{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:23:26Z","timestamp":1785425006221,"version":"3.56.0"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Carlos Chagas Institute"},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"crossref","award":["307067\/2023-8"],"award-info":[{"award-number":["307067\/2023-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"award":["307067\/2023-8"],"award-info":[{"award-number":["307067\/2023-8"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This study describes the management practices and evaluates the molecular identification of Cryptosporidium species in dairy calves from southern Santa Catarina, Brazil, an important dairy cattle milk production region. Fecal diarrhea samples from 67 calves aged 1 to 90 days across 22 farms were analyzed by nested PCR for Cryptosporidium DNA detection. Sequencing 24 PCR-positive samples from 14 farms revealed the presence of Cryptosporidium parvum, demonstrating the circulation of the species in the farms. Phylogenetic analysis confirmed high sequence similarity (99.01\u2013100%) with reference strains, indicating genetic diversity and homogeneity in infection dynamics. The identification of C. parvum, a zoonotic species, raises significant concerns for animal and public health. This research represents molecular evidence of the Cryptosporidium parvum in diarrheic dairy calves from farms in the southern part of Santa Catarina, Brazil, underscoring the need for targeted control strategies and ongoing molecular surveillance to mitigate transmission risks in dairy farming systems.<\/jats:p>","DOI":"10.3390\/microorganisms14071552","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:49:36Z","timestamp":1784184576000},"page":"1552","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Molecular Characterization of Cryptosporidium in Diarrheic Dairy Calves: A Descriptive Study in Family Farms of Southern Santa Catarina, Brazil"],"prefix":"10.3390","volume":"14","author":[{"given":"Guilherme","family":"Drescher","sequence":"first","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Am\u00e9rico","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Parasitic Diseases (LAPAR-CAV-UDESC), Department of Veterinary Medicine, Agroveterinary Sciences Center, Santa Catarina State University, Lages 88520-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Costanski","sequence":"additional","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michail Sabino","family":"Moroz","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science, School of Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna Caroline","family":"Prochno","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science, School of Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba 80215-901, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano Borges","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz), Curitiba 81310-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"ref_1","unstructured":"Statista (2026, January 10). 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