{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T04:31:34Z","timestamp":1786422694617,"version":"build-2736575974"},"reference-count":72,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000277","name":"Department for Environment Food and Rural Affairs","doi-asserted-by":"publisher","award":["CR2000A"],"award-info":[{"award-number":["CR2000A"]}],"id":[{"id":"10.13039\/501100000277","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CR2000A"],"award-info":[{"award-number":["CR2000A"]}],"id":[{"id":"https:\/\/ror.org\/00tnppw48","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Hatcheries potentially can disseminate Salmonella through poultry production chains. This study describes the investigation and control of persistent Salmonella enterica serovar 13,23:i:- in a UK broiler hatchery. Over four years from 2015 to 2019, a broiler hatchery was sampled intensively seven times, yielding Salmonella from 361 of 2645 samples. The findings assisted biosecurity advice and the targeting both of cleaning and disinfection (C&amp;D) and subsequent sampling rounds. Additional sampling was conducted to assess C&amp;D and monitor antimicrobial resistance, and also among all the supplying breeder flocks. The large majority of the isolates were Salmonella 13,23:i:- and the degree of contamination increased along the process pathway. The egg areas and the setter incubators were the least contaminated, whilst the hatcher cabinets, the chick handling areas, and the waste and external areas yielded Salmonella most frequently. The likely resident serovar 13,23:i:- was not eradicated during this study, but diligent management and C&amp;D enhancements and targeting, plus effective biosecurity, were eventually followed by a substantial reduction in Salmonella contamination. The percentage of positive samples per visit reduced to 2.3% from a mid-study peak of 23%. The antimicrobial resistance was varied, infrequent (detected in 4% of tested isolates), and did not show longitudinal trends. This study shows that long-term Salmonella hatchery contamination may be susceptible to targeted interventions, as informed by repeated sampling, provided that biosecurity is of a high standard and C&amp;D (including biofilm disruption) has been optimised. Importantly, it also illustrates the long timescale over which interventions may be required to reduce resident Salmonella contamination.<\/jats:p>","DOI":"10.3390\/microorganisms14081747","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T01:20:03Z","timestamp":1786324803000},"page":"1747","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Salmonella enterica 13,23:i:- in a UK Broiler Hatchery: A Longitudinal Case Study of the Distribution of Resident Contamination and Its Control"],"prefix":"10.3390","volume":"14","author":[{"given":"Kate","family":"Newton","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone KT15 3NB, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8657-3007","authenticated-orcid":false,"given":"Andrew","family":"Wales","sequence":"additional","affiliation":[{"name":"Discipline of Comparative Biomedical Sciences, School of Veterinary Medicine, University of Surrey, Guildford GU2 7AL, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5095-5599","authenticated-orcid":false,"given":"Christopher","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone KT15 3NB, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9799-4085","authenticated-orcid":false,"given":"Claire","family":"Oastler","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone KT15 3NB, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4934-5272","authenticated-orcid":false,"given":"Francesca","family":"Martelli","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone KT15 3NB, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Animal and Plant Health Agency, Addlestone KT15 3NB, Surrey, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"EFSA, and ECDC (2024). The European Union One Health 2023 Zoonoses report. EFSA J., 22, e9106.","DOI":"10.2903\/j.efsa.2024.9106"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.4315\/0362-028X-58.3.326","article-title":"Health risks and consequences of Salmonella and Campylobacter jejuni in raw poultry","volume":"58","author":"Bryan","year":"1995","journal-title":"J. Food Prot."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102566","DOI":"10.1016\/j.psj.2023.102566","article-title":"A systematic review and meta-analysis of the sources of Salmonella in poultry production (pre-harvest) and their relative contributions to the microbial risk of poultry meat","volume":"102","author":"Wang","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1089\/fpd.2014.1744","article-title":"Source attribution of human salmonellosis: An overview of methods and estimates","volume":"11","author":"Pires","year":"2014","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"155","DOI":"10.4142\/jvs.2007.8.2.155","article-title":"Dissemination and tracking of Salmonella spp. in integrated broiler operation","volume":"8","author":"Kim","year":"2007","journal-title":"J. Vet. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Gosling, R., Oastler, C., Nichols, C., Jackson, G., Wales, A.D., and Davies, R.H. (2022). Investigations into Salmonella contamination in feed mills producing rations for the broiler industry in Great Britain. Vet. Sci., 9.","DOI":"10.3390\/vetsci9070307"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1136\/vr.149.8.227","article-title":"Observations on the distribution and control of Salmonella species in two integrated broiler companies","volume":"149","author":"Davies","year":"2001","journal-title":"Vet. Rec."