{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:53:33Z","timestamp":1790056413254,"version":"4.0.1"},"reference-count":37,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cell wall mannan samples were prepared from a single strain of each Candida parapsilosis complex species (C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis) using benanomicin A, an antibiotic with lectin-like activity. Their O-linked oligosaccharides were subsequently obtained in good yield via \u03b2-elimination (alkaline treatment). From the mannan of C. parapsilosis sensu stricto, only short \u03b1-1,2-linked manno-oligosaccharides were obtained. In contrast, the mannans of C. orthopsilosis and C. metapsilosis additionally yielded a unique pentasaccharide, Man\u03b11-2Man\u03b11-3Man\u03b11-2Man\u03b11-2Man. Notably, this pentasaccharide, which contains an internal \u03b1-1,3-linked mannose residue, was recovered in appreciable amounts, whereas the corresponding tetrasaccharide (Man\u03b11-3Man\u03b11-2Man\u03b11-2Man), a presumed biosynthetic intermediate, was barely detectable. This structure represents, to our knowledge, the first example of such a manno-oligosaccharide isolated from the O-linked domain of budding yeast cell wall mannans.<\/jats:p>","DOI":"10.3390\/microorganisms14071472","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T08:05:38Z","timestamp":1783325138000},"page":"1472","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery of an Internal \u03b1-1,3-Linkage-Containing Manno-Oligosaccharide in the O-Linked Cell Wall Mannan of Two Candida parapsilosis Complex Species via a Benanomicin A-Based Purification Method"],"prefix":"10.3390","volume":"14","author":[{"given":"Kei","family":"Higashijima","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-4693","authenticated-orcid":false,"given":"Takuya","family":"Kuraoka","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiko","family":"Fujimatsu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Ambo","sequence":"additional","affiliation":[{"name":"Center for Laboratory Animal Science, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Miyagi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumie","family":"Ito","sequence":"additional","affiliation":[{"name":"Division of Infection and Host Defense, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Miyagi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1487-8079","authenticated-orcid":false,"given":"Yukiko","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory of Infection Control, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemitsu","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1128\/JCM.43.1.284-292.2005","article-title":"Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III","volume":"43","author":"Tavanti","year":"2005","journal-title":"J. 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