{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:30:20Z","timestamp":1786365020896,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS (Japan Society for the Promotion of Science) KAKENHI","award":["17780228"],"award-info":[{"award-number":["17780228"]}]},{"name":"JSPS (Japan Society for the Promotion of Science) KAKENHI","award":["20780219"],"award-info":[{"award-number":["20780219"]}]},{"name":"JSPS (Japan Society for the Promotion of Science) KAKENHI","award":["23780299"],"award-info":[{"award-number":["23780299"]}]},{"name":"JSPS (Japan Society for the Promotion of Science) KAKENHI","award":["25450447"],"award-info":[{"award-number":["25450447"]}]},{"name":"JSPS (Japan Society for the Promotion of Science) KAKENHI","award":["24K07444"],"award-info":[{"award-number":["24K07444"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Borna disease virus 1 (BoDV-1) is a noncytolytic, neurotrophic virus that causes neurological disorders, where astrocytes contribute to neuropathology. Cellular prion protein (PrPC) is involved in pro-survival signaling and antioxidative defense. Using rat astroglial C6 cells as an in vitro model, we investigated whether persistent BoDV-1 infection alters N-glycan maturation of PrPC. Western blotting showed that PrPC shifted toward lower-molecular-weight glycoforms as cell density increased. This tendency was more evident in BoDV-1-infected C6 cells without detectable changes in total surface PrPC expression. Two-dimensional polyacrylamide gel electrophoresis showed a shift of PrPC toward lower molecular weight and more basic isoelectric points. Conventional RT-PCR detected reduced signals of MGAT5, which encodes N-acetylglucosaminyltransferase V (GnTV), whereas MGAT1\u20134 signals were not markedly altered. In addition, GnTV overexpression induced a higher-molecular-weight shift of PrPC. PHA-L4 precipitation followed by anti-PrP immunoblotting showed reduced \u03b21-6-branched complex N-glycan signals associated with PrPC. BoDV-1 infection also decreased the RT-PCR signal of E26 transformation-specific 1 (ETS1) as well as reduced ETS-dependent promoter activity and superoxide dismutase activity. These findings suggest that persistent BoDV-1 infection may impair PrPCN-glycan maturation through suppression of the ETS1\u2013MGAT5\/GnTV pathway activity, thereby potentially affecting astroglial antioxidative capacity.<\/jats:p>","DOI":"10.3390\/microorganisms14081759","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T11:38:09Z","timestamp":1786361889000},"page":"1759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["BoDV-1 Infection Is Associated with Altered Prion Protein Glycoform Maturation and Reduced ETS1-MGAT5\/GnTV Pathway Activity in Rat C6 Astroglial Cells"],"prefix":"10.3390","volume":"14","author":[{"given":"Akikazu","family":"Sakudo","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Okayama University of Science, Imabari 794-8555, Ehime, Japan"},{"name":"Research Institute for Microbial Diseases, Osaka University, Yamada-oka, Suita 565-0871, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Ikuta","sequence":"additional","affiliation":[{"name":"Research Institute for Microbial Diseases, Osaka University, Yamada-oka, Suita 565-0871, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13363","DOI":"10.1073\/pnas.95.23.13363","article-title":"Prions","volume":"95","author":"Prusiner","year":"1998","journal-title":"Proc. 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