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13028-017-0281-4","article-title":"Occurrence of Salmonella spp.: A comparison between indoor and outdoor housing of broilers and laying hens","volume":"59","author":"Wierup","year":"2017","journal-title":"Acta Vet. Scand."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1080\/0307945031000097822","article-title":"Investigation of the distribution and control of Salmonella enterica serovar Enteritidis PT6 in layer breeding and egg production","volume":"32","author":"Davies","year":"2003","journal-title":"Avian Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0378-1135(97)00157-0","article-title":"Bacteriological and serological investigation of persistent Salmonella enteritidis infection in an integrated poultry organisation","volume":"58","author":"Davies","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Volkova, V.V., Bailey, R.H., and Wills, R.W. (2009). Salmonella in broiler litter and properties of soil at farm location. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006403"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1080\/00439339.2020.1789533","article-title":"Review of hatchery transmission of bacteria with focus on Salmonella, chick pathogens and antimicrobial resistance","volume":"76","author":"Wales","year":"2020","journal-title":"Worlds Poult. Sci. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105492","DOI":"10.1016\/j.prevetmed.2021.105492","article-title":"Risk factor analysis for Salmonella contamination of broiler chicken (Gallus gallus) hatcheries in Great Britain","volume":"196","author":"Withenshaw","year":"2021","journal-title":"Prev. Vet. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/zph.12938","article-title":"Observations on the distribution and control of Salmonella in commercial broiler hatcheries in Great Britain","volume":"69","author":"Oastler","year":"2022","journal-title":"Zoonoses Public Health"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.4315\/0362-028X-64.11.1690","article-title":"Sources and movement of Salmonella through integrated poultry operations: A multistate epidemiological investigation","volume":"64","author":"Bailey","year":"2001","journal-title":"J. Food Prot."},{"key":"ref_16","unstructured":"Racicot, M., Leroux, A., Ng, S., Comeau, G., Quessy, S.G., and Gaucher, M.-L. (2018, January 25\u201327). The Canadian food inspection agency establishment-based risk assessment model for hatcheries: Principles and algorithm. Proceedings of the IAFP European Symposium on Food Safety, Stockholm, Sweden."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"EFSA (2009). Quantitative estimation of the impact of setting a new target for the reduction of Salmonella in breeding hens of Gallus gallus. EFSA J., 7, e1036.","DOI":"10.2903\/j.efsa.2009.1036"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"EFSA, BIOHAZ, Koutsoumanis, K., Allende, A., Alvarez-Ord\u00f3\u00f1ez, A., Bolton, D., Bover-Cid, S., Chemaly, M., De Cesare, A., and Herman, L. (2019). Salmonella control in poultry flocks and its public health impact. EFSA J., 17, e5596.","DOI":"10.2903\/j.efsa.2019.5596"},{"key":"ref_19","first-page":"1","article-title":"A temporal study of Salmonella serovars from environmental samples from poultry breeder flocks in Ontario between 1998 and 2008","volume":"77","author":"Sivaramalingam","year":"2013","journal-title":"Can. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3184\/175815509X431885","article-title":"Hygiene and microbiological control in hatcheries","volume":"2","author":"McMullin","year":"2009","journal-title":"Avian Biol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/03079459708419202","article-title":"Hatchery-borne Salmonella enterica serovar Tennessee infections in broilers","volume":"26","author":"Christensen","year":"1997","journal-title":"Avian Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S12","DOI":"10.1080\/00071660410001697994","article-title":"Observations on the distribution and control of Salmonella contamination in poultry hatcheries","volume":"45","author":"Davies","year":"2004","journal-title":"Br. Poult. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.3382\/ps.0691606","article-title":"Presence and impact of Salmonella contamination in commercial broiler hatcheries","volume":"69","author":"Cox","year":"1990","journal-title":"Poult. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e03398-16","DOI":"10.1128\/AEM.03398-16","article-title":"Adaptation of Salmonella enterica serovar Senftenberg to linalool and its association with antibiotic resistance and environmental persistence","volume":"83","author":"Kalily","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1080\/03079457.2016.1146820","article-title":"Observations on the distribution and control of Salmonella in commercial duck hatcheries in the UK","volume":"45","author":"Martelli","year":"2016","journal-title":"Avian Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1111\/j.1863-2378.2012.01524.x","article-title":"Investigations of the distribution and persistence of Salmonella and ciprofloxacin-resistant Escherichia coli in turkey hatcheries in the UK","volume":"60","author":"Clouting","year":"2013","journal-title":"Zoonoses Public Health"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1080\/03079450802216561","article-title":"Persistence of Salmonella Senftenberg in poultry production environments and investigation of its resistance to desiccation","volume":"37","author":"Pedersen","year":"2008","journal-title":"Avian Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100347","DOI":"10.1016\/j.jfp.2024.100347","article-title":"Salmonella biomapping of a commercial broiler hatchery","volume":"87","author":"Rothrock","year":"2024","journal-title":"J. Food Prot."