gyps fulvus) in Sicily","volume":"44","author":"Loria","year":"2008","journal-title":"J. Wildl. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.rvsc.2009.12.016","article-title":"Identification and Characterization of Novel Mycoplasma spp. Belonging to the Hominis Group from Griffon Vultures","volume":"89","author":"Lecis","year":"2010","journal-title":"Res. Vet. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1638\/2015-0259.1","article-title":"Molecular Identification and Sequence Characterization of Mycoplasmas in Free-Living Birds of Prey","volume":"47","author":"Lecis","year":"2016","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"17065","DOI":"10.1038\/s41598-021-96577-0","article-title":"Occurrence of Mycoplasma spp. in Wild Birds: Phylogenetic Analysis and Potential Factors Affecting Distribution","volume":"11","author":"Kursa","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"126663","DOI":"10.1016\/j.syapm.2025.126663","article-title":"Mycoplasma aquilae sp. nov., Mycoplasma paraquilae sp. nov., Mycoplasma haliaeeti sp. nov., Mycoplasma milvi sp. nov., and Mycoplasma razini sp. nov., Isolated from Predatory Birds of the Accipitridae Family","volume":"48","author":"Spergser","year":"2025","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10.1128","DOI":"10.1128\/JCM.00316-19","article-title":"Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry Is a Superior Diagnostic Tool for the Identification and Differentiation of Mycoplasmas Isolated from Animals","volume":"57","author":"Spergser","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2703","DOI":"10.1099\/ijs.0.64722-0","article-title":"Revised Minimal Standards for Description of New Species of the Class Mollicutes (Division Tenericutes)","volume":"57","author":"Brown","year":"2007","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_23","unstructured":"Razin, S., and Tully, J.G. (1995). Culture Medium Formulation for Primary Isolation and Maintenance of Mollicutes. Molecular and Diagnostic Procedures in Mycoplasmology, Academic Press."},{"key":"ref_24","unstructured":"Razin, S., and Tully, J.G. (1983). Cloning and Filtration Techniques for Mycoplasmas. Methods in Mycoplasmology, Academic Press."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s13567-014-0099-2","article-title":"First Identification of Proteins Involved in Motility of Mycoplasma gallisepticum","volume":"45","author":"Indikova","year":"2014","journal-title":"Vet. Res."},{"key":"ref_26","unstructured":"Stackebrandt, E., and Goodfellow, M. (1991). 16S\/23S rRNA Sequencing. Nucleic Acid Techniques in Bacterial Systematics, Wiley."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1099\/mic.0.26629-0","article-title":"A Putative Transposase Gene in the 16S\u201323S rRNA Intergenic Spacer Region of Mycoplasma Imitans","volume":"150","author":"Harasawa","year":"2004","journal-title":"Microbiology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1099\/ijsem.0.001755","article-title":"Introducing EzBioCloud: A Taxonomically United Database of 16S rRNA Gene Sequences and Whole-Genome Assemblies","volume":"67","author":"Yoon","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools","volume":"25","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent Updates to the Phylogenetic Tree Display and Annotation Tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_33","first-page":"35","article-title":"Standardized Bacteriologic Techniques for the Characterization of Mycoplasma Species","volume":"20","author":"Aluotto","year":"1970","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_34","unstructured":"Razin, S., and Tully, J.G. (1983). Haemagglutination, Haemadsorption and Haemolysis. Methods in Mycoplasmology, Academic Press."},{"key":"ref_35","unstructured":"Razin, S., and Tully, J.G. (1983). Film and Spot Production. Methods in Mycoplasmology, Academic Press."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Unicycler: Resolving Bacterial Genome Assemblies from Short and Long Sequencing Reads. PLoS Comput. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1093\/bioinformatics\/btv681","article-title":"JSpeciesWS: A Web Server for Prokaryotic Species Circumscription Based on Pairwise Genome Comparison","volume":"32","author":"Richter","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D801","DOI":"10.1093\/nar\/gkab902","article-title":"TYGS and LPSN: A Database Tandem for Fast and Reliable Genome-Based Classification and Nomenclature of Prokaryotes","volume":"50","author":"Carbasse","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML Version 8: A Tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies","volume":"30","author":"Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1101\/gr.2289704","article-title":"Mauve: Multiple Alignment of Conserved Genomic Sequence With Rearrangements","volume":"14","author":"Darling","year":"2004","journal-title":"Genome Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.biologicals.2009.11.008","article-title":"Highlights of Mycoplasma Research\u2014An Historical Perspective","volume":"38","author":"Razin","year":"2010","journal-title":"Biologicals"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.ympev.2011.11.002","article-title":"RNA Polymerase Beta Subunit (rpoB) Gene and the 16S\u201323S rRNA Intergenic Transcribed Spacer Region (ITS) as Complementary Molecular Markers in Addition to the 16S rRNA Gene for Phylogenetic Analysis and Identification of the Species of the Family Mycoplasmataceae","volume":"62","author":"Volokhov","year":"2012","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"19126","DOI":"10.1073\/pnas.0906412106","article-title":"Shifting the Genomic Gold Standard for the Prokaryotic Species Definition","volume":"106","author":"Richter","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Citti, C., Baranowski, E., Dordet-Frisoni, E., Faucher, M., and Nouvel, L.-X. (2020). Genomic Islands in Mycoplasmas. Genes, 11.","DOI":"10.3390\/genes11080836"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3","DOI":"10.21775\/cimb.029.003","article-title":"Horizontal Gene Transfers in Mycoplasmas (Mollicutes)","volume":"29","author":"Citti","year":"2018","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"eabf2403","DOI":"10.1126\/sciadv.abf2403","article-title":"The Mycoplasma Surface Proteins MIB and MIP Promote the Dissociation of the Antibody-Antigen Interaction","volume":"7","author":"Nottelet","year":"2021","journal-title":"Sci. Adv."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/6\/1224\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T04:21:24Z","timestamp":1780201284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/6\/1224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,29]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["microorganisms14061224"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14061224","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,29]]}}}