{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T08:05:13Z","timestamp":1784880313471,"version":"3.55.0"},"reference-count":27,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Staphylococcus aureus causes infections ranging from skin and soft tissue infections to life-threatening systemic disease. The convergence of virulence determinants with antimicrobial resistance, particularly in methicillin-resistant S. aureus (MRSA), complicates management. In the United Arab Emirates (UAE), S. aureus is a major contributor to community- and hospital-acquired infections, and regional surveillance from the Gulf Cooperation Council has documented a shift toward community-associated MRSA lineages. Despite this clinical burden, granular molecular epidemiological data from the UAE remain scarce. Fifty consecutive S. aureus clinical isolates were obtained from Thumbay Laboratories, Ajman, UAE (October 2022\u2013August 2023). Identification and antimicrobial susceptibility testing against 23 agents were performed on the MicroScan WalkAway system. Five virulence genes, namely mecA, Panton-Valentine leukocidin (PVL; lukF-S), exfoliative toxins A (eta) and B (etb), and toxic shock syndrome toxin (tst), were detected by conventional PCR. PVL was the most prevalent virulence gene (48%, 24\/50), detected exclusively in pus isolates. mecA carriage, confirming MRSA, was identified in 44% (22\/50), with PVL\u2013mecA co-occurrence in 22% (11\/50). eta was present in 6% (3\/50), and etb and tst each in 2% (1\/50). Resistance was highest for ampicillin and penicillin (86% each), while full susceptibility (100%) was retained for vancomycin, linezolid, daptomycin, teicoplanin, rifampin, Synercid, and trimethoprim. mecA carriage was significantly associated with resistance to amoxicillin, clavulanate, ampicillin, imipenem, penicillin, oxacillin, azithromycin, erythromycin, and ciprofloxacin (p &lt; 0.05). This study provides the first integrated molecular characterization of key S. aureus virulence determinants, along with a comprehensive antimicrobial susceptibility profile in the UAE. The high PVL prevalence and substantial MRSA burden highlight the need for structured regional surveillance and antimicrobial stewardship, while retained susceptibility to last-resort agents offer actionable guidance for empirical therapy.<\/jats:p>","DOI":"10.3390\/microorganisms14081613","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T07:36:13Z","timestamp":1784878573000},"page":"1613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Virulence Gene Profile and Antimicrobial Susceptibility of Staphylococcus aureus Clinical Isolates from the United Arab Emirates"],"prefix":"10.3390","volume":"14","author":[{"given":"Wejdan","family":"Alamodi","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6149-0467","authenticated-orcid":false,"given":"Sara","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5692-1632","authenticated-orcid":false,"given":"Marwan","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2669-4488","authenticated-orcid":false,"given":"Praveen","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6439-5062","authenticated-orcid":false,"given":"Salma Elnour Rahma","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121332","DOI":"10.1016\/j.lfs.2022.121332","article-title":"Recent advancement, immune responses, and mechanism of action of various vaccines against intracellular bacterial infections","volume":"314","author":"Ali","year":"2022","journal-title":"Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"GPP3-0046-2018","DOI":"10.1128\/microbiolspec.GPP3-0046-2018","article-title":"Surface proteins of Staphylococcus aureus","volume":"7","author":"Foster","year":"2019","journal-title":"Microbiol. Spectr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1167\/iovs.12-11276","article-title":"lukSF-PV in Staphylococcus aureus keratitis isolates and association with clinical outcome","volume":"54","author":"Sueke","year":"2013","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Vittorakis, E., Vic\u0103, M.L., Zervaki, C.O., Vittorakis, E., Maraki, S., Mavromanolaki, V.E., Sch\u00fcrger, M.E., Neculicioiu, V.S., Papadomanolaki, E., and Sinanis, T. (2023). Examining the prevalence and antibiotic susceptibility of S. aureus strains in hospitals: An analysis of the pvl gene and its co-occurrence with other virulence factors. Microorganisms, 11.","DOI":"10.3390\/microorganisms11040841"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Mariutti, R.B., Tartaglia, N.R., Seyffert, N., de Paula Castro, T.L., Arni, R.K., Azevedo, V.A., Le Loir, Y., and Nishifuji, K. (2017). Exfoliative toxins of Staphylococcus aureus. The Rise of Virulence and Antibiotic Resistance in S. aureus, IntechOpen.","DOI":"10.5772\/66528"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"465","DOI":"10.36103\/ijas.v53i2.1554","article-title":"Prevalence of exfoliative toxin genes among clinical isolates of Staphylococcus aureus in Iraq","volume":"53","author":"Yassin","year":"2022","journal-title":"Iraqi J. Agric. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.3390\/toxins5091629","article-title":"Update on staphylococcal superantigen-induced signaling pathways and therapeutic interventions","volume":"5","author":"Krakauer","year":"2013","journal-title":"Toxins"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Azzam, A., Khaled, H., Mosa, M., Refaey, N., AlSaifi, M., Elsisi, S., Elagezy, F.K., and Mohsen, M. (2023). Epidemiology of clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) and its susceptibility to linezolid and vancomycin in Egypt: A systematic review with meta-analysis. BMC Infect. Dis., 23.","DOI":"10.1186\/s12879-023-08202-2"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1007\/s00284-021-02442-8","article-title":"Antibiotic resistance crisis: An Update on antagonistic interactions between probiotics and methicillin-resistant Staphylococcus aureus (MRSA)","volume":"78","author":"Nataraj","year":"2021","journal-title":"Curr. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"434","DOI":"10.26719\/emhj.22.042","article-title":"Trends in methicillin-resistant Staphylococcus aureus in the Gulf Cooperation Council countries: Antibiotic resistance, virulence factors and emerging strains","volume":"28","author":"Shahid","year":"2022","journal-title":"East. Mediterr. Health J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Thomsen, J., Abdulrazzaq, N.M., The UAE AMR Surveillance Consortium, Menezes, G.A., Ayoub Moubareck, C., Everett, D.B., and Senok, A. (2023). Methicillin resistant Staphylococcus aureus in the United Arab Emirates: A 12-year retrospective analysis of evolving trends. Front. Public Health, 11.","DOI":"10.3389\/fpubh.2023.1244351"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Briki, A., Alkhatib, N., Aloba, B., Verma, S., Nassar, R., Everett, D., Ehricht, R., Monecke, S., and Senok, A. (2025). Molecular epidemiology of methicillin-resistant Staphylococcus aureus in Gulf Cooperation Council countries (2010\u20132025): A scoping review. Front. Microbiol., 16, Erratum in Front. Microbiol. 2026, 17, 1791202. https:\/\/doi.org\/10.3389\/fmicb.2026.1791202.","DOI":"10.3389\/fmicb.2025.1714252"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2753992","DOI":"10.1155\/cjid\/2753992","article-title":"Understanding the molecular epidemiology of community-acquired methicillin-resistant Staphylococcus aureus in northern Saudi Arabia: A spotlight on SCCmec and spa typing","volume":"2025","author":"Alanazi","year":"2025","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_14","unstructured":"(2022). Performance Standards for Antimicrobial Susceptibility Testing (Standard No. CLSI M100)."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e11341","DOI":"10.5812\/jjm.11341","article-title":"Methicillin-resistant Staphylococcus aureus isolated from various types of hospital infections in pediatrics: Panton-valentine leukocidin, staphylococcal chromosomal cassette mec SCCmec phenotypes and antibiotic resistance properties","volume":"8","author":"Dormanesh","year":"2015","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Budzy\u0144ska, A., Skowron, K., Kaczmarek, A., Wietlicka-Piszcz, M., and Gospodarek-Komkowska, E. (2021). Virulence factor genes and antimicrobial susceptibility of Staphylococcus aureus Strains Isolated from blood and chronic wounds. Toxins, 13.","DOI":"10.3390\/toxins13070491"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.12688\/f1000research.15484.1","article-title":"Characterization of Panton-Valentine leukocidin-positive Staphylococcus aureus from skin and soft tissue infections in Lagos, Nigeria","volume":"7","author":"Ayepola","year":"2018","journal-title":"F1000Research"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jiph.2013.11.003","article-title":"Prevalence of exfoliative toxin A and B genes in Staphylococcus aureus isolated from clinical specimens","volume":"7","author":"Koosha","year":"2014","journal-title":"J. Infect. Public Health"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Goudarzi, M., Goudarzi, H., Am, S.F., Udo, E.E., Fazeli, M., Asadzadeh, M., and Seyedjavadi, S.S. (2016). Molecular characterization of methicillin resistant Staphylococcus aureus strains isolated from intensive care units in Iran: ST22-SCCmec IV\/t790 emerges as the major clone. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0155529"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3329\/bjm.v38i1.55532","article-title":"Antibiotic sensitivity and virulence genes in Staphylococcus aureus isolates from surgical site infection","volume":"38","author":"Akhter","year":"2021","journal-title":"Bangladesh J. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"491","DOI":"10.33073\/pjm-2022-042","article-title":"Screening of toxin genes in Methicillin-Resistant Staphylococcus aureus clinical isolates from a Hospital Setting in a tertiary hospital in Northern Cyprus","volume":"71","author":"Potindji","year":"2022","journal-title":"Pol. J. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Xie, X., Bao, Y., Ouyang, N., Dai, X., Pan, K., Chen, B., Deng, Y., Wu, X., Xu, F., and Li, H. (2016). Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolatesMRSA in Sun Yat-sen Memorial Hospital, Guangzhou, Southern China. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1684-y"},{"key":"ref_23","first-page":"e10741","article-title":"Analysis of virulence genes among Methicillin Resistant Staphylococcus aureus (MRSA) strains","volume":"7","author":"Alfatemi","year":"2014","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e39349","DOI":"10.5812\/pedinfect.39349","article-title":"Distribution of adhesion and toxin genes in Staphylococcus aureus strains recovered from Hospitalized Patients Admitted to the ICU","volume":"5","author":"Eftekhar","year":"2016","journal-title":"Arch. Pediatr. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bhowmik, D., Chetri, S., Das, B.J., Chanda, D.D., and Bhattacharjee, A. (2021). Distribution of virulence genes and SCCmec types among methicillin-resistant Staphylococcus aureus of clinical and environmental origin: A study from community of Assam, India. BMC Res. Notes, 14.","DOI":"10.1186\/s13104-021-05473-3"},{"key":"ref_26","first-page":"E202","article-title":"Detection of the tst gene and determining the relationship between the presence of the mecA gene and antibiotic resistance of Staphylococcus aureus isolated from some hospital in Dhi Qar province","volume":"30","year":"2023","journal-title":"J. Popul. Ther. Clin. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1093\/infdis\/jit129","article-title":"Effects of linezolid on suppressing in vivo production of staphylococcal toxins and improving survival outcomes in a rabbit model of methicillin-resistant Staphylococcus aureus necrotizing pneumonia","volume":"208","author":"Diep","year":"2013","journal-title":"J. Infect. Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1613\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T07:52:06Z","timestamp":1784879526000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,24]]},"references-count":27,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081613"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081613","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,24]]}}}