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"583","DOI":"10.2307\/1592082","article-title":"Transmission of Salmonella typhimurium during hatching of broiler chicks","volume":"38","author":"Cason","year":"1994","journal-title":"Avian Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.3382\/ps.0731153","article-title":"Hatchery-acquired salmonellae in broiler chicks","volume":"73","author":"Bailey","year":"1994","journal-title":"Poult. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"103806","DOI":"10.1016\/j.psj.2024.103806","article-title":"Colonization, spread and persistence of Salmonella (Typhimurium, Infantis and Reading) in internal organs of broilers","volume":"103","author":"Wang","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1093\/ps\/79.11.1571","article-title":"Salmonella penetration of egg shells and proliferation in broiler hatching eggs\u2014A review","volume":"79","author":"Cox","year":"2000","journal-title":"Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"365","DOI":"10.20506\/rst.14.2.849","article-title":"Application of disinfectants in poultry hatcheries","volume":"14","author":"Samberg","year":"1995","journal-title":"Rev. Sci. Et Tech. (Int. Off. Epizoot.)"},{"key":"ref_34","first-page":"21","article-title":"The importance of biosecurity in the modern day hatchery","volume":"31","author":"Bennett","year":"2017","journal-title":"Int. Hatch. Pract."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"101223","DOI":"10.1016\/j.animal.2024.101223","article-title":"A conventional hatchery vs \u201con-farm\u201d hatching of broiler chickens in terms of microbiological and microclimatic conditions","volume":"18","author":"Kustra","year":"2024","journal-title":"Animal"},{"key":"ref_36","unstructured":"APHA (2024). Salmonella in Animals and Feed in Great Britain 2023, Animal and Plant Health Agency."},{"key":"ref_37","unstructured":"APHA (2023). Salmonella in Animals and Feed in Great Britain 2022, Animal and Plant Health Agency."},{"key":"ref_38","unstructured":"APHA (2019). Salmonella in Livestock Production in GB, 2018, Animal and Plant Health Agency."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"EFSA, and ECDC (2019). The European Union One Health 2018 Zoonoses Report. EFSA J., 17, e5926.","DOI":"10.2903\/j.efsa.2019.5926"},{"key":"ref_40","unstructured":"PHE (2018). Zoonoses Report UK 2017, Public Health England."},{"key":"ref_41","unstructured":"Grimont, P., and Weill, F. (2007). Antigenic Formulae of the Salmonella serovars, WHO Collaborating Centre for Reference and Research on Salmonella. [9th ed.]."},{"key":"ref_42","unstructured":"VMD (2018). UK Veterinary Antibiotic Resistance and Sales Surveillance, UK-VARSS, Supplementary Material, UK Veterinary Medicines Directorate."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Hosmer, D.W., Lemeshow, S., and Sturdivant, R.X. (2013). Applied Logistic Regression, John Wiley & Sons. [3rd ed.].","DOI":"10.1002\/9781118548387"},{"key":"ref_44","unstructured":"Hensher, D.A., and Stopher, P.R. (1979). Quantitative methods for analysing travel behavior of individuals: Some recent developments. Behavioural Travel Modelling, Croom Helm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/BF02295996","article-title":"Note on the sampling error of the difference between correlated proportions or percentages","volume":"12","author":"McNemar","year":"1947","journal-title":"Psychometrika"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1080\/01621459.1927.10502953","article-title":"Probable inference, the law of succession, and statistical inference","volume":"22","author":"Wilson","year":"1927","journal-title":"J. Am. Stat. Assoc."},{"key":"ref_47","unstructured":"StataCorp (Stata Statistical Software, 2017). Stata Statistical Software, version 15."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13620-018-0114-4","article-title":"Prevalence and characterization of Salmonella in two integrated broiler operations in Korea","volume":"71","author":"Ha","year":"2018","journal-title":"Ir. Vet. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1017\/S0950268816001515","article-title":"Prevalence and molecular characterization of Salmonella enterica isolates throughout an integrated broiler supply chain in China","volume":"144","author":"Ren","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100428","DOI":"10.1016\/j.vas.2025.100428","article-title":"Prevalence, antimicrobial resistance and phylogenetic analysis of Salmonella contamination and transmission in yellow-feathered broiler hatcheries in China","volume":"27","author":"Wu","year":"2025","journal-title":"Vet. Anim. Sci."},{"key":"ref_51","unstructured":"Dunn, A. (Farmers Weekly, 2026). Hatchery Salmonella Outbreak Raises Supply Concerns, Farmers Weekly."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1046\/j.1365-2672.2002.01543.x","article-title":"Sources of Salmonella on broiler carcasses during transportation and processing: Modes of contamination and methods of control","volume":"92","author":"Corry","year":"2002","journal-title":"J. Appl. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1128\/AEM.68.2.713-719.2002","article-title":"Impact of transport crate reuse and of catching and processing on Campylobacter and Salmonella contamination of broiler chickens","volume":"68","author":"Slader","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0168-1605(00)00466-9","article-title":"Biofilm formation by Salmonella spp. on food contact surfaces and their sensitivity to sanitizers","volume":"64","author":"Joseph","year":"2001","journal-title":"Int. J. Food Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"303","DOI":"10.21608\/avmj.2024.293855.1272","article-title":"Evaluation of the tolerance of biofilm forming Salmonella isolated from dead in shell embryos to some disinfectants","volume":"70","author":"Hussein","year":"2024","journal-title":"Assiut Vet. Med. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1093\/ps\/81.6.904","article-title":"Evaluation of chemical disinfectants for the elimination of Salmonella biofilms from poultry transport containers","volume":"81","author":"Ramesh","year":"2002","journal-title":"Poult. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1399\/eps.2017.190","article-title":"Biofilm as risk factor for Salmonella contamination in various stages of poultry production","volume":"81","author":"Milanov","year":"2017","journal-title":"Eur. Poult. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.vetmic.2017.04.004","article-title":"Efficacy of disinfectants and detergents intended for a pig farm environment where Salmonella is present","volume":"204","author":"Gosling","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3139\/113.110632","article-title":"Evaluation of the bactericidal activity of didecyl dimethyl ammonium chloride in 2-propanol against Pseudomonas aeruginosa strains with adaptive resistance to this active substance according to European standards","volume":"56","author":"Chojecka","year":"2019","journal-title":"Tenside Surfact. Deterg."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Aksoy, A., El Kahlout, K., and Yardimci, H. (2020). Comparative evaluation of the effects of binzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide disinfectants against avian salmonellae focusing on genotypic resistance pattern of the salmonellae serotypes toward benzalkonium chloride. Braz. J. Poult. Sci., 22.","DOI":"10.1590\/1806-9061-2019-1055"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1053\/jhin.2001.0950","article-title":"Biocide rotation in the healthcare setting: Is there a case for policy implementation?","volume":"48","author":"Murtough","year":"2001","journal-title":"J. Hosp. Infect."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1093\/jac\/49.4.597","article-title":"Mechanisms of antimicrobial action of antiseptics and disinfectants: An increasingly important area of investigation","volume":"49","author":"Russell","year":"2002","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1128\/AAC.40.4.1009","article-title":"Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals","volume":"40","author":"Griggs","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1128\/JCM.02574-10","article-title":"Nalidixic acid-resistant strains of Salmonella showing decreased susceptibility to fluoroquinolones in the midwestern region of the Republic of Ireland due to mutations in the gyrA gene","volume":"49","author":"Ryan","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_65","unstructured":"APHA (2020). Salmonella in Livestock Production in GB, 2019, Animal and Plant Health Agency."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1089\/fpd.2016.2182","article-title":"Biocide tolerance and antibiotic resistance in Salmonella isolates from hen eggshells","volume":"14","author":"Burgos","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.scitotenv.2017.10.312","article-title":"Antibiotics and common antibacterial biocides stimulate horizontal transfer of resistance at low concentrations","volume":"616\u2013617","author":"Jutkina","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Maertens, H., Demeyere, K., De Reu, K., Dewulf, J., Vanhauteghem, D., Van Coillie, E., and Meyer, E. (2020). Effect of subinhibitory exposure to quaternary ammonium compounds on the ciprofloxacin susceptibility of Escherichia coli strains in animal husbandry. BMC Microbiol., 20.","DOI":"10.1186\/s12866-020-01818-3"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Daehre, K., Projahn, M., Friese, A., Semmler, T., Guenther, S., and Roesler, U.H. (2018). ESBL-producing Klebsiella pneumoniae in the broiler production chain and the first description of ST3128. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02302"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1111\/1751-7915.13040","article-title":"Environmental adaptation and vertical dissemination of ESBL-\/pAmpC-producing Escherichia coli in an integrated broiler production chain in the absence of an antibiotic treatment","volume":"11","author":"Projahn","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/zph.12830","article-title":"Disinfectant testing for veterinary and agricultural applications: A review","volume":"68","author":"Wales","year":"2021","journal-title":"Zoonoses Public Health"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1038\/s41579-023-00958-3","article-title":"Disinfectants and antiseptics: Mechanisms of action and resistance","volume":"22","author":"Maillard","year":"2024","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1747\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T04:18:56Z","timestamp":1786421936000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/14\/8\/1747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,8]]},"references-count":72,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["microorganisms14081747"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms14081747","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,8]]}